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脂质转移蛋白SiLTP1基因参与谷子耐盐响应初探

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作物学报 2025年第1期51卷 58-67页

作者：孟凡花刘敏沈傲刘炜山东省农业科学院农作物种质资源研究所山东济南250100 上海应用技术大学生态技术与工程学院上海201418

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁... 详细信息

土壤盐渍化严重制约农业生产发展,通过提高植物耐盐性进行盐碱地综合利用,对于保障粮食安全,提升农业生产效率具有重要意义。脂质转移蛋白是一类在高等植物中广泛存在的小分子蛋白质,能够参与植株生长、信号转导以及多种生物和非生物胁迫过程。前期本课题组克隆到一个谷子中编码脂质转移蛋白的基因SiLTP1,本研究通过构建其原核表达载体和植物双元过表达载体,获得了该基因的原核表达蛋白及同源转化过表达阳性植株,经进一步筛选获得4个纯合株系。体外耐盐性试验显示, SiLTP1的原核表达蛋白具有一定耐盐性;转基因植株苗期耐盐生理指标测定显示,SiLTP1过表达植株在遭受盐胁迫时,植株体内MDA积累减少,抗氧化酶含量升高,可积累更少的过氧化氢,从而降低胁迫对植株的氧化损伤,显示基因过表达植株具有更好的耐盐性。本研究结果初步揭示, SiLTP1可正向调控谷子的耐盐性,其在谷子耐盐抗逆方面具有潜在功能,为耐盐谷子品种改良及新品种培育提供了理论基础和候选基因资源。

关键词：脂质转移蛋白 SiLTP1 盐胁迫谷子耐盐响应

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Global view of bionetwork dynamics:adaptive landscape

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Journal of Genetics and Genomics 2009年第2期36卷 63-73页

作者： Ping ao Department of Mechanical Engineering and Department of Physics University of Washington Seattle WA 98195 USA Shanghai Center for Systems Biomedicine Key Laboratory of Systems Biomedicine of Ministry of Education Shanghai Jiao Tong University Shanghai 200240 China

Based on recent work, I will give a nontechnical brief review of a powerful quantitative concept in biology, adaptive landscape, ini- tially proposed by S. Wright over 70 years ago, reintroduced by one of the founders of molecular biology and by others in different bio- logical contexts, but apparently forgotten by modem biologists for many years. Nevertheless, this concept finds an increasingly important role in the development of systems biology and bionetwork dynamics modeling, from phage lambda genetic switch to endogenous net- work for cancer genesis and progression. It is an ideal quantification to describe the robustness and stability of bionetworks. Here, I will first introduce five landmark proposals in biology on this concept, to demonstrate an important common thread in theoretical biology. Then I will discuss a few recent results, focusing on the studies showing theoretical consistency of adaptive landscape. From the perspec- tive of a working scientist and of what is needed logically for a dynamical theory when confronting empirical data, the adaptive landscape is useful both metaphorically and quantitatively, and has captured an essential aspect of biological dynamical processes. Though at the theoretical level the adaptive landscape must exist and it can be used across hierarchical boundaries in biology, many associated issues are indeed vague in their initial formulations and their quantitative realizations are not easy, and are good research topics for quantitative biologists. I will discuss three types of open problems associated with the adaptive landscape in a broader perspective.

关键词： adaptive landscape stochastic dynamics, bionetworks systems biology

Measurement and correlation of solid–liquid phase equilibria for binary and ternary systems consisting of N-vinylpyrrolidone, 2-pyrrolidone and water

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Chinese Journal of Chemical Engineering 2018年第4期26卷 806-811页

作者： ao Su Sifang Li Department of Chemical and Biochemical Engineering College of Chemistry and Chemical Engineering Xiamen University

The solid–liquid equilibria(SLE)for binary and ternary systems consisting of N-Vinylpyrrolidone(NVP),2-Pyrrolidone(2-P)and water are *** phase diagrams of NVP(1)+2-P(2),NVP(1)+water(2),NVP(1)+2-P(2)+1 wt%water(3)and NVP(1)+2-P(2)+2 wt%water(3)are identified as simple eutectic type with the eutectic points at 263.75 K(x_(1E)=0.5427),251.65 K(x_(1E)=0.3722),260.25 K(x_(1E)=0.5031)and256.55 K(x_(1E)=0.4684),*** phase diagram of 2-P(1)+water(2)has two eutectic points(x_(1E)=0.1236,T_E=259.15 K and x_(1E)=0.7831,T_E=286.15 K)and one congruent melting point(x_(1C)=0.4997,T_C=303.55 K)because of the generation of a congruently melting addition compound:2-P·H_*** ideal solubility and the UNIFAC models were applied to predict the SLE,while the Wilson and NRTL models were employed in correlating the experimental *** best correlation of the SLE data has been obtained by the Wilson model for the binary system of NVP+*** UNIFAC model gives more satisfactory predictions than the ideal solubility model.

