检索结果-南通市图书馆

A monoclonal antibody against Lates calcarifer vitellogenin and a competitive ELISA to evaluate vitellogenin induction after exposure to xenoestrogen

引用

Journal of Environmental Sciences 2019年第1期31卷 325-333页

作者： Witchuda Prasatkaew Phochit Nanthanawat nanthika khongchareonporn Sutin Kingtong Environmental Science program Faculty of Science Burapha University Department of Biotechnology Faculty of Science Burapha University The Institute of Biotechnology and Genetic Engineering Chulalongkorn University Department of Biology Faculty of Science Burapha University

A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol(E_2: 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry(LC-MS/MS). Antibody was raised in mice by injection of purified VTG. After monoclonal antibody production, the hybridoma clone No. 41(MAb-sea bass VTG 41)was selected and developed for quantification of VTG by competitive enzyme-linked immunosorbent assay(ELISA). The ELISA method was sensitive with a detection limit of VTG 40 ng/mL. MAb-sea bass VTG 41 was specific to VTG from E_2-treated sea bass and others EDCs(Nonylphenol, Benzo[a]pyrene and CdCl_2). Moreover, cross-reactivity was also found in E_2-treated coral grouper(Epinephelus corallicola). The ELISA method obtained from this work can be further applied for the assessment of EDCs in Thailand and Southeast Asia's aquatic environment.

关键词： Monoclonal antibody Vitellogenin Biomarker Endocrine disrupting chemicals(EDCs) ELISA

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel ractopamine–protein carrier conjugation and its application to the lateral flow strip test for ractopamine detection in animal feed

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2019年第2期20卷 193-204页

作者： Pattarachaya PREECHAKASEDKIT Nattaya NGAMROJANAVANICH nanthika khongchareonporn Orawon CHAILAPAKUL Program in Biotechnology Faculty of ScienceChulalongkorn UniversityPatumwanBangkok 10330Thailand Electrochemistry and Optical Spectroscopy Center of Excellence(EOSCE) Department of ChemistryFaculty of ScienceChulalongkorn UniversityPatumwanBangkok 10330Thailand The Institute of Biotechnology and Genetic Engineering Chulalongkorn UniversityPatumwanBangkok 10330Thailand Program in Biotechnology Faculty of ScienceChulalongkorn UniversityPatumwanBangkok 10330Thailand 2Electrochemistry and Optical Spectroscopy Center of Excellence(EOSCE)Department of ChemistryFaculty of ScienceChulalongkorn UniversityPatumwanBangkok 10330Thailand 3The Institute of Biotechnology and Genetic EngineeringChulalongkorn UniversityPatumwanBangkok 10330Thailand 4Nanotec-CU Center of Excellence on Food and AgricultureChulalongkorn UniversityPatumwanBangkok 10330Thailand 4Nanotec-CU Center of Excellence on Food and AgricultureChulalongkorn UniversityPatumwanBangkok 10330Thailand

In this work,a novel conjugate of ractopamine and bovine serum albumin(RAC–BSA)has been developed via the Mannich reaction,with a mole coupling ratio for RAC–BSA of 9:*** proposed conjugation method provides a simple and one-step method with the use of fewer reagents compared with other conjugation methods for competitive ***–BSA conjugation was used to fabricate a competitive lateral flow strip test for RAC detection in animal *** sample preparation,RAC was spiked in swine feed purchased from the local markets in Thailand,and methanol and running buffer at a volume ratio of 10:90 was used as extraction *** procedures for sample preparation were completed within 25 *** optimal conditions,the limit of detection(LOD),assessed by the naked eye within 5 min,was found to be 1 ng/g.A semi-quantitative analysis was also conducted using a smart phone and computer software,with a linearity of 0.075–0.750 ng/g,calculated LOD of 0.10 ng/g,calculated limit of quantitation of 0.33 ng/g,and good correlation of *** recoveries were found in the range of 96.4%–103.7%with a relative standard deviation of 2.5%–3.6%for intra-and *** of the results obtained by the strip test with those obtained by enzyme-linked immunosorbent assay had a good agreement in terms of ***,this strip test exhibited highly specific RAC detection without cross reactivity with related ***,the RAC–BSA conjugation via the Mannich reaction can be accepted as a one-step and easy conjugation method and applied to the competitive lateral flow strip test.

关键词： Ractopamine Conjugate of ractopamine and bovine serum albumin (RAC–BSA) Mannich reaction Lateral flow strip test Feed additive

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Production of a monoclonal antibody against oxytetracycline and its application for oxytetracycline residue detection in shrimp

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第2期15卷 165-172页

作者： Tossapon WONGTANGPRASERT Wirongrong NATAKUATHUNG Umaporn PIMPITAK Anumart BUAKEAW Tanapat PALAGA Kittinan KOMOLPIS nanthika khongchareonporn Program in Biotechnology Faculty of Science Chulalongkorn University Institute of Biotechnology and Genetic Engineering Chulalongkorn University Department of Microbiology Faculty of Science Chulalongkorn University

A novel monoclonal antibody （MAb） against oxytetracycline （OTC） was generated and characterized. The MAb was used in the development of an enzyme-linked immunosorbant assay （ELISA）-based detection system. An OTC-bovine serum albumin （BSA） conjugate was prepared and used in the immunization of mice. A conventional somatic cell fusion technique was used to generate MAb-secreting hybridomas denoted 2-4F, 7-3G, and 11-11A. An indirect competitive ELISA （icELISA） was applied to measure the sensitivity and specificity of each MAb in terms of its 50% inhibitory concentration （IC50） and percentage of cross-reactivity, respectively. MAb 2-4F exhibited the highest sensitivity, with an ICs0 of 7.01 ng/ml. This MAb showed strong cross-reactivity to rolitetracycline, but no cross- reactivity to other unrelated antibiotics. When MAb 2-4F was used to detect OTC from shrimp samples, the recoveries were in the range of 82%-118% for an intra-assay and 96%-113% for an inter-assay. The coefficients of variation of the assays were 3.9%-13.9% and 5.5%-14.9%, respectively.

关键词： Oxytetracycline Monoclonal antibody Enzyme-linked immunosorbant assay (ELISA) Shrimp

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：