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C57BL/6新生乳鼠肠神经胶质细胞的提取与培养

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中国组织工程研究 2025年第31期29卷 6656-6660页

作者：赵楠丁勇修航刘鹏飞梁国刚大连医科大学附属第一医院中西医结合临床国家重点学科实验室辽宁省大连市116000 大连医科大学附属第一医院普外三科辽宁省大连市116000

背景:炎症性肠病的发病机制涉及炎症、免疫激活、内脏高敏感、肠道菌群失调等,其中炎症促使免疫细胞释放炎症递质,损伤肠神经系统。肠神经胶质细胞是肠神经系统的重要组成部分,是研究肠道神经炎症的良好细胞。原代肠神经胶质细胞对于探... 详细信息

背景:炎症性肠病的发病机制涉及炎症、免疫激活、内脏高敏感、肠道菌群失调等,其中炎症促使免疫细胞释放炎症递质,损伤肠神经系统。肠神经胶质细胞是肠神经系统的重要组成部分,是研究肠道神经炎症的良好细胞。原代肠神经胶质细胞对于探索肠神经系统疾病的细胞疗法具有至关重要的作用,而目前获得该细胞的方法大多较繁琐,因此找到一种便捷、快速提取该细胞的方法十分关键。目的:建立优化分离培养及鉴定小鼠肠神经胶质细胞的方法。方法:通过过量吸入异氟烷处死0-7 d龄C57BL/6乳鼠,将其浸泡于体积分数75%乙醇消毒后腹正中线开腹取出十二指肠(幽门下1 cm至屈氏韧带上1 cm),用1 mL注射器充满DPBS反复冲洗肠内容物至肠道半透明,剥离肠系膜及血管。将十二指肠剪至1 mm大小,放入含0.25%EDTA胰酶中消化20 min后加入等量DMEM/F12完全培养基终止消化,用100μm细胞滤器过滤后离心,加入1 mL DMEM/F12完全培养基重悬后培养细胞,细胞贴壁生长密度至80%时消化传代。细胞培养至第3代时用标记肠神经胶质细胞的胶质纤维酸性蛋白进行免疫荧光鉴定。结果与结论:分离培养的细胞胶体饱满,突起向外延展,可以传代,胶质纤维酸性蛋白染色阳性。该方法可成功分离培养肠神经胶质细胞,且操作简便,为肠神经系统的病理生理学研究提供稳定的模型。

关键词：肠神经胶质细胞乳鼠细胞培养炎症性肠病肠神经系统胶质纤维酸性蛋白工程化细胞

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The dynamic response and damage models of rebar reinforced polymer slabs subjected to contact and near-field explosions

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Defence Technology（防务技术） 2023年第10期28卷 330-342页

作者： Hao-nan zhao Hong-yuan Fang Xiao-hua zhao Yellow River Laboratory Zhengzhou UniversityZhengzhou450001China

Non aqueous reactive polymer materials produced by the reaction of isocyanate and polyol have been widely used in infrastructure construction,which may be subjected to explosion loads during complex service *** blast response of composite materials is a crucial aspect for applications in engineering structures potentially subjected to extreme *** this work,damage caused to rebar reinforced polymer slabs by surface explosive charges was studied experimentally and numerically.A total of 6 field tests were carried out to investigate the performances of the failure modes of rebar reinforced polymer slabs under contact and near-field *** influence of explosive quantity(10-40 g)and stand-off distances(0-20 cm)at the damage modes were *** results show that the failure modes of rebar reinforced polymer slabs under near-field explosion mainly were bending and surface spalling,while under the impact of contact explosion,the failure modes were craters of the top surface,spalling of the bottom surface,and middle ***,a detailed fully coupled model was developed and validated with the test *** influences of explosive quantity and slab thickness on rebar reinforced polymer slabs under contact explosion were *** on this,the calculation formula between breach diameter,explosive quantity,and slab thickness is fitted.

关键词： Reinforced polymer slab Numerical investigations Empirical prediction Damage models

Fluid Resistance Characteristics Research of nanowire Rotation under a Magnetic Field

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Journal of Thermal Science 2015年第1期24卷 73-81页

作者： Lixin Yang nan zhao Li Jia Institute of Thermal Engineering School of Mechanical Electronic and Control Engineering Beijing Jiaotong University Beijing Key Laboratory of flow and heat transfer of phase changing in micro and small scale

In this study, a visualization-based experiment was performed to measure the motion of the nanowire under a magnetic field. A simulation method based on a multiple reference flame model （MRF model） was used to calcu- late fluid torque. Here, it was validated with the experimental data and theoretical results. Fluid torque of steady rotated nanowire was simulated and compared using experiment and theoretical models. The unsteady rotated condition was studied using transient simulation to compare with theory and the results showed that the accelera- tion of nanowire did not affect the flow field, indicating that the theoretical models based on the steady condition were still valid. The influence of solid walls on nanowire rotation was also studied here. The results showed that if the nanowire was placed close to the wall, the viscous force of wall would increase the velocity gradient around the nanowire, causing higher torque predictions. The fluid torque decreased quickly when the vertical distance between nanowire and wall exceeded 5 times the diameter of the wire.

