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Improving the electrochemical stability of AZ31 Mg alloy in a 3.5wt.%naCl solution via the surface functionalization of plasma electrolytic oxidation coating

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Journal of Magnesium and Alloys 2022年第5期10卷 1311-1325页

作者： Mosab Kaseem Tehseen Zehra Burak Dikici Ali Dafali Hae Woong Yang Young Gun ko Department of nanotechnology and Advanced Materials Engineering Sejong UniversitySeoul 05006Republic of Korea Department of Metallurgical and Materials Engineering Ataturk UniversityErzurum 25240Turkey Faculty of Sciences Oujda Department of ChemistryMohamed the First UniversityOujdaMorocco Pohang Institute of Metal Industry Advancement Pohang 37666Republic of Korea Materials Electrochemistry Group School of Materials Science and EngineeringYeungnam UniversityGyeongsan 38541Republic of Korea

The unique interactions between hexadecanoic acid(HA)and albumin(ALB)molecules on the surface of the porous layer of AZ31 Mg alloy were exploited to fabricate a novel hybrid composite film with excellent electrochemical stability in a 3.5 wt.%na Cl ***,the inorganic layer(IL)obtained by plasma electrolytic oxidation of AZ31 Mg alloy in an alkaline-phosphate-WO_(3)electrolyte was soaked in an organic solution composed of ALB and HA for 10 and 24 h at 60℃.Although albumin and HA may coexist on the same surface of IL,the higher reactivity of ALB molecules would prevent the formation of a thick layer of *** donor-acceptor complexes formed due to the unique interactions between ALB and/or HA and IL surface would reduce the area exposed to the corrosive species which in turn would efficiently protect the substrate from *** porous structure of the IL would provide preferable sites for the physical and chemical locking triggered by charge-transfer phenomena,leading to the inhomogeneous nucleation and crystal growth of a flowery flakes-like organic *** calculations were performed to reveal the primary bonding modes between the ALB,HA,and IL and to assess the mechanistic insights into the formation of such novel hybrid composites.

关键词： Hybrid composite Coordination bond Albumin Fatty acid Porous layer Corrosion Theoretical approach

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OsSAUR55通过GA途径调控水稻株型

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华北农学报 2024年第2期39卷 106-115页

作者：浦娜高巧丽王慧刘金朝陈嘉玲罗增铜肖武名陈淳华南农业大学国家植物航天育种工程技术研究中心广东广州510642 广州市农业科学研究院广东广州510338

以Francis背景下通过CRISPR/Cas9技术获得的敲除突变体ossaur55-1、ossaur55-2为对象,研究其是否影响水稻株型、叶色、单株穗质量、结实率等农艺性状的变化,探究基因OsSAUR55是否通过参与GA途径调控水稻株型,为相关激素途径调控水稻株... 详细信息

以Francis背景下通过CRISPR/Cas9技术获得的敲除突变体ossaur55-1、ossaur55-2为对象,研究其是否影响水稻株型、叶色、单株穗质量、结实率等农艺性状的变化,探究基因OsSAUR55是否通过参与GA途径调控水稻株型,为相关激素途径调控水稻株型提供理论依据。调查野生型(WT)与敲除突变体成株期的主要农艺性状,包括株高、穗长、单株穗质量、十粒长、十粒宽、剑叶长、剑叶宽等;利用叶绿素计和光合速率测定仪测定野生型和突变体齐穗期剑叶的叶绿素含量和净光合速率;通过实时荧光定量PCR技术(qPCR)测定基因OsSAUR55在水稻种子萌发的不同时间点和不同组织中的表达情况;通过原核表达法观察蛋白OsSAUR55的亚细胞定位情况;采用液相色谱-串联质谱法(LC-MS/MS)测定野生型和突变体的内源激素(GA和IAA)含量;进一步分析GA途径相关基因的表达情况。结果显示,在成株期,突变体ossaur55-1和ossaur55-2都有显著的表型变化,其中突变体的株高比野生型明显变矮、叶色更绿、叶绿素含量增加、光合作用效率降低、结实率降低、千粒质量降低、单株穗质量降低;定量结果显示,OsSAUR55在胚芽中表达量最高,在剑叶中基本不表达;共聚焦结果显示,OsSAUR55可能定位于细胞膜和细胞核;激素检测结果表明,突变体内源激素GA4的含量显著降低;基因的表达量分析发现,突变体中GA合成途径中基因KAO、GA13ox1、GA20ox1、ko1的表达量显著高于野生型,GA代谢途径中GA2ox5、GA2ox8、GA2ox9的表达量也显著高于野生型。初步推测,敲除基因OsSAUR55导致内源GA4含量降低,可能通过GA途径调控水稻株型,进而影响水稻产量。

