检索结果-南通市图书馆

Monitoring and mathematical modeling of mitochondrial ATP in myotubes at single-cell level reveals two distinct population with different kinetics

引用

Quantitative Biology 2020年第3期8卷 228-237页

作者： naoki matsuda Ken-ichi Hironaka Masashi Fujii Takumi Wada Katsuyuki Kunida Haruki Inoue Miki Eto Daisuke Hoshino Yasuro Furuichi Yasuko Manabe Nobuharu LFujii Hiroyuki Noji Hiromi Imamura Shinya Kuroda Department of Biological Sciences Graduate School of ScienceUniversity of TokyoTokyo 113-0033Japan Molecular Genetics Research Laboratory Graduate School of ScienceUniversity of TokyoTokyo 113-0033Japan Department of Mathematical and Life Sciences Graduate School of Integrated Sciences for LifeHiroshima UniversityHiroshima 739-8526Japan Laboratory of Computati onal Biology Graduate School of Biological SciencesNara Institute of Science and Tech no logyNara 630-0192Japan Department of Computational Biology and Medical Sciences Graduate School of Frontier SciencesUniversity of TokyoTokyo 113-0033Japan Department of Engineering Science Graduate School of Informatics and EngineeringUniversity of Electro-CommunicationsTokyo 182-8585Japan Department of Health Promotion Sciences Graduate School of Human Health SciencesTokyo Metropolitan UniversityTokyo 192-0397Japan Department of Applied Chemistry Graduate School of EngineeringUniversity of TokyoTokyo 113-8656Japan Department of Functional Biology Graduate School of BiostudiesKyoto UniversityKyoto 606-8501Japan CREST Japan Science and Technology CorporationTokyo 113-0033Japan

Backgrounds ATP is the major energy source for myotube contraction,and is quickly produced to compensate ATP consumption and to maintain sufficient ATP *** is consumed mainly in cytoplasm and produced in mitochondria during myotube *** understand the mechanism of ATP homeostasis during myotube contraction,it is essential to monitor mitochondrial ATP at single-cell level,and examine how ATP is produced and consumed in ***:We established C2C12 cell line stably expressing fluorescent probe of mitochondrial ATP,and induced differentiation into myotubes・We gave electric pulse stimulation to the differentiated myotubes,and measured mitochondrial *** constructed mathematical model of mitochondrial ATP at single-cell level,and analyzed kinetic parameters of ATP production and ***:We performed hierarchical clustering analysis of time course of mitochondrial ATP,which resulted in two *** 1 showed strong transient increase,whereas cluster 2 showed weak transient *** modeling at single-cell level revealed that the ATP production rate of cluster 1 was larger than that of cluster 2,and that both regulatory pathways of ATP production and consumption of cluster 1 were faster than those of cluster *** 1 showed larger mitochondrial mass than cluster 2,suggesting that cluster 1 shows the similar property of slow muscle fibers,and cluster 2 shows the similar property of fast muscle *** Cluster 1 showed the stronger mitochondrial ATP increase by larger ATP production rate,but not smaller *** 1 might reflect the larger oxidative capacity of slow muscle fiber.

关键词： mitocondrial ATP production ATP consumption single-cell analysis mathematical modeling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

In vivo direct neuronal conversion as a therapeutic strategy for ischemic stroke

引用

Neural Regeneration Research 2025年第8期20卷 2309-2310页

作者： Takashi Irie Taito matsuda Department of Stem Cell Biology and Medicine Graduate School of Medical SciencesKyushu UniversityFukuokaJapan Department of Neurology Neurological InstituteGraduate School of Medical SciencesKyushu UniversityFukuokaJapan

Stroke causes neuronal loss,which ultimately results in persistent neurological ***,stroke was the third-leading cause of death and disability combined in all ages in 2019,after neonatal disorders and ischemic heart *** that year,there were 12.2 million incident strokes,101 million prevalent strokes,and 143 million disability-adjusted life-years due to stroke.

