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沙地云杉种子内参基因的筛选与验证

分子植物育种 2024年第8期22卷 2600-2610页

作者：李佳琪白玉娥吉仁花哈布尔娜苏勒玛内蒙古农业大学林学院呼和浩特010000 内蒙古蒙荣园林绿化工程有限公司呼和浩特010000

为筛选出沙地云杉(Picea mongolica)种子成熟过程中均可稳定表达的内参基因,本研究以沙地云杉种子不同发育时期的样本为研究对象,采用RT-qPCR技术,检测沙地云杉种子转录组数据库中GAPDH、EF1α、CYC、Actin等8个候选内参基因在种子发育... 详细信息

为筛选出沙地云杉(Picea mongolica)种子成熟过程中均可稳定表达的内参基因,本研究以沙地云杉种子不同发育时期的样本为研究对象,采用RT-qPCR技术,检测沙地云杉种子转录组数据库中GAPDH、EF1α、CYC、Actin等8个候选内参基因在种子发育过程中呈现的综合表达水平,并通过内参基因分析软件(geNorm,NormFinder,BestKeeper)对其稳定性统一评估。结果表明,候选内参基因中稳定性最好的是CYC、EF1α和GAPDH。分别以CYC、EF1α和GAPDH作为内参基因对沙地云杉胚胎发育关键调控基因LEA1(late embryogenesis abundant protein)和WOX9 (WUSCHEL related homebox)进行了定量分析,其表达趋势基本一致。本研究结果将对沙地云杉及近缘物种的分子育种研究提供参考。

关键词：沙地云杉(Picea mongolica) 内参基因 RT-qPCR 基因表达

High-performance colorful semitransparent perovskite solar cells with phase-compensated microcavities

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nano Research 2018年第5期11卷 2553-2561页

作者： Kyu-Tae Lee Ji-Yun Jang na Young ha Soonil Lee Hui Joon Park Department of Materials Science and Engineering University of Illinois Urbana Illinois 61801 USA Department of EnergySystems Research Ajou University Suwon 16499 Republic of Korea Department of Electrical and Computer Engineering Ajou University Suwon 16499 Republic of Korea

We demonstrate highly efficient multi-colored semitransparent perovskite solar cells that can create high angular tolerant controllable transmissive colors up to 60~, based on phase-compensated microcavities. The efficiency of the semitransparent colors was improved by impedance matching, which was enabled by placing a dielectric functional layer on top of traditional optical microcavities, with negligible influence on color pureness. The vast majority of the visible part of solar radiation is efficiently utilized for solar energy harvesting, achieving 10.47%, 10.66%, and 11.18% of efficiency for red, green, and blue （RGB） colored solar cells, respective136 while a very small proportion of the visible solar spectrum is used for structural coloration that can be readily tuned by altering the cavity medium thickness. The approach described herein can be suitable for a variety of applications such as display systems with ultra-low power consumption, highly efficient colorful solar panels, low-power wearable electronics, and energy-efficient optoelectronics.

关键词： perovskite solar cells semitransparency color filters optical cavities

鄂温克族、达斡尔族及蒙古族布里亚特部成人的体成分特征与肥胖

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解剖学报 2024年第3期55卷 356-362页

作者：希露格于会新廉祎伊拉娜韩瑗李咏兰内蒙古师范大学生命科学与技术学院内蒙古师范大学民族学人类学学院内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室呼和浩特010022

目的探讨鄂温克族、达斡尔族及蒙古族布里亚特部3个族群成人体成分特点以及肥胖状况。方法采用生物电阻抗法,对鄂温克族245例(男124,女121)、达斡尔族207例(男90,女117)、蒙古族布里亚特部181例(男74,女107)成人进行18项体成分测量。运... 详细信息

目的探讨鄂温克族、达斡尔族及蒙古族布里亚特部3个族群成人体成分特点以及肥胖状况。方法采用生物电阻抗法,对鄂温克族245例(男124,女121)、达斡尔族207例(男90,女117)、蒙古族布里亚特部181例(男74,女107)成人进行18项体成分测量。运用Excel 2016、SPSS 24.0统计学软件进行数据处理。结果相关分析结果表明,3个族群男性、女性内脏脂肪等级与年龄成显著正相关(P鄂温克族>达斡尔族。聚类分析表明,鄂温克族、达斡尔族及蒙古族布里亚特部与北方族群距离较近,与南方族群较远。结论鄂温克族与蒙古族布里亚特部多数体成分指标值最为接近,3个族群均具有较为严重的肥胖问题。

关键词：鄂温克族达斡尔族蒙古族布里亚特部体成分生物电阻抗法成人

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生物技术在沙化土壤修复中的应用

Solvothermal Synthesis and Characterization of Sheet Structure Containing Adamantane [Hg_2Sn_2S_(10)]^(8-) Anions

