检索结果-南通市图书馆

Ligand density-dependent influence of arginine-glycine- aspartate functionalized gold nanoparticles on osteogenic and adipogenic differentiation of mesenchymal stem cells

在线全文

学校读者我要写书评

暂无评论

Nano Research 2018年第3期11卷 1247-1261页

作者： Jingchao Li Ying Chen naoki Kawazoe Guoping Chen Intemational Center for Materials Nanoarchitectonics National lnstitute for Materials Science 1-1 Namiki Tsukuba Ibaraki 305-0044Japan Department of Materials Science and Engineering Graduate School of Pure and Applied Sciences University of Tsukuba 1-1-1 Tennodai Tsukuba Ibaraki 305-8577 Japan Research Center for Functional Materials National Institute for Materials Science 1- 1 Namiki Tsukuba Ibaraki 305-0044 Japan

Extracellular matrix （ECM） plays a very important role in regulating cell function and fate. It is highly desirable to fabricate biomimetic models to investigate the role of ECM in stem cell differentiation. In this study, arginine- glycine--aspartate （RGD）-modified gold nanoparticles （Au NPs） with tunable surface ligand density were prepared to mimic the ECM microenvironment. Their effect on osteogenic and adipogenic differentiation of human mesenchymal stem cells （MSCs） was investigated. The biomimetic Au NPs were taken up by MSCs in a ligand density-dependent manner. The biomimetic NPs with a high RGD density had an inhibitive effect on the alkaline phosphatase （ALP） activity, calcium deposition, and osteogenic marker gene expression of MSCs. Their effect on oil droplet formation and adipogenic marker gene expression was negative when RGD density was low, while their effect was promotive when RGD density was high. The biomimetic Au NPs regulated the osteogenic and adipogenic differentiation of MSCs mainly through affecting the focal adhesion and cytoskeleton. This study highlights the roles of biomimetic NPs on stem cell differentiation that could provide a meaningful strategy in fabricating functional biomaterials for tissue engineering and biomedical applications.

关键词： arginine-glycine--aspartate(RGD) density biomimetic goldnanopartides (Au NPs) osteogenic differentiation adipogenic differentiation mesenchymal stem cells

Gastrointestinal stromal tumor of the stomach with axillary lymph node metastasis: A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第9期23卷 1720-1724页

作者： naoki Kubo Nobumichi Takeuchi Department of Surgery Ina central HospitalInashiNagano 396-8555Japan

Gastrointestinal stromal tumors (GISTs) are the most common type of gastrointestinal mesenchymal tumors, although metastasis to the perigastric lymph nodes is relatively rare, compared with liver or peritoneal metastasis. In this report, we describe a case of stomach GIST with a solitary simultaneous metastasis in the left axillary lymph node. A 68-year-old man was diagnosed with a large upper-stomach GIST, and computed tomography and positron emission tomography revealed masses in the left axilla and right mediastinum. We did not detect evidence of metastases to the liver, or other sites including the perigastric lymph nodes, although findings from the surgically resected axillary lymph nodes were compatible with GIST metastasis. Treatment using imatinib markedly reduced the gastric and mediastinal lesions, and this response persisted for 3 years. The patient subsequently experienced rapid growth of the gastric lesion without mediastinal or axilla recurrence, which required palliative surgery. Despite continuing medical treatment(sunitinib and regorafenib), the patient died of liver metastases 23 mo after the surgery. Based on our findings, it appears that the axillary lymph nodes can be a potential metastatic site for GIST metastasis.