关键词： neonatal death therapeutic

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Performance of 18-fluoro-2-deoxyglucose positron emission tomography for esophageal cancer screening

引用

World Journal of Gastroenterology 2017年第15期23卷 2743-2749页

作者： Masau Sekiguchi Takashi Terauchi Yasuo Kakugawa naoki Shimada Yutaka Saito Takahisa matsuda Cancer Screening Center National Cancer Center HospitalTokyo Endoscopy Division National Cancer Center HospitalTokyo Division of Screening Technology Center for Public Health SciencesNational Cancer CenterTokyo Department of Diagnostic Radiology National Cancer Center HospitalTokyo Department of Nuclear Medicine Cancer Institute HospitalJapanese Foundation for Cancer Research Department of Radiological Technology Radiological Diagnosis National Cancer Center HospitalTokyo

AIM To evaluate the performance of 18-fluoro-2-deoxyglucose positron emission tomography(FDG-PET) for esophageal cancer(EC) *** We retrospectively analyzed the data of consecutive asymptomatic individuals who underwent FDG-PET and esophagogastroduodenoscopy(EGD) simultaneously for cancer screening at our institution from February 2004 to March 2013. In total, 14790 FDG-PET and EGD procedures performed for 8468 individuals were included in this study, and the performance of FDGPET for EC screening was assessed by comparing the results of FDG-PET and EGD, considering the latter as the *** Thirty-two EC lesions were detected in 28 individuals(31 squamous cell carcinomas and 1 adenocarcinoma). The median tumor size was 12.5 mm, and the depths of the lesions were as follows: Tis(n = 12), T1a(n = 15), and T1b(n = 5). Among the 14790 FDG-PET procedures, 51 examinations(0.3%) showed positive findings in the esophagus; only 1 was a true-positive finding. The screen sensitivity, specificity, positive predictive value, and negative predictive value of FDGPET for ECs were 3.6%(95%CI: 0.1-18.3), 99.7%(95%CI: 99.6-99.7), 2.0%(95%CI: 0.0-10.4), and 99.8%(95%CI: 99.7-99.9), respectively. Of the 50 FDG-PET false-positive cases, 31 were observed in the lower esophagus, and gastroesophageal reflux disease was observed in 17 of these 31 *** This study is the first to clarify the FDG-PET performance for EC screening. Based on the low screen sensitivity, FDG-PET is considered to be difficult to use as a screening modality for ECs.

关键词： Cancer screening Esophageal cancer Esophagogastroduodenoscopy Positron emission tomography Screen sensitivity

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparison of cerebral activation between motor execution and motor imagery of self-feeding activity

引用

Neural Regeneration Research 2021年第4期16卷 770-774页

作者： Moemi Matsuo naoki Iso Kengo Fujiwara Takefumi Moriuchi Daiki matsuda Wataru Mitsunaga Akira Nakashima Toshio Higashi Unit of Medical Science Nagasaki University Graduate School of Biomedical SciencesNagasakiJapan Center for Child Mental Health Care and Education Nagasaki UniversityNagasakiJapan Department of Rehabilitation Faculty of Health SciencesTokyo Kasei UniversitySaitamaJapan Zeshinkai General Incorporated Association Nagasaki Rehabilitation HospitalNagasakiJapan The Japanese Red Cross Nagasaki Genbaku HospitalNagasakiJapan Unit of Rehabilitation Nagasaki University HospitalNagasakiJapan

