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Mechanobiology and Cartilage Tissue Engineering

生物医学工程学杂志 2005年第S1期22卷 33-34页

作者： Céline HUSELSTEIN natalia de isla Jing-Wei YANG Yun WANG Sylvaine MULLER Jean-Fran·ois STOLTZ Research Group of Cell and Tissue Engineering LEMTA-CNRS UMR 7563 et IFR 111 BioingénierieFacultéde Médecine54505 Vandoeuvre-lès-Nancy CedexFrance Unitéde Thérapie Cellulaire et Tissulaire(UTCT)-CHRU-54500 Vandoeuvre-lès-Nancy Cedex France

1 Introduction The cartilage i s a hydrated connective tissue in joint s that withstands and distributes mechanical *** utilize mechanical signals to maintain tissue *** regulate their metabolic activity through complex biological and biophysical interactions with the extracellular matrix(ECM).Although some of the mechanisms of mechanotransduction are known today,there are certainly many others left *** topics of chondrocytes mechanobiology have led to the development of tissue *** is the concept of substitute tissue developed in vitro,from bioresorbable or non2bioresorbable scaffolds and from cells harvested in a physiologic mechanical environment.

关键词： Ca MSCs Mechanobiology and Cartilage Tissue Engineering

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Validation of an In Vitro Model to Study Human Cartilage Responses to Compression

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Engineering（科研） 2012年第10期4卷 61-64页

作者： natalia de isla Céline Huselstein Didier Mainard Jean-Francois Stoltz CNRS UMR 7561 BiopoleFaculte de MédecineUniversite de LorraineVandoeuvreFrance UTCT CHU de NancyVandoeuvreFrance COT CHU de NancyVandoeuvreFrance

The aim of this work was to develop an in vitro model to study mechanical compression effects on cartilage. A pressure-controlled compression device was used in this study. Cartilage explants obtained from human knee were compressed at 1MPa/1Hz for 7 hours (30 min ON, 30 min OFF) under normoxia (5% CO2, 21% O2) or hypoxia (5% CO2, 5% O2). Cell viability was analyzed while nitric oxide (NO) and glycosaminoglycans (GAG) release was assayed in culture media. Mechanical stimulation increased NO production and GAG release by human cartilage explants under normoxia and hypoxia culture. In normoxia and hypoxia conditions, mechanical stimulation alters human OA cartilage metabolism. There is also, an increase in matrix degradation after compression, as shown by levels of GAG found in culture media. This study put in evidence the importance of mechanical compression in the progression of the osteoarthritis and present and in vitro model for mechanobiological and pharmacological studies.

关键词： Cartilage Compression In Vitro Model Nitric Oxide GAG

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Non-Invasive Second Harmonic Generation (SHG) in Macroscopy (MacroSHG) as Bio-Diagnosis to Image Collagen Network Organization in Extracellular Matrix

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Engineering（科研） 2014年第8期6卷 485-490页

作者： Dominique Dumas Elisabeth Werkmeister Sebastien Hupont Céline Huselstein natalia de isla Marthe Rousseau Patrick Menu Didier Mainard Ingénierie Moléculaire et Thérapeutique (CNRS FR 3209) Plate Forme d’Imagerie Cellulaire et Tissulaire PTIBC-IBISA NANCY Nancy France Plate-Forme Microscopie-Imagerie-Cytométrie du campus Pasteur Lille (MICPaL) IFR 142 Institut Pasteur de Lille Institut de Biologie de Lille Lille France Universite de Lorraine Physiopathologie Pharmacologie et Ingénierie Articulaires (IMOPA CNRS 7365) Nancy France Universite de Lorraine Physiopathologie Pharmacologie et Ingénierie Articulaires (IMOPA CNRS 7365) Nancy France Ingénierie Moléculaire et Thérapeutique (CNRS FR 3209) Plate Forme d’Imagerie Cellulaire et Tissulaire PTIBC-IBISA NANCY Nancy France

In this work, we have considered a new multimodality imaging for macroscopy based on Second Harmonic Generation (SHG) method to monitor invasivelessly the matrix collagen. As the triple helicoidally structure of collagen molecules appearing as not centrosymetric, very organized and spatially oriented, collagen fibrils give rise to a very strong SHG signal and can be imaged without any exogenous dye. To integrate a multidimensional scale with a large field of view (non-sliced samples), we have adapted and validated an instrumental coupling between a two photon excitation laser and a macroscope to collect cartography of SHG signal. We introduced an index (F-SHG) based on decay time response measured by TCSPC for respectively Fluorescence (F) and Second Harmonic Generation (SHG) values. For various sample where protein collagen is the major component of extracellular matrix (vessel, skin, carotide vessel, rat femoral head cartilage, mouse tumor, human wharton’s jelly and rat tendon) or not (nacre), we compared the index distribution obtained with MacroSHG. In this work, we showed for the first time that multiscale large field imaging (Macroscopy) combined to Multimodality approaches (SHG-TCSPC) could be an innovative and non-invasive technique to detect and identify some biological interest molecules (collagen) in biomedical topics.