关键词： Gastrointestinal stromal tumor Axillary Lymph node Metastasis Imatinib

Selective extraction of In(Ⅲ),Ga(Ⅲ) and Zn(Ⅱ) using a novel extractant with phenylphosphinic acid

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2016年第2期24卷 232-236页

作者： Yuji Sasaki naoki Matsuo Tatsuya Oshima Yoshinari Baba Department of Applied Chemistry Faculty of EngineeringUniversity of Miyazaki

A new extractant, [N,N-di（2-ethylhexyl）aminolmethylphenylphosphinic acid （DEAPP）, was synthesized to de- velop the mutual separation techniques of In（Ⅲ）, Ga（Ⅲ） and Zn（Ⅱ）. The extraction selectivity for In（Ⅲ）, Ga（Ⅲ） and Zn（Ⅱ） with DEAPP was higher than that of the commercial phosphorus acid extractants such as D2EHPA and PC-88A. The extraction selectivity for metal ions in 1 mob L 1 aqueous ammonium nitrate solution with DEAPP was in the following order： In（Ⅲ）〉 Ga（Ⅲ）〉 Zn（Ⅱ）. These selective extraction behaviors indicate that the amino moiety of DEAPP plays an important role in the mutual separation of ln（Ⅲ）, Ga（Ⅲ） and Zn（ll）. The extraction equilibria of In（Ⅲ）, Ga（Ⅲ） and Zn（Ⅱ） with DEAPP （ = HR） were expressed by the following reactions： In3＋＋ 2（HR）2 InR3（HR）＋ 3H＋, Ga3＋＋ 1.5（HR）2 ＋ N- = GaR2（HR）（NO3）＋ 2H＋, and Zn2＋ 4- 2（HR）2 ZnR2 （HR） 2 determined 4- 2H＋. The extraction equilibrium constants of In（Ⅲ）, Ga（Ⅲ） and Zn（Ⅱ） with DEAPP were to be Kex, M = 1.7 × 104 [*** 1], 4.17 [（***-1）s], and 1.55 × 10 2 [-], respectively.

关键词： Liquid-liquid extraction Phosphinic acid Indium(Ⅲ) Gallium(Ⅲ) Zinc(Ⅱ) Mutual separation Extraction equilibrium

Van Vleck paramagnetism of europium oxyhydroxide

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2015年第2期33卷 177-181页

作者： Hiroaki Samata naoki Wada Tadashi C.Ozawa Graduate School of Maritime Sciences Kobe University FukaeminamiHigashinada Kobe Hyogo 658-0022 Japan International Center for Materials Nanoarchitectonics National Institute for Materials ScienceNamiki Tsukuba Ibaraki 305-0044 Japan

Europium oxyhydroxide crystals were synthesized by the flux method. The as-grown crystals were transparent and had a plate-like shape with natural flat surfaces. The powder XRD data were refined by assuming a monoclinic structure of the space group P21/m and lattice parameters of a=0.4346 nm, b=0.3744 nm, c=0.6107 nm, and β=108.62°. The magnetic susceptibility of the EuOOH crystals exhibited typical Van Vleck temperature-independent paramagnetism below 120 K. The calculated susceptibility, based on Van Vleck＇s theory, agreed with the experimental data to some extent, with the coupling constant λ=458±10 K. The experimental results were in close agreement with the results calculated using a modified formula with λ=505±2 K and a constant term C=4.6×10^-4 emu/（mol·Oe）.

关键词： europium oxyhydroxide magnetic measurement Van Vleck paramagnetism rare earths

Growth and luminescence properties of Eu-doped HfO2/α-Al2O3 eutectic scintillator

在线全文

学校读者我要写书评

暂无评论

Journal of Rare Earths 2016年第8期34卷 796-801页

作者： Kei Kamada Kosuke Hishinuma Shunsuke Kurosawa Akihiro yamaji Yasuhiro Shoji Jan Pejchal Yuji Ohashi Yuui Yokota Akira Yoshikawa New Industry Creation Hatchery Center Tohoku University6-6-10 AobaAramakiAoba-kuSendaiMiyagi980-8579Japan C&A Corporation T-Biz 6-6-40 AobaAramakiAoba-kuSendaiMiyagi 980-8579Japan Institute for Material Reseach Tohoku University2-1-1 Katahira Aoba-kuSendal Miyagi980-8577Japan Institute of Physics AS CR Cukrovarnicka 1016253 PragueCzech Republic

