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Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces <i>in vitro</i>

Journal of Biomedical Science and Engineering 2013年第3期6卷 273-276页

作者： nahoko miyake Tadashi Miura Toru Sato Masao Yoshinari Department of Crown & Bridge Prosthodontics Tokyo Dental College Chiba Japan Division of Oral Implants Research Oral Health Science Center Tokyo Dental College Chiba Japan Division of Prosthodontics Department of Clinical Oral Health Science Tokyo Dental College Tokyo Japan

We previously studied the mechanism underlying the adsorption of oral bacteria on the surfaces of dental prosthetic materials such as ceramics and resins in vitro. The aim of the present study was to examine bovine serum albumin (BSA) adsorption on crown composite resin surfaces by means of zeta potential. We measured the zeta potentials of resins alone, BSA alone, and resins after BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under continuous rotation (6 rpm). The zeta potentials of both resins and BSA were negative, with BSA itself less negative than the resins themselves as an absolute value (p < 0.0001). The zeta potentials of seven resin surfaces after BSA adsorption were significantly less negative than were those of the resins without BSA adsorption (p < 0.0001). Eight resins were divided into two classes based on the size of the surface potential difference between each resin and the BSA. The difference in surface potential between the resins and the BSA were small, leading to the theory that particles with identical charges repulse each other, and the amounts of adsorbed BSA on these resins might be less. On the other, when the differences between the other resins and BSA are large, so that the repulsive force between two nonidentical particles becomes zero and an attractive force might be generated, then more BSA might be adsorbed on those resins. Therefore, the zeta potentials were affected by BSA adsorption and became less negative. These results suggested that electrostatic interactions play an important role in the adsorption of BSA on resin surfaces.

关键词： Zeta Potential Crown Composite Resins Bovine Serum Albumin Adsorption Electrostatic Interactions

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Potential Antimicrobial Effects of Gatifloxacin on Periodontopathic Bacteria in Vitro

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Journal of Biomedical Science and Engineering 2016年第7期9卷 354-359页

作者： Tadashi Miura Koji Tanabe Eri Tsukagoshi Koichi Kida Yasuhiko Shizawa nahoko miyake Masataka Kasahara Masao Yoshinari Division of Oral Implants Research Oral Health Science Center Tokyo Dental College Tokyo Japan Department of Pharmacology Tokyo Dental College Tokyo Japan Department of Oral and Maxillofacial Implantology Tokyo Dental College Tokyo Japan Department of Liberal Arts College of Bioresource Sciences Nihon University Kanagawa Japan Department of Removable Partial Prosthodontics Tokyo Dental College Tokyo Japan

The aim of this study was to identify the potential antibacterial effects of gatifloxacin on periodontal pathogens including Aggregatibacter actinomycetemcomitans, Porphyromonas gingi-valis, and Prevotella intermedia. The minimum inhibitory concentrations (MIC) of gatifloxacin and its bactericidal effects were investigated. Gatifloxacin inhibited the growth of all three kinds of periodontopathic bacteria tested in broth. The MIC value of 2.5 nM was found to be the most effective in inhibiting A. actinomycetemcomitans. An adenosine triphosphate biolumi-nescence assay revealed that gatifloxacin exhibited bactericidal effects on the tested bacteria in a time-dependent manner. The safety of gatifloxacin in mammalian cells was evaluated by assessing the viability of normal human dermal fibroblast (NHDF) cells treated with gatifloxacin. Almost all NHDF cells survived after 2-d culture, while 81% of the cells survived after 4-d culture when treated with 1.0 × 103 nM gatifloxacin. These results indicate that gatifloxacin is a possible drug for local administration to prevent periodontal infection.

关键词： Local Administration Peri-Implantitis Periodontopathic Bacteria Antimicrobial Activity Gatifloxacin

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A chemo-mechanical switchable valve on microfluidic chip based on a thermally responsive block copolymer

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Chinese Chemical Letters 2022年第6期33卷 3083-3086页

作者： Sifeng Mao Xiaohong Hu Yumi Tanaka Lin Zhou Chenhan Peng nahoko Kasai Hizuru Nakajima Shungo Kato Katsumi Uchiyama Department of Applied Chemistry Graduate School of Urban Environmental SciencesTokyo Metropolitan UniversityHachioji-shiTokyo 192-0397Japan University Education Center Tokyo Metropolitan UniversityHachioji-shiTokyo 192-0397Japan

