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The role of extracellular vesicles in animal reproduction and diseases

Journal of Animal Science and Biotechnology 2022年第6期13卷 1634-1656页

作者： Sangiliyandi Gurunathan Min-Hee Kang Hyuk Song nam hyung kim Jin-Hoi kim Department of Stem Cell and Regenerative Biotechnology Konkuk UniversitySeoul 05029Korea Guangdong Provincial Key Laboratory of Large Animal models for Biomedicine Wuyi UniversityJiangmen 529020China

Extracellular vesicles(EVs)are nanosized membrane-enclosed compartments that serve as messengers in cell-to-cell communication,both in normal physiology and in pathological *** can transfer functional proteins and genetic information to alter the phenotype and function of recipient cells,which undergo different changes that positively affect their structural and functional *** fluids are enriched with several subpopulations of EVs,including exosomes,microvesicles(MVs),and apoptotic bodies carrying several cargoes,such as lipids,proteins,and nucleic *** associated with the reproductive system are actively involved in the regulation of different physiological events,including gamete maturation,fertilization,and embryo and fetal *** can influence follicle development,oocyte maturation,embryo production,and endometrial-conceptus *** loaded with cargoes are used to diagnose various diseases,including pregnancy disorders;however,these are dependent on the type of cell of origin and pathological ***-derived microRNAs(miRNAs)and proteins in the placenta regulate inflammatory responses and trophoblast invasion through intercellular delivery in the placental *** review presents evidence regarding the types of extracellular vesicles,and general aspects of isolation,purification,and characterization of EVs,particularly from various types of ***,we discuss EVs as mediators and messengers in reproductive biology,the effects of EVs on placentation and pregnancy disorders,the role of EVs in animal reproduction,in the male reproductive system,and mother and embryo *** addition,we emphasize the role of microRNAs in embryo implantation and the role of EVs in reproductive and therapeutic ***,we discuss the future perspectives of EVs in reproductive biology.

关键词： Embryos Extracellular vesicles Implantation MicroRNA Pregnancy disorders

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Interstitial tissue-specific gene expression in mouse testis by intra-tunica albuguineal injection of recombinant baculovirus

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Asian Journal of Andrology 2009年第3期11卷 342-350页

作者： Hyun Jung Park Won Young Lee Jin Hoi kim Jae Hwan kim Hun Jong Jung nam hyung kim Bo Kyung kim Hyuk Song Department of Animal Science College of Natural Science Konkuk University Chung-ju 380-701 Korea CHA Stem Cell Institute Graduate School of Life Science and Biotechnology Pochon CHA University Seoul 135-907 Korea Department of Animal Biotechnology College of Animal Biotechnology Konkuk University Seoul 143-701 Korea Occupation and Environment Department Medical School Konkuk University Chung-ju 380-704 Korea Department of Animal Science College of Agriculture Chungbuk National University Choung-ju Chung-buk 361-763 Korea Department of Physiology Medical School Konkuk University Chung-ju 380-701 Korea

The purpose of this study is to establish a gene delivery system for interstitial tissue-specific protein expression in mice testes using modified recombinant baculovirus. Green fluorescent protein （GFP）-expressing recombinant baculovirus （GFP-baculovirus）, in which the insect cell-specific polyhedron promoter was replaced by the cytomegalovirus （CMV）-IE promoter, was used to transfect testicular cells in vitro, and for intra-tunica albuguineal injection of the interstitial tissue of the testis. GFP expression was monitored in frozen testes sections by fluorescence microscopy. Expression of GFP in testicular tissues was also assessed by reverse transcription polymerase chain reaction （RT-PCR）, and protein expression was assessed by Western blot. Testicular cells in vitro were infected efficiently by modified recombinant GFP-baculovirus. lntra-tunica albuguineal injection of GFP- baculovirus into the mouse testis resulted in a high level of GFP expression in the interstitial tissues. RT-PCR analysis clearly showed GFP gene expression in the testis, particularly interstitial tissues. Intra-tunica albuguineal injection of a modified baculovirus that encoded recombinant rat insulin-like growth factor binding protein （IGFBP）-5 resulted in an increase in IGFBP-5 in testis and semen. In conclusion, we have developed an efficient delivery system for gene expression in vivo in testicular cells, particularly cells of the interstitial tissue using intratunica albuguineal injection of a modified recombinant baculovirus. This method will be particularly relevant for application that requires gene delivery and protein expression in the testicular cells of the outer seminiferous tubule of the testis.

