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Imidazolium-based ionic liquids as additives to preserve the Enhanced Green Fluorescent Protein fluorescent activity

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Green Chemical Engineering 2021年第4期2卷 412-422页

作者： nathalia v.veríssim. Carolina F.Saponi Timothy M.Ryan Tamar L.Greaves Jorge F.B.Pereira Department of Engineering of Bioprocesses and Biotechnology School of Pharmaceutical SciencesSao Paulo State University(UNESP)Rodovia Araraquara-Jaú/Km 0114800-903AraraquaraSPBrazil School of Science College of ScienceEngineering and HealthRMIT University124 La Trobe StreetMelbourneVIC 3000Australia School of Pharmaceutical Sciences Sao Paulo University(USP)Av.Prof.Lineu Prestesno.580B1605508-000Cidade de UniversitariaSao PauloSPBrazil Australian Synchrotron 800 Blackburn RoadClaytonVIC 3168Australia Univ Coimbra CIEPQPFDepartment of Chemical EngineeringRua Sílvio LimaPolo II–Pinhal de Marrocos3030-790 CoimbraPortugal

Fluorescent Proteins(FP)can be applied as biomarkers and biosensors in the industrial and medical fields,but their large-scale use,especially for new industrial applications,is limited due to their low ***,the discovery of additives capable of preserving the activity of FP at room temperature and under stress conditions can help to expand and facilitate their commercial *** this goal,we evaluated the application of 1-alkyl-3-methylimidazolium chloride-based([C_(n)mim]Cl)ionic liquids(ILs)as additives to preserve the activity of Enhanced Green Fluorescent Protein(EGFP)at different storage times and under unfavorable ***[C_(n)mim]Cl solutions(at 0.100 mol L^(-1))were able to preserve EGFP fluorescence for longer than the phosphatesaline buffer(PBS)and NaCl solutions,increasing its fluorescence manifestation from 1 to 3 months.[C_(n)mim]Cl with shorter to medium cationic alkyl chains were the most effective in preserving EGFP fluorescence.[C_(n)mim]Cl also protected EGFP activity in the presence of the surfactant SDS,the acid guanidine hydrochloride,and H_(2)O_(2).Therefore,[C_(n)mim]Cl can be added to aqueous solutions to preserve EGFP fluorescence activity at room temperature for longer storage times and to reduce the negative impact of denaturing agents on ***,there is a massive potential for the application of ILs as additives to preserve FP in the long-term without refrigeration and under unfavorable conditions,and this is fundamental to enable expansion of FP in industrial and commercial applications.

关键词： Green Fluorescent Protein Protein stability Fluorescence Ionic liquids Preservatives

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Effect of biochar on the metabolome of soybean seedlings

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Oil Crop Science 2024年第1期9卷 60-68页

作者： nathalia E.Silva Mariana G.Aguilar Osania E.Ferreira Gleicia M.Paulino Jaqueline C.L.Carvalho Lúcia P.S.Pimenta Alan R.T.Machado Programa de Pos-graduaçao em Ciencias Ambientais Universidade do Estado de Minas GeraisFrutalMinas Gerais38202-436Brazil Departamento de Química Universidade Federal de Minas GeraisMinas GeraisBelo Horizonte31270-901Brazil Departamento de Engenharia Aplicada e Tecnologias Ambientais Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil Departamento de Ciencias Exatas Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil

The use of biochar can have several effects on plant germination,depending on raw material,preparation method and application ***,the molecular mechanisms that lead to those results have yet to be *** aim of this research was to improve the understanding of these mechanisms by characterizing the metabolic effects of sugarcane bagasse biochar on soybean *** types of biochars were prepared by pyrolysis at 300℃(SCB300),400℃(SCB400)and 600℃(SCB600).Then,each one was mixed into sand at 1%,3%,5%(w/w)dose,*** experiment was performed in 8 days of incubation,when the number of germinated seeds and the average radicle length were *** evaluate the metabolome,the dry biomass(DB)was subjected to extraction with a mixture of methanol-d4 and D2O(1:1 v/v).The extracts were submitted to metabolomics analysis by Proton Nuclear Magnetic *** Relative Germination,Relative Average Radicle Growth and Germination Index increased in all treatments compared to *** the other hand,the DB increased in all treatments,except for SCB300,at doses of 1%and 3%w/*** metabolites(alanine,asparagine,acetic acid,citric acid,glycerol,fatty acids and sucrose)were identified and quantified in DB extracts as the most influential finding for the separation of *** together,these results strongly suggested that biochars accelerated the catabolism of triacylglycerols to sucrose and induced a slight osmotic stress.

