检索结果-南通市图书馆

Progesterone receptor membrane component 1 as a potential prognostic biomarker for hepatocellular carcinoma

World Journal of Gastroenterology 2018年第10期24卷 1152-1166页

作者： Hung-Wen Tsai Chung-Liang Ho Shu-Wen Cheng Yih-Jyh Lin Chou-Cheng Chen Pin-nan Cheng Chia-Jui Yen Ting-Tsung Chang Po-Min Chiang Shih-Huang Chan Cheng-Hsun Ho Shu-Hui Chen Yi-Wen Wang nan-haw chow Jou-Chun Lin Institute of Clinical Medicine National Cheng Kung University Hospital College of Medicine National Cheng Kung University Department of Pathology National Cheng Kung University Hospital College of Medicine National Cheng Kung University Institute of Basic Medical Sciences College of Medicine National Cheng Kung University Center of Infectious Disease and Signaling Research College of Medicine National Cheng Kung University Department of Surgery National Cheng Kung University Hospital College of Medicine National Cheng Kung University Department of Internal Medicine National Cheng Kung University Hospital College of Medicine National Cheng Kung University Department of Statistics College of Management National Cheng Kung University Research Center of Clinical Medicine National Cheng Kung University Hospital College of Medicine National Cheng Kung University Department of Chemistry College of Sciences National Cheng Kung University

AIM To investigate the clinicopathological significance of progesterone receptor membrane component 1(PGRMC1) and PGRMC2 in hepatocellular carcinoma(HCC). METHODS We performed immunohistochemical staining to evaluate the estrogen receptor(ER), progesterone receptor(PR), PGRMC1, and PGRMC2 in a clinical cohort consisting of 89 paired HCC and non-tumor liver samples. We also analyzed HCC data(n = 373) from The Cancer Genome Atlas(TCGA). We correlated the expression status of PGRMC1 and PGRMC2 with clinicopathological indicators and the clinical outcomes of the HCC patients. We knocked down or overexpressed PGRMC1 in HCC cell lines to evaluate its biological significance in HCC cell proliferation, differentiation, migration, and invasion. RESULTS We found that few HCC cases expressed ER(5.6%) and PR(4.5%). In contrast, most HCC cases expressed PGRMC1(89.9%) and PGRMC2(100%). PGRMC1 and PGRMC2 exhibited significantly lower expression in tumor tissue than in non-tumor tissue(P < 0.001). Lower PGRMC1 expression in HCC was significantly associated with higher serum alpha-fetoprotein expression(P = 0.004), poorer tumor differentiation(P = 0.045) and liver capsule penetration(P = 0.038). Low PGRMC1 expression was an independent predictor for worse disease-free survival(P = 0.002, HR = 2.384,CI: 1.377-4.128) in our cases, as well as in the TCGA cohort(P < 0.001, HR = 2.857, CI: 1.781-4.584). The expression of PGRMC2 did not relate to patient outcome. PGRMC1 knockdown promoted a poorly differentiated phenotype and proliferation of HCC cells in vitro, while PGRMC1 overexpression caused the opposite *** PGRMC1 is a non-classical hormonal receptor that negatively regulates hepatocarcinogenesis. PGRMC1 down-regulation is associated with progression of HCC and is a poor prognostic indicator.

关键词： progesterone receptor membrane component 1 hormonal receptor proliferation hepatocellular carcinoma prognosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Carbohydrate-associated epitope-based anti-cancer drugs and vaccines

引用

Advances in Bioscience and Biotechnology 2013年第9期4卷 18-23页

作者： Gregory Lee Cheng-Yuan Huang Song-nan chow Chin-Hsiang Chien Department of Obstetrics/Gynecology National Taiwan University Hospital Taipei Chinese Taipei Institute of Biochemistry and Molecular Biology National Yang Ming University Taipei Chinese Taipei UBC Center for Reproductive Health University of British Columbia Vancouver Canada Vancouver BioTech Ltd. Vancouver Canada