中国科技纵横 2024年第5期 41-43页

作者：崔嵬唐娜吉木色乌达巴拉内蒙古绿创环保科技有限公司内蒙古呼和浩特 010010 呼和浩特市生态环境监控中心内蒙古呼和浩特 010010

过去由于耕地过度开垦、草场过载放牧等原因,我国土壤出现严重的沙化、退化现象,变得更加贫瘠.土壤生产力下降的同时,水土严重流失,不仅不利于农业生产,而且会引发沙尘暴等灾害,危害人们的正常生活.在当前可持续发展理念下,为防止生态... 详细信息

过去由于耕地过度开垦、草场过载放牧等原因,我国土壤出现严重的沙化、退化现象,变得更加贫瘠.土壤生产力下降的同时,水土严重流失,不仅不利于农业生产,而且会引发沙尘暴等灾害,危害人们的正常生活.在当前可持续发展理念下,为防止生态环境进一步恶化,保护我国农业生产有序推进,需加大力度开展沙化土壤修复工作,将其进行恢复和重建.基于此,分析沙化土壤形成的原因及危害,并探讨沙化土壤中生物技术的应用.

关键词：生物技术沙化土壤成因危害修复

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Chemical Research in Chinese Universities 2011年第6期27卷 915-918页

作者： BAIYIN Meng-he LIANG Feng-ying XU Xiao-qing TA na na Mi-la BAI Wu-yun BAO Yong-sheng College of Chemistry and Environmental Science Inner Mongolia Normal University Huhhot 010022 P. R. China Inner Mongolian Key laboratory for Physics and Chemistry of Functional Materials Inner Mongolia Normal University Huhhot 010022 P. R. China

Sheet Rb2HgSnS4 was synthesized solvothermally and characterized by X-ray single crystal diffraction. The compound is comprised of sheets with admantane-like [Hg2Sn2S10]8– units. The crystals belong to the space group C2/c, with the unit cell parameters a=1.1063（2） nm, b=1.1071（2） nm, c=1.5741（3） nm, a=90o, β=100.13（3）o, γ=90o. A reflectance spectroscopy study reveals the nature of the semiconductor with an energy of 2.1 eV for the compound.

关键词： Solvothermal synthesis Quaternary thiostannate Sheet structure

酚酸-透明质酸钠接枝物的制备及体外抗氧化研究

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生物化学与生物物理进展 2024年第8期51卷 1950-1962页

作者：张晓月王晓娜蒋敏韩婷婷龚劲松李清娜杨素珍史劲松江南大学生命科学与健康工程学院无锡214122 山东福瑞达生物股份有限公司济南250101

目的以透明质酸钠(sodium hyaluronate,ha)为研究对象,利用酚酸对其修饰改性以期获得抗氧化活性更好甚至新活性的分子结构。方法采用自由基介导的接枝方法制备5种不同的酚酸-透明质酸钠接枝物,选出接枝度最高的接枝物进行合成条件优化... 详细信息

目的以透明质酸钠(sodium hyaluronate,ha)为研究对象,利用酚酸对其修饰改性以期获得抗氧化活性更好甚至新活性的分子结构。方法采用自由基介导的接枝方法制备5种不同的酚酸-透明质酸钠接枝物,选出接枝度最高的接枝物进行合成条件优化。通过红外光谱(infrared spectroscopy,IR)、紫外光谱(ultraviolet spectrum,UV)、核磁共振氢谱(^(1)H NMR spectroscopy,^(1)H NMR)、场发射扫描电镜(field emission scanning electron microscopy,FESEM)、热重分析法(thermogravimetry analysis,TGA)对接枝物进行表征,通过测定接枝物对1,1-二苯基-2-三硝基苯肼(DPPH·)、2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS^(+)·)及超氧阴离子自由基(O^(2-)·)清除能力反应出接枝物的体外抗氧化能力。结果在相同条件下,5种酚酸-透明质酸钠接枝物种中接枝度最高的是阿魏酸-透明质酸钠接枝物(ferulic acid-sodium hyaluronate FA-ha),对FA-ha合成条件行进优化,得到最高的接枝度为(16.59±0.31)mg/g。通过IR、UV、^(1)H NMR、FESEM、TGA验证了接枝物的形成,推测可能形成了酯键。分子质量检测结果表明,通过本方法得到的接枝物的分子质量有小幅下降但分子分布均一性提高,平均分子质量为31.5 ku。FESEM结果显示,接枝物呈现紧密相连的片状结构,表面相对光滑。热重分析(TGA)结果表明,接枝物的热稳定性降低。体外抗氧化实验结果表明,在0.25~10 g/L浓度范围内,FA-ha对DPPH·、ABTS^(+)·和O^(2-)·的最大清除率分别为(83.76±4.86)%、(76.95±5.06)%和(83.08±2.51)%,高于原ha及FA。结论自由基接枝法成功将FA与ha接枝在一起,且接枝后的FA-ha具有更好的体外抗氧化活性,为酚酸-ha接枝物的深入研究和开发应用提供了理论依据。