关键词： Germination Metabolomics 1H NMR Pyrolysis Sugarcane bagasse

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Predicting major adverse cardiovascular events after orthotopic liver transplantation using a supervised machine learning model:A cohort study

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World Journal of Hepatology 2024年第2期16卷 193-210页

作者： Jonathan Soldera Leandro Luis Corso Matheus Machado Rech vinícius Remus Ballotin Lucas Goldmann Bigarella Fernanda Tomé nathalia Moraes Rafael Sartori Balbinot Santiago Rodriguez Ajacio Bandeira de Mello Brandão Bruno Hochhegger Post Graduate Program at Acute Medicine and Gastroenterology University of South WalesCardiff CF371DLUnited Kingdom Postgraduate Program in Pathology Federal University of Health Sciences of Porto Alegre(UFCSPA)Porto Alegre 90050-170Brazil Department of Engineering Universidade de Caxias do SulCaxias do Sul 95070-560Brazil School of Medicine Universidade de Caxias do SulCaxias do Sul 95070-560Brazil Postgraduate Program in Hepatology Federal University of Health Sciences of Porto Alegre(UFCSPA)Porto Alegre 90050-170Brazil

BACKGROUND Liver transplant(LT)patients have become older and *** rate of post-LT major adverse cardiovascular events(MACE)has increased,and this in turn raises 30-d post-LT *** cardiac stress testing loses accuracy when applied to pre-LT cirrhotic *** To assess the feasibility and accuracy of a machine learning model used to predict post-LT MACE in a regional *** This retrospective cohort study involved 575 LT patients from a Southern Brazilian academic *** developed a predictive model for post-LT MACE(defined as a composite outcome of stroke,new-onset heart failure,severe arrhythmia,and myocardial infarction)using the extreme gradient boosting(XGBoost)machine learning *** addressed missing data(below 20%)for relevant variables using the k-nearest neighbor imputation method,calculating the mean from the ten nearest neighbors for each *** modeling dataset included 83 features,encompassing patient and laboratory data,cirrhosis complications,and pre-LT cardiac *** performance was assessed using the area under the receiver operating characteristic curve(AUROC).We also employed Shapley additive explanations(SHAP)to interpret feature *** dataset was split into training(75%)and testing(25%)*** was evaluated using the Brier *** followed Transparent Reporting of a Multivariable Prediction Model for Individual Prognosis or Diagnosis guidelines for ***-learn and SHAP in Python 3 were used for all *** supplementary material includes code for model development and a user-friendly online MACE prediction *** Of the 537 included patients,23(4.46%)developed in-hospital MACE,with a mean age at transplantation of 52.9 *** majority,66.1%,were *** XGBoost model achieved an impressive AUROC of 0.89 during the training *** model exhibited accuracy,precision,recall,and F1-score values of 0.84,0.85,0.80,and 0.79,***

关键词： Liver transplantation Major adverse cardiac events Machine learning Myocardial perfusion imaging Stress test

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Personalized medicine:Clinical oncology on molecular view of treatment

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World Journal of Clinical Oncology 2024年第8期15卷 992-1001页

作者： Rafick Costa Dos Santos Da Silva nathalia de Andrade Simon AndréAlves Dos Santos Gabriel De Melo Olegário Jayne Ferreira Da Silva Naide Oliveira Sousa Manuel Alvarez Troncoso Corbacho Fabrício Freire de Melo Instituto Multidisciplinar em Saúde Universidade Federal da BahiaVitória Da Conquista 45029-094BahiaBrazil

Cancer,the second leading global cause of death,impacts both physically and *** treatments such as surgeries,chemotherapy,and radiotherapy have adverse effects,driving the need for more precise *** medicine enables more targeted *** mapping,alongside other molecular biology approaches,identifies specific genes,contributing to accurate *** review addresses,in clinical use,a molecular perspective on *** like alpha-fetoprotein,beta-human chorionic gonadotropin,5-hydroxyindoleacetic acid,programmed death-1,and cytotoxic T lymphocyte-associated protein 4 are explored,providing valuable ***,with an emphasis on artificial intelligence,revolutionizes the analysis of biological data,offering more accurate *** like liquid biopsy are emphasized for early *** medicine guides therapeutic strategies based on the molecular characteristics of the tumor,as evidenced in the molecular subtypes of breast *** allow personalized treatments,highlighting the role of trastuzumab and endocrine *** the benefits,challenges persist,including high costs,tumor heterogeneity,and ethical *** obstacles requires collaboration,ensuring that advances in molecular biology translate into accessible benefits for all.