RP215 is one of the three thousand monoclonal antibodies (Mabs) which were generated against the OC-3-VGH ovarian cancer cell line. RP215 was shown to react with a carbohydrate-associated epitope located specifically on glycoproteins, known as CA215, from cancer cells. Further molecular analysis by matrix adsorption laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) revealed that CA215 consists mainly of immunoglobulin super-family (IgSF) proteins, including immunoglobulins, T-cell receptors, and cell adhesion molecules, as well as several other unrelated proteins. Peptide mappings and glycoanalysis were performed with CA215 and revealed high-mannose and complex bisecting structures with terminal sialic acid in N-glycans. As many as ten O-glycans, which are structurally similar to those of mucins, were also identified. In addition, two additional O-linked glycans were exclusively detected in cancerous immunoglobulins but not in normal B cell-derived immunoglobulins. Immunizations of mice with purified CA215 resulted in the predominant generation of RP215-related Mabs, indicating the immunodominance of this carbohydrate-associated epitope. Anti-idiotype (anti-id) Mabs of RP215, which were generated in the rat, were shown to contain the internal images of the carbohydrate-associated epitope. Following immunizations of these anti-id Mabs in mice, the resulting anti-anti-id (Ab3) responses in mice were found to be immunologically similar to that of RP215. Judging from these observations, anti-id Mabs, which carry the internal image of the RP215-specific epitope, may be suitable candidates for anticancer vaccine development in humans.

关键词： Anti-Cancer Drugs Anti-Cancer Vaccines Anti-Idiotype CA215 Carbohydrate-Associated Epitope Immunodominance RP215

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物类似药证据链完备性的合成P值法评估(英文)

引用

药物分析杂志 2015年第5期35卷 832-836页

作者： Zhi-ying Pan nan Zhang Yao Yu Eric Chi Shein-chung chow Amgen Inc. Thousand Oaks California USA Duke University School of Medicine Durham North Carolina USA

最近几年,许多生物制药/生物技术公司对研发生物类似药物的兴趣越来越强。根据生物类似药物的研发需求,美国药监局(FDA)在2012年颁布了3个相关指导原则草案。FDA在这3个指导原则中表示,对于生物类似药物的注册申请的全部提交资料采用"... 详细信息

最近几年,许多生物制药/生物技术公司对研发生物类似药物的兴趣越来越强。根据生物类似药物的研发需求,美国药监局(FDA)在2012年颁布了3个相关指导原则草案。FDA在这3个指导原则中表示,对于生物类似药物的注册申请的全部提交资料采用"证据链完备性(totality-of-the-evidence)"的方法来审查,并鼓励研发企业使用"逐步递进法stepwise"来找出与原研产品相比,仿制生物制品在每一工艺步骤中的残余不确定度,进而在后续的研发步骤中设法解决这些残余不确定度。尽管要获得"具有较小不确定度的充足证据链完备性"这样的概念可以理解,但是,如何根据提交的所有注册申请信息客观地评价"证据链完备性",以及如何基于已有信息确定下一步研发步骤的程度和范围仍然是需解决的具体操作问题。本研究探索了费舍尔合成P值的概念(Fisher,1932)在等效性检验设置中的应用,更具体地说,在一个生物类似药物的临床研究中,根据"逐步递进法"和"临床证据完备性"的概念,用它来设计和分析2个生物类似药物的临床相似性。

关键词：生物相似性证据链完备性逐步法方式合成P值法 P值的权重总和法 Fisher法等效性检验

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Green Tea Polyphenols Prevent Early Vascular Aging Induced by High-Fat Diet via Promoting Autophagy in Young Adult Rats

引用

Current Medical Science 2022年第5期42卷 981-990页

作者： Xiang-tian XIAO Shui-qing HE nan-nan WU Xue-chun LIN Jing ZHAO Chong TIAN Medical College of Wuhan University of Science and Technology Wuhan430081China Department of Nutrition and Food Hygiene Hubei Key Laboratory of Food Nutrition and SafetySchool of Public HealthTongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China School of Public Health and Management Research Center for Medicine and Social DevelopmentInnovation Center for Social Risk Governance in HealthChongqing Medical UniversityChongqing400016China School of Nursing Tongji Medical CollegeHuazhong University of Science and TechnologyWuhan430030China

