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Angiogenesis Factors Associated with New Breast Cancer Cell Line AMJ13 Cultured <i>in Vitro</i>

Advances in Breast Cancer Research 2015年第4期4卷 100-108页

作者： Ahmed Majeed Al-Shammari Worod Jawad Kadhim Allak Mahfoodha Umran nahi y. yaseen Ay.an Hussien Experimental Therapy.Department Iraqi Center for Cancer and Medical Genetic Research Al-Mustansiriyah University Baghdad Iraq Biotechnology.Department Collage of Science Baghdad University Baghdad Iraq

Background: AMJ13 is a new breast cancer cell line that has been established from a 70-y.ar-old Iraqi woman with a histological diagnosis of infiltrating ductal carcinoma. It is the first for an Iraqi population. In breast cancer, angiogenesis provides the tumor tissue, which is rapidly.proliferated with oxy.en and nutrients, removes wastes and increases the opportunity.of cancer cells to invade other organs. Methods: The AMJ13 breast cancer cell line was represented at three different passages and incubated for interval times. Microarray.panel of 43 different angiogenesis markers was used to scan the supernatant for the factors. ELISA was used to quantify.some of the important angiogenesis factors released in the culture medium and to confirm absence of those who was not detected by.the antibody.array. RT-PCR was used to confirm the gene expression (mRNA) of studied factors. Results: Microarray.analy.is showed that TIMP1 and two secreted at highest levels compared to the rest of the factors with low presence of endostatin. Other non-detectable factors by.microarray.examined by.ELISA assay.that showed highest expression level of VEGF-A were obtained at earliest passage, while the highest levels of FGF-b were obtained at late passage. The VEGF-D secretion was shown low concentrations at all studied passages. There is no detectable level of EGF protein in different passages and times interval tested. There are no significant differences in secretion of sICAM between different passages and incubation periods. Conclusion is that AMJ13 cell line depends on VEGF-A as main angiogenesis factor to induce micro-vessels supported by.low levels of VEGF-D for ly.phatic vessels formation. AMJ13 cell line depends on FGF as growth factors as in late passages it was shifted to depend mainly.on FGF completely. All of this process may.be regulated by.TGF-β. TIMP-1 has proangiogentic effect and has feedback talk with TIMP-2. Understanding the angiogenesis process for breast cancer can

关键词： Angiogenesis Factors Breast Cancer Cell AMJ13 Tumor Tissue

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BM-MSCs Differentiated Insulin-Producing Cells Produce More Insulin in Presence of EGF than of FGF

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Stem Cell Discovery 2015年第4期5卷 33-39页

作者： Cheia Majeed Ahmed M. Al-Shammari Eman H. y.usif nahi y. yaseen Ay.an Ali Husien Rajha A. Al-Naimi Adnan Ajeel Department of Pathology College of Veterinary Medicine Baghdad University Baghdad Iraq Experimental Therapy.Department Iraqi Center for Cancer and Medical Genetic Research Al-Mustansiriya University Baghdad Iraq

Adult stem cells have the ability.to differentiate into islet like cells for the hope of treating diabetes mellitus (DM). The most important part is the differentiation process from na?ve stem cells to fully.differentiated fully.functional islet cells. For this purpose, we wanted to optimize the current proposed differentiation media by.replacing the FGF with EGF and measure insulin production. Bone marrow-derived MSCs were from mice long bones and expanded in cell culture before induction of differentiation. Stem cells surface markers were analy.ed by.immunocy.ochemistry. Cultured stem cells were negative for CD34 while they.expressed high levels of CD90. Differentiated cells morphology.was studied by.using H & E stain. Differentiated cells were detected by.study.ng protein expression of insulin as specific marker for IPC differentiation. Cells function was studied by.measuring the insulin production in tissue culture supernatant in vitro and also insulin release in response to glucose challenge. Ditizone staining were both positive. Insulin was secreted by.these cells in response to different concentrations of glucose stimulation in a regulated manner. Cells induced with formula contain EGF produced more insulin in the same formula but contain FGF instead, this prove that EGF is the best to use during differentiation process.

关键词： Diabetes Mellitus Pancreatic β Cells Growth Factors

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