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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

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Biomedical and Environmental Sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi Jin CHEN Xiao Ping HUA Shao Wei LI Feng Yu ZHAO Meng XINg Chen Hao WANg Jie TIAN Feng Yu ZHANg Rui Qing LYU Xiao na HAN Zhi Qiang WANg Yu Xin LI Hong Yi shen Xin Xin MA Xue Jun TIE Yan Qing graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina national Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China national Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1 protein)-conjugated magnetic bead(M1 bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1 beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1,10,and 1 copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results forgens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.839,0.815,and 0.856,respectively(Pg M1 bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

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Achieving structurally stable O3-type layered oxide cathodes through site-specific cation-anion co-substitution for sodium-ion batteries

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Journal of Energy Chemistry 2024年第6期93卷 411-418,I0011页

作者： Yihao shen Chen Cheng Xiao Xia Lei Wang Xi Zhou Pan Zeng Jianrong Zeng lia.g Zhang Institute of Functional nano&Soft Materials(FUNSOM) Soochow UniversitySuzhou 215123JiangsuChina Institute for Advanced Study School of Mechanical EngineeringChengdu UniversityChengdu 610106SichuanChina Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China Shanghai Institute of Applied Physics Chinese Academy of SciencesShanghai 201800China Jiangsu Key Laboratory of Advanced Negative Carbon Technologies Soochow UniversitySuzhou 215123JiangsuChina

O3-type layered oxides have garnered great attention as cathode materials for sodium-ion batteries because of their abundant reserves and high theoretical ***,challenges persist in the form of uncontrollable phase transitions and intricate na^(+)diffusion pathways during cycling,resulting in compromised structural stability and reduced capacity over *** study introduces a special approach employing site-specific Ca/F co-substitution within the layered structure of O_(3)-naNi_(0.5)Mn_(0.5)O_(2) to effectively address these ***,the strategically site-specific doping of Ca into na sites and F into O sites not only expands the na^(+)diffusion pathways but also orchestrates a mild phase transition by suppressing the na^(+)/vacancy ordering and providing strong metal-oxygen bonding strength,*** as-synthesized na_(0.95)Ca_(0.05)Ni_(0.5)Mn_(0.5)O_(1.95)F_(0.05)(NNMO-CaF)exhibits a mild O3→O3+O'3→P3 phase transition with minimized interlayer distance variation,leading to enhanced structural integrity and stability over extended *** a result,NNMO-CaF delivers a high specific capacity of 119.5 mA h g^(-1)at a current density of 120 mA g^(-1)with a capacity retention of 87.1%after 100 *** study presents a promising strategy to mitigate the challenges posed by multiple phase transitions and augment na^(+)diffusion kinetics,thus paving the way for high-performance layered cathode materials in sodium-ion batteries.

关键词： Sodium-ion batteries O3-type layered oxides Site-specific co-doping Phase transition

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Bipolar membrane electrodialysis integrated with in-situ CO_(2)absorption for simulated seawater concentrate utilization,carbon storage and production of sodium carbonate

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Journal of Environmental Sciences 2024年第8期142卷 21-32页

作者： Jingtao Bi Tianyi Chen Yue Xie Ruochen shen Bin Li Mengmeng Sun Xiaofu guo Yingying Zhao School of Chemical Engineering and Technology Hebei University of TechnologyTianjin 300401China Engineering Research Center of Seawater Utilization Technology of Ministry of Education School of Chemical Engineering and TechnologyHebei University of TechnologyTianjin 300401China Hebei Collaborative Innovation Center of Modern Marine Chemical Technology Tianjin 300401China Tianjin Key Laboratory of Chemical Process Safety Tianjin 300130China Shandong Technology Innovation Center of Seawater and Brine Efficient Utilization Weifang 262737China

In the context of carbon capture,utilization,and storage,the high-value utilization of carbon storage presents a significant *** address this challenge,this study employed the bipolar membrane electrodialysis integrated with carbon utilization technology to prepare na_(2)CO_(3)products using simulated seawater concentrate,achieving simultaneous saline wastewater utilization,carbon storage and high-value production of na_(2)CO_(3).The effects of various factors,including concentration of simulated seawater concentrate,current density,CO_(2)aeration rate,and circulating flow rate of alkali chamber,on the quality of na_(2)CO_(3)product,carbon sequestration rate,and energy consumption were *** the optimal condition,the CO_(3)^(2-)concentration in the alkaline chamber reached a maximum of 0.817 mol/L with 98 mol%*** resulting carbon fixation rate was 70.50%,with energy consumption for carbon sequestration and product production of 5.7 k Whr/m^(3)CO_(2)and1237.8 k Whr/ton na_(2)CO_(3),*** coupling design provides a triple-win outcome promoting waste reduction and efficient utilization of resources.