关键词： Phase equilibria Thermodynamics Model N-vinylpyrrolidone 2-Pyrrolidone Water

Is philosophy of any use for scientists？

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Science Bulletin 2018年第16期63卷 1017-1018页

作者： Ping ao Shanghai Center for Quantitative Life Sciences and Department of Physics Shanghai University.Shanghai 200444 China

This is a question which would certainly divide physicists in particular and scientists in *** aware of that,here I would like to take the successful career path of an excellent physicist as a positive example,supplemented by my own humble and negligible experience.I do hope that a bit of such discussion would be useful in the current rash state of scientific research in China.

关键词：科学家哲学物理学家科学研究

Emerging of Stochastic Dynamical Equalities and Steady State Thermodynamics from Darwinian Dynamics

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Communications in Theoretical Physics 2008年第5期49卷 1073-1090页

作者： P.ao Department of Mechanical Engineering and Department of Physics University of WashingtonSeattleWA 98195USA

The evolutionary dynamics first conceived by Darwin and Wallace, referring to as Darwinian dynamics in the present paper, has been found to be universally valid in biology. The statistical mechanics and thermodynamics, while enormous successful in physics, have been in an awkward situation of wanting a consistent dynamical understanding. Here we present from a formal point of view an exploration of the connection between thermodynamics and Darwinian dynamics and a few related topics. We first show that the stochasticity in Darwinian dynamics implies the existence temperature, hence the canonical distribution of Boltzmann-Gibbs type. In term of relative entropy the Second Law of thermodynamics is dynamically demonstrated without detailed balance condition, and is valid regardless of size of the system. In particular, the dynamical component responsible for breaking detailed balance condition does not contribute to the change of the relative entropy. Two types of stochastic dynamical equalities of current interest are explicitly discussed in the present approach： One is based on Feynman-Kac formula and another is a generalization of Einstein relation. Both are directly accessible to experimental tests. Our demonstration indicates that Darwinian dynamics represents logically a simple and straightforward starting point for statistical mechanics and thermodynamics and is complementary to and consistent with conservative dynamics that dominates the physical sciences. Present exploration suggests the existence of a unified stochastic dynamical framework both near and far from equilibrium.

关键词： non-equilibrium processes Darwinian dynamics generalized Einstein relation absence of detailed balance stochastic dynamical equalities

Novel Primate Bocaparvovirus Species 3 Identified in Wild Macaca Mulatta in China

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Virologica Sinica 2020年第1期35卷 34-42页

作者： Yuanyun ao Zhaojun Duan Chinese Center for Disease Control and Prevention National Institute for Viral Disease Control and PreventionBeijingChina

Primate bocaparvovirus(BOV) is a possible cause of respiratory disorders and gastroenteritis in humans. However, the diversity and evolution of these viruses remain largely unknown, despite the identification of a growing number of BOVs in non-human primates(NHPs). Here, we report the identification of a novel BOV(provisionally named Macaca mulatta bocaparvovirus [MmBOV]) in the feces of wild Macaca mulatta in China by viral metagenomic analysis. Seven of 400 fecal samples from Macaca mulatta were positive for MmBOV. An almost complete genome sequence of 4,831 nucleotides was obtained, which had genomic organization and protein motifs similar to human bocaviruses(HOBVs), and shared characteristically low G/C content and weak codon usage bias. Sequence analyses of NS1, NP1, and VP1 revealed that Mm BOV was most closely related to HBOV4 of Primate bocaparvovirus 2(approximately 68.4%/70.6%, 73.3%/67.6%, and 70.4%/73.1% nucleotide/amino acid identities, respectively). Additionally, phylogenetic analysis revealed that Mm BOV formed an independent peripheral branch, but clustered closely with those of the Primate bocaparvovirus species in the BOV genus(particularly HBOV4). These data strongly suggest that HBOV4 originated from NHP bocaparvoviruses around 200–300 years ago, and that NHPs may act as HBOV reservoirs. Following the International Committee of Taxonomy for Viruses guidelines, we propose Mm BOV as a new species(tentatively named Primate bocaparvovirus 3) in the genus Bocaparvovirus, which is the first report of a novel species of primate BOV. Our data facilitate future research on the genetic diversity and evolution of primate bocaparvoviruses and highlight the importance of bocaparvovirus surveys in wild NHPs.