关键词：水稻 OsSAUR55 株型 CRISPR/Cas9 GA 产量

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Neuroprotective effects of tadalafil on gerbil dopaminergic neurons following cerebral ischemia

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Neural Regeneration Research 2013年第8期8卷 693-701页

作者： Kwang Taek Kim Kyung Jin Chung Han Sae Lee Il Gyu ko Chang Ju Kim Yong Gil na Khae Hawn Kim Department of Urology Gachon University Gil Medical CenterGachon University Department of Physiology College of MedicineKyung Hee University Department of Urology Chungnam National University HospitalChungnam National University School of Medicine

Impairment of dopamine function, which is known to have major effects on behaviors and cognition, is one of the main problems associated with cerebral ischemia. Tadalafil, a long-acting phosphodiesterase type-5 inhibitor, is known to ameliorate neurologic impairment induced by brain injury, but not in dopaminergic regions. We investigated the neuroprotective effects of treatment with tadalafil on cyclic guanosine monophosphate level and dopamine function following cerebral ischemia. Forty adult Mongolian gerbils were randomly and evenly divided into five groups （n = 8 in each group）： Sham-operation group, cerebral ischemia-induced and 0, 0.1, 1, and 10 mg/kg tadalafil-treated groups, respectively. Tadalafil dissolved in distilled water was administered orally for 7 consecutive days, starting 1 day after surgery. Cyclic guanosine monophosphate assay and immunohistochemistry were performed for thyrosine hydroxylase expression and western blot analysis for dopamine D2 receptor expression. A decrease in cyclic guanosine monophosphate level following cerebral ischemia was found with an increase in thyrosine hydroxylase activity and a decrease in dopamine D2 receptor expression in the striatum and substantia nigra region. However, treatment with tadalafil increased cyclic guanosine monophosphate expression, suppressed thyrosine hydroxylase expression and increased dopamine 92 receptor expression in the striatum and substantia nigra region in a dose-dependent manner. Tadalafil might ameliorate cerebral ischemia-induced dopaminergic neuron injury. Therefore, tadalafil has the potential as a new neuroprotective treatment strategy for cerebral ischemic injury.

关键词： neural regeneration brain injury cerebral ischemia Tadalafil phosphodiesterase type-5 inhibitor dopamine dopamine D2 receptor cyclic guanosine monophosphate grants-supported paper photographs-containing paper neuroregneration

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Advantageous mechanochemical synthesis of copper(Ⅰ)selenide semiconductor,characterization,and properties

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Frontiers of Chemical Science and Engineering 2022年第3期16卷 433-442页

作者： Katarína Gáborová Marcela Achimovičová Michal Hegedüs Vladimír Girman Mária Kaňuchová Erika Dutková Institute of Geotechnics Slovak Academy of SciencesKošice04001Slovakia Institute of Metallurgy Faculty of MaterialsMetallurgy and RecyclingTechnical UniversityKošice04201Slovakia Synthon s.r.o.Blansko67801Czech Republic Faculty of Science Pavol JozefŠafárik UniversityKošice04154Slovakia Institute of Earth Resources Faculty of MiningEcologyProcess Control and GeotechnologiesTechnical UniversityKošice04200Slovakia