关键词：透明质酸钠酚酸阿魏酸自由基接枝抗氧化

Effect of Alkali Stress on Soluble Sugar, Antioxidant Enzymes and Yield of Oat

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Journal of Integrative Agriculture 2013年第8期12卷 1441-1449页

作者： BAI Jian-hui LIU Jing-hui ZhaNG na YANG Jun-heng SA Ru-la WU lan Science Innovation Team of Oat Inner Mongolia Agricultural University College of Foreign languages Inner Mongolia Agricultural University

Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were：（i） low concentrations of alkali stress （25 and 50 mmol L^-1） significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde （MDA） content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase （SOD）, and peroxidase （POD） activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; （ii） soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; （iii） alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; （iv） under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress,

关键词： oat alkali stress MDA soluble sugar antioxidant enzyme yield

稳定期COPD患者的能量摄入现状及影响因素分析

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护理学报 2024年第5期31卷 12-16页

作者：黄鑫余丽君张二明哈丽娜中国医学科学院北京协和医学院护理学院北京100144 北京大学首钢医院呼吸与危重症医学科北京100044

目的调查稳定期慢性阻塞性肺疾病(COPD)患者的能量摄入状况,并分析其影响因素。方法调查北京某三级医院及其医疗联合体中所包括的社区卫生服务中心呼吸内科门诊就诊的COPD患者,使用一般资料调查表、3×24 h膳食回顾法进行调查,膳食摄入... 详细信息

目的调查稳定期慢性阻塞性肺疾病(COPD)患者的能量摄入状况,并分析其影响因素。方法调查北京某三级医院及其医疗联合体中所包括的社区卫生服务中心呼吸内科门诊就诊的COPD患者,使用一般资料调查表、3×24 h膳食回顾法进行调查,膳食摄入数据通过营养计算软件进行处理,计算患者平均每日的能量摄入情况,并进行单因素和多元Logistic回归分析,分析稳定期COPD患者能量摄入的影响因素。结果(1)130例稳定期COPD患者中,50.8%的患者能量摄入达到推荐标准,29.2%的患者低于推荐标准,20.0%的患者高于推荐标准;其中,蛋白质、脂肪、碳水化合物供能比例达到推荐标准的患者分别占比36.9%、38.5%、39.2%,低于推荐标准者分别占58.5%、11.5%、35.4%,高于推荐标准者分别占4.6%、50.0%、25.4%。(2)能量摄入与患者的性别、是否单身、月收入水平、每日进餐次数以及疾病因素的影响有关。结论49.2%的稳定期COPD患者的能量摄入不符合推荐标准,蛋白质供能比例低于推荐标准者占58.5%,脂肪供能比例高于推荐标准者占50.0%,有待改善;女性、单身、月收入水平低、每日进食2餐、受到疾病影响是患者发生能量摄入不达标的高危因素,可针对此制定个体化饮食干预方案。

关键词： COPD,稳定期能量摄入影响因素

Application of powdered activated carbon coating to fabrics in a hybrid filter to enhance mercury removal

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Journal of Environmental Sciences 2019年第6期31卷 58-65页

作者： Jin-Ho Sung Seung-Ki Back Eun-Song Lee ha-na Jang Yong-Chil Seo Youn-Suk Kang Myong-Hwa Lee Department of Environmental Engineering Yonsei University J-E Tech Co. Ltd Department of Environmental Engineering Kangwon National University

Elemental mercury(Hg0) is predominant constituent of flue gas emitted from coal-fired power plants. Adsorption has been considered the best available technology for removal of Hg0 from flue gas. However, adsorbent injection increases the amount of ash generated. In the present study, powdered activated carbon(PAC) was coated on polytetrafluoroethylene/glass fiber filters to increase Hg0 removal while concurrently reducing the amount of ash generated. The optimal PAC coating rate was determined in laboratory experiments to ensure better Hg0 removal with low pressure drop. When PAC of particle size less than 45 μm was used, and the areal density was 50 g/m2, the pressure drop remained under 30 Pa while the Hg0 removal efficiency increased to 15.8% from4.3%. The Hg0 removal efficiency also increased with decrease in filtration velocity. The optimal PAC coating rate was applied on a hybrid filter(HF), which was combined with a bag filter and an electrostatic precipitator in a single chamber. Originally designed to remove fine particulates matter, it was retrofitted to the flue gas control device for simultaneous Hg0 removal. By employing the PAC coating, the Hg removal efficiency of the HF increased to 79.79% from 66.35%. Also, a temporary reduction in Hg removal was seen but this was resolved following a cleaning cycle in which the dust layer was removed.

关键词： Mercury Hybrid filter Activated carbon Coating filter Coal-fired power plant