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移植的齿状回颗粒细胞在培养的大鼠海马组织上移行特征

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神经解剖学杂志 2007年第1期23卷 15-19页

作者：韩春锡文善姬河西奈保子小林未明廖建湘鸟光庆一深圳市儿童医院神经内科长春市妇幼保健院长春130000 NTT物性科学研究所

为探讨齿状回颗粒细胞在培养的海马组织表面上的移行过程以及苔状纤维的投射特征,本实验从生后3d的绿色荧光蛋白(GFP)基因导入的SD大鼠海马组织切片中分离出齿状回颗粒细胞层,移植到培养的SD大鼠海马组织切片表面上,共同培养3～4d后,激... 详细信息

为探讨齿状回颗粒细胞在培养的海马组织表面上的移行过程以及苔状纤维的投射特征,本实验从生后3d的绿色荧光蛋白(GFP)基因导入的SD大鼠海马组织切片中分离出齿状回颗粒细胞层,移植到培养的SD大鼠海马组织切片表面上,共同培养3～4d后,激光共聚焦显微镜观察。结果显示,97.6%的细胞移行到齿状回和CA3区,仅有2.4%细胞移行到CA2和CA1区。移行到齿状回颗粒细胞层的颗粒细胞投射苔状纤维到齿状回门区和CA3区的透明层,却不到CA2和CA1区。上述结果提示,在海马组织培养中移植的齿状回颗粒细胞的移行以及苔状纤维的投射方向明显受宿主的影响。

关键词：海马组织培养齿状回颗粒细胞细胞移行苔状纤维 GFP

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日本心血管疾病患者的安宁疗护及启示

中华护理杂志 2024年第5期59卷 533-539页

作者：彭梦云原田奈穂子齋藤信也岡山大学保健学研究科日本岡山市7008558 岡山大学医学部保健学科日本岡山市7008558

WHO呼吁全球医疗保健工作者重视非癌症患者的安宁疗护实践,目前国内安宁疗护主要以癌症患者为中心,在心血管疾病等慢性病人群中的实践和发展相对薄弱。近年来,日本开展的心血管疾病安宁疗护经实践验证可有效缓解患者的终末期症状,提高... 详细信息

WHO呼吁全球医疗保健工作者重视非癌症患者的安宁疗护实践,目前国内安宁疗护主要以癌症患者为中心,在心血管疾病等慢性病人群中的实践和发展相对薄弱。近年来,日本开展的心血管疾病安宁疗护经实践验证可有效缓解患者的终末期症状,提高患者及家庭成员的生活质量。该文介绍了日本心血管疾病患者安宁疗护的发展历程、规模及需求、团队成员及协作、介入时间、评估与筛查、服务内容与流程、质量评价及困境等,提出对国内心血管疾病患者实施安宁疗护的启示,为开展心血管疾病患者的安宁疗护提供借鉴。

关键词：心血管疾病安宁疗护姑息护理

移植齿状回颗粒细胞重建海马神经网络的方法研究

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中国临床神经科学 2007年第6期15卷 614-619页

作者：韩春锡文善姬河西奈保子廖建湘鸟光庆一深圳市儿童医院神经内科儿科研究所日本厚木NTT物性科学基础研究所

目的:探讨新生大鼠齿状回颗粒细胞在培养的海马组织切片上迁移特征。方法:从生后3d的绿色荧光蛋白(GFP)基因导入的SD大鼠海马组织中分离颗粒细胞层组织,分别移植到生后7d的宿主(野生型)SD大鼠海马组织切片的不同区域,施行共同培养6d,观... 详细信息

目的:探讨新生大鼠齿状回颗粒细胞在培养的海马组织切片上迁移特征。方法:从生后3d的绿色荧光蛋白(GFP)基因导入的SD大鼠海马组织中分离颗粒细胞层组织,分别移植到生后7d的宿主(野生型)SD大鼠海马组织切片的不同区域,施行共同培养6d,观察表达GFP的齿状回颗粒细胞的迁移方向和纤维投射。结果:当移植物移植到齿状回门区附近时,表达GFP的齿状回颗粒细胞迁移到宿主海马组织的齿状回门区和颗粒细胞层,并发出树突和轴突分别投射到齿状回的内分子层和CA3区。结论:移植的齿状回颗粒细胞在海马组织切片上迁移并发出神经纤维投射到CA3区,提示移植的齿状回颗粒细胞可能参与海马的局部神经网络。

关键词：海马组织培养移植颗粒细胞迁移绿色荧光蛋白

荷包牡丹碱对海马CA2区的神经毒性与P/Q型钙通道有关

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中国临床神经科学 2008年第5期16卷 458-462页

作者：韩春锡文善姬河西奈保子岳丽杰廖建湘鸟光庆一深圳市儿童医院神经内科儿科研究所 NTT物性科学基础研究所

目的:探讨荷包牡丹碱(BiC)对海马CA2区的神经毒性作用以及电压依赖性钙通道(VDCC)对神经毒性作用的影响。方法:BiC 6μmol·L-1刺激培养的大鼠海马组织72 h,同时用N,L以及P/Q型钙通道拮抗剂分别阻断相应的钙通道,测定神经细胞摄取的碘... 详细信息