Objective Epidemiology studies indicate that green tea polyphenols(GTP)perform a protective effect on cardiovascular diseases,but the underlying mechanisms are *** present study aimed to investigate the effect of GTP on high-fat diets(HFD)induced-early vascular *** Six-week young adult Wistar rats were fed with standard chow or HFD in the presence and absence of GTP(200 mg/kg body weight)for 18 *** vitro experiment,human umbilical vascular endothelial cells(HUVECs)were treated with palmitic acid(PA)and *** The results showed that GTP alleviated the disorganized arterial wall and the increased intima-media thickness induced by *** addition,the vascular oxidative injury was suppressed following GTP ***,GTP elevated the ratio of LC3-II/LC3-I and suppressed expression of p62/SQSTM1,and restored SIRT3 expression in the aorta of HFD ***,in cultured HUVECs,GTP inhibited cell senescence indicated by SA-β-gal and promoted endothelial autophagy compared with the PA treatment *** activity of SIRT3 was specifically inhibited by 3-TYP,and the protective effect of GTP was consequently *** The findings indicated that GTP protected against early vascular senescence in young HFD rats via ameliorating oxidative injury and promoting autophagy which was partially regulated by the SIRT3 pathway.

关键词： high-fat diets green tea polyphenols early vascular aging autophagy Sirtuin 3

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Folic acid attenuates high-fat diet-induced steatohepatitis via deacetylase SIRT1-dependent restoration of PPARα

引用

World Journal of Gastroenterology 2020年第18期26卷 2203-2220页

作者： Feng-Zhi Xin Ze-Hua Zhao Rui-nan Zhang Qin Pan Zi-Zhen Gong Chao Sun Jian-Gao Fan Center for Fatty Liver Department of GastroenterologyXinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghai 200092China Shanghai Key Lab of Pediatric Gastroenterology and Nutrition Shanghai 200092China Shanghai Institute for Pediatric Research Shanghai Jiao Tong University School of MedicineShanghai 200092China Department of Pediatric Surgery Xinhua HospitalShanghai Jiao Tong University School of MedicineShanghai 200092China

BACKGROUND Folic acid has been shown to improve non-alcoholic steatohepatitis(NASH),but its roles in hepatic lipid metabolism,hepatic one-carbon metabolism,and gut microbiota are still *** To demonstrate the role of folic acid in lipid metabolism and gut microbiota in *** Twenty-four Sprague-Dawley rats were assigned into three groups:chow diet,high-fat diet(HFD),and HFD with folic acid *** the end of 16 wk,the liver histology,the expression of hepatic genes related to lipid metabolism,one-carbon metabolism,and gut microbiota structure analysis of fecal samples based on 16 S r RNA sequencing were measured to evaluate the effect of folic *** acid-exposed Huh7 cell line was used to evaluate the role of folic acid in hepatic lipid *** Folic acid treatment attenuated steatosis,lobular inflammation,and hepatocellular ballooning in rats with HFD-induced *** related to lipid de novo lipogenesis,β-oxidation,and lipid uptake were improvedin HFD-fed folic acid-treated ***,peroxisome proliferator-activated receptor alpha(PPARα)and silence information regulation factor 1(SIRT1)were restored by folic acid in HFD-fed rats and palmitic acid-exposed Huh7 cell *** restoration of PPARαby folic acid was blocked after transfection with SIRT1 si RNA in the Huh7 cell ***,folic acid administration ameliorated depleted hepatic one-carbon metabolism and restored the diversity of the gut microbiota in rats with HFD-induced *** Folic acid improves hepatic lipid metabolism by upregulating PPARαlevels via a SIRT1-dependent mechanism and restores hepatic one-carbon metabolism and diversity of gut microbiota,thereby attenuating HFD-induced NASH in rats.

关键词： Nonalcoholic fatty liver disease Folic acid Gut microbiota PPARα SIRT1

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

FOSSIL VERTEBRATES FROM THE MIOCENE OF NORTHERN KIANGSU

引用

古脊椎动物学报(中英文） 1964年第4期 348-354页

作者： chow MINCHEN WANG BANYUE Institute of Vertebrate Paleontology and Paleoanthropology, Academia Sinica

In this paper some vertebrate fossils collected from the Miocene at Puchen,nan-king and at Hsiachaohwan,Shihung County,both in northern Kiangsu,are described;and the age of the localities as well as that of the “Hsiachaohwan fauna”are *** Puchen faunaThe fossils were found when siliceous sands were quarryed from a small hill at Pu-chen,a town in the nanking district,some 8 kilometers on the northern bank of the

关键词：脊椎动物周明镇 FOSSIL VERTEBRATES FROM THE MIOCENE OF NORTHERN KIANGSU 中新世第三纪原大伴月

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Applying clustering coefficient to the pattern of international author collaboration in neuroimmunology and neuroinflammation

