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Monitoring adenoviral based gene delivery in rat glioma by molecular imaging

World Journal of Clinical Oncology 2013年第4期4卷 91-101页

作者： nadimpalli ravi s varma Kenneth N Barton Branislava Janic Adarsh shankar AsM Iskander Meser M Ali Ali s Arbab Cellular and Molecular Imaging Laboratory Department of RadiologyHenry Ford HospitalDetroitMI 48202United States Department of Radiation Oncology Henry Ford HospitalDetroitMI 48202United States

AIM:To determine whether endothelial progenitor cells(EPCs)can be used as delivery vehicle for adenoviral vectors and imaging probes for gene therapy in ***:To use cord blood derived EPCs as delivery vehicle for adenoviral vectors and imaging probes for glioma gene therapy,a rat model of human glioma was made by implanting U251 cells *** were transfected with an adenovirus(AD5/carrying hNIs gene)and labeled with iron oxide and inoculated them directly into the tumor 14 d following implantation of U251 *** resonance imaging(MRI)was used to in vivo track the migration of EPCs in the *** expression of gene products was determined by in vivo Tc-99m single photon emission computed tomography(sPECT).The findings were validated with immunohistochemistry(IHC).REsULTs:EPCs were successfully transfected with the adenoviral vectors carrying hNIs which was proved by significantly(Psfected *** of EPCs following transfection and iron labeling was not *** vivo imaging showed the presence of iron positive cells and the expression of transgene(hNIs)product on MRI and sPECT,respectively,all over the tumors following administration of transfected and iron labeled EPCs in the *** confirmed the distribution of EPC around the tumor away from the injection site and also showed transgene expression in the *** results indicated the EPCs’ability to deliver adenoviral vectors into the glioma upon intratumor ***:EPCs can be used as vehicle to deliver adenoviral vector to glioma and also act as imaging probe at the same time.

关键词： Cord blood endothelial progenitor cells Adenovirus Human sodium iodide symporter single photon emission computed tomography Magnetic resonance imaging

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subcurative radiation significantly increases cell proliferation, invasion, and migration of primary glioblastoma multiforme in vivo

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Chinese Journal of Cancer 2014年第3期33卷 148-158页

作者： Adarsh shankar sanath Kumar Asm Iskander nadimpalli Rs varma Branislava Janic Ana deCarvalho Tom Mikkelsen Joseph A Frank Meser M Ali Robert A Knight stephen Brown Ali s Arbab Department of Radiology Henry Ford HospitalDepartment of Radiation OncologyHenry Ford HospitalDepartment of NeurosurgeryHenry Ford HospitalLaboratory of Diagnostic Radiology ResearchNational Institutes of HealthDepartment of Radiation OncologyWayne State University School of MedicineDepartment of RadiologyWayne State University School of MedicineDepartment of NeurologyHenry Ford Hospital

Tumor cell proliferation, infiltration, migration, and neovascularization are known causes of treatment resistance in glioblastoma multiforme(GBM). The purpose of this study was to determine the effect of radiation on the growth characteristics of primary human GBM developed in a nude rat. Primary GBM cells grown from explanted GBM tissues were implanted orthotopically in nude rats. Tumor growth was confirmed by magnetic resonance imaging on day 77(baseline) after implantation. The rats underwent irradiation to a dose of 50 Gy delivered subcuratively on day 84 postimplantation(n = 8), or underwent no radiation(n = 8). Brain tissues were obtained on day 112(nonirradiated) or day 133(irradiated). Immunohistochemistry was performed to determine tumor cell proliferation(Ki-67) and to assess the expression of infiltration marker(matrix metalloproteinase-2, MMP-2) and cell migration marker(CD44). Tumor neovascularization was assessed by microvessel density using von-Willebrand factor(vWF) staining. Magnetic resonance imaging showed well-developed, infiltrative tumors in 11 weeks postimplantation. The proportion of Ki-67-positive cells in tumors undergoing radiation was(71 ± 15)% compared with(25 ± 12)% in the nonirradiated group(P = 0.02). The number of MMP-2-positive areas and proportion of CD44-positive cells were also high in tumors receiving radiation, indicating great invasion and infiltration. Microvessel density analysis did not show a significant difference between nonirradiated and irradiated tumors. Taken together, we found that subcurative radiation significantly increased proliferation, invasion, and migration of primary GBM. Our study provides insights into possible mechanisms of treatment resistance following radiation therapy for GBM.

关键词： Glioblastoma multiforme radiation treatment resistance invasion

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Effects of Thermal Radiation and Radiation Absorption on Flow Past an Impulsively started Infinite Vertical Plate with Newtonian Heating and Chemical Reaction

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Open Journal of Fluid Dynamics 2015年第4期5卷 364-379页

作者： swetha ravi Jagdish Prakash Viswanatha Reddy Gottam Vijaya Kumar varma sibyala Department of Mathematics Gudlavalleru Engineering College Gudlavalleru India Department of Mathematics University of Botswana Gaborone Botswana Department of Mathematics S. V. University Tirupati India

A perfect solution to the present natural convective flow problem of a vertical transfinite plate owing to the impulsive motion in the ubiety of first ordered chemical reaction, radiation absorption, radiation, Newtonian heating and species concentration in its plane is evolved by applying the method of Laplace transforms in closed form at the plate. Exact results for velocity, temperature, concentration fields are prevailed and expressions for heat and mass transfer rates are also found. The effects are analyzed for the respective invariables for both ammonia and water vapor.

关键词： Newtonian Heating Natural Convection Chemical Reaction Incompressible Fluid Radiation Absorption and Radiation

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