Motor imagery is defined as an act wherein an individual contemplates a mental action of motor execution without apparent *** practice executed by repetitive motor imagery can improve motor performance without simultaneous sensory input or overt *** aimed to investigate cerebral hemodynamics during motor imagery and motor execution of a self-feeding activity using *** study included 21 healthy right-handed *** self-feeding activity task comprised either motor imagery or motor execution of eating sliced cucumber pickles with chopsticks to examine eight regions of interest:pre-supplementary motor area,supplementary motor area,bilateral prefrontal cortex,premotor area,and sensorimotor *** mean oxyhemoglobin levels were detected using near-infrared spectroscopy to reflect cerebral *** mean oxyhemoglobin levels during motor execution were significantly higher in the left sensorimotor cortex than in the supplementary motor area and the left premotor ***,significantly higher oxyhemoglobin levels were detected in the supplementary motor area and the left premotor area during motor imagery,compared to motor *** motor area and premotor area had important roles in the motor imagery of self-feeding ***,the activation levels of the supplementary motor area and the premotor area during motor execution and motor imagery are likely affected by intentional cognitive *** of cerebral activation differed in some areas during motor execution and motor imagery of a self-feeding *** study was approved by the Ethical Review Committee of Nagasaki University(approval No.18110801)on December 10,2018.

关键词： Activities of Daily Living brain function hemodynamics imagery(psychotherapy) mental practice motor cortex near-infrared neuroimaging neuroscience rehabilitation spectroscopy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Perforation associated with endoscopic submucosal dissection for duodenal neoplasm without a papillary portion

引用

World Journal of Gastrointestinal Surgery 2017年第7期9卷 161-166页

作者： Yasuhiro matsuda Kazuki Sakamoto naoki Kataoka Tomoyuki Yamaguchi Masafumi Tomita Shinichiro Makimoto Department of Surgery Kishiwada Tokushukai HospitalKishiwada CityOsaka 596-8522Japan

AIM To investigate predictors of perforation after endoscopic resection(ER) for duodenal neoplasms without a papillary *** This was a single-center, retrospective, cohort study conducted between April 2003 and September 2014. A total of 54 patients(59 lesions) underwent endoscopic mucosal resection(EMR)(n = 36) and endoscopic submucosal dissection(ESD)(n = 23). Clinical features, outcomes, and predictors of perforation were *** Cases of perforation occurred in eight(13%) patients(95%CI: 4.7%-22.6%). Three ESD cases required sur-gical management because they could not be repaired by clipping. Delayed perforation occurred in two ESD cases, which required surgical management, although both patients underwent prophylactic clipping. All patients with perforation who required surgery had no postoperative complications and were discharged at anaverage of 13.2 d after ER. Perforation after ER showed a significant association with a tumor size greater than20 mm(P = 0.014) and ESD(P = 0.047).CONCLUSION ESD for duodenal neoplasms exceeding 20 mm may be associated with perforation. ESD alone is not recom-mended for tumor treatment, and LECS should be con-sidered as an alternative.

关键词： Duodenal neoplasm Endoscopic submucosal dissection Laparoscopic and endoscopic cooperative surgery

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Molecular mechanism underlying the functional loss of cyclindependent kinase inhibitors p16 and p27 in hepatocellular carcinoma

引用

World Journal of Gastroenterology 2008年第11期14卷 1734-1740页

作者： Yasunobu matsuda Department of Medical Technology NiigataUniversity Graduate School of Health SciencesAsahimachi-dori2-746Niigata 9518518Japan

Hepatocellular carcinoma （HCC） is one of the most common human cancers, and its incidence is still increasing in many countries. The prognosis of HCC patients remains poor, and identification of useful molecular prognostic markers is required. Many recent studies have shown that functional alterations of cellcycle regulators can be observed in HCC. Among the various types of cell-cycle regulators, p16 and p27 are frequently inactivated in HCC and are considered to be potent tumor suppressors, p16, a G1-specific cell-cycle inhibitor that prevents the association of cyclindependent kinase （CDK） 4 and CDK6 with cyclin DI, is frequently inactivated in HCC via CpG methylation of its promoter region, p16 may be involved in the early steps of hepatocarcinogenesis, since p16 gene methylation has been detected in subsets of pre-neoplastic liver cirrhosis patients, p27, a negative regulator of the G1-S phase transition through inhibition of the kinase activities of Cdk2/cyclin A and Cdk2/cyclin E complexes, is now considered to be an adverse prognostic factor in HCC. In some cases of HCC with increased cell proliferation, p27 is overexpressed but inactivated by sequestration into cyclin D1-CDK4-containing complexes. Since loss of p16 is closely related to functional inactivation of p27 in HCC, investigating both p16 and p27 may be useful for precise prognostic predictions in individuals with HCC.