引用

Neuroimmunology and Neuroinflammation 2018年第3期5卷 20-28页

作者： Chen-Fang Hsu Tsair-Wei Chien Julie Chi chow Willy Chou Department of Paediatrics Chi-Mei Medical CenterTainan710Taiwan Research Department Chi-Mei Medical CenterTainan710Taiwan Department of Paediatrics Kaohsiung Medical UniversityKaohsiung800Taiwan Department of Physical Medicine and Rehabilitation Chi-Mei Medical CenterTainan710Taiwan Department of Recreation and Health-Care Management&Institute of Recreation Industry Management Chia Nan University of PharmacyTainan711Taiwan

Aim: To apply cluster coefficient (CC) to the pattern of international author collaborations in neuroimmunology and neuroinflammation using data from Medline and to visualize results using Google maps and social network analysis (SNA). Methods: Selecting 2799 abstracts, author names, countries, and keywords on January 22, 2018 from Medline based on keyword neuroimmunology (or neuroinflammation) within the article title since 1982, we reported following features: (1) nation distribution for the 1st author's nationality;(2) eminent journals and authors in the field of neuroimmunology and neuroinflammation;(3) notable keywords defined by authors representing both neuroimmunology and neuroinflammation;and (4) CCs in networks. We programmed Microsoft Excel VBA routines to extract data from Medline and used Google Maps and SNA Pajek software to display graphical representations with an easy-to-read feature for readers. Results: We found that: (1) the most number of papers in neuroimmunology and neuroinflammation are from the USA (902, 32.23%) and China (363, 12.97%);(2) the productive journals and authors in neuroimmunology and neuroinflammation are J Neuroinflammation and PLoS One, and Michael T. Heneka (Germany) and Richard M. Ransohoff (USA);(3) the most linked keywords are interleukin (IL), IL-1beta, and blood brain barrier;(4) author networks present higher CC than those nation networks. Conclusion: SNA provides wide and deep insight into the relationships among nations in co-author collaboration. The results can help readers in future submission to a journal in neuroimmunology and neuroinflammation.

关键词： Authorship collaboration Google Maps neuroimmunology neuroinflammation social network analysis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The most highly-cited authors who published papers on the topic of health behavior: A Bibliometric Analysis

引用

Advances in Health and Behavior 2018年第1期1卷 24-29页

作者： Chen Fang Hsu Tsair Wei Chien Julie Chi chow Willy Chou Department of Paediatrics Chi Mei medical center Tainan Taiwan Department of Paediatrics Kaohsiung Medical University Kaohsiung Taiwan Research Department Chi-Mei Medical Center Tainan Taiwan Department of physical medicine and rehabilitation Chi Mei medical center Tainan Taiwan Department of Recreation and Health-Care Management Institute of recreation Industry Management Chia Nan University of Pharmacy Tainan Taiwan

Background: Health behavior (HB) is an action taken by a person who pursues good health and prevents illness. Health behavior, thus, reflects a person’s health beliefs and attracts, particularly, on published papers in academics. However, who is the most influential author (MIA) with highly-cited papers on HB remains unknown. Objective: The purpose of this study is to apply the authorship-weighted scheme (AWS) developed by authors to select the MIA on HB using the visual displays on Google Maps. Methods: We obtained 1,116 abstracts published between 2012 and 2016 from Medline based on the keywords of (health [Title]) and (behavior [Title] or behavior [Title]) on September 22, 2018. The author names, countries/areas, and Pubmed paper IDs were recorded. The AWS was applied to (1) select the most productive authors (MPA) using social network analysis (SNA);(2) discover the MIA using h-indexes and author impact factors (AIF) dispersed on Google Maps, and (3) display the countries/areas distributed for the x-index in geography. Pajek software was performed to determine the partition categories of clusters. Results: We found that the MPA and MIA are Matthew K Nock (US) and Erika A Waters (US) for the MPA and MIA, respectively. All visual representations that are the form of a dashboard can be easily displayed on Google Maps. The most influential countries are the US (=19.03) and Australia (=6.46) with the highest x-indexes. Readers are suggested to manipulate them on their own on Google Maps. Conclusion: Many individual researchers achievements (IRA) were determined using h-index, AIF, x-index, or other bibliometric indices without quantifying author contributions. We demonstrated visualized representations on Google Maps using the AWS developed by authors to measure authors influences in a specific discipline. The research approach using the AWS to quantify the authors contributions can be applied to measure IRA in the future.