HfO2/a-A12O3 eutectics were grown by the micro-pulling down （μ-PD） method at the composition of 63.2 mol.% of-A1203, 35.512 mol.% HfO2, 0.92 mol.% Eu203 and 0.368 tool.% Y2O3. Rod phases with around 3-4 μm diameter were observed on the transverse cross-section. The phase-separated scintillator fibers （PSSFs） structure with fibers of around 3-4 μm diameter and 10-20 μm length was observed. The electron back scattering patterns indicated crystal orientation of [101] for monoclinic HfO2 rod phase and [0001] for μt-A1203 matrix in the transverse cross-section. Eu3＋ 4f-4f emission was observed at 550-750 nm.

关键词： eutectic scintillator micro-pulling down method rare earths

Change in microstructural characteristics of laser powder bed fused Al–Fe binary alloy at elevated temperature

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2022年第2期97卷 38-53页

作者： Xing Qi naoki Takata Asuka Suzuki Makoto Kobashi Masaki Kato Department of Materials Process Engineering Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Aichi Center for Industry and Science Technology 1267-1 AkiaiYakusa-choToyota 470-0356Japan

The present study addressed the change in the microstructure of Al-2.5 wt%Fe binary alloy produced using laser powder bed fusion(L-PBF)technique by thermal exposure at 300℃,and the associated mechanical and thermal properties were systematically examined as ***-semi-cylindrical patterns corresponding to melt pools in the microstructure were macroscopically observed for the asmanufactured *** change in the melt-pool morphology was observed after thermal exposure for1000 *** the melt pools,a large number of the nanoscale metastable Al6 Fe phase particles were uniformly distributed inside columnar grains of theα-Al matrix containing concentrated solute Fe in *** sequential formation and coarsening of stableθ-Al13 Fe4 phases were observed upon exposure to a 300℃ environment,but a considerable amount of nano-sized metastable Al6 Fe phases remained even after 1000 ***,the thermal exposure continuously reduced the concentration of solute Fe atoms in theα-Al *** significant grain growth was found inα-Al matrix after 1000 h owing to the pinning effect of the dispersed fine particles on grain boundary *** results demonstrate a sluggish change in microstructural morphologies of the Al-2.5 wt%Fe *** quantified microstructural parameters addressed dominant strengthening contributions by the solid solution of Fe element and Orowan strengthening mechanism by fine Al-Fe intermetallics in the L-PBF-produced *** high strength level was sustained even after being exposed to 300℃ for long *** superior balance of mechanical properties and thermal conductivity can be achieved in the experimental alloys by taking advantage of the various microstructural parameters related to the Al-Fe intermetallic phases andα-Al matrix.

关键词： Al-Fe alloy Laser powder bed fusion Phase transformation Microstructure Tensile properties

Engineering W/O/W pickering emulsion for the enhanced antigen delivery and immune responses

在线全文

学校读者我要写书评

暂无评论

Particuology 2024年第9期92卷 224-233页

作者： Sihua Wu Xiaonan Huang Yin Ma naoki Asakawa Yufei Xia State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing100081China Key Laboratory of Biopharmaceutical Preparation and Delivery Chinese Academy of SciencesBeijing100190China Division of Molecular Science Graduate School of Science and TechnologyGunma University1-5-1Tenjin-choKiryu376-8515Japan Sinovac Biotech Ltd. Beijing100085China School of Chemical Engineering Nanjing Tech UniversityNanjing211816China School of Chemical Engineering University of Chinese Academy of SciencesBeijing100049China