关键词： Hepatocellular carcinoma Cell-cycle regulator Cyclin-dependent kinase inhibitor DNA methylation DNA methyltransferase p16 p27 FoxM1b

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Weakly Closed Graphs and F-Purity of Binomial Edge Ideals

引用

Algebra Colloquium 2018年第4期25卷 567-578页

作者： Kazunori matsuda Kitami Institute of Technology Kitami

Herzog,Hibi,Hreinddttir et *** the class of closed graphs,and they proved that the binomial edge ideal JG of a graph G has quadratic GrSbner bases if G is *** this paper,we introduce the class of weakly closed graphs as a generalization of the closed graph,and we prove that the quotient ring S/JG of the polynomial ring S=K[x1,...,xn,y1,...,yn]with K a field and n=|V(G)|is F-pure if G is weakly *** fact is a generalization of Ohtani's theorem.

关键词： binomial edge ideal closed graph weakly closed graph F-pure

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Full-scale dynamic testing of response-controlled buildings and their components:concepts,methods,and findings

引用

Earthquake Engineering and Engineering Vibration 2014年第S1期13卷 167-181页

作者： Kazuhiko Kasai Kazuhiro matsuda Structural Engineering Research Center Tokyo Institute of Technology Director and Professor Assistant Professor

A series of shake-table tests was conducted by inserting and replacing 4 different types of dampers,or by removing them in a full-scale 5-story steel frame building. The objective is to validate response-control technologies that are increasingly adopted for major Japanese buildings without being attested to-date by a major earthquake. Test results are briefly described,and good performance of the dampers and frame demonstrated. The concepts of the full-scale building tests and various contributions are discussed. The difficulty associated with full-scale dynamic testing is explained.

关键词： response-controlled building steel frame steel damper viscous damper oil damper viscoelastic damper

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Recent advance in the structural analysis of HIV-1 envelope protein

引用

Science China(Life Sciences) 2015年第5期58卷 420-424页

作者： matsuda Zene Research Center for Asian Infectious Diseases Institute of Medical Science the University of Tokyo Laboratory of Structural Virology and Immunology Institute of Biophysics Chinese Academy of Sciences

Human immunodeficiency virus type 1 （HIV-1）, a causative agent of AIDS, is affecting today more than 35 millions of people worldwide. The advance of anti-HIV chemotherapy has made AIDS a chronic non-fatal disease in resourceful countries. Longawaited anti-HIV-1 vaccine is still not with us yet; however, great progress in structural analyses of the envelope protein of HIV-1 in recent years starts to shed light on rational intervention targeted at the envelope protein, as will be reviewed in this article.

关键词： HIV-1 envelope glycuprotein CryoEM structure membrane fusion vaccine

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

3D reconstruction size effect on the quantification of solid oxide fuel cell nickel-yttria-stabilized-zirconia anode microstructural information using scanning electron microscopy-focused ion beam technique

引用

Science Bulletin 2016年第17期61卷 1317-1323页

作者： Zhenjun Jiao naoki Shikazono Institute of Industrial Science The University of Tokyo

Self-made conventional nickel-yttria-stabilized zirconia composite anodes after reduction and 500 h operation were analyzed by three-dimensional microstructure reconstruction based on focused ion beam-scanning electron microscopy *** area,threephases-boundary density and phase volume fraction were measured based on the three-dimensional microstructure reconstruction to quantitatively study the statistical characterization of solid oxide fuel cell nickel-yttria-stabilizedzirconia anode microstructure before and after *** is found that for anode operated with long time,it is necessary to increase the corresponding three-dimensional reconstruction size to suppress the influence of microstructure variation caused by Ni agglomeration in order to obtain more accurate microstructural quantification information.

关键词： FIB-SEM 3D reconstruction TPB density Tetragonal mesh

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：