关键词： authorship-weighted scheme most productive author most influential author Google Maps social network analysis health behavior

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Edible Bird's Nest Attenuates Menopause-Related Bone Degeneration in Rats via Increasing Bone Estrogen-Receptor Expression

引用

Chinese Journal of Integrative Medicine 2021年第4期27卷 280-285页

作者： HOU Zhi-ping TANG Shi-ying Jl Hai-ru HE Pei-yuan LI Yu-hong DONG Xian-ling DU Meng-nan Ismail Maznah HE Wen-jing Department of Pathology Chengde Medical UniversityChengdeHebei Province(067000)China Department of Gastroenterology The Affiliated Hospital of Chengde Medical UniversityChengdeHebei Province(067000)China Department of Pathology The Affiliated Hospital of Hebei University BaodingHebei Province(0671000)China Laboratory of Molecular Biomedicine Institute of BioscienceUniversiti Putra MalaysiaSerdangSelangor(43400)Malaysia Department of Gastroscopy Kuancheng Manchu Autonomous County HospitalChengdeHebei Province(067000)China

Objective:To investigate the mechanistic basis for the attenuation of bone degeneration by edible bird's nest(EBN)in ovariectomized ***:Forty-two female Sprage-Dawley rats were randomized into 7 groups(6 in each group).The ovariectomized(OVX)and OVX+6%,3%,and 1.5%EBN and OVX+estrogen groups were given standard rat chow alone,standard rat chow+6%,3%,and 1.5%EBN,or standard rat chow+estrogen therapy(0.2mg/kg per day),*** sham-operation group was surgically opened without removing the *** control group did not have any surgical *** 12 weeks of intervention,blood samples were taken for serum estrogen,osteocalcin,and osteoprotegerin,as well as the measurement of magnesium,calcium abd zinc *** femurs were removed from the surrounding muscles to measure bone mass density using the X-ray edge detection technique,then collected for histology and estrogen receptor(ER)***:Ovariectomy altered serum estrogen levels resulting in increased food intake and weight gain,while estrogen and EBN supplementation attenuated these *** also reduced bone ER expression and density,and the production of osteopcalcin and osteorotegerin,which are important pro-osteoplastic hormones that promote bone mineraliztion and ***,estrogen and EBN increased serum estrogen levels leading to increased bone ER expression,pro-osteoplastic hormone production and bone density(all P<0.05).Conclusion:EBN could be used as a safe alternative to hormone replacement therapys for managing menopausal complications like bone degeneration.

关键词： edible bird's nest menopause ovariectomy rats bone osteoporosis

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Detecting Dengue Fever in Children: Using Sequencing Symptom Patterns for An Online Assessment Approach

引用

Advances in Health and Behavior 2018年第1期1卷 12-16页

作者： Tsair-Wei Chien Julie Chi chow Yu Chang Willy Chou Medical Research Department Chi-Mei Medical Center Tainan Taiwan Department of Paediatrics Chi-Mei medical center Tainan Taiwan National Taiwan University School of Medicine Taiwan Department of Sports Management College of Leisure and Recreation Management Chia Nan University of Pharmacy and Science Tainan Taiwan Ncphrology Department Chi-Mei Medical Center Tainan Taiwan

Background: Dengue fever (DF) is an important health problem in Asia. We examined it using its clinical symptoms to predict DF. Methods: We extracted statistically significant features from 17 DF-related clinical symptoms in 177 pediatric patients (69 diagnosed with DF) using the unweighted summation score and the non-parametric HT person fit statistic, which jointly combine the weighted score (yielded by logistic regression) to predict DF risk. Results: Six symptoms (Family History, Fever 39C, Skin Rash, Petechiae, Abdominal Pain, and Weakness) significantly predicted DF. When a cutoff point of 1.03 (p = 0.26) suggested combining the weighted score and the HT coefficient, the sensitivity was 0.91 and the specificity was 0.76. The area under the ROC curve was 0.88, which was a better predictor: specificity was 5.56% higher than for the traditional logistic regression. Conclusions: Six simple symptoms analyzed using logistic regression were useful and valid for early detection of DF risk in children. A better predictive specificity increased after combining the non-parametric HT coefficient to the weighted regression score. A self-assessment using patient smart phones is available to discriminate DF and may eliminate the need for a costly and time-consuming dengue laboratory test.

关键词： dengue fever HT person mapping statistic logistic regression score summation receiver operating characteristic curve

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：