关键词： intra-tunica albuguineal injection recombinant baculovirus testis gene delivery

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Identifying MicroRNA and mRNA Expression Profiles in Embryonic Stem Cells Derived from Parthenogenetic,Androgenetic and Fertilized Blastocysts

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Journal of Genetics and Genomics 2013年第4期40卷 189-200页

作者： Xiang-Shun Cui Xing-Hui Shen Shao-Chen Sun Sun-Wha Cho Young-Tae Heo Yong-Kook Kang Teruhiko Wakayama nam-hyung kim Department of Animal Science Chungbuk National UniversityGaeshin-dongCheongjuChungbuk 361-763South Korea Department of Histology and Embryology Harbin Medical UniversityHarbin 150081China College of Animal Science and Technology Nanjing Agricultural UniversityNanjing 210095China Laboratory of Development and Differentiation Korea Research Institute of Bioscience and BiotechnologyDaejeon 305-806South Korea Center for Developmental Biology RIKEN KobeKobe 650-0047Japan

MicroRNAs（miRNAs） are a class of highly conserved small non-coding RNA molecules that play a pivotal role in several cellular *** this study,miRNA and messenger RNA（mRNA） profiles were examined by Illumina microarray in mouse embryonic stem cells（ESCs） derived from parthenogenetic,androgenetic,and fertilized *** global analysis of miRNA-mRNA target pairs provided insight into the role of miRNAs in gene *** showed that a total of 125 miRNAs and 2394 mRNAs were differentially expressed between androgenetic ESCs（aESCs） and fertilized ESCs（fESCs）,a total of 42 miRNAs and 87 mRNAs were differentially expressed between parthenogenetic ESCs（pESCs） and fESCs,and a total of 99 miRNAs and 1788 mRNAs were differentially expressed between aESCs and *** addition,a total of 575,5 and 376 miRNA-mRNA target pairs were observed in aESCs ***,pESCs ***,and aESCs ***,***,15 known imprinted genes and 16 putative uniparentally expressed miRNAs with high expression levels were confirmed by both microarray and real-time ***,transfection of miRNA inhibitors was performed to validate the regulatory relationship between putative maternally expressed miRNAs and target mRNAs. Inhibition of miR-880 increased the expression of Peg3,Dyrklb,and Prrg2 mRNA,inhibition of miR-363 increased the expression of Nfat5 and Soatl mRNA,and inhibition of miR-883b-5p increased Nfat5,Tacstd2,and Ppapdc1 *** results warrant a functional study to fully understand the underlying regulation of genomic imprinting in early embryo development.

关键词： Embryonic stem cell Parthenogenetic Androgenetic Fertilized Microarray miRNA-mRNA network

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MICRORNA 127 AND 136 IN MOUSE FERTILIZED,PARTHENOGENIC,ANDROGENIC AND SCNT CLONED EMBRYOS

MICRORNA 127 AND 136 IN MOUSE FERTILIZED,PARTHENOGENIC,ANDRO...

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The 5th Annual Conference of the Asian Reproductive Biotechnology Society

作者： Jae-Dal Lee nam-hyung kim Department of Pet Care Hyejeon CollegeHongsung-gunChungnam350-702Korea Department of Animal Science Chungbuk National UniversityChungbuk 361-763Korea

＜正＞MicroRNAs（miRNAs）are small non-coding RNAs approximately 22-25 nucleotides in length that function in regulating gene expression at the post-transcriptional *** evidence suggests that miRNAs play a very import...

