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Biochemical Characterization of Lipase Produced by <i>Bacillus</i>spp. isolated from Soil and Oil Effluent

Advances in Enzyme Research 2020年第4期8卷 39-48页

作者： Afaf O. B. Shart Elhadi A. i. Elkhalil Department of Botany and Agric. Biotechnology Faculty of Agriculture University of Khartoum Shambat Sudan

The aim of the present work was to isolate Bacillus spp. With high lipase activity;to characterize the isolates using both biochemical and molecular methods;to produce lipase using Bacillus isolates and to study the biochemical and biophysical characteristics of the produced lipase. Sixty five Bacillus isolates were isolated from soil 20 isolates from guar field soil (G), 15 isolates from Abusabein field soil (B), 15 isolates from sun flower field soil (S) and 15 isolates from oil effluent (O). Lipase producing isolates were screened;a Chromogenic plate’s method was used. Enzyme activity was quantitatively assayed. Lipase production under submerged fermentation (SMF) conditions using a production medium that contained metal salts, Tween-20 and olive oil as substrate at different period 24, 48, 72 and 96 h, the optimum pH, temperature for lipase activity was determinated and kinetics as well. The isolates showed the highest lipase activity which was identified as Bacillus sp. The optimum pH, temperature, thermostability and kinetic of the produced enzymes were found in three isolates G14, O1 and B10 with the highest enzyme activity and best stability. The isolates G14, O1 and B10 revealed the highest lipase activity of 63.4, 41.2 and 28.3 U/ml, respectively. The results showed optimum pH of the lipase activity from isolates G14, O1 and B10 8.0, 6.0 and 6.0 and the optimum temperature 40, 60 and 75˚C, respectively. Lipase enzymes from isolates O1 and B10 were found to be more thermostable after incubation time for 120 min at 90˚C. The Vmax and Km values of lipase for isolates G14, Oi and B1

关键词： Bacillus Lipase Biochemical Characterization Oil Effluent

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作者： i.M.·弗思

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Assessing Knowledge about the Dangers of Alcohol Consumption： A Cross-sectional Descriptive Study, among Makerere University Undergraduate Students

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Journal of Health Science 2017年第3期5卷 121-127页

作者： P. Babua Emyedu Andrew J. Nabukalu Denis Mugarura C. Ochwo T. i. Nahereza i. Ssinabulya H Wabinga Department of Medicine College of Health Sciences Makerere University Kampala P.O. Box 7062 Uganda Department of Pathology College of Health Sciences Makerere University Kampala P.O. Box 7062 Uganda

Consumption of alcohol and other alcoholic beverages is widespread in Uganda and it is the highest per capita consumer of alcohol in Africa. Many households are involved in informal alcohol production for income purposes, resulting in alcohol being easily available. Alcohol is consumed mostly by adults and young adults, waragi （a local brew） being high on the menu. There is a high prevalence of alcohol consumption at Makerere University so in our study we set out to assess the knowledge about dangers of alcohol consumption among these students. it was a cross-sectional descriptive study. The target population was undergraduate students of Makerere University. Data were collected using self-administered questionnaires and focus group discussions. We interviewed 435 University students, 236 （54.3%） males and 199 （45.7%） females. Median age was 21 years, similar across gender. There were more participants from year one to three compared to years four and five. Majority of the students were consuming alcohol 242 （55.6%）, 49 （11.3%） had stopped and 144 （33.1%） had never consumed alcohol. A higher proportion of males consumed alcohol compared to females; 60.2% and 50.2% respectively. Some reasons given for alcohol consumption were： peer pressure, coping with stressful situations, celebration and the need for adventure. The students were knowledgeable about the dangers of alcohol consumption like liver disease, violence, mental illness, cancer, diabetes mellitus, financial loss and death. Despite the vast knowledge about the dangers of alcohol consumption, some students were reluctant to stop consuming alcohol, so fighting this habit in this population is important. Parents and students need to be mindful of where to reside so as to avoid bad company. Some of the students who stopped consuming alcohol attributed it to concern from a relative, friend or health worker, so involving the entire public could go a long way in the fight against alcohol abuse as well as strict reg

关键词： Alcohol consumption Makerere University students dangers of alcohol consumption.

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Therapeutic Effect of Dimethyl Dimethoxy Biphenyl Dicarboxylate on Collagen-induced Arthritis in Rats

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Chinese Journal of integrative Medicine 2015年第11期21卷 846-854页

作者： Roba M Talaat Amira S Abo-Ei-Atta Sabah M Farou Karima i Ei-Dosoky Molecular Biology Department Genetic Engineering and Biotechnology Research InstituteMenofia UniversitySadat Branch Pathology Department Faculty of MedicineTanta University