Tumor immunotherapy,particularly cancer vaccines,holds promise for combating cancer by harnessing tailored immune responses against malignant ***,conventional approaches face challenges in efficiently delivering antigens for optimal immune *** adjuvants,like MF59,enhance cellular uptake but struggle to induce robust CD8^(+)T cell ***,we introduce a novel strategy employing a water-in-oil-in-water(W/O/W)multiple Pickering emulsion(mPE)for antigen *** mPE,utilizing biocompatible,pH-sensitive particles,encapsulates antigens within the inner water phase,ensuring enhanced intracellular processing and dictating the intracellular fate of antigens for improved *** vitro and in vivo studies demonstrated that mPEs induced robust dendritic cells activation and antigen cross-presentation,leading to a significantly enhanced immune ***,calcium phosphate-stabilized mPE(CaP-mPE)illustrated the more robust IFN-γ^(+)T cell *** comparison with traditional surfactant-stabilized multiple emulsions,CaP-mPE significantly inhibit tumor growth and effectively prolong the survival of tumor-bearing *** innovative approach offers a promising avenue for the development of effective cancer vaccines with potent cellular immune responses.

关键词： Cancer vaccine Multiple pickeringemulsion Adjuvant Antigen delivery Anti-tumor response

Characterization of Kiwifruit Xyloglucan

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2009年第10期51卷 933-941页

作者： Xingjun Li naoki Sakurai Donald J. Nevins Academy of the State Administration of Grains Beijing 100037 China Graduate School of Biosphere Science Hiroshima University Kagamiyama 1-7-1 Higashi-hiroshima 739-8521 Japan Department of Plant Sciences University of California Davis CA 95616 USA

Structural characteristics of xyloglucan are constant in the pericarp cell walls of kiwifruit （Actinidia deliciosa） throughout fruit enlargement and maturation. Most of the xyioglucan （XG） persists in the cell walls of ripe kiwifruit. XG from the pericarp tissues of 36-h ethylene-treated kiwifruit was extracted as hemicellulose Ⅱ （HC-Ⅱ） with 4.28 M KOH containing 0.02% NaBH4, and purified using iodine precipitation and subsequent anion-exchange chromatography. This purifying protocol increased XG purity from 50 mol% in HC-Ⅱ fraction to 62 mol% in the purified XG powder. The molar ratio of glucose： xylose： galactose： fucose in the purified XG was 10： 6.9： 2.1： 0.3. Gel permeation chromatography indicated that purified XG had an average molecular-mass of 161 KDa, a value that exceeds the 95 KDa Mr determined for total polymeric sugars. Sugar linkage analysis confirmed the lack of fucose in the kiwifruit XG, but a small amount of arabinoxylan and low Mr glucomannan remained associated with this fraction.

关键词： Actinidia deliciosa cell walls glucomannan hemicellulose sugar linkage analysis xyloglucan

Cyr61 is a potential prognostic marker for prostate cancer

在线全文

学校读者我要写书评

暂无评论

Asian Journal of Andrology 2012年第3期14卷 405-408,I0006页

作者： naoki Terada Prakash Kulkarni Robert H Getzenberg Department of Urology the James Buchanan Brady Urological Institute The Johns Hopkins University School of Medicine Baltimore M D 21287 USA Department of Urology Oncology The Johns Hopkins University School of Medicine Baltimore Baltimore MD 21287 USA Department of Pharmacology and Molecular Sciences The Johns Hopkins University School of Medicine Baltimore MD 21287 USA

Cysteine-rich angiogenic inducer 61 （Cyr61） is an extracellular matrix protein involved in the transduction of growth factor and hormone signaling that is frequently altered in expression in several types of cancers. In prostate cancer （PCa）, Cyr61 is highly expressed in organ-confined disease. Further, Cyr61 expression levels are associated with a lower risk of disease recurrence, and can be quantitatively measured in the serum. Considered together, these results indicate that Cyr61 is a potential and clinically useful tissue, as well as serum-based biomarker for differentiating lethal and non-lethal PCa.

关键词： Cyr61 lethal prostate cancer non-lethal prostate cancer overdiagnosis overtreatment prostate cancer