Microfluidic devices have become a powerful tool for chemical and biologic *** control different functional parts on the microchip,valve plays a key role in the *** conventional methods,physio-mechanical valves are usually used on microfluidic ***,we reported a chemo-mechanical switchable valve on microfluidic chip by using a thermally responsive block *** wettability changes of capillary with copolymer modification on inner surface were investigated to verify the function as a *** with modification of poly-(N-isopropylacrylamide-co-hexafluoroisopropyl acrylate)(P(NIPAAm-co-HFIPA))with a 20%HFIPA was demonstrated capable of control aqueous solution stop or go *** short capillaries with copolymer modification were integrated in microchannels as *** the temperature changing around lower critical solution temperature(LCST),the integrated chemo-mechanical switchable valve exhibited excellent“OPEN–CLOSE”behavior for microflow *** optimization of the block copolymer sequences and molar ratio,a switching time as low as 20 s was *** developed micro valve was demonstrated effective for flow control on microchip.

关键词： Microchip Valve Flow control NIPPAm Chemo-mechanical switch Thermo-responsive polymer

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A Survey of Community Pharmacists about Work-Related Issues, How they Solve them, and who they Consult with to Solve them: Comparison of Results from the Hokkaido Area vs. a Nationwide Survey

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Pharmacology & Pharmacy 2018年第10期9卷 447-456页

作者： Sumiyo Umeda Hitomi Yanaguimoto Seiichi Furuta nahoko Kurosawa Faculty of Pharmaceutical Sciences Hokkaido University of Science Sapporo Japan

We surveyed the drug information retrieval practices among community pharmacists in more rural Hokkaido and in entire Japan in order to explore local characteristics of work-related issues, how community pharmacists access information to address such issues, and with whom they consult to solve them. Based on the findings, we propose a strategy for improvements in similar support systems. The percentage of respondents who had experience with home care services was significantly lower in the Hokkaido group (56.0% of 207) than in the nationwide group (70.0% of 250), as was the percentage of respondents who consulted the pharmacist association and branch board (13.0% and 20.4%, respectively). The Hokkaido group also made significantly less use of websites such as *** and ***. The results of this survey thus indicate that the drug information retrieval in the Hokkaido group had a low implementation rate of home care services. In addition, there were low levels of utilization of the local pharmacist association, and low utilization of the websites *** and ***. To enhance the Hokkaido community-based integrated care system (and ones like it), we propose that it is necessary to: 1) support activities of local pharmacist associations, and 2) promote proactive implementation of drug information retrieval through education.

关键词： Drug Informatics Pharmacy Drug Information Retrieval Hokkaido Japan

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Inhibition of Lon protease by bacterial lipopolisaccharide (LPS) though inhibition of ATPase

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Advances in Bioscience and Biotechnology 2013年第4期4卷 590-598页

作者： nahoko Sugiyama Noriko Minami Yoshiyuki Ishii Fumio Amano Division of Molecular Genetics National Institute of Infectious Diseases Tokyo Japan Laboratory of Biodefense & Regulation Osaka University of Pharmaceutical Sciences Osaka Japan

Lon protease, an ATP-dependent protease in Escherichia coli, degrades abnormal proteins and regulates several important cellular functions. Here we show novel inhibitory effects of lipopolysaccharide (LPS) on Lon protease activities. LPS inhibited the peptidase, protease, and ATPase activities of Lon;and a dose-response study showed that LPS at low doses more effectively inhibited the ATPase activity than the peptidase one, suggesting different susceptibility to LPS of these activities associated with Lon. Structure-activity relationship studies revealed that ReLPS, detoxified LPS, and mono-phosphoryl as well as diphosphoryl lipid A, also showed similar inhibition, suggesting that neither O-antigen polysaccharide nor O-acyl chain, but rather phosphate groups in the lipid A domain, seem to have been responsible for the inhibitory effects. Besides, LPS was co-precipitated with Lon by an anti-Lon antibody, showing the direct binding of LPS to Lon. These results suggest that LPS bound to Lon and inhibited the protease activity of Lon by inhibiting its ATPase activity. These results also seem to be another example of a negatively charged phosphate group in membrane components of Escherichia coli being involved in the regulation of protease activity of Lon through binding to Lon and inhibiting its ATPase activity, as in the case of cardiolipin.

