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Influence of BA and IBA or NAA Combinations on Micropropagation of Cryptolepis sanguinolenta

American Journal of Plant Sciences 2016年第3期7卷 572-580页

作者： Maame Adjoa Dwumawa Monney naalamle amissah Essie Blay Crop Science Department University of Ghana Accra Ghana

The study being the first of its kind established an efficient protocol for micropropagation of Cryptolepis sanguinolenta, an important endangered medicinal plant species, used in the treatment of Malaria. For shoot induction, semi hard wood nodal segments were maintained on MS (Murashige and Skoog) nutrient medium supplemented with MS vitamins, 30 g/L sucrose, 3% gelrite and various auxin and cytokinin combinations. Treatments involved 6-benzyladenine (BA) at 0.5, 1.0, 1.5, 2.0, 2.5 or 3.0 mg/L in combination with 0.1 mg/L Indole 3-butyric acid (IBA) or Naphthaleneacetic acid (NAA). Control consisted of hormone free MS medium. BA and IBA combinations were found to be more efficient in shoot regeneration than the BA and NAA combinations. Cultures maintained on MS medium supplemented with 3 mg/L BA, in combination with 0.1 mg/L IBA recorded the highest shoot induction (100%), mean shoot length (1.28 cm) and mean number of nodes per explant (2.5). This, however, did not differ significantly from cultures maintained on 2 or 2.5 mg/L BA and 0.1 mg/L IBA supplemented MS medium. Regenerated shoots were transferred onto different media for root induction. Treatments consisted of full strength MS medium augmented with either 0.5 or 0.1 mg/L IBA, hormone free half strength MS medium and half strength MS medium augmented with 0.01 mg/L IBA. After six weeks of culture, no rooting was recorded in all treatments with the exception of half strength MS medium supplemented with 0.01 mg/L IBA, which recorded 60% rooting. Regenerated plantlets were successfully weaned and established in the greenhouse.

关键词： C. sanguinolenta Micropropagation Plant Growth Regulators

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Asexual Propagation of Sheanut Tree (<i>Vitellaria paradoxa</i>C.F. Gaertn.) Using a Container Layering Technique

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American Journal of Plant Sciences 2013年第9期4卷 1758-1765页

作者： naalamle amissah Brain Akakpo Julius Yeboah Essie Blay Cocoa Research Institute of Ghana (CRIG) Bole Ghana. Department of Crop Science College of Agriculture and Consumer Sciences University of Ghana Accra Ghana

In spite of the economic potential of the sheanut tree (Vitellaria paradoxa), its domestication still has not been achieved due to the long juvenile period of seed propagated plants and the absence of a reliable vegetative propagation method. Three experiments were conducted by using a modified container layering technique to investigate the effects of season (rainy and dry season), light treatments (etiolation and natural light) and indole 3-butyric acid (IBA) on root formation in juvenile and mature sheanut trees. The effect of light treatments on the internal conditions such as level of sugars (soluble, insoluble and total sugars) and total free phenols in layered sheanut shoots was also considered. Rooting was generally lower in the mature trees (27.9%) compared to that in juvenile 4 year-old plants (40.9%). Etiolation increased the levels of total sugars and phenols in shoots of 4-year-old plants and mature fruit bearing trees, but this increase did not significantly enhance rooting in both the juvenile and mature sheanut trees. The container layering technique holds promise as a method for the asexual propagation of sheanut planting material. Roots formed using this method looked healthy and were vertically orientated giving layered shoots a better chance at survival.

关键词： Etiolation Container Layering Technique Indolebutyric Acid Sheanut Tree

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Effect of Indole 3-Butyric Acid and Media Type on Adventitious Root Formation in Sheanut Tree (<i>Vitellaria paradoxa</i>C. F. Gaertn.) Stem Cuttings

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American Journal of Plant Sciences 2014年第3期5卷 313-318页

作者： Daniel Brain Akakpo naalamle amissah Julius Yeboah Essie Blay Cocoa Research Institute of Ghana (CRIG) Bole Ghana Department of Crop Science College of Agriculture and Consumer Sciences University of Ghana Accra Ghana

Shea nuts play an important role in food security for rural folks within sub-Sahara Africa, serving as the main source of income for many people living in Northern Ghana. Unfortunately, the full economic potential of the Sheanut tree has not been fully realized due to the difficulty involved in its domestication. This difficulty in vegetatively propagating sheanut trees has greatly hindered its cultivation and the realization of its true economic potential. Two experiments were conducted to investigate the effects of rooting media and varying indole 3-butyric acid (IBA) concentrations on adventitious root formation in cuttings taken from coppiced sheanut trees. Results indicated that 3000 ppm produced significantly (p 0.05) better rooting (57.5%) than 5000 ppm (30%), 7000 ppm (45.0%) and the control (7.5%). Although the levels of soluble sugars (SS) and total free phenols (TFP) in the cutting were significantly (p 0.05) higher at the end of the experiment (after IBA treatment) compared to the start (prior to IBA treatment), the SS and TFP trends observed did not clearly explain the rooting differences found between the IBA levels investigated. Callus formation was significantly (p 0.05) higher (35.0%) in the control (no IBA). Generally, callus formation decreased with increasing IBA concentration. In the rooting media experiment, rooting was significantly (p 0.05) higher in the rice husk medium (35.0%) compared to that in the palm fiber (18.3%), saw dust (14.1%) and top soil (16.7%) media.

关键词： Coppiced Indole 3-Butyric Acid Sheanut Tree Cuttings Rooting Medium

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