关键词： Oncology Neoplasia Molecular biology Personalized medicine Molecular Markers Cancer

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Prey and predators perceive orb-web spider conspicuousness differently: evaluating alternative hypotheses for color polymorphism evolution

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Current Zoology 2019年第5期65卷 559-570页

作者： nathalia G.XIMENES Felipe M.GAWRYSZEWSKI Animal Colouration and Evolution Lab Departamento de ZoologiaInstituto de Ciencias BiologicasUniversidade de BrasiliaBrasiliaDF70910-900Brazil Pos-Graduaqao em Ecologia e Evoluqao Universidade Federal de GoiasCampus SamambaiaGoianiaGO74690-900Brazil

Color polymorphisms have been traditionally attributed to apostatic *** perception of color depends on the visual system of the *** models predict that differently perceived degrees of conspicuousness by two predator and prey species may cause the evolution of polymorphisms in the presence of anti-apostatic and apostatic *** spider Gasteracantha cancriformis(Araneidae)possesses several conspicuous color *** orb-web spiders,the prey attraction hypothesis states that conspicuous colors are prey lures that increase spider foraging success via flower ***,polymorphism could be maintained if each morph attracted a different prey species(multiple prey hypothesis)and each spider mimicked a different flower color(flower mimicry hypothesis).Conspicuous colors could be a warning signal to predators because of the spider's hard abdomen and *** predators could perceive morphs differently and exert different degrees of selective pressures(multiple predator hypothesis).We explored these 3 hypotheses using reflectance data and color vision modeling to estimate the chromatic and achromatic contrast of *** morphs as perceived by several potential prey and predator *** results revealed that individual taxa perceive the conspicuousness of morphs ***,the multiple prey hypothesis and,in part,the multiple predator hypothesis may explain the evolution of color polymorphism in ***,even in the presence of anti-apostatic *** flower mimicry hypothesis received support by color metrics,but not by color vision *** parameters not evaluated by color vision models could also affect the perception of morphs and influence morph survival and polymorphism stability.

关键词： aposematism Araneidae Gasteracantha cancriformis luring prey attraction visual communication

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Conflicting Discourses on Wildfire Risk and the Role of Local Media in the Amazonian and Temperate Forests

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International Journal of Disaster Risk Science 2019年第4期10卷 529-543页

作者： nathalia Thais Cosmo da Silva Urbano FraPaleo Jose Ambrosio Ferreira Neto Departamento de Economia Rural Universidade Federal de VicosaCampus UniversitarioEdificio Edson Potsch MagalhaesVicosaMG 36570-900Brazil School of Social Sciences and Humanities University of ExtremaduraCampus of Caceres City10071 CaceresSpain

This article examines how risk is communicated by different actors,particularly local print newspapers and actors at the community level,in two different geographical contexts that are severely affected by wildfires—the Brazilian Amazon and Atlantic *** analyzed how wildfire risk is framed in local print media and local actor discourse to elucidate how wildfire risk is interpreted and aimed to identify the main priorities of these risk governance *** main findings reveal that the presentation of wildfire as a spectacle is a serious obstacle to the promotion of coherent risk governance and social learning,which involves recognizing wildfire risk as a social,political,economic,and environmental *** risk governance should communicate the multifaceted nature of risk and stimulate dialogue and negotiation among all actors to build consensus regarding land use and the creation of risk.