Copper(I)selenide-nanocrystalline semiconductor was synthesized via one-step mechanochemical synthesis after 5 min milling in a planetary ball *** kinetics of synthesis was followed by X-ray powder diffraction analysis and specific surface area measurements of milled 2Cu/Se *** X-ray diffraction confirmed the orthorhombic crystal structure of Cu_(2)Se with the crystallite size∼25 *** surface chemical structure was studied by X-ray photoelectron spectroscopy,whereby the binding energy of the Cu 2p and Se 3d signals corresponded to Cu^(+)and Se^(2−)oxidation *** electron microscopy revealed agglomerated nanocrystals and confirmed their orthorhombic structure,as *** optical properties were studied utilizing ultraviolet-visible spectroscopy and photoluminescence *** direct bandgap energy 3.7 eV indicated a blue-shift phenomenon due to the quantum size *** type of Cu_(2)Se synthesis can be easily adapted to production dimensions using an industrial vibratory *** advantages of mechanochemical synthesis represent the potential for inexpensive,environmentally-friendly,and waste-free manufacturing of Cu_(2)Se.

关键词： Cu_(2)Se berzelianite nanocrystalline semiconductor mechanochemical synthesis planetary ball mill

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Early extrahepatic recurrence as a pivotal factor for survival after hepatocellular carcinoma resection:A 15-year observational study

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World Journal of Gastroenterology 2022年第36期28卷 5351-5363页

作者： Jae Hyun Yoon Sung Kyu Choi Sung Bum Cho Hee Joon Kim Yang Seok ko Chung Hwan Jun Department of Gastroenterology and Hepatology Chonnam National University Hospital and College of MedicineGwangju 61469South Korea Department of Gastroenterology and Hepatology Hwasun Chonnam National University Hospital and College of MedicineHwasun 58128South Korea Department of Surgery Chonnam National University Hospital and College of MedicineGwangju 61469South Korea Department of Surgery Hwasun Chonnam National University Hospital and College of MedicineHwasun 58128South Korea Department of Internal Medicine Mokpo Hankook HospitalMokpo 58643South Korea

BACKGROUND Surgical resection is one of the most widely used modalities for the treatment of hepatocellular carcinoma(HCC).Early extrahepatic recurrence(EHR)of HCC after surgical resection is considered to be closely associated with poor ***,data regarding risk factors and survival outcomes of early EHR after surgical resection remain *** To investigate the clinical features and risk factors of early EHR and elucidate its association with survival *** From January 2004 to December 2019,we enrolled treatment-naïve patients who were≥18 years and underwent surgical resection for HCC in two tertiary academic *** excluding patients with tumor types other than HCC and/or ineligible data,this retrospective study finally included 779 *** resection of HCC was performed according to the physicians’decisions and the EHR was diagnosed based on contrast-enhanced computed tomography or magnetic resonance imaging,and pathologic confirmation was performed in selected *** Cox regression analysis was performed to identify the variables associated with *** Early EHR within 2 years after surgery was diagnosed in 9.5%of patients during a median followup period of 4.4 *** recurrence-free survival period was 5.2 mo,and the median time to EHR was 8.8 mo in patients with early *** 52.7%of patients with early EHR,EHR occurred as the first recurrence of HCC after surgical *** multivariate analysis,serum albumin100 U/L,surgical margin involvement,venous and/or lymphatic involvement,satellite nodules,tumor necrosis detected by pathology,tumor size≥7 cm,and macrovascular invasion were determined as risk factors associated with early *** sub-categorizing the patients according to the number of risk factors,the rates of both EHR and survival showed a significant correlation with the risk of early ***,multivariate analysis revealed that early E

关键词： Early extrahepatic recurrence Hepatocellular carcinoma Prognosis Surgery Survival