关键词： Bipolar membrane electrodialysis(BMED) Carbon capture Utilization and storage(CCUS) Seawater concentrate Sodium carbonate

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COX6B2在胃癌组织中高表达并影响患者的远期预后:基于抑制p53信号调控胃癌细胞的增殖及细胞周期

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南方医科大学学报 2024年第2期44卷 289-297页

作者：沈梦迪赵娜邓晓晶邓敏蚌埠医学院第一附属医院消化内科安徽蚌埠233004 蚌埠医学院安徽省生化药物研究工程中心安徽蚌埠233030

目的分析COX6B2在胃癌组织中的表达对患者远期预后的影响及其作用机制。方法基于公共数据库、患者病历资料分析COX6B2在胃癌和癌旁组织中表达量及其对患者预后的影响;富集分析COX6B2在胃癌中可能发挥的作用;应用慢病毒转染技术干预COX6B... 详细信息

目的分析COX6B2在胃癌组织中的表达对患者远期预后的影响及其作用机制。方法基于公共数据库、患者病历资料分析COX6B2在胃癌和癌旁组织中表达量及其对患者预后的影响;富集分析COX6B2在胃癌中可能发挥的作用;应用慢病毒转染技术干预COX6B2的表达;通过CCK-8、流式细胞术及免疫印迹实验验证其生物学功能。结果TCgA数据库、免疫组化、免疫印迹和荧光定量PCR检测显示,COX6B2在胃癌组织中高表达(Pg/L及CA19-9≥37 kU/L均是影响患者术后5年生存率的独立危险因素(PgO及KEgg富集结果显示,COX6B2主要参与细胞周期的调控等;CCK-8和流式检测显示,上调COX6B2促进细胞增殖,通过增加g1/S期细胞比例调控细胞周期(P<0.05)。免疫印迹结果显示,COX6B2抑制胃癌细胞中p53和p21的表达(P<0.05)。结论COX6B2在胃癌组织中高表达且影响患者远期预后,其可能通过调控细胞周期而影响胃癌恶性增殖的过程。

关键词：胃癌 COX6B2 细胞周期细胞增殖远期预后 p53

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Two sex pheromone receptors for sexual communication in the American cockroach

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Science China(Life Sciences) 2024年第7期67卷 1455-1467页

作者： na Li Renke Dong Huanchao Zeng Yan Zhang Run Huang Wei Liu Fengming Cao Jincong Yu Mingtao liao Jingyou Chen Wenlei Zhang Zejian Huang Jiahui Wang Li Li shen Zhu Danyan Huang Zining Li Xiaoshuai Zhang Dongwei Yuan nan Chen Yonglia.g Fan guirong Wang Coby Schal Yufeng Pan sheng Li guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhou 510631China guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhou 514589China Lingnan guangdong Laboratory of Modern Agriculture Genome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518000China State Key Laboratory of Crop Stress Biology for Arid Areas and Key Laboratory of Integrated Pest Management on the Loess Plateau of Ministry of AgricultureNorthwest A&F UniversityYangling 712100China Department of Entomology and Plant Pathology North Carolina State UniversityRaleigh 27695USA The Key Laboratory of Developmental genes and Human Disease School of Life Science and TechnologySoutheast UniversityNanjing 210096China

Volatile sex pheromones are vital for sexual communication between males and *** of the American cockroach,Periplaneta americana,produce and emit two sex pheromone components,periplanone-A(PA)and periplanone-B(PB).Although PB is the major sex attractant and can attract males,how it interacts with PA in regulating sexual behaviors is still *** this study,we found that in male cockroaches,PA counteracted PB *** identified two odorant receptors(ORs),OR53 and OR100,as PB/PA and PA receptors,***53 and OR100 were predominantly expressed in the antennae of sexually mature males,and their expression levels were regulated by the sex differentiation pathway and nutrition-responsive *** localization of OR53 and OR100 in male antennae further revealed that two types of sensilla coordinate a complex two-pheromone-two-receptor pathway in regulating cockroach sexual *** findings indicate distinct functions of the two sex pheromone components,identify their receptors and possible regulatory mechanisms underlying the male-specific and age-dependent sexual behaviors,and can guide novel strategies for pest management.

