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An updated review on pharmacological activities and phytochemical constituents of evening primrose(genus Oenothera)

Asian Pacific Journal of Tropical Biomedicine 2017年第11期7卷 1046-1054页

作者： Rebecca Munir nabil semmar Muhammad Farman Naseem Saud Ahmad Department of Pharmacology University of Health SciencesLahore Pakistan Laboratory of Bioinformatics Biomathematics and Biostatistics Pasteur Institute of Tunis University of Tunis El Manar

Genus Oenothera includes medicinal plants that are distributed throughout the world and are known since ancient times. Popular indications of different species of this genus include treatment of inflammations, diabetes, microbial infections, ulcers, tumors, kidney and liver problems. The plants of this genus are a botanical source for various pharmaceutically active components like sterols, alkaloids, phenolic acids, flavonoids, triterpenoids, saponins, biflavonols and tocopherols. This review article is a compilation of chemical composition and biological activities of the various species of the genus Oenothera.

关键词： Oenothera Medicinal plants Chemical composition Biological activities

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Measurement of total phenolic content and antioxidant activity of aerial parts of medicinal plant Coronopus didymus

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Asian Pacific Journal of Tropical Medicine 2017年第8期10卷 855-862页

作者： Hafiza Noreen nabil semmar Muhammad Farman James S.O.McCullagh Department of Chemistry Quaid-i-Azam UniversityIslamabad 45320Pakistan Institut Supérieur des Sciences Biologiques Appliquées de Tunis Tunisia Chemistry Research Laboratory Department of ChemistryUniversity of OxfordMansfield RoadOxford OX1 3TAUnited Kingdom

Objective: To evaluate the total phenolic content and compare the antioxidant activity of various solvent extracts and fractions from the aerial parts of Coronopus didymus through various assays. Methods: Total phenolic content was determined using the Folin-Ciocalteu assay and the in vitro antioxidant activity of a number of different extracts was investigated in a dose-dependent manner with three different methods: the 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS) and ferric reducing antioxidant power(FRAP) assays. A flavone was isolated from the most active ethanolic extract with high antioxidant activity using size exclusion chromatography. IC_(50) values were calculated for the DPPH and ABTS methods. The FRAP activity was assessed in terms of μM Fe(II) equivalent. Results: The phenolic content was found to be highest in the ethanol extract(CDA Et; 47.8 mM GAE) and the lowest in the dichloromethane extract(CDA DCM; 3.13 mM GAE). The ethanol extract showed high radical scavenging activity towards DPPH and ABTS radicals with IC_(50) values of(7.80 × 10~2) and(4.32 × 10~2) μg/m L, respectively. The most active ethanol extract had a FRAP value of 1 921.7 μM Fe(Ⅱ) equivalent. The isolated flavone F10C(5,7,4'-trihydroxy-3'-methoxy flavone) was far more effective for scavenging free radicals in the DPPH and ABTS assays with IC_(50) of 43.8 and 0.08 μg/m L, than the standard trolox, with IC_(50) values of 97.5 and 21.1 μg/m L, respectively. In addition, the flavone F10C and the standard ascorbic acid had FRAP values of 1 621.7 and 16 038.0 μM Fe(Ⅱ) equivalents, respectively. Conclusions: The total phenolic content of extracts in decreasing order is ethanol extract(CDA Et)> acetone extract(CDA ACE)> phenolic extract(CDA MW)> n-hexane extract(CDA nHX)> chloroform extract(CDA CHL)> dichloromethane extract(CDA DCM). The ordering of extracts in terms of antioxidant activity from highest to lowest is CDA Et> C

关键词： Coronopus didymus 抗氧化剂活动总计酉分的内容缩放排除层析

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