目的:探讨荷包牡丹碱(BiC)对海马CA2区的神经毒性作用以及电压依赖性钙通道(VDCC)对神经毒性作用的影响。方法:BiC 6μmol·L-1刺激培养的大鼠海马组织72 h,同时用N,L以及P/Q型钙通道拮抗剂分别阻断相应的钙通道,测定神经细胞摄取的碘化丙啶(PI)。结果:BiC刺激6 h后,神经细胞的PI摄取首先出现在CA2区。随着BiC刺激时间的延长,摄取PI的神经细胞的分布范围由CA2区扩散到其他区域。P/Q型VDCC拮抗剂抑制神经细胞的PI摄取,然而N和L型VDCC的拮抗剂无明显抑制效果。结论:在大鼠海马的组织培养中CA2区的神经细胞对BiC的刺激最易受损。CA2区神经细胞的损伤过程中P/Q型VDCC起重要作用。

关键词：荷包牡丹碱 CA2区海马组织培养细胞死亡 P/Q型钙通道

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中国美术学院博物馆

城市环境设计 2024年第1期147卷 76-81页

作者：藤原徹平寺川奈穂子小岛伸也加纳永一

中国美术学院博物馆位于中国美院杭州象山校区，坐落在一片曾经的茶场上。为了与大地融为一体，创造出能够感受大地的博物馆，沿着山坡的倾斜地面进行了连续的设计。通过以平行四边形为单位的几何分割系统，进行了复杂地形的规划，并通...

中国美术学院博物馆位于中国美院杭州象山校区，坐落在一片曾经的茶场上。为了与大地融为一体，创造出能够感受大地的博物馆，沿着山坡的倾斜地面进行了连续的设计。通过以平行四边形为单位的几何分割系统，进行了复杂地形的规划，并通过为每个单位建造小屋顶，形成了连续的瓦屋顶的“村庄”景观。外墙上覆盖着一道“屏风”，由不锈钢丝外挂瓷砖构成，有助于控制进入房间的阳光。屏风和屋顶的旧瓷砖都来自本地烧的瓦。瓦片尺寸的变化使建筑更好地与大地融合。

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A Survey of Community Pharmacists about Work-Related Issues, How they Solve them, and who they Consult with to Solve them: Comparison of Results from the Hokkaido Area vs. a Nationwide Survey

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Pharmacology & Pharmacy 2018年第10期9卷 447-456页

作者： Sumiyo Umeda Hitomi Yanaguimoto Seiichi Furuta nahoko Kurosawa Faculty of Pharmaceutical Sciences Hokkaido University of Science Sapporo Japan

We surveyed the drug information retrieval practices among community pharmacists in more rural Hokkaido and in entire Japan in order to explore local characteristics of work-related issues, how community pharmacists access information to address such issues, and with whom they consult to solve them. Based on the findings, we propose a strategy for improvements in similar support systems. The percentage of respondents who had experience with home care services was significantly lower in the Hokkaido group (56.0% of 207) than in the nationwide group (70.0% of 250), as was the percentage of respondents who consulted the pharmacist association and branch board (13.0% and 20.4%, respectively). The Hokkaido group also made significantly less use of websites such as *** and ***. The results of this survey thus indicate that the drug information retrieval in the Hokkaido group had a low implementation rate of home care services. In addition, there were low levels of utilization of the local pharmacist association, and low utilization of the websites *** and ***. To enhance the Hokkaido community-based integrated care system (and ones like it), we propose that it is necessary to: 1) support activities of local pharmacist associations, and 2) promote proactive implementation of drug information retrieval through education.

关键词： Drug Informatics Pharmacy Drug Information Retrieval Hokkaido Japan

Inhibition of Lon protease by bacterial lipopolisaccharide (LPS) though inhibition of ATPase

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Advances in Bioscience and Biotechnology 2013年第4期4卷 590-598页

作者： nahoko Sugiyama Noriko Minami Yoshiyuki Ishii Fumio Amano Division of Molecular Genetics National Institute of Infectious Diseases Tokyo Japan Laboratory of Biodefense & Regulation Osaka University of Pharmaceutical Sciences Osaka Japan

Lon protease, an ATP-dependent protease in Escherichia coli, degrades abnormal proteins and regulates several important cellular functions. Here we show novel inhibitory effects of lipopolysaccharide (LPS) on Lon protease activities. LPS inhibited the peptidase, protease, and ATPase activities of Lon;and a dose-response study showed that LPS at low doses more effectively inhibited the ATPase activity than the peptidase one, suggesting different susceptibility to LPS of these activities associated with Lon. Structure-activity relationship studies revealed that ReLPS, detoxified LPS, and mono-phosphoryl as well as diphosphoryl lipid A, also showed similar inhibition, suggesting that neither O-antigen polysaccharide nor O-acyl chain, but rather phosphate groups in the lipid A domain, seem to have been responsible for the inhibitory effects. Besides, LPS was co-precipitated with Lon by an anti-Lon antibody, showing the direct binding of LPS to Lon. These results suggest that LPS bound to Lon and inhibited the protease activity of Lon by inhibiting its ATPase activity. These results also seem to be another example of a negatively charged phosphate group in membrane components of Escherichia coli being involved in the regulation of protease activity of Lon through binding to Lon and inhibiting its ATPase activity, as in the case of cardiolipin.

