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Prevalence and Study of the Clonality of Extended-Spectrum Beta-Lactamase-Producing Klebsiella pneumoniae Strains in Neonatology at the University Hospitals of Abidjan by the Pulsed Field Gel Electrophoresis and the Quantitative Antibiogram

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Advances in Microbiology 2024年第9期14卷 416-429页

作者： Valérie M’Bengue Gbonon Sidjè Arlette Afran Stanislas Assohoun Egomli Djeda Franck Arnaud Gnahoré Aboubakar Sylla N’Golo David Coulibaly nathalie guessennd Assanvo Simon-Pierre N’Guetta Mireille Dosso Antibiotics Natural Substances and Anti-Infectious Resistance Surveillance Unit Pasteur Institute of Côte d’Ivoire Abidjan Côte d’Ivoire Molecular Genetics Platform Pasteur Institute of Côte d’Ivoire Abidjan Côte d’Ivoire Genetics and Species Improvement Laboratory Félix Houphouët Boigny University Abidjan Côte d’Ivoire Mechanics and Computer Sciences Laboratory Félix Houphouët Boigny University Abidjan Côte d’Ivoire Biology and Health Laboratory Félix Houphouët Boigny University Abidjan Côte d’Ivoire Molecular Biology Platform Pasteur Institute of Côte d’Ivoire Abidjan Côte d’Ivoire

Background : ESBL-producing strains of Klebsiella pneumoniae , one of the main causes of nosocomial and hospital-acquired infections, are commonly associated with therapeutic impasses. Surveillance of these multidrug-resistant pathogens is a crucial tool for controlling and preventing infections. This surveillance involves the use of appropriate molecular and phenotypic typing techniques. The choice of techniques is based on criteria such as discriminatory power, intra- and inter-laboratory reproducibility, epidemiological concordance, ease of use and cost. The aim of our study was to identify clusters of Extended-Spectrum Beta-Lactamase-producing Klebsiella pneumoniae (ESBL- K. pneumoniae ) strains circulating in neonatology using quantitative antibiogram (QA) and Pulsed Field Gel Electrophoresis (PFGE). Materials and Methods : This cross-sectional study included 55 K. pneumoniae strains isolated from a total of 513 samples. These various samples are taken from newborns, healthcare personnel, and the environment. K. pneu moniae identification followed standard bacteriological procedures and was confirmed using the Vitek® 2 (bioMérieux). The detection of the ESBL phenotype was performed using the synergy test. QA and PFGE were used to identify clonal relationships between the various strains isolated. Concordance between these two methods was assessed by calculating Cohen’s KAPPA coefficient and Simpson’s diversity index. Results : Among the 55 K. pneumoniae strains included in this study, 58.2% (32/55) were found to be Extended-Spectrum Beta-Lactamase (ESBL) producers. Most of these strains were isolated from neonatal samples (blood samples and rectal swabs). The quantitative antibiogram method applied to 28 out of the 32 ESBL-producing strains revealed that the isolates were grouped into 5 clusters. Pulsed Field Gel Electrophoresis performed on a total of 16 ESBL-producing strains showed the existence of four profiles. A perfect concordance was observed between th

关键词： Resistance-Clone- Klebsiella pneumoniae -Pulsed Field Gel Electrophoresis-Quantitative Antibiogram

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Capacity Development of National Reference Centers (NRC) to Face the Challenges of Emerging Infections in Côte d’Ivoire

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Open Journal of Internal Medicine 2023年第3期13卷 181-208页

作者： Diané Kouao Maxime Adjogoua Edgard Valery Coulibaly Ngolo Antoine Marie-David Kadjo A. Hervé N’guessan Kouassi Raymond Sylla Yahaya Anne Blessa Jean Claude Beourou Sylvain guessennd-Kouadio Aya nathalie Dosso Mireille Tiembré Issiaka Pasteur Institute of Cô te d’Ivoire Abidjan C&ocircte d’Ivoire Biological Resource Center Abidjan C&ocircte d’Ivoire Epidemic Virus Department Abidjan C&ocircte d’Ivoire Molecular Biology Platform Abidjan C&ocircte d’Ivoire Mycobacterium Tuberculosis Unit Abidjan C&ocircte d’Ivoire Clinical Bacteriology Unit Abidjan C&ocircte d’Ivoire Department of Mycology Malariology Abidjan C&ocircte d’Ivoire Antibiotics Unit Abidjan C&ocircte d’Ivoire Department of Public Health UFR Medical Science Université Félix Houphou&eumlt-Boigny Abidjan C&ocircte d’Ivoire

Background: Some national and international strategies for the detection and prevention of emerging infectious diseases have been established across sectors. The capacity to carry out these tasks varies from country to country, and that remains largely undervalued. The Pasteur Institute of C?te d’Ivoire has created and implemented the capacity of national reference centers to fight against emerging and other infectious diseases. Objective: Show on the one hand the strategies used to develop the National Reference Centers and the IPCI and on the other hand the results obtained by performing these strategies. Method: Datas collection by documentary analysis (published scientific articles and grey literature) was done on Google Scholar, PUBMED and institutional reference documents. The documentary research was carried out to have a better understanding of strategies used to create and develop the NRCs in microbiology of communicable diseases. Results: Seven integrated strategies were launched: 1-training and workforce development;2-investigation of epidemics or public health events;3-strengthening laboratory epidemiological research;4-strengthening surveillance systems;5-improving communication with partners and stakeholders;6-building national and international collaborations;and 7-strengthening technical and technological platforms. In two decades, the number of researchers has risen from 10 in 2004 to ninety (90) in 2021, with 12 senior researchers and 32 junior researchers. A number of health service staff had attended a qualifying training course, 27 investigations into outbreaks and other public health events had been carried out, 18 short-term research projects had been launched, major surveillance programs and epidemiological research efforts on vector-borne, food-borne and nosocomial infections had begun, and several scientific manuscripts had been published or were edited in the writting press. Conclusion: The Ivorian experience shows that, with concerted eff

关键词： Evaluation Communicable Diseases National Reference Centerorlaboratorie Capacity Development Côte d’Ivoire

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