关键词： Discourse analysis Framing Galicia Local media Risk communication Rondonia Wildfire risk

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Effects of Imazethapyr-Based Herbicide Formulation in the Zebrafish (Danio rerio) Hepatocyte Cell Line (ZF-L): Cytotoxicity and Oxidative Stress

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Journal of Environmental Protection 2024年第6期15卷 747-765页

作者： Edila Maria Kickhöfel Ferrer Milena Mattes Cerveira Pedro Henrique Flores Da Cruz Cleiton Jesus Andrade Pereira Lucas Moraes Berneira Claudio Martin Pereira de Pereira nathalia Stark Pedra Roselia Maria Spanevello Jean Carlos Bauer vieira Clarissa Piccinin Frizzo Mateus Tavares Kütter Janice Luehring Giongo Rodrigo de Almeida vaucher Center of Chemical Pharmaceutical and Food Sciences Microorganism Biochemistry and Molecular Biology Research Laboratory (LAPEBBIOM) Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Laboratory for Lipidomic and Bio-Organic Research Bioforensic Research Group Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Center of Chemical Pharmaceutical and Food Sciences Neurochemistry Inflammation and Cancer Laboratory Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil Department of Chemistry Heterocycle Chemistry Center (NUQUIMHE) Federal University of Santa Maria (UFSM) Santa Maria Rio Grande do Sul Brazil Department of Microbiology and Parasitology Biology Institute Federal University of Pelotas (UFPEL) Pelotas Rio Grande do Sul Brazil

Seizures of agrochemical formulations have increased in Brazil and Rio Grande do Sul is among the Brazilian states with the highest number of seizures of these products obtained illicitly. The use of illicit formulations can cause significant harm to agricultural production, the environment, and non-target species. This study evaluated the cytotoxicity and oxidative stress of a seized formulation containing the herbicide imazethapyr (IMZT). Characterization of the herbicide included gas chromatography-mass spectrometry (GC-MS) and thermal analyses (thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC)). Hemolytic and cytotoxicity assays in ZF-L hepatic cells showed IC50 values of 12.75 µg/mL, 3.01 µg/mL, 2.67 µg/mL, and 1.61 µg/mL for erythrocytes, [3(4,5-dimethyl)-2 bromide-5 diphenyl tetrazolium] (MTT), neutral red (NR), and lactate dehydrogenase (LDH) assays, respectively. The median IC50 of 2.84 µg/mL was used in oxidative stress assays, revealing increased reactive oxygen species (ROS) production, reduced total sulfhydryl content, and decreased superoxide dismutase (SOD) and catalase (CAT) activity. This study is the first to report in vitro oxidative stress induced by IMZT in the ZF-L cell line, emphasizing the importance of in vitro assays for assessing the toxic effects of seized agrochemicals on human health and the environment.

关键词： Agrochemical Imidazolinone Zebrafish Cell viability Oxidative Damage

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Assessment of palmar subcutaneous tissue vascularization in patients with Dupuytren's contracture

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World Journal of Orthopedics 2018年第9期9卷 130-137页

作者： nathalia Shchudlo Tatyana varsegova Tatyana Stupina Tamara Dolganova Michael Shchudlo nathalia Shihaleva vadim Kostin Clinics and Experimental Laboratory for Reconstructive Microsurgery and Hand Surgery Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics" Laboratory of Morphology of Federal State Budget Institution Russian Ilizarov Scientific Center "Restorative Traumatology and Orthopaedics"

AIM To investigate the structural and functional characte-ristics of palmar hypodermal tissue vascularization in Dupuytren's contracture patients of different *** Eighty-seven Dupuytren's contracture patients underwent partial fasciectomy. Twenty-two of them were less than 55 years old(Y-group, n = 22); the others were 55 and older(O-group, n = 65). In surgically excised representative tissue samples, a histomorphometric analysis of the perforating arteries of the palmar aponeurosis and stereologic analysis of hypodermis vascularity were performed. The method of laser flowmetry estimated the microcirculation of the skin of the *** Frequency of cases with rapid development of contracture(less than 5 years) was 13.6% in the Y-group and 40% in the O-group, P verely in Y. The thickness of intima increased three times compared with healthy control, and the intima/media relation also increased, especially in O. Increased numerical and volumetric micro-vessel densities in hypodermis, percentage of large vessels(more than 12 μm in diameter), and percentage of vessels with signs of periadventitial inflammatory infiltration were noted in Y. The percentage of vessels with adventitial fibrosis was greater in O than in Y. Base capillary flow in Y was increased compared to healthy control subjects and to O, and peak capillary flow was increased in comparison with *** Compared to the O-group, Y-group patients exhibited more severe constrictive remodeling of palmar fascia perforating arteries supplying hypodermis but more expressed compensatory changes of its capillarization.