关键词： ATPase Lipid A Lipopolysaccharide (LPS) Lon Protease

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Effect of Japanese knotweed(Fallopia japonica)Mulching on Continuous Potato Cropping:Modern Evaluation of Traditional Japanese Knotweed-mulch Farming in Nishi-Awa Steep Slope Land Agriculture System,Japan

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Journal of Resources and Ecology 2021年第2期12卷 254-259页

作者： INAGAKI Hidehiro UNNO nahoko SAKAKIBARA Takumi KUBOTA Sakiko HASEGAWA Kana Shizuoka University KariyadoFujiedaShizuoka 426-0001Japan

Poaceae species such as silver grass or reed are commonly used in traditional mulch farming in Japan,where the Nishi-Awa area is a Globally Important Agricultural Heritage Systems(GIAHS)*** here have traditionally used silver grass for mulch farming;furthermore,local farmers have learned from long-standing experience that Japanese knotweed(Fallopia japonica,Polygonaceae)is better for cultivation of solanaceous crops in this ***,it is unclear why Japanese knotweed mulching is beneficial for cultivation of solanaceous ***,in this study,we hypothesized that Japanese knotweed mulching may be effective in avoiding hazards associated with continuous potato cropping,as native potato used to be cultivated twice a year in the ***,we investigated the effects of Japanese knotweed mulching on continuous potato cropping and after tomato cropping,which is another solanaceous crop *** experiments were conducted in ***,we compared Japanese knotweed mulching,silver grass mulching and no grass mulching(control)in a soil under continuous potato cultivation and in an uncultivated *** a result,the extent of the potato yield decrease was reduced by Japanese knotweed ***,we compared Japanese knotweed-mulching and no grass mulching in a soil after a tomato crop and in an uncultivated *** extent of decrease in potato growth and yield was also reduced by Japanese knotweed *** findings indicate that mulching with Japanese knotweed helped to avoid the risks associated with continuous potato cropping.

关键词： continuous cropping Globally Important Agricultural Heritage Systems(GIAHS) Japanese knotweed mulch farming potato traditional farming

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Deep learning-based magnetic resonance imaging reconstruction for improving the image quality of reduced-field-of-view diffusionweighted imaging of the pancreas

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World Journal of Radiology 2023年第12期15卷 338-349页

作者： Yukihisa Takayama Keisuke Sato Shinji Tanaka Ryo Murayama nahoko Goto Kengo Yoshimitsu Department of Radiology Faculty of MedicineFukuoka UniversityFukuoka 8140180Japan

BACKGROUND It has been reported that deep learning-based reconstruction(DLR)can reduce image noise and artifacts,thereby improving the signal-to-noise ratio and image ***,no previous studies have evaluated the efficacy of DLR in improving image quality in reduced-field-of-view(reduced-FOV)diffusionweighted imaging(DWI)[field-of-view optimized and constrained undistorted single-shot(FOCUS)]of the *** hypothesized that a combination of these techniques would improve DWI image quality without prolonging the scan time but would influence the apparent diffusion coefficient *** To evaluate the efficacy of DLR for image quality improvement of FOCUS of the *** This was a retrospective study evaluated 37 patients with pancreatic cystic lesions who underwent magnetic resonance imaging between August 2021 and October *** evaluated three types of FOCUS examinations:FOCUS with DLR(FOCUS-DLR+),FOCUS without DLR(FOCUS-DLR−),and conventional FOCUS(FOCUS-conv).The three types of FOCUS and their apparent diffusion coefficient(ADC)maps were compared qualitatively and *** FOCUS-DLR+(3.62,average score of two radiologists)showed significantly better qualitative scores for image noise than FOCUS-DLR−(2.62)and FOCUS-conv(2.88)(P<0.05).Furthermore,FOCUS-DLR+showed the highest contrast ratio and 600 s/mm^(2)(0.72±0.08 and 0.68±0.08)and FOCUS-DLR−showed the highest CR between cystic lesions and the pancreatic parenchyma for the b-values of 0 and 600 s/mm2(0.62±0.21 and 0.62±0.21)(P<0.05),***-DLR+provided significantly higher ADCs of the pancreas and lesion(1.44±0.24 and 3.00±0.66)compared to FOCUS-DLR−(1.39±0.22 and 2.86±0.61)and significantly lower ADCs compared to FOCUS-conv(1.84±0.45 and 3.32±0.70)(P<0.05),*** This study evaluated the efficacy of DLR for image quality improvement in reduced-FOV DWI of the *** can significantly denoise images without prolonging the scan time or

关键词： Deep learning-based reconstruction Magnetic resonance imaging Reduced field-of-view Diffusion-weighted imaging Pancreas

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Suppressive Effects of Traditional Mulching Using Japanese Knotweed(Fallopia japonica)on Solanaceae Crop Diseases