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Unexpected Li displacement and suppressed phase transition enabling highly stabilized oxygen redox in P3-type na layered oxide cathode

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Journal of Energy Chemistry 2023年第10期85卷 144-153,I0006页

作者： Myungeun Choi Hobin Ahn Hyunyoung Park Yongseok Lee Jinho Ahn Bonyoung Ku Junseong Kim Wonseok ko Jungmin Kang Jung-Keun Yoo Duho Kim Jongsoon Kim Department of Energy Science Sungkyunkwan UniversitySuwon 16419Republic of Korea SKKU Institute of Energy Science and Technology(SIEST) Sungkyunkwan UniversitySuwon 16419Republic of Korea Carbon Composites Department Korea Institute of Materials Science(KIMS)Changwon 51508Republic of Korea Department of Mechanical Engineering(Integrated Engineering Program) Kyung Hee University1732Deogyeong-daeroGiheung-guYongin-siGyeonggi-do 17104Republic of Korea Department of KHU-KIST Convergence Science and Technology Kyung Hee University23Kyunghee-daero Dongdaemun-guSeoul 02447Republic of Korea

Oxygen redox is considered a new paradigm for increasing the practical capacity and energy density of the layered oxide cathodes for na-ion batteries. However, severe local structural changes and phase transitions during anionic redox reactions lead to poor electrochemical performance with sluggish ***, we propose a synergy of Li-Cu cations in harnessing the full potential of oxygen redox, through Li displacement and suppressed phase transition in P3-type layered oxide cathode. P3-type na_(0.7)[Li_(0.1)Cu_(0.2)Mn_(0.7)]O_(2) cathode delivers a large specific capacity of ~212 mA h g^(-1)at 15 mA g^(-1). The discharge capacity is maintained up to ~90% of the initial capacity after 100 cycles, with stable occurrence of the oxygen redox in the high-voltage region. Through advanced experimental analyses and first-principles calculations, it is confirmed that a stepwise redox reaction based on Cu and O ions occurs for the charge-compensation mechanism upon charging. Based on a concrete understanding of the reaction mechanism, the Li displacement by the synergy of Li-Cu cations plays a crucial role in suppressing the structural change of the P3-type layered material under the oxygen redox reaction, and it is expected to be an effective strategy for stabilizing the oxygen redox in the layered oxides of na-ion batteries.

关键词： Layered oxide cathode Oxygen redox reaction Structural stability Li displacement No phase transition

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Layered alkali titanates(A_(2)Ti_(n)O_(2n+1)):possible uses for energy/environment issues

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Frontiers in Energy 2021年第3期15卷 631-655页

作者： Taya(ko)SAOTHAYANUN Thipwipa(Tip)SIRInakoRN Makoto OGAWA School of Energy Science and Engineering Vidyasirimedhi Institute of Science and Technology(VISTEC)WangchanRayong 21210Thailand School of Molecular Science and Engineering Vidyasirimedhi Institute of Science and Technology(VISTEC)WangchanRayong 21210Thailand

Uses of layered alkali titanates(A_(2)Ti_(n)O_(2n+1);na_(2)Ti_(3)O_(7),K_(2)Ti_(4)O_(9),and Cs_(2)Ti_(5)O_(11))for energy and environmental issues are *** alkali titanates of various structural types and compositions are regarded as a class of nanostructured materials based on titanium oxide *** compared with commonly known titanium dioxides(anatase and rutile),materials design based on layered alkali titanates is quite versatile due to the unique structure(nanosheet)and morphological characters(anisotropic particle shape).Recent development of various synthetic methods(solid-state reaction,flux method,and hydrothermal reaction)for controlling the particle shape and size of layered alkali titanates are *** ion exchange ability of layered alkali titanate is used for the collection of metal ions from water as well as a way of their *** possible materials design made layered alkali titanates promising for energy(including catalysis,photocatalysts,and battery)and environmental(metal ion concentration from aqueous environments)applications.