关键词： Dupuytren’s contracture Laser Doppler flowmetry Hypodermis Histo-morphometry Palmar fascia

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Effect of mini-cutting size on adventitious rooting and morphophysiological quality of Ilex paraguariensis plantlets

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Journal of Forestry Research 2021年第2期32卷 815-822页

作者： nathalia Pimentel Denise Gazzana Janaina de Fatima Spanevello Kelen Haygert Lencina Dilson Antônio Bisognin Programa de Pós-Graduação em Engenharia Florestal Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil Curso de Graduação em Agronomia Centro de Ciências Rurais da Universidade Federal de Santa MariaAvenida Roraima1000Santa MariaRS 97105-900Brazil Departamento de Fitotecnia Centro de Ciências RuraisUniversidade Federal de Santa MariaAv.Roraima1000Santa MariaRS 97105-900Brazil

As the energizing and therapeutic effects of infusions of mate(Ilex paraguariensis ***.-Hil)leaves are increasingly recognized,rising global consumption is also increasing the demand for plantlets with high genetic and morphological ***-cuttings are an alternative for clonal plantlets;however,more details are needed on factors that affect the success of this ***,toward maximizing production,we evaluated adventitious rooting competence of mini-cuttings of different sizes and the morphophysiological quality of the resultant ***(one bud;length up to 2.5 cm),medium(multiple buds;length 2.6–5.0 cm),and large(multiple buds;length 5.1–10.0 cm)mini-cuttings were planted in a mixture of commercial substrate,vermiculite,and coarse sand(1:1:1 v:v:v),grown in a humidity chamber,then evaluated after 45,60,75,and 90 days for survival,percentage of callus and shoot formation,number and length of shoots,percentage of rooted cuttings,and number and length of *** rooted mini-cuttings were then cultivated in commercial substrate and subsurface soil(2:1 v:v)and evaluated after 30,60,90 and 120 days of cultivation for morphophysiology of the plantlets,percentage of survival,number of leaves,shoot height,stem diameter,and ratio of shoot height to stem *** 120 days,the plantlets were evaluated for the ratio of shoot and root dry mass,total length,surface area,total volume,and mean diameter of roots and Dickson quality *** rooting of the mini-cuttings and the morphophysiological quality of the plantlets were not influenced by the size of the vegetative ***,single-bud mini-cuttings have similar adventitious rooting competence,and result in plantlets with adequate morphophysiological quality,based upon leaf development,shoot height,stem diameter and length,and surface area,volume and diameter of ***-bud mini-cuttings up to 2.5 cm long can thus be used to maximize production of mate plantlets by mini-c

关键词： Aquifoliaceae Clonal forestry Mini-cutting technique Plantlet production vegetative propagation

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Optimization of an innovative approach involving mechanical activation and acid digestion for the extraction of lithium from lepidolite

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International Journal of Minerals,Metallurgy and Materials 2018年第1期25卷 11-19页

作者： nathalia vieceli Carlos A.Nogueira Manuel F.C.Pereira Fernando O.Durao Carlos Guimaraes Fernanda Margarido Center for Innovation Technology and Po icy Research-IN+Instituto Superior T6cnicoUniversity of Lisbon1049-001 LisboaPortugal LNEG-Laborat6rio Nacional de Energia e Geologia I.P.Campus do Lumiar1649-038 LisboaPortugal CERENA-Centro de Recursos Naturais e Ambiente DECivilInstituto Superior T6cnicoUniversity of Lisbon1049-001 LisboaPortugal

The recovery of lithium from hard rock minerals has received increased attention given the high demand for this element. There- fore, this study optimized an innovative process, which does not require a high-temperature calcination step, for lithium extraction from le- pidolite. Mechanical activation and acid digestion were suggested as crucial process parameters, and experimental design and re- sponse-surface methodology were applied to model and optimize the proposed lithium extraction process. The promoting effect of amorphi- zation and the formation of lithium sulfate hydrate on lithium extraction yield were assessed. Several factor combinations led to extraction yields that exceeded 90%, indicating that the proposed process is an effective approach for lithium recovery.

关键词： lepidolite lithium mechanical activation acid digestion optimization extraction

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