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Xyloglucan for Generating Tensile Stress to Bend Tree Stem

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Molecular Plant 2009年第5期2卷 893-903页

作者： Kei'ichi Baba Yong Woo Park Tomomi Kaku Rumi Kaida Miyuki Takeuchi Masato Yoshida Yoshihiro Hosoo Yasuhisa Ojio Takashi Okuyama Toru Taniguchi Yasunori Ohmiya Teiji Kondo Ziv Shani Oded Shoseyov Tatsuya Awano Satoshi Serada Naoko Norioka Shigemi norioka Takahisa Hayashi Kyoto University RISH Gokasho Uji Kyoto 611-0011 Japan Graduate School of Bioagricultural-Sciences Nagoya University Nagoya 464-8601 Japan Forest Tree Breeding Center Hitachi Ibaraki 319-1301 Japan CBD Technologies Rehovot 76100 Israel Division of Forest and Biomaterials Science Kyoto University Kyoto 606-8502 Japan Graduate School of Frontier Biosciences Osaka University Suita Osaka 565-0871 Japan Institute of Sustainability Science Kyoto University Gokasho Uji Kyoto 611-0011 Japan

In response to environmental variation, angiosperm trees bend their stems by forming tension wood, which consists of a cellulose-rich G （gelatinous）-Iayer in the walls of fiber cells and generates abnormal tensile stress in the secondary xylem. We produced transgenic poplar plants overexpressing several endoglycanases to reduce each specific polysaccharide in the cell wall, as the secondary xylem consists of primary and secondary wall layers. When placed horizontally, the basal regions of stems of transgenic poplars overexpressing xyloglucanase alone could not bend upward due to low strain in the tension side of the xylem. In the wild-type plants, xyloglucan was found in the inner surface of G-layers during multiple layering. In situ xyloglucan endotransglucosylase （XET） activity showed that the incorporation of whole xyloglucan, potentially for wall tightening, began at the inner surface layers S1 and S2 and was retained throughout G-layer development, while the incorporation of xyloglucan heptasaccharide （XXXG） for wall loosening occurred in the primary wall of the expanding zone. We propose that the xyloglucan network is reinforced by XET to form a further connection between wall-bound and secreted xyloglucans in order to withstand the tensile stress created within the cellulose G-layer micro fibrils.

关键词： G-layer tensile stress xyloglucan xyloglucan endotransglucosylase

日本梨树(Pyrus Pyrifolia)花粉似β-1,3-葡聚糖酶的蛋白质的cDNA克隆和序列分析

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日本梨树(Pyrus Pyrifolia)花粉似β-1,3-葡聚糖酶的蛋白质的cDNA...

Potential utility of aldose reductase-deficient Schwann cells IKARS1 for the study of axonal degeneration and regeneration

中国生物化学与分子生物学会第八届会员代表大会暨全国学术...

作者：周跃钢 Naoko Norioka 李绍良 Shigemi norioka 西南农业大学生物化学教研组日本大阪大学蛋白质研究所

在配子体自交不亲和性机理的研究中,一个日本梨树(Pyrus pyrifolia)花粉中专一表达的蛋白质被发现.编码这一蛋白质(BGN-1)的cDNA(bgn-1)被克隆和测序.Bgn-1含有一个47bp的5’端非翻译区,一个1194bp的ORF和一个167bp的3’端非翻译区;一个...

在配子体自交不亲和性机理的研究中,一个日本梨树(Pyrus pyrifolia)花粉中专一表达的蛋白质被发现.编码这一蛋白质(BGN-1)的cDNA(bgn-1)被克隆和测序.Bgn-1含有一个47bp的5’端非翻译区,一个1194bp的ORF和一个167bp的3’端非翻译区;一个NUE(AATTAA)位于位点1369-1374.BGN-1包含了一个信号肽(22aa)和一个成熟蛋白质(375aa).成熟蛋白质的理论分子量和pI值分别是40723.95和9.59,并在C端延

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Neural Regeneration Research 2018年第6期13卷 979-980页

作者： naoko Niimi Kazunori Sango Diabetic Neuropathy Project Department of Sensory and Motor Systems Tokyo Metropolitan Institute of Medical Science

Diabetic peripheral neuropathy（DPN）is one of the most common and intractable complications of diabetes mellitus.Its irritating symptoms,such as paresthesia,hyperalgesia and allodynia,can be causes of insomnia and depression;whereas its progression to more advanced stages can result in serious consequences,such as lower limb amputations and lethal arrhythmias.