关键词： nanowire rotation simulation fluid torque wall effect

Hybrid opto-mechanical systems with nitrogen-vacancy centers

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Science China(Physics,Mechanics & Astronomy) 2015年第5期58卷 29-40页

作者： YIN ZhangQi zhao nan LI TongCang Center for Quantum Information Institute for Interdisciplinary Information Sciences Tsinghua University Beijing Computational Science Research Center Synergetic Innovation Center of Quantum Information and Quantum Physics University of Science and Technology of China Department of Physics and Astronomy and School of Electrical and Computer Engineering Purdue University Birck nanotechnology Center Purdue University

In this review, we briefly review recent works on hybrid （nano） and diamond nitrogen-vacancy （NV） centers. We also review opto-mechanical systems that contain both mechanical oscillators two different types of mechanical oscillators. The first one is a clamped mechanical oscillator, such as a cantilever, with a fixed frequency. The second one is an optically trapped nano-diamond with a built-in nitrogen-vacancy center. By coupling mechanical resonators with electron spins, we can use the spins to control the motion of mechanical oscillators. For the first setup, we discuss two different coupling mechanisms, which are magnetic coupling and strain induced coupling. We summarize their applications such as cooling the mechanical oscillator, generating entanglements between NV centers, squeezing spin ensembles etc. For the second setup, we discuss how to generate quantum superposition states with magnetic coupling, and realize matter wave interferometer. We will also review its applications as ultra-sensitive mass spectrometer. Finally, we discuss new coupling mechanisms and applications of the field.

关键词： opto-mechanics nitrogen-vacancy center hybrid system

Heterogeneous reaction mechanism of gaseous HNO3 with solid NaCI： a density functional theory study

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Frontiers of Environmental Science & Engineering 2016年第5期10卷 49-58页

作者： nan zhao Qingzhu ZHANG Wenxing WANG Environment Research Institute Shandong University Jinan 250100 China

Sea salt particles containing NaCl are among the most abundant particulate masses in coastal atmosphere. Reactions involving sea salt particles potentially generate Cl radicals, which are released into coastal atmosphere. Cl radicals play an important role in the nitrogen and O3 cycles, sulfitr chemistry and particle formation in the troposphere of the polluted coastal regions. This paper aimed at .the heterogeneous reaction between gaseous HNO3 .and solid NaCl. The ***.m was *** density functional theory （DFT）. The results imply that water molecules induce the surface reconstruction, which is essential for the heterogeneous reaction. The surface reconstruction on the defective （710） surface has a barrier of 10.24 ***-1 and is endothermic by 9.69 ***-1, whereas the reconstruction on the clean （100） surface has a barrier of 18.46 ***-1 and isendothermic by 12.96 ***-1. The surface reconstruction involved in water-adsorbed （710） surface is more energetically favorable. In comparison, water molecules adsorbed on NaCl （100） surface likely undergo water diffusion or desorption. Further, it reveals that the coordination number of the Cl-out is reduced after the surface reconstruction, which assists Clout to accept the proton from HNO3. HCl is released from heterogeneous reactions between gaseous HNO3 and solid NaCl and can react with OH free radicals to produce atomic Cl radicals. The results will offer further insights into the impact of gaseous HNO3 on the air quality of the coastal areas.

关键词： Seasalt particles NaCl HNO3 Heterogeneous reaction Reaction mechanism Density functional theory

Quantum noise of a harmonic oscillator under classical feedback control

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Chinese Physics B 2020年第9期29卷 212-216页

作者： Feng Tang nan zhao Beijing Computational Science Research Center Beijing 100193China

Quantum sensing has been receiving researcher's attention these years due to its ultrahigh sensitivity and precision. However, the bandwidth of the sensors may be low, thus limiting the scope of their practical applications. The low-bandwidth problem is conquered by feedback control methods, which are widely utilized in classic control fields. Based on a quantum harmonic oscillator model operating near the resonant point, the bandwidth and sensitivity of the quantum sensor are analyzed. The results give two important conclusions: (a) the bandwidth and sensitivity are two incompatible performance parameters of the sensor, so there must be a trade-off between bandwidth and sensitivity in practical applications;(b) the quantum white noise affects the signal to be detected in a non-white form due to the feedback control.