关键词： Multiphoton Macroscopy Second Harmonic Generation Collagen Extracellular Matrix

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Does Making Method of Alginate Hydrogel Influence the Chondrogenic Differentiation of Human Mesenchymal Stem Cells?

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Engineering（科研） 2012年第10期4卷 110-113页

作者： Jessica Schiavi Naceur Charif natalia de isla Daniele Bensoussan Jean-Francois Stoltz Nadia Benkirane-Jessel Celine Huselstein INSERM-UMR Faculty of medicineStrasbourg UniversityStrasbourgFrance Universitede Lorraine CNRS-UMR–Campus Sante–BiopoleFaculty of medicineLorraine UniversityVandouvre-les-NancyFrance

To overcome cartilage injury, strategies have been developed in the last few years based on tissue engineering to rebuild the defects. Cartilage engineering is principally based on three main biological factors: cells (native cells (chondrocytes) or a more primitive ones as mesenchymal stem cells), scaffolds and functionalization factors (growth factors, mechanical stimulation and/or hypoxia). Cartilage tissue engineering strategies generally result in homogeneous tissue structures with little resemblance to native zonal organization of articular cartilage. The main objective of our work concerns the buildup of complex biomaterials aimed at reconstructing biological tissue with three dimensional cells construction for mimicking cartilage architecture. Our strategy is based on structures formation by simple and progressive spraying of mixed alginate hydrogel and human mesenchymal stem cells (hMSC). In this work, the comportment of cells and more precisely their chondrogenic differentiation potential is compared to a traditional making process: the mold. We report here that spraying method allowed to product a scaffold with hMSC that confer a favorable environment for neocartilage construction.

关键词： Component: Cartilage Tissue Engineering Sodium Alginate Sprayed method Molded Method

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Russian Collaborative development of Reproduction Technologies for the Sustainable Management of Amphibian Biodiversity

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Asian Herpetological Research 2023年第1期14卷 103-115页

作者： Victor K.UTESHEV Edith N.GAKHOVA Ludmila I.KRAMAROVA natalia V.SHISHOVA Svetlana A.KAUROVA Elena A.KIDOVA Artem A.KIDOV Robert K.BROWNE Institute of Cell Biophysics Russian Academy of SciencesPushchinoMoscow 142290Russia Institute of Theoretical and Experimental Biophysics Russian Academy of SciencesPushchinoMoscow 142290Russia Russian State Agrarian University-K.A.Timiryazev Agricultural Academy Moscow 127550Russia Sustainability America La Isla RoadCorozal DistrictBelize

Reproduction technologies(RTs)can provide for the reliable reproduction of amphibians,as well as perpetuation of species genetic variation with the use of *** 1982,in anticipation of the biodiversity conservation crisis,major Russian institutions collaborated in a dynamic program to develop and implement RTs for the sustainable management of amphibian *** initial primary focus was the captive breeding of threatened Russian endemic anuran and caudate species,using RTs that varied from environmental manipulation to the use of exogenous gonadotropic hormones to stimulate *** species were mostly from Palearctic or cool mountain regions,but also included a wide range of species from warm *** early achievements included the successful cryopreservation of anuran spermatozoa and anuran diploid pluripotent cell nuclei,in order to store both the matrilineal and patrilineal genomes in biobanks,with their subsequent development to the blastula stage after implantation into enucleated *** the turn of the 21st Century,in support of the priorities of the Amphibian Conservation Action Plan(2007),we developed RTs for the refrigerated storage of testicular or urinary spermatozoa for days to weeks at 4℃,the cryopreservation of urinary spermatozoa using anovel cryoprotectant,the in vitro fertilisation of hormonally induced oocytes either fresh or after refrigerated ex situ or in situ storage,and the artificial insemination of salamanders with fresh *** this article,we describe previously unpublished techniques and techniques from obscure Russian sources.

关键词： artificial fertilisation assisted reproductive technologies(ART) cryopreservation gonadotropic hormones oocytes spermatozoa storage urinary spermatozoa

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