关键词： ATPase Lipid A Lipopolysaccharide (LPS) Lon Protease

Effect of Japanese knotweed(Fallopia japonica)Mulching on Continuous Potato Cropping:Modern Evaluation of Traditional Japanese Knotweed-mulch Farming in Nishi-Awa Steep Slope Land Agriculture System,Japan

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Journal of Resources and Ecology 2021年第2期12卷 254-259页

作者： INAGAKI Hidehiro UNNO nahoko SAKAKIBARA Takumi KUBOTA Sakiko HASEGAWA Kana Shizuoka University KariyadoFujiedaShizuoka 426-0001Japan

Poaceae species such as silver grass or reed are commonly used in traditional mulch farming in Japan,where the Nishi-Awa area is a Globally Important Agricultural Heritage Systems(GIAHS)*** here have traditionally used silver grass for mulch farming;furthermore,local farmers have learned from long-standing experience that Japanese knotweed(Fallopia japonica,Polygonaceae)is better for cultivation of solanaceous crops in this ***,it is unclear why Japanese knotweed mulching is beneficial for cultivation of solanaceous ***,in this study,we hypothesized that Japanese knotweed mulching may be effective in avoiding hazards associated with continuous potato cropping,as native potato used to be cultivated twice a year in the ***,we investigated the effects of Japanese knotweed mulching on continuous potato cropping and after tomato cropping,which is another solanaceous crop *** experiments were conducted in ***,we compared Japanese knotweed mulching,silver grass mulching and no grass mulching(control)in a soil under continuous potato cultivation and in an uncultivated *** a result,the extent of the potato yield decrease was reduced by Japanese knotweed ***,we compared Japanese knotweed-mulching and no grass mulching in a soil after a tomato crop and in an uncultivated *** extent of decrease in potato growth and yield was also reduced by Japanese knotweed *** findings indicate that mulching with Japanese knotweed helped to avoid the risks associated with continuous potato cropping.

关键词： continuous cropping Globally Important Agricultural Heritage Systems(GIAHS) Japanese knotweed mulch farming potato traditional farming

Effect of zeta potentials on bovine serum albumin adsorption on crown composite resin surfaces <i>in vitro</i>

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Journal of Biomedical Science and Engineering 2013年第3期6卷 273-276页

作者： nahoko Miyake Tadashi Miura Toru Sato Masao Yoshinari Department of Crown & Bridge Prosthodontics Tokyo Dental College Chiba Japan Division of Oral Implants Research Oral Health Science Center Tokyo Dental College Chiba Japan Division of Prosthodontics Department of Clinical Oral Health Science Tokyo Dental College Tokyo Japan

We previously studied the mechanism underlying the adsorption of oral bacteria on the surfaces of dental prosthetic materials such as ceramics and resins in vitro. The aim of the present study was to examine bovine serum albumin (BSA) adsorption on crown composite resin surfaces by means of zeta potential. We measured the zeta potentials of resins alone, BSA alone, and resins after BSA adsorption. Eight resins were pulverized into powders (300 - 1000 nm). All experiments were conducted in 10 mM sodium chloride solution (pH 6.5). BSA was dissolved in 10 mM NaCl with a concentration of 2.0 × 10-5 mol/l. An adsorption assay was performed for one hour at 37°C under continuous rotation (6 rpm). The zeta potentials of both resins and BSA were negative, with BSA itself less negative than the resins themselves as an absolute value (p < 0.0001). The zeta potentials of seven resin surfaces after BSA adsorption were significantly less negative than were those of the resins without BSA adsorption (p < 0.0001). Eight resins were divided into two classes based on the size of the surface potential difference between each resin and the BSA. The difference in surface potential between the resins and the BSA were small, leading to the theory that particles with identical charges repulse each other, and the amounts of adsorbed BSA on these resins might be less. On the other, when the differences between the other resins and BSA are large, so that the repulsive force between two nonidentical particles becomes zero and an attractive force might be generated, then more BSA might be adsorbed on those resins. Therefore, the zeta potentials were affected by BSA adsorption and became less negative. These results suggested that electrostatic interactions play an important role in the adsorption of BSA on resin surfaces.

关键词： Zeta Potential Crown Composite Resins Bovine Serum Albumin Adsorption Electrostatic Interactions