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Cellular Antioxidant Activity and Peroxidase Inhibition of Infusions from Different Aerial Parts of Cassia occidentalis

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Journal of Biosciences and Medicines 2019年第4期7卷 83-94页

作者： nadege kabamba ngombe Claude Ngolo Ngolo Didi Mana Kialengila Arvy Luzayana Wamba Patricia Mbombo Mungisthi Pascal Tshisekedi Tshibangu Pascal Kalenda T. Dibungi Paul Tshilumbu Kantola Paulin Mutwale Kapepula Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Laboratory of Drug Analysis Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo Laboratory of Experimental and Pharmaceutical Microbiology Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of Congo

Cassia occidentalis L. is widely used in the world in traditional medicine and especially in some African countries for the treatment of various diseases. The aim of this study was to report the microscopic features, the chromatographic fingerprints and the cellular antioxidant activity and the peroxidase inhibition of infusions from different parts of this plant. Microscopically, leaf can be characterized by cells of the spongy mesophyll and parenchyma numerous cluster crystals of calcium oxalate, paracytic stomata, isolated calcium oxalate cluster crystals, covering and glandular trichomes, scalariform vessels, polyedric starch granules, lignified fibers;flowers by abundant covering and glandular trichomes, spirally thickened vessels and associated parenchyma, abundant pollen grains. Seeds were characterized by pluricellular non-glandular trichomes, epidermis of the testa with underlying oil cells, parenchymatous layers of the testa, thicker-walled cells of the endosperm, pollen grain. Phytochemical analysis revealed the presence of phenolic acids, flavonoids, iridoids, tannins and terpenes. TLC fingerprints of different parts were different and characteristic. They showed the presence of glycosylated flavonoids and phenolic acids as main phytochemicals for flowers, leaves and seedpods. ABTS and DPPH assays showed that infusion extracts have the ability to scavenge free radicals connected with their IC50 values ranging from 21.43 ± 1.25 to 566.24 ± 176.7 mg·mL-1. All extracts showed a weaker capacity to scavenge DPPH radical. Aqueous extracts displayed high cellular antioxidant activity at the concentrations range of 1 - 20 μg·mL-1 using LO-12 on monocytes HL 60. Flower and leave extracts showed more efficient effects on extracellular ROS production. Phenolic compounds could be major contributors to antioxidant activity of infusions of Cassia parts. In MPO (Myeloperoxidase) direct technique, all infusion extracts exhibited a dose-dependent inhibitory effect on MPO

关键词： Cassia occidentalis HRP Manalaria Monocytes HL 60 MPO Nutraceutical

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Microscopic Characteristics, Chromatographic Profiles and Inhibition of Peroxidase Activity of the Leaves of Manihot esculentaand Manihot glaziovii, Consumed as Traditional Vegetables

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Journal of Biosciences and Medicines 2021年第9期9卷 59-73页

作者： Paulin Mutwale Kapepula Patricia Mbombo Mungitshi Dieudonné Tshitenge Tshitenge Thierry Franck Dieudonné Mumba Ngoyi Pascal Dibungi T. Kalenda Monique Tits Michel Frédérich nadege kabamba ngombe Ange Mouithys-Mickalad Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Centre for Oxygen Research and Development (CORD) University of Liège Liège Belgium Faculty of Medicine University of Kinshasa Kinshasa Democratic Republic of the Congo Laboratory of Pharmacognosy Center for Interdisciplinary Research on Medicines (CIRM) University of Liège Liège Belgium

Methanolic extracts from the leaves of Manihot esculenta (Two cultivars) and Manihot glaziovii, consumed as traditional vegetables in DR. Congo was chemically characterized by Thin layer Chromatography and High Performance Liquid Chromatography. In vitro biochemical activities of extracts against Radical Oxidative Species (ROS) production were assessed in cellular models, on enzymes, Myeloperoxidase (MPO) and Horseradish Peroxidase (HRP) involved in inflammation. The microscopic analysis of the powder of leaves showed that each species displays specific and discriminating botanical microscopic features. Varieties of M. esculenta had a chemical fingerprint different from M. glaziovii. The majority of compounds were polyphenols, represented mainly by rutin, kaempferol-3-O-rutinoside, amentoflavone, phenolic acids such as gallic acid. All extracts exhibited high cellular antioxidant activity in the range of 0.1 to 10 μg·mL-1 using lucigenin with neutrophils, but a moderate cellular antioxidant activity ranging between 10 and 100 μg·mL-1 with DCFDA on HL60 monocytes. Extracts from Manihot leaves showed a pronounced inhibitory effect on the production of extracellular ROS, on HRP and myeloperoxidase activity. Cellular antioxidant activities, the inhibitory effect on HRP of extracts from M. glaziovii, M. esculenta cultivar Mwambu were significantly higher, but their inhibitory effect on the activity of MPO was lower than those of M. esculenta cultivar TEM 419. The biological activities of Manihot esculenta and Manihot glaziovii were well correlated to their phytochemicals that could justify their traditional use as vegetables, potential functional foods or nutraceutical resources and medicines.