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Journal of Resources and Ecology 2021年第6期12卷 869-875页

作者： INAGAKI Hidehiro KUBOTA Sakiko HASEGAWA Kana UNNO nahoko USUI Yukiko TAKIKAWA Yuichi Shizuoka University Shizuoka 422-8529Japan

Poaceae plant species,such as silver grass,are commonly used in mulching activities *** contrast,local farmers have traditionally used Japanese knotweed(Fallopia japonica)mulch in the cultivation of solanaceous crops in the Nishi-Awa area of Japan,which is a Globally Important Agricultural Heritage Systems *** have previously evaluated the positive effects of Japanese knotweed mulching on solanaceous crops,such as eggplants,tomato,and *** the present study,we observed that the naturally occurring diseases in the solanaceous crops tended to decrease when the knotweed mulching system was adopted,in comparison to when Poaceae mulch was *** eggplants,leaf mold and powdery mildew decreased under Japanese knotweed *** further evaluated the effects of Japanese knotweed mulching by inoculating test plants with Pseudomonas *** observed suppression of bacterial disease and tomato mosaic virus under Japanese knotweed mulching and following spraying with Japanese knotweed *** addition,disease-resistance genes were expressed at high levels in Arabidopsis thaliana,a model plant,following treatment with Japanese knotweed *** results suggest that Japanese knotweed has potential applications in future sustainable agriculture activities.

关键词： Traditional Ecological Knowledges(TEK) Globally Important Agricultural Heritage Systems(GIAHS) Japanese knotweed Solanaceae mulching systemic acquired resistance(SAR)

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Clarifying Problems with Emergency Healthcare Systems in Japanese Long-Term Care Facilities for Older People

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Health 2017年第8期9卷 1159-1175页

作者： Nobuhiro Sato Kohei Akazawa Yuji Mitadera Tsubasa Suzuki nahoko Ibe Yasuo Hirose Department of Medical Informatics and Statistics Niigata University Graduate School of Medicine Niigata Japan Department of Emergency and Critical Care Medicine Niigata City General Hospital Niigata Japan Development Center of Medical Professionals for Next-Generation Niigata University Hospital Niigata Japan Patient and Family Support Center Niigata City General Hospital Niigata Japan

Background: Long-term care facilities for older people play an important role as alternatives to family care in an aging society. This study aimed to assess staffing problems linked to emergency healthcare for residents of these facilities, and to investigate the relationship between these problems and the characteristics of the facilities. Methods: The study surveyed managers of long-term care facilities for older people in a Japanese urban area between September and December 2014. The type of care facility, the number of staff and any problems providing or accessing emergency healthcare for the residents were evaluated. Multiple logistic regression analysis was used to explore the factors common to facilities reporting staffing problems linked to emergency healthcare of residents. Results: In total, 321 long-term care facilities were eligible for this study and 226 (70%) returned the questionnaire. We compared the characteristics of facilities reporting and not reporting staffing problems in emergency healthcare of residents. The type of care facility was significantly associated with staffing problems in emergency healthcare of residents both during the day (p < 0.01) and at night (p = 0.04). The facilities most likely to report problems were group homes for older people with dementia, because of staffing shortages. Conclusions: Problems in emergency healthcare systems in Japanese long-term care facilities for older people varied by type of care facility. Our data underscore the need for telemedicine and consideration of mergers between smaller facilities such as group homes for older people with dementia.

关键词： Older People Emergency Healthcare System Japan Long-Term Care Staffing Problems

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An Efficient Synthesis of 2,3-Diaminoacid Derivatives Using Phosphine Catalyst

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International Journal of Organic Chemistry 2014年第3期4卷 189-194页

作者： Yohei Oe Hiroaki Kishimoto nahoko Sugioka Daisuke Harada Yukio Sato Tetsuo Ohta Isao Furukawa Department of Biomedical Information Faculty of Life and Medical Sciences Doshisha University Kyoto Japan Department of Molecular Science and Technology Faculty of Engineering Doshisha University Kyoto Japan

Ethyl 2,3-diphthalimidoylpropanoate was effectively synthesized from ethyl propynoate with two equivalents of phthalimide catalyzed by triphenylphosphine in good yield. The choice of reaction media was important for selective synthesis of the desired 2,3-diaminocarboxylic acid derivatives. The reaction is considered to occur through a zwitterionic intermediate derived from the reaction of the α,β-unsaturated ester with triphenylphosphine.

关键词： Addition Alkynes Amino Acids Catalysis Multi-Component Reaction

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