关键词： layered alkali titanates photocatalysis hydrogen evdution metal ions collection

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Fecal microbiota transplantation alleviates experimental colitis through the Toll-like receptor 4 signaling pathway

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World Journal of Gastroenterology 2023年第30期29卷 4657-4670页

作者： Xin Wen Rui Xie Hong-Gang Wang Min-na Zhang Le He Meng-Hui Zhang Xiao-Zhong Yang Department of Gastroenterology The Affiliated Huaian No.1 People’s Hospital of Nanjing Medical UniversityHuai’an 223300Jiangsu ProvinceChina

BACKGROUND Fecal microbiota transplantation(FMT)has shown promising therapeutic effects on mice with experimental colitis and patients with ulcerative colitis(UC).FMT modulates the Toll-like receptor 4(TLR4)signaling pathway to treat some other ***,it remains unknown whether this modulation is also involved in the treatment of *** To clarify the necessity of TLR4 signaling pathway in FMT on dextran sodium sulphate(DSS)-induced mice and explain the mechanism of FMT on UC,through association analysis of gut microbiota with colon transcriptome in *** A mouse colitis model was constructed with wild-type(WT)and TLR4-knockout(ko)*** microbiota was transplanted by *** inflammation severity was measured by disease activity index(DAI)scoring and hematoxylin and eosin *** microbiota structure was analyzed through 16S ribosomal Rna *** expression in the mouse colon was obtained by transcriptome *** The ko(DSS+Water)and ko(DSS+FMT)groups displayed indistinguishable body weight loss,colon length,DAI score,and histology score,which showed that FMT could not inhibit the disease in ko *** mice treated with FMT,the relative abundance of Akkermansia decreased,and Lactobacillus became *** particular,compared with those in WT mice,the scores of DAI and colon histology were clearly decreased in the ko-DSS *** structure showed a significant difference between ko and WT *** were the dominant genus in healthy ko *** ineffectiveness of FMT in ko mice was related to the decreased abundance of *** Ontology enrichment analysis showed that differentially expressed genes between each group were mainly involved in cytoplasmic translation and cellular response to Dna damage *** top nine genes correlating with Akkermansia included Aqp4,Clca4a,Dpm3,Fau,Mcrip1,Meis3,Nupr1 L,Pank3,and Rps13(|R|>0.9,P<0.01).CONCLUSION FMT may ameliorate DSS-induced colitis by reg

关键词： Toll-like receptor 4 Fecal microbiota transplantation Colitis Akkermansia Lactobacillus Aquaporin 4 Transcriptome sequencing

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Occurrence of anionic redox with absence of full oxidation to Ru^(5+) in high-energy P2-type layered oxide cathode

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Journal of Energy Chemistry 2023年第9期84卷 153-161页

作者： Jinho Ahn Hyunyoung Park Wonseok ko Yongseok Lee Jungmin Kang Seokjin Lee Sangyeop Lee Eunji Sim Kyuwook Ihm Jihyun Hong Jung-Keun Yoo Kyojin Ku Jongsoon Kim Department of Energy Science Sungkyunkwan UniversitySuwon 16419Republic of Korea SKKU Institute of Energy Science and Technology(SIEST) Sungkyunkwan UniversitySuwon 16419Republic of Korea nano&Interface Research Team Pohang Accelerator LaboratoryPohang 37673Republic of Korea Department of Smart Fabrication Technology Sungkyunkwan UniversitySuwon 16419Republic of Korea Energy Materials Research Center Korea Institute of Science and Technology(KIST)14 Gil 5 Hwarang-roSeongbuk-guSeoul 02792Republic of Korea Carbon Composites Department Composites Research DivisionKorea Institute of Materials Science(KIMS)797 ChangwondaeroChangwon 51508Republic of Korea Department of Materials Science and Engineering Hanbat National UniversityDaejeon 34158Republic of Korea