关键词： Primate bocaparvovirus(BOV) Novel species Macaca mulatta Sequence analysis Phylogenetic analysis

A new segmentation algorithm for lunar surface terrain based on CCD images

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Research in Astronomy and Astrophysics 2015年第9期15卷 1604-1612页

作者： Hong-Kun Jiang Xiao-Lin Tian ao-ao Xu Lunar and Planetary Science Laboratory/Space Science Institute Macao University of Science and Technology

Terrain classification is one of the critical steps used in lunar geomorphologic analysis and landing site selection. Most of the published works have focused on a Digital Elevation Model （DEM） to distinguish different regions of lunar terrain. This paper presents an algorithm that can be applied to lunar CCD images by blocking and clustering according to image features, which can accurately distinguish between lunar highland and lunar mare. The new algorithm, compared with the traditional algo- rithm, can improve classification accuracy. The new algorithm incorporates two new features and one Tamura texture feature. The new features are generating an enhanced image histogram and modeling the properties of light reflection, which can represent the geological characteristics based on CCD gray level images. These features are ap- plied to identify texture in order to perform image clustering and segmentation by a weighted Euclidean distance to distinguish between lunar mare and lunar highlands. The new algorithm has been tested on Chang＇e-1 CCD data and the testing result has been compared with geological data published by the U.S. Geological Survey. The result has shown that the algorithm can effectively distinguish the lunar mare from highlands in CCD images. The overall accuracy of the proposed algorithm is satisfactory, and the Kappa coefficient is 0.802, which is higher than the result of combining the DEM with CCD images.

关键词： Moon -- methods: data analysis -- techniques: image processing

Testing High-Dimensional Nonparametric Behrens-Fisher Problem

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Journal of Systems Science & Complexity 2022年第3期35卷 1098-1115页

作者： MENG Zhen LI Na YUAN ao Academy of Mathematics and Systems Science Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China Department of Biostatistics Bioinformatics and BiomathematicsGeorgetown UniversityWashington 20057USA

For high-dimensional nonparametric Behrens-Fisher problem in which the data dimension is larger than the sample size,the authors propose two test statistics in which one is U-statistic Rankbased Test(URT)and another is Cauchy Combination Test(CCT).CCT is analogous to the maximumtype test,while URT takes into account the sum of squares of differences of ranked samples in different dimensions,which is free of shapes of distributions and robust to *** asymptotic distribution of URT is derived and the closed form for calculating the statistical significance of CCT is *** simulation studies are conducted to evaluate the finite sample power performance of the statistics by comparing with the existing *** simulation results show that our URT is robust and powerful method,meanwhile,its practicability and effectiveness can be illustrated by an application to the gene expression data.

关键词： Cauchy combination test nonparametric Behrens-Fisher problem rank-based test U-statistic

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C@Ag core‒shell structure as lubricating additives towards high efficient lubrication

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Friction 2024年第9期12卷 1991-2003页

作者： Dong ao Xiaoqiang FAN Minhao ZHU Key Laboratory of Advanced Technologies of Materials(Ministry of Education) School of Materials Science and EngineeringSouthwest Jiaotong UniversityChengdu 610031China Tribology Research Institute School of Mechanical EngineeringSouthwest Jiaotong UniversityChengdu 610031China

Efficient cooperative lubrication can be achieved via the introduction of core‒shell structure lubricant additives with hard core and soft shell,for obtaining the expected anti-wear performance from the structural changes in the friction *** this study,C@Ag microspheres with a core‒shell structure were prepared by the redox method with carbon spheres as the core and Ag nanoparticles as the *** tribological behaviors as base oil(G1830)additive with different concentrations were investigated in *** with base oil,the addition of C@Ag particles at 0.5 wt%can reduce the coefficient of friction(COF)and wear volume(Wv)up to 15.5%and 88%,*** importantly,C@Ag particles provide superior lubrication performance to single additive(like carbon sphere(CS)and Ag nanoparticle).C@Ag core‒shell particles contribute to the formation of tribo-film by melt bonding of flexible Ag and carbon sphere(CS)toward excellent self-repair performance and high-efficiency ***,core‒shell structural nanoparticles with hard-core and soft-shell hold bright future for high-performance lubrication application.

关键词： C@Ag microspheres tribo-film self-repair performance high-efficiency lubrication