关键词： Horseradish Peroxidase Kahemba Konzo Manihot esculenta Manihot glaziovii Myeloperoxidase Traditional Vegetable

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Peroxidase Inhibition and Antioxidant Activity of Bulk-Marketed Black Tea (Camellia sinensisL.) from the Democratic Republic of the Congo

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Journal of Biosciences and Medicines 2019年第9期7卷 66-80页

作者： nadege ngombe kabamba Joyce Mbuyi Katalayi Jimmy Kabeya Kabengele Grace Nkakapen Djoumbissi Marie Joe Kabongo Kapinga Thé ophile Mbemba Fundu Dieudonné Tshitenge Tshitenge Pascal Dibungi T. Kalenda Paulin Mutwale Kapepula Centre d’Etudes des Substances Naturelles d’Origine Vé gé tale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa The Democratic Republic of the Congo Laboratoire d’Analyses et de Recherche en Alimentation et Nutrition (LARAN) Department of Biology Faculty of Sciences University of Kinshasa Kinshasa The Democratic Republic of the Congo

The widely known leaves of Camellia sinensis (L.) derive the most consumed beverage globally. All over the world, like in the Democratic Republic of the Congo (DR Congo), they are marketed in the form of packaged and in bulk black tea. The aim of this study was to report the microscopic botanical features, the chromatographic fingerprints, the antioxidant activities, and the peroxidase inhibition of bulk-marketed black teas consumed in DR Congo. The microscopic analysis of powders from unpackaged black tea marketed in DR Congo allowed the identification of specific microscopical features, which are characteristic to C. sinensis (L.) such as numerous asterosclerites, numerous fragments of unicellular trichomes;fragments of abaxial epidermis consisting of cells with slightly wavy walls, numerous anomocytic stomata, and the detection of biological contaminants, e.g. the dust mites. TLC and HPLC analysis indicated that flavonoids (hyperoside, rutin) and phenolic acids (chlorogenic acid, gallic acid) were major phenolic compounds, along with other secondary metabolites. Aqueous extracts from all bulk-marketed black teas exhibited good antioxidant activities at the concentrations range of 1 - 20 μg·mL-1 using in vitro ABTS and DPPH assays. These extracts showed equally inhibitory effects on myeloperoxidase (MPO) and horseradish peroxidase (HRP) activities at the concentration ranges of 1 - 20 μg·mL-1 and 20 - 100 μg·mL-1 respectively. Bulk-marketed black teas consumed in DR Congo possess antioxidant and anti-inflammatory properties comparable to packaged Ceylon tea largely marketed. The observed bioactivities support the health benefits of the consumed black tea by Congolese people, despite the storage conditions to be improved in order to preserve its nutritional and therapeutic assets.

关键词： Antioxidant Activity Black Tea Camellia sinensis HRP L-012 Mites Myeloperoxidase

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Chemical Fingerprint and Anti-Sickling Activity of Rosmarinic Acid and Methanolic Extracts from Three Species of Ocimum from DR Congo

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Journal of Biosciences and Medicines 2016年第1期4卷 59-68页

作者： Dorothée D. Tshilanda Paulin K. Mutwale Damase V. N. Onyamboko Philippe B. Babady Philippe V. Tsalu Damien S. T. Tshibangu nadege K. ngombe M. Frederich Koto-te-Nyiwa Ngbolua Pius T. Mpiana Department of Chemistry Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo Centre d’Etudes des Substances Naturelles d’Origine Végétale (CESNOV) Faculty of Pharmaceutical Sciences University of Kinshasa Kinshasa Democratic Republic of the Congo Bila Nutraceuticals Company Dartmouth Canada Laboratory of Pharmacognosy Department of Pharmacy CIRM University of Liège CHU Liège Belgium Department of Biology Faculty of Science University of Kinshasa Kinshasa Democratic Republic of the Congo

The aim of this study was to characterize the polyphenolic composition by determination of chemical fingerprints of Methanolic extracts of Ocimum canum Sims, Ocimum basilicum L. and Ocimum gratissimum L. from Democratic Republic of Congo and to compare their antisickling activity of that of rosmarinic acid, the major compound to those of methanolic extracts. Phytochemical analysis performed by TLC and HPLC analysis, showed that rosmarinic acid is the most abundant phenolic acid in these Ocimum species according to the following order O. basilicum L., O. gratissimum L. and O. canum Sims. Methanolic extracts of these three species and pure rosmarinic acid showed significant antisickling activities with minimal concentration of normalization values of 0.18 ± 0.03, 0.23 ± 0.04, 0.26 ± 0.04 and 0.31 ± 0.05 mg/mL for rosmarinic acid, O. basilicum L., O. gratissimum L. and O. canum Sims methanolic extracts respectively. The antisickling activity order is the same as that of the rosmarinic acid content, indicating that this polyphenolic acid would be among the main active molecules in these extracts.

关键词： Antisickling Chemical Fingerprint O. canum O. basilicum O. gratissimum Rosmarinic Acid

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