关键词： sex pheromone odorant receptor sexual attraction sex differentiation cockroach

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某型集装箱船舱壁变形原因分析及工艺优化

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船海工程 2024年第S1期53卷 113-116页

作者：沈通孙丽娜中国船级社江苏分社南京210011 中华人民共和国南京海事局南京210011

某国际航行集装箱船在建造过程中货舱后端壁产生褶皱变形,针对此问题进行强度校核和工艺流程两个方面的原因剖析。通过船级社规范计算和有限元校核的方法,对产生变形的舱壁在下水、强度试验等各个阶段进行结构强度分析,再与同型船施工... 详细信息

某国际航行集装箱船在建造过程中货舱后端壁产生褶皱变形,针对此问题进行强度校核和工艺流程两个方面的原因剖析。通过船级社规范计算和有限元校核的方法,对产生变形的舱壁在下水、强度试验等各个阶段进行结构强度分析,再与同型船施工顺序、建造工艺进行对比,得出舱壁变形是由下水和主机吊装时机不合理导致,最终提出工艺改进优化措施,有效解决了后续船舱壁变形的问题。

关键词：舱壁变形大开口结构强度校核下水工艺

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Rapid lithium-ion insertion/extraction and migration behavior of na_(2)WO_(4)-encapsulated lithium-rich layered oxide cathode

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Rare Metals 2024年第8期43卷 3647-3660页

作者： Yu-shen Zhao Fan-Fan Wang Jian-Cang Wang Peng-Fei Wang Ting-Feng Yi Jun-Hong Zhang School of Materials Science and Engineering Northeastern UniversityShenyangShenyang 110819China Key Laboratory of Dielectric and Electrolyte Functional Material Hebei Province School of Resources and MaterialsNortheastern University at QinhuangdaoQinhuangdao 066004China Shandong Provincial Key Laboratory of Chemical Energy Storage and Novel Cell Technology College of Chemistry and Chemical EngineeringLiaocheng UniversityLiaocheng 252059China

Due to the low cost,high working voltage and high storage capacity,Li-rich Mn-based layered compounds show promise as the cathode materials for lithiumion batteries(LIBs).However,the side reactions at the solid-liquid interface of the cathode will lead to rapid capacity decay and inferior rate ***,this article proposes a liquid-phase dispersion strategy to introduce a na_(2)WO_(4)layer on the Li_(1.2)Ni_(0.13)-Co_(0.13)Mn_(0.54)O_(2) cathode,which can reduce the side effects between raw materials and electrolyte and promote the insertion/extraction rate of Li^(+),thus enhancing the material stability and rate *** a result,the capacity retention rate is 96.9%after 200 cycles under ***,the capacities are 177.5,149.5,111.1 and58.3 mAh·g^(-1)at 1C,2C,5C and 10C,implying a superior fast charging *** exceptional performance can be ascribed to both the increased conductivity and enhanced structural stability of the cathode ***'s more,based on the investigation of ion insertion/extraction behavior in electrode materials and the ion migration kinetics in the electrolyte,this study suggests that coating Li-rich Mn-based materials with na_(2)WO_(4)can be a promising strategy to improve their performance in LIBs.

关键词： Li-ion battery Coating Rate performance Li_(1.2)Ni_(0.13)Co_(0.13)Mn_(0.54)O_(2) Reaction kinetics

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Dual-Functional Electrode Promoting Dendrite-Free and CO_(2) Utilization Enabled High-Reversible Symmetric na-CO_(2) Batteries

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Energy & Environmental Materials 2024年第3期7卷 123-132页

作者： Changfan Xu Jiajia Qiu Yulian Dong Yuelia.g Li Yonglong shen Huaping Zhao Ute Kaiser guosheng Shao Yong Lei Fachgebiet Angewandte nanophysik Institut für Physik&IMN MacroNanoTechnische Universität Ilmenau 98693Ilmenau Germany Central Facility for Electron Microscopy Electron Microscopy Group of Materials ScienceUlm UniversityUlm 89081Germany School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001China