关键词： DPN Potential utility of aldose reductase-deficient Schwann cells IKARS1 for the study of axonal degeneration and regeneration NGF NCV AR PNS

Role of stereotactic body radiation therapy for hepatocellular carcinoma

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World Journal of Gastroenterology 2014年第12期20卷 3100-3111页

作者： naoko Sanuki Atsuya Takeda Etsuo Kunieda Radiation Oncology Center Ofuna Chuo Hospital Department of Radiation Oncology Tokai University

The integration of new technologies has raised an interest in liver tumor radiotherapy,with literature evolving to support its efficacy.These advances,particularly stereotactic body radiation therapy(SBRT),have been critical in improving local control or potential cure in liver lesions not amenable to first-line surgical resection or radiofrequency ablation.Active investigation of SBRT,particularly for hepatocellular carcinoma(HCC),has recently started,yielding promising local control rates.In addition,data suggest a possibility that SBRT can be an alternative option for HCC unfit for other local therapies.However,information on optimal treatment indications,doses,and methods remains limited.In HCC,significant differences in patient characteristics and treatment availability exist by country.In addition,the prognosis of HCC is greatly influenced by underlying liver dysfunction and treatment itself in addition to tumor stage.Since they are closely linked to treatment approach,it is important to understand these differences in interpreting outcomes from various reports.Further studies are required to validate and maximize the efficacy of SBRT by a large,multi-institutional setting.

关键词： Hepatocellular carcinoma Liver cirrhosis Liver neo

Degradation rates and products of fluticasone propionate in alkaline solutions

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Journal of Pharmaceutical Analysis 2017年第5期7卷 297-302页

作者： Tadakazu Tokumura naoko Yoshida Kanami Mori-Yasumoto Osamu Shirota Takuro Kurita Laboratory of Pharmaceutics Kagawa School of Pharmaceutical Sciences Tokushima Bunri University Department of Pharmaceutical Sciences School of Pharmacy International University of Health and Welfare Laboratory of Pharmacognosy and Natural Products Chemistry Kagawa School of Pharmaceutical Sciences Tokushima Bunri University

The apparent degradation rate constant of fluticasone propionate(FLT) in 0.1 M NaOH:methanol=1:1 at 37 °C was previously reported to be 0.169 ± 0.003 h^(-1), and four degradation products(products 1–4) were observed in the solution. The aims of the present study were to assess the degradation rates of FLT in other alkaline solutions and clarify the chemical structures of the four degradation products in order to obtain basic data for designing an enema for inflammatory bowel disease. The apparent degradation rate constants in 0.05 M NaOH and 0.1 M NaOH:CH_3CN=1:1 were 0.472 ± 0.013 h^(-1) and 0.154 ± 0.000 h^(-1)(n=3), respectively. The chemical structures of products 1–4 in 0.1 M NaOH:methanol=1:1 were revealed by nuclear magnetic resonance(NMR)and mass spectrometry data. The chemical structure of products 2 was that the 17-position of the thioester moiety of FLT was substituted by a carboxylic acid. The degradation product in 0.1 M NaOH:CH_3CN=1:1 was found to be product 2 based on ~1H NMR data. The degradation product in 0.05 M NaOH was considered to be product 2 based on the retention time of HPLC. These results are useful for detecting the degradation products of FLT by enzymes of the intestinal bacterial flora in the large intestine after dosing FLT as an enema.

关键词： Fluticasone propionate Degradation rate Degradation products Chemical structure Alkaline solution

Visual localization for asteroid touchdown operation based on local image features

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Astrodynamics 2020年第2期4卷 149-161页

作者： Yoshiyuki Anzai Takehisa Yairi Naoya Takeishi Yuichi Tsuda naoko Ogawa The University of Tokyo Tokyo113-8654Japan RIKEN Center for Advanced Intelligence Project Tokyo103-0027Japan Japan Aerospace Exploration Agency Sagamihara252-5210Japan

In an asteroid sample-return mission,accurate position estimation of the spacecraft relative to the asteroid is essential for landing at the target point.During the missions of Hayabusa and Hayabusa2,the main part of the visual position estimation procedure was performed by human operators on the Earth based on a sequence of asteroid images acquired and sent by the spacecraft.Although this approach is still adopted in critical space missions,there is an increasing demand for automated visual position estimation,so that the time and cost of human intervention may be reduced.In this paper,we propose a method for estimating the relative position of the spacecraft and asteroid during the descent phase for touchdown from an image sequence using state-of-the-art techniques of image processing,feature extraction,and structure from motion.We apply this method to real Ryugu images that were taken by Hayabusa2 from altitudes of 20 km-500 m.It is demonstrated that the method has practical relevance for altitudes within the range of 5-1 km.This result indicates that our method could improve the efficiency of the ground operation in the global mapping and navigation during the touchdown sequence,whereas full automation and autonomous on-board estimation are beyond the scope of this study.Furthermore,we discuss the challenges of developing a completely automatic position estimation framework.