Objective： To study the effect of oral administration of dimethyl dimethoxy biphenyl dicarboxylate （DDB） on adjusting angiogeneic/inflammatory mediators and ameliorating the pathology of bones in rats with collagen-induced arthritis （CiA）. Methods： Wistar rat model of CiA was set up using bovine collagen type H. Fifty rats were divided into five groups randomly： normal, CiA model, DDB treatment, methotrexate （MTX） treatment, and combined DDB＋MTX treatment. Ankle joints of rats were imaged with digital X-ray machine to show the destruction of joints. Fore and hind paw and knee joints were removed above the ankle joint then processed for haematoxylin and eosin staining. Plasma levels of vascular endothelial growth factor （VEGF）, platelet derived growth factor, interleukin-8 （iL-8）, iL-4, tumor necrosis factor α （TNF-α）, and cyclooxygenase-2 （COX-2） were quantified by enzyme-linked immunosorbent assay. Nitric oxide levels were detected by Griess reagent. Results： Compared with the CiA model group, a remarkable reduction in various angiogenic （VEGF and iL-8） and inflammatory mediators （TNF-α, iL-4 and COX-2） after treatment with DDB either alone or combined with MTX （P〈0.05 or P〈0.01）. Histopathological and X-ray findings were confirmatory to the observed DDB anti-arthritic effect. The DDB-treated group showed amelioration in signs of arthritis which appeared essentially similar to normal. Conclusion： Our data shed light on the therapeutic efficacy of DDB in experimental rheumatoid arthritis （RA） compared with a choice drug （MTX） and it may be offered as a second-line drug in the treatment of RA.

关键词： rheumatoid arthritis collagen-induced arthritis dimethyl dimethoxy biphenyl dicarboxylate angiogenesis inflammation

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作者： i.·阿西摩夫

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地球以外的文明世界

地球以外的文明世界

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作者： i.·阿西莫夫(i. Asimov)

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作者： i.·阿西摩夫

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我,机器人

我,机器人

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作者： i.·阿西莫夫(i. Asimov)

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医学图像的快速最优化边缘检测

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计算机学报 1997年第12期20卷 1063-1068页

作者：王合庄天戈蒋大宗 Liu W.Y.Magnin i.E.Gimenez G. 上海交通大学生物医学工程研究所法国国家应用科学院超声与信号处理研究室

在医学图像的目标识别和三维显示过程中，边缘检测是十分重要的一环，检测的准确性将直接影响到后续的治疗．虽然现在已有许多边缘提取算法，但这些方法用于医学图像分割时很少能同时满足速度快、最优化以及所检测边缘连接甚至封闭等要... 详细信息

在医学图像的目标识别和三维显示过程中，边缘检测是十分重要的一环，检测的准确性将直接影响到后续的治疗．虽然现在已有许多边缘提取算法，但这些方法用于医学图像分割时很少能同时满足速度快、最优化以及所检测边缘连接甚至封闭等要求．本文提出一种基于动态规划（ＤｇｎａｍｉｃＰｒｏｇｒａｍｍｉｎｇ，简记为ＤＰ）的边缘检测的快速算法，在算法中采用局域窗技术加快搜索速度，由分段搜索自适应地实现快速最优化巨连续的目标边缘检测．算法在模型图像及医学图像中实施，取得了满意的效果．

关键词：边缘检测图像分割医学图像图像处理

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4,4’-Methylenebis(2-chloroaniline) (MBOCA) May Be Highly Toxic and a Carcinogen Based on an Experimental Study with Mice

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Advances in Biological Chemistry 2014年第3期4卷 203-213页

作者： Hong-i Chen Tzong-Cherng Chi Shun-Yao Ko Yi-Chiang Hsu i-Hsuan Lin Ann Chen Saou-Hsing Liou Chien-Feng Li Department of Pathology Chi-Mei Medical Center Tainan Taiwan Department of Pathology Tri-Service General Hospital National Defense Medical Center Taipei Taiwan Division of Environmental Health and Occupational Medicine National Health Research Institutes Zhunan Taiwan Graduate institute of Medical Sciences Chang Jung Christian University Tainan Taiwan

4,4’-Methylenebis(2-chloroaniline) (MBOCA) is a probable human carcinogen. Few studies have been performed regarding the genotoxicity of MBCOA, and the MBOCA metabolic pathway is not fully understood. We treated four-week-old iCR male mice weighing 25 - 30 g with MBOCA and observed the effects of MBOCA on the internal organs. it can be concluded that MBOCA is a carcinogen and also affects gene regulation. Oral or topical administration of 50 mg/kg, 100 mg/kg or 200 mg/kg MBOCA resulted in 56% - 81% of mice showing unusual inflammation, degeneration, and dysplasia in kidney, liver, stomach, intestine and urinary bladder based on histology. Furthermore, we investigated the association between oxidative DNA damage and MBOCA exposure by measuring plasma level of 8-hydroxydeoxyguanosine (8-OHdG). The results showed that the MBOCA-treated mice had significantly higher 8-OHdG levels than the control mice. This study confirms that MBOCA is potentially carcinogenic and highly toxic to both animals and humans.

关键词： 4 4’-Methylenebis(2-chloroaniline) MBOCA Transitional Cell Carcinoma of Bladder Oxidative DNA Damage 8-Hydroxydeoxyguanosine (8-OHdG)

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