Sodium-carbon dioxide(na-CO_(2))batteries are regarded as promising energy storage technologies because of their impressive theoretical energy density and CO_(2)reutilization,but their practical applications are restricted by uncontrollable sodium dendrite growth and poor electrochemical kinetics of CO_(2)*** suitable multifunctional electrodes for dendritefree anodes and kinetics-enhanced CO_(2)cathodes is considered one of the most important ways to advance the practical application of na-CO_(2)***,RuO2 nanoparticles encapsulated in carbon paper(RuCP)are rationally designed and employed as both na anode host and CO_(2)cathode in na-CO_(2)*** outstanding sodiophilicity and high catalytic activity of RuCP electrodes can simultaneously contribute to homogenous na+distribution and dendrite-free sodium structure at the anode,as well as strengthen discharge and charge kinetics at the *** morphological evolution confirmed the uniform deposition of na on RuCP anode with dense and flat interfaces,delivering enhanced Coulombic efficiency of 99.5%and cycling stability near 1500 ***,na-CO_(2)batteries with RuCP cathode demonstrated excellent cycling stability(>350 cycles).Significantly,implementation of a dendrite-free RuCP@na anode and catalytic-site-rich RuCP cathode allowed for the construction of a symmetric na-CO_(2)battery with long-duration cyclability,offering inspiration for extensive practical uses of na-CO_(2)batteries.

关键词： CO_(2)cathode dendrite free electrocatalysis na metal anode symmetric CO_(2)batteries

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Mechanism of imipenem-induced mental disorder: A meta-analysis

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World Journal of Psychiatry 2024年第10期14卷 1583-1591页

作者： Zhou-Hong Zhan Jia-lia.g Wang Li-Hong Wang nan-nan shen Xin-Wen Liu Yan-na Yu Fu-Rong Liu Department of Clinical Pharmacy Affiliated Hospital of Shaoxing UniversityShaoxing 312000Zhejiang ProvinceChina

BACKgROUND Imipenem is a highly effective carbapenem antibiotic,which is widely used in the treatment of many serious bacterial *** the same time,it can also cause some adverse reactions,mental abnormalities are the most concerned central nervous system adverse *** patients respond differently to imipenem,and the effect of imipenem on psychiatric disorders is ***,meta-analysis summarizing the results of multiple previous studies can provide stronger evidence support for clinical guidelines to guide clinical rational use of imipenem to minimize *** reviewing the literature published between 2003 and 2017,seven controlled trials with a total of 550 patients were included,with 273 and 277 patients in the control and experimental groups,*** sample size of the study ranged from a minimum of 30 cases to a maximum of 61 *** in the experimental group were treated with imipenem while the control group was treated with conventional ***-analysis showed that the incidence of mental disorders in the experimental group was higher than that in the control group(odds ratio=3.66,95%confidence interval:1.11-12.11,P=0.030);however,there was no significant difference in the incidence of adverse reactions between the two groups(odds ratio=0.05,95%confidence interval:0.00 to 0.10,P=0.060).Funnel diagrams showed that the scattered points of each study were symmetrical and distributed in an inverted funnel shape;therefore,there was no publication *** Imipenem can cause mental disorders in ***,the low quality of the included literature may have affected the final ***,it is necessary to conduct a high-quality randomized controlled study with multiple samples to further confirm the mechanism of imipenem-induced mental disorders and provide effective guidance for clinical treatment.

关键词： Imipenem Psychosis Drug mechanism Meta analysis Document resource quality

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信息共享下的港口泊位调度模式与方法

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系统管理学报 2024年第3期33卷 578-588页

作者：赵娅彤李娜沈立新大连海事大学交通运输工程学院辽宁大连116026 大连海事大学航运经济管理学院辽宁大连116026

区块链等信息技术的发展为上下游港口间的信息共享提供了条件,促进了港口间的协作运营。针对港口间信息不通畅、泊位计划受扰动等问题,提出上下游港口间的信息共享机制,设计了各参与方共享港口作业数据的内容、权限与更新流程,重点研究... 详细信息

区块链等信息技术的发展为上下游港口间的信息共享提供了条件,促进了港口间的协作运营。针对港口间信息不通畅、泊位计划受扰动等问题,提出上下游港口间的信息共享机制,设计了各参与方共享港口作业数据的内容、权限与更新流程,重点研究了信息共享下的动态泊位调度模式和方法,讨论了信息共享的策略、参与度以及时效性等因素对所有船舶在港周转时间的影响。数值实验验证了信息共享的效果,成员参与度、信息的时效性对提升码头服务水平具有重要作用。上游港口即使少部分信息共享,也可提升下游港口的泊位利用效率,缩短船舶在港周转时间,降低不确定性因素对港口泊位计划的干扰,减少上游港口扰动带来的蔓延性影响。

关键词：信息共享泊位调度合作运作模式

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