关键词： visual navigation structure from motion asteroid touchdown Hayabusa2

Characterization of Pyrolytic Lignin Extracted from Bio-oil

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Chinese Journal of Chemical Engineering 2010年第6期18卷 1018-1022页

作者： JIANG Xiaoxiang naoko Ellis ZHONG Zhaoping Thermoenergy Engineering Research Institute Southeast University Nanjing 210096 China Department of Chemical and Biological Engineering University of British Columbia Vancouver Canada

Bio-oil is a new liquid fuel produced by fast pyrolysis,which is a promising technology to convert bio-mass into liquid. Pyrolytic lignin extracted from bio-oil,a fine powder,contributes to the instability of bio-oil. The paper presents the structural features of three kinds of pyrolytic lignin extracted from bio-oil with different methods(WIF,HMM,and LMM) . The pyrolytic lignin samples are characterized by Fourier transform infrared spectrometer(FTIR) and X-ray photoelectron spectroscopy(XPS) . FTIR data indicate that the three pyrolytic lignin samples have similar functional groups,while the absorption intensity is different,and show characteristic vibra-tions of typical lignocellulosic material groups O H(3340-3380 cm-1) ,C H(2912-2929 cm-1) and C O(1652-1725 cm-1) . Comparison in the region(3340-3380 cm-1) indicates that WIF has more O H stretch groups than HMM and LMM. The carbon spectra are fitted to four peaks:C1,C C or C H,BE 283.5 eV;C2,C OR or C OH,BE 284.5-285.8 eV;C3,C O or HO C OR,BE 286.10-287.10 eV;C4,O C O,BE 287.5-287.7 eV. The absence of C1,C C or C H indicates the dominant polymerization structure of aro-matic carbon in pyrolytic lignin samples. For HMM and WIF,C2a and C2b can not be separated,so there is no free hydroxyl group in the samples. The oxygen peaks are also fitted to four peaks:O1,OH,BE = 530.3 eV;O2,RC O,BE 531.45-531.72 eV;O3,O C O,BE = 532.73-533.74 eV;O4,H2O,BE 535 eV. The absence of O1 and O4 indicates that little hydroxyl groups and adsorbed water are present in the samples.

关键词：快速热解生物油木质素 X射线光电子能谱傅立叶变换红外光谱仪提取表征影响因子

EXILE SHOKICHI in ViVi 男性和女性间性吸引力的话题

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美眉 2019年第6期 130-135页

作者： Melon naoko Yamamoto

距离上一张专辑发行后的第1114天——。粉丝们一直在期待的EXILE SHOKICHI第二张个人专辑已于今年5月发行。这张专辑不仅仅显示了他乐感的增长,还让人强烈地感受到一个成熟男人倍增的性感吸引力。在专辑制作结束后,实现了与瑛茉Jasmine... 详细信息

距离上一张专辑发行后的第1114天——。粉丝们一直在期待的EXILE SHOKICHI第二张个人专辑已于今年5月发行。这张专辑不仅仅显示了他乐感的增长,还让人强烈地感受到一个成熟男人倍增的性感吸引力。在专辑制作结束后,实现了与瑛茉Jasmine极具"色香味"的特别拍摄。那股色彩的冲击力,以及即将开始的他的solo活动,让人心中充满了期待。

关键词：个人专辑 EXILE SHOKICHI in ViVi 性吸引力 MV

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生物油/生物柴油混合燃料的热稳定性

东南大学学报（自然科学版） 2011年第1期41卷 129-133页

作者：仲兆平姜小祥 naoko Ellis 东南大学能源与环境学院南京210096 加拿大英属哥伦比亚大学生物与化学工程系

为了考察生物油/生物柴油混合燃料的热稳定性,分析了该混合燃料在不同的存储温度和存储时间下组分物化性质的变化,并测试了存储前后该混合燃料的黏度、水分含量、酸度、平均分子质量等理化特性.实验结果表明:当存储温度为80℃,存储时间... 详细信息

为了考察生物油/生物柴油混合燃料的热稳定性,分析了该混合燃料在不同的存储温度和存储时间下组分物化性质的变化,并测试了存储前后该混合燃料的黏度、水分含量、酸度、平均分子质量等理化特性.实验结果表明:当存储温度为80℃,存储时间为180 h时,样品的含水量小于0.7%;黏度随存储温度的升高和存储时间的延长变化不大.利用FTIR和GPC分析了样品在存储过程中的化学变化.GPC结果表明,样品的分子质量有所增大,原因可能是在存储过程中发生了聚合或缩合反应.FTIR结果表明,生物油/生物柴油混合燃料中存在O—H伸缩振动、C—H伸缩振动和C=O伸缩振动,这些官能团吸收强度的增加说明在存储过程中发生了酯化、醚化等化学反应.

关键词：生物油生物柴油老化存储黏度酸度