关键词： quantum sensing feedback sensitivity bandwidth

Energy-efficient UAV-NOMA aided wireless coverage with massive connections

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Science China(Information Sciences) 2023年第12期66卷 194-208页

作者： Yuqiao TONG Min SHENG Junyu LIU nan zhao School of Information and Communication Engineering Dalian University of Technology State Key Laboratory of Integrated Services Networks Xidian University

In this paper, we propose an unmanned aerial vehicle(UAV)-assisted wireless coverage scheme,wherein UAVs help to serve the cell-edge users for ground base stations based on non-orthogonal multiple access(NOMA). The combined benefits of wide coverage and massive connections enable UAV-NOMA-aided wireless coverage to effectively meet the requirements of the Internet of Everything. Meanwhile, energy efficiency(EE) is maximized via resource allocation to address the issue of the power supply bottleneck of UAVs. The non-convex optimization problem is transformed into two convex subproblems of trajectory optimization and power allocation, and an iterative alternating optimization algorithm is proposed to address it effectively. Moreover, we further considered the energy consumption of the ground base stations, effectively minimizing it by optimizing precoding while guaranteeing average throughput. Numerical results verify the effectiveness of the proposed UAV-aided wireless coverage scheme and demonstrate the EE improvement.

关键词： energy efficiency non-orthogonal multiple access trajectory optimization unmanned aerial vehicle wireless coverage

One-pot synthesis of Cu-Ce co-doped SAPO-5/34 hybrid crystal structure catalysts for NH_(3)-SCR reaction with SO_(2) resistance

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Journal of Rare Earths 2021年第10期39卷 1217-1223,I0002页

作者： Yuanyuan Ma Zhifang Li nan zhao Yanlong Teng College of Chemistry and Chemical Engineering Qiqihar UniversityQiqihar 161006China College of Materials Science and Engineering Qiqihar UniversityQiqihar 161006China Analyzing&Testing Center Qiqihar UniversityQiqihar 161006China

SAPO-34,SAPO-5/34 based catalysts doped with Cu,Ce as active components were synthesized via a one-pot hydrothermal method by using different amounts of additive(a-cellulose),and their catalytic activities were measured for selective catalytic reduction(SCR) of NO with *** synthesized Cu-Ce co-doped products switch from cubic SAPO-34,to flower-like aggregated SAPO-5/34,hybrid crystal SAPO-5/34,and finally to spherical aggregated SAPO-34 with the increase of α-cellulose *** Cu-Ce co-doped SAPO-5/34 hybrid crystal structure catalysts with 0.75 mol ratios of C/P(Cu-Ce/SP-0.75)exhibit excellent NH_(3)-SCR activity with higher than 90% NOx conversion in the temperature range of 180-450℃,at WHSV of 20000 mL/(g·h).Furthermore,the catalyst displays outstanding sulfur resistance and NOX conversion maintains above 90% at 200-450℃ after adding 100 ppm of SO_(2).The characteristic results suggest that the high deNOX performance of Cu-Ce/SP-0.75 is due to the enhanced accessibility,abundant activity species,excellent redox property and high adsorptive and activated capacity for NH_(3).

关键词： SAPO-5/34 catalysts Selective catalytic reduction Cu-Ce co-modified One-pot synthesis Good resistance to SO_(2) Rare earths

Fluorescent Silk Obtained by Feeding Silkworms with Fluorescent Materials

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Chinese Journal of Chemistry 2023年第16期41卷 2035-2046页

作者： zhao-Fan Wu zhao-nan Sun Huan-Ming Xiong Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials Fudan UniversityShanghai 200438China

Comprehensive Summary Fluorescent silk has potential application in many fields such as bioimaging,tissue engineering scaffolds,luminescent marks,and dazzling *** the methods to endow natural silk with fluorescent properties,feeding silkworms with fluorescent additives is facile,low-cost and environment friendly,which has the prospect of large-scale *** this paper,we reviewed the research progress for this aim in the past ten years,and summarized the unified characteristics for the substances that can enter the silk gland by digestive tract of *** advantages and disadvantages of various fluorescent materials for this application are compared in *** the future research directions are suggested to overcome the shortcomings of the present research.

关键词： Fluorescent silk Fluorescent materials Feeding silkworms Carbon dots nanoparticles nanotechnology Multi-color emission Bioimaging