The anionic redox has been widely studied in layered-oxide-cathodes in attempts to achieve highenergy-density for na-ion batteries(NIBs).It is known that an oxidation state of Mn^(4+) or Ru^(5+) is essential for the anionic reaction of O^(2-)/O~-to occur during na^(+) de/***,here,we report that the anionic redox can occur in Ru-based layered-oxide-cathodes before full oxidation of Ru^(4+)/Ru^(5+).Combining studies using first-principles calculation and experimental techniques reveals that further na^(+) deintercalation from P2-na_(0.33)[Mg_(0.33)Ru_(0.67)]O_(2) is based on anionic oxidation after 0.33 mol na^(+) deintercalation from P2-na_(0.67)[Mg_(0.33)Ru_(0.67)]O_(2) with cationic oxidation of Ru^(4+)/Ru^(4.5+).Especially,it is revealed that the only oxygen neighboring 2Mg/1 Ru can participate in the anionic redox during na^(+) de/intercalation,which implies that the na-O-Mg arrangement in the P2-na_(0.33)[M9_(0.33)Ru_(0.67)]O_(2) structure can dramatically lower the thermodynamic stability of the anionic redox than that of cationic *** the O anionic and Ru cationic reaction,P2-na_(0.67)[Mg_(0.33)Ru_(0.67)]O_(2) exhibits not only a large specific capacity of~172 mA h g^(-1) but also excellent power-capability via facile na^(+) diffusion and reversible structural change during charge/*** findings suggest a novel strategy that can increase the activity of anionic redox by modulating the local environment around oxygen to develop high-energy-density cathode materials for NIBs.

关键词： na-ion batteries P2-type cathode Anionic redox Local environment First-principles calculation

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The apoptotic effect of ozone therapy on mitochondrial activity of highly metastatic breast cancer cell line MDA-MB-231 using in vitro approaches

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Journal of Interventional Medicine 2022年第2期5卷 64-71页

作者： Merve Yildirim Selen Erkisi Hazal Yilmaz naz Unsal Elif inac Yldray Tanrnver Polen kocak Departnent of Genetics and Bioenginering Facaly of Engineering and Architecture Yeditepe University26 Agustos CampusKaqrisdagt CadKaytsdag34755IstanbulTurkey Infirity Regnerative Clini Thy Sites YoluUlus34347IstanbulTurkey Department of Biomedical Engineering Faculy of Enginering Itirve Universit Vadi CampusAzerbaycan CadSariyer34396IstanbulTurkey

Ozone(O) gas is the triatomic state of oxygen and it is used as a disinfection agent due to its strong oxidizing effect, since its discovery in the mid-nineteenth century. Ozone therapy is also an alternative therapeutic approach for some diseases like circulatory disorders, AIDS, asthma, cardiovascular diseases, and certain types of cancer by increasing the oxygen levels in the blood by external addition of ozone to the body. In this study, the therapeutic potential of ozone therapy was examined by inhibiting the growth of breast cancer cells in a dose-dependent procedure. Ozone concentrations varying from 5 to 20 μg/ml were applied to the MDA-MB-231, human breast adenocarcinoma and HUVEC, human umbilical vein endothelium, cell lines, and MDA cells demonstrated an increased rate of death while its migration potential decreases. RT-PCR analysis showed mRna expression levels of pro-apoptotic genes demonstrated higher folds in MDA cells after 10 μg/ml treatment. In the same context,Annexin V/PI and cell cycle analysis also concluded that ozone therapy causes apoptotic cell death on breast tumor cells. The use of ozone therapy for cancer treatment requires further and extensive research. However, this research has shown that ozone therapy is a promising source for cancer treatment in a way by inhibiting the proliferation of breast tumor cells.

关键词： Ozone therapy Antioxidants Apoptosis Breast cancer

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