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Production of Transgenic Korean Native Cattle Expressing Enhanced Green Fluorescent Protein Using a FIV-Based Lentiviral Vector Injected into MII Oocytes

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Journal of Genetics and Genomics 2013年第1期40卷 37-43页

作者： Yong-Nan Xu Sang-Jun Uhm Bon-Chul Koo Mo-Sun Kwon Ji-Yeol Roh Jung-Seok Yang Hyun-Yong Choi Young-Tae Heo Xiang-Shun Cui Joon-Ho Yoon Dae-Hwan Ko Teoan kim nam-hyung kim Department of Animal Sciences Chungbuk National University361-763Republic of Korea Department of Animal Science and Biotechnology Sangji Youngseo College220-713Republic of Korea Department of Physiology Catholic University of Daegu School of Medicine712-702Republic of Korea Department of Animal Resources Community KongJu National University340-702Republic of Korea

The potential benefits of generating and using transgenic cattle range from improvements in agriculture to the production of large quantities of pharmaceutically relevant proteins. Previous studies have attempted to produce transgenic cattle and other livestock by pronuclear injection and somatic cell nuclear transfer, but these approaches have been largely ineffective; however, a third approach, lentivirus-mediated transgenesis, has successfully produced transgenic livestock. In this study, we generated transgenic （TG） Korean native cattle using perivitelline space injection of viral vectors, which expressed enhanced green fluorescent protein （EGFP） systemically. Two different types of lentiviral vectors derived from feline immunodeficiency virus （FIV） and human immunodeficiency virus （HIV） carrying EGFP were injected into the perivitelline space of MII oocytes. EGFP expression at 8-cell stage was significantly higher in the FIV group compared to the HIV group （47.5% ± 2.2% v.s. 22.9% 4± 2.9%）. Eight-cell embryos that expressed EGFP were cultured into blastocysts and then transferred into 40 heifers. Ten heifers were successfully impregnated and delivered 10 healthy calves. All of these calves expressed EGFP as detected by in vivo imaging, PCR and Southern blotting. In addition, we established an EGFP-expressing cell line from TG calves, which was followed by nuclear transfer （NT）. Recloned 8-cell embryos also expressed EGFP, and there were no differences in the rates of fusion, cleavage and development between cells derived from TG and non-TG calves, which were subsequently used for NT. These results illustrate that FIV-based lentiviruses are useful for the production of TG cattle. Moreover, our established EGFP cell line can be used for additional studies that involve induced pluripotent stem cells.

关键词： Transgenic cattle Lentiviral vector Perivitelline space injection Enhanced green fluorescent protein

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IN VITRO DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER EMBRYOS TREATED WITH FLAVONOID

IN VITRO DEVELOPMENT OF BOVINE SOMATIC CELL NUCLEAR TRANSFER...

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The 5th Annual Conference of the Asian Reproductive Biotechnology Society

作者： Cheol Tae JIN Eun Young kim nam hyung kim Se Pill PARK Cheju National University

＜正＞The success of somatic cell cloning gives promise to applications such as species preservation,cell therapy and production of somatic cell nuclear transfer（SCNT）embryonic stem *** improve the in vitro developm... 详细信息

关键词： bovine embryo Flavonoid Somatic cell nuclear transfer (SCNT)

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Analysis of proteomic profiling of mouse embryonic stem cells derived from fertilized, parthenogenetic and androgenetic blastocysts

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Stem Cell Discovery 2011年第1期1卷 1-15页

作者： Xiang-Shun Cui Xing-Hui Shen Chang-Kwon Lee Yong-Kook Kang Teruhiko Wakayama nam-hyung kim 不详

Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of preimplantation embryos. ESCs exhibit true pluripotency, i.e., the ability to differentiate into cells of all three germ layers in the developing embryo. We used 2-DE MALDI-TOF/TOF to identify differentially expressed proteins among three types of mouse embryonic stem cells (ESCs) derived from ferti-lized, parthenogenetic, and androgenetic (fESC, pESC and aESC, respectively) blastocysts. We detected more than 800 proteins on silver- stained gels of whole protein extracts from each type of ESC. Of these, 52 differentially expressed proteins were identified by the MALDI–TOF/TOF analyzer, including 32 (fESCs vs. pESCs), 28 (fESCs vs. aESCs) and 17 (pESCs vs. aESCs) prominent proteins with significantly higher or lower expression relative to the comparison cells. Among the 32 proteins from fESCs, 12 were significantly increased in expression and 20 were reduced in expression in fESCs com-pared with pESCs. Similarly, 10 of 28 and 8 of 17 proteins were more highly expressed in fESCs and pESCs compared with aESCs, respectively. In contrast, 18 of 28 and 9 of 17 proteins were reduced in expression in fESCs and pESCs compared with aESCs, respectively. Of the eight protein candidates in fESCs, four were in-creased and four were decreased in expression relative to both pESCs and aESCs in the 2-DE analysis. Differential expression of these pro-teins were confirmed by mRNA expression analysis using real time RT-PCR. For three pro-teins, ANXA5, CLIC1 and SRM, Western blot analysis corroborated the expression patterns indicated by the 2-DE results. ANXA5 and CLIC1 were increased in expression and SRM was de-creased in expression in fESCs compared with both pESCs and aESCs. The differentially ex-pressed proteins identified in the present study warrant further investigation towards the goal of their potential application in ESC therapy.

关键词： Protein Profiling Embryonic Stem Cell Parthenogenote Androgenote Fertilization

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Generation of pigs with humanized type Ⅱ collagen by precise human COL2A1 gene knock-in

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Journal of Genetics and Genomics 2023年第3期50卷 212-215页

作者： Ting Lan Yuling Zheng Yangyang Suo Yuhui Wei Hui Shi Quanmei Yan Zhenpeng Zhuang Huangyao Chen Quanjun Zhang Nana Fan Yu Zhao Zhen Ouyang Chengdan Lai Zhaoming Liu Jizeng Zhou Chengcheng Tang nam-hyung kim Qingjian Zou Xiaomin Wang Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesWuyi UniversityJiangmenGuangdong 529020China University of Chinese Academy of Sciences Beijing 100049China CAS Key Laboratory of Regenerative Biology Guangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineSouth China Institute for Stem Cell Biology and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhouGuangdong 510530China Joint School of Life Science Guangzhou Institutes of Biomedicine and HealthChinese Academy of SciencesGuangzhou Medical UniversityGuangzhouGuangdong 510530China The School of Biomedical and Pharmaceutical Sciences Guangdong University of TechnologyGuangzhouGuangdong 510006China School of Life Sciences Westlake UniversityHangzhouZhejiang 310024China Institute of Biology Westlake Institute for Advanced StudyHangzhouZhejiang 310024China

Osteoarthritis(OA)is the most common chronic disease,characterized by progressive cartilage breakdown,subchondral bone sclerosis,and aberrant bone outgrowth(Yucesoy et al.,2015;Hussain et al.,2016).OA is one of the leading causes of cartilage *** with severe cartilage damage require transplantation of articular cartilage to improve their quality of *** Ⅱ collagen is a major component of articular cartilage and intervertebral discs and plays an important role in the structure and strength of connective tissues that support muscles and joints(Byers,1994).

关键词： damage cartilage

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存储器技术的未来

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世界电子元器件 2002年第10期 12-13页

作者： JIM CANTOR nam hyung kim iSuppli公司

便宜的DRAM仍占优势,但稳定的技术进步已经开拓了许多新的方向存储器技术是一种不断进步的技术,每一种新技术的出现都会使某种现存的技术走进历史,这是因为开发新技术的初衷就是为了消除或减轻某种特定存储器产品的不足之处。

关键词：存储器 OUM PFRAM 闪存非易性存储技术非易性存储器

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Toxic effects of atrazine on porcine oocytes and possible mechanisms of action

Toxic effects of atrazine on porcine oocytes and possible me...

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中国生理学会生殖科学专业委员会—中国动物学会生殖生物学分会第二次联合学术年会暨“生殖科学专业委员会第二次学术交流会”和“生殖生物学分会第十六次学术年会”

作者： Bao Yuan Shuang Liang Yong-Xun Jin Ming-Jun Zhang Jia-Bao Zhang nam-hyung kim Department of Laboratory Animals College of Animal SciencesJilin University

Because atrazine is a widely used herbicide,its adverse effects on the reproductive system have been extensively *** this study,we investigated the effects of atrazine exposure on porcine oocyte maturation and the possible *** results showed that the rates of oocyte maturation significantly decreased after treatment with 200μM atrazine in ***

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