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乌兰布和沙漠晚第四纪以来环境演化研究进展

中国沙漠 2022年第1期42卷 175-183页

作者：王娜春喜内蒙古师范大学内蒙古自治区湿地环境修复工程实验室内蒙古呼和浩特010022 内蒙古师范大学蒙古高原气候变化与区域响应高校重点实验室内蒙古呼和浩特010022 内蒙古师范大学地理科学学院内蒙古呼和浩特010022

乌兰布和沙漠晚第四纪以来环境演化呈现湖泊与沙漠多次反复的更替模式,湖相层与松散风成砂层迭覆出现。湖泊发育时间段为120—90 ka、60—50 ka、40—20 ka和8—5.5 ka;沙漠扩展时间段为150—130 ka、90—60 ka、50—40 ka、20—10 ka和... 详细信息

乌兰布和沙漠晚第四纪以来环境演化呈现湖泊与沙漠多次反复的更替模式,湖相层与松散风成砂层迭覆出现。湖泊发育时间段为120—90 ka、60—50 ka、40—20 ka和8—5.5 ka;沙漠扩展时间段为150—130 ka、90—60 ka、50—40 ka、20—10 ka和5.5 ka—近现代时期。由于时空尺度差异,构造活动、气候变化和黄河变迁是更新世以来乌兰布和沙漠环境演化的主要原因,直到全新世晚期人类活动加剧了沙漠的进一步扩张。乌兰布和沙漠环境变化剧烈,成为中国北方地区的关键地带。未来研究需准确提炼科学问题,丰富时空尺度和测年方法,将湖岸阶地剖面与岩芯相结合,以准确认识复杂的沙漠化过程,这在应对气候变化、生态文明建设和区域可持续发展等方面将发挥关键作用。

关键词：干旱区环境演化时空尺度驱动机制

葡萄籽原花青素对氯化钴诱导的视网膜神经节细胞RGC-5缺氧损伤的保护作用

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浙江大学学报（医学版） 2015年第1期44卷 24-29页

作者：林卡娜林美丽魏尔清浙江大学医学院附属第二医院药剂科浙江杭州310009 浙江大学医学院药理学系浙江杭州310058

目的：探讨体外葡萄籽原花青素对氯化钴致视网膜神经节细胞RGC－5缺氧损伤模型的保护作用。方法：采用氯化钴（400μmol／L）诱导RGC－5细胞化学缺氧损伤，观察葡萄籽原花青素（40μg／mL ）对缺氧的抗损伤作用。以MTT法检测细胞存活率... 详细信息

目的：探讨体外葡萄籽原花青素对氯化钴致视网膜神经节细胞RGC－5缺氧损伤模型的保护作用。方法：采用氯化钴（400μmol／L）诱导RGC－5细胞化学缺氧损伤，观察葡萄籽原花青素（40μg／mL ）对缺氧的抗损伤作用。以MTT法检测细胞存活率；Hoechst 33342荧光染色法观察细胞凋亡情况；以H2 DCFDA荧光法检测细胞内活性氧的变化；实时定量PCR检测细胞Bcl－2、半胱酰胺天冬氨酸蛋白酶（ caspase ）9和caspase 3 mRna水平。结果：与缺氧对照组比较，葡萄籽原花青素预处理可提高细胞存活率（ P＜0．001）、减少细胞凋亡（ P＜0．001），并减少细胞内的活性氧（ P ＜0．001）；葡萄籽原花青素还显著升高 Bcl－2基因的表达（ P ＜0．001），显著降低caspase 9和caspase 3基因的表达（ P＜0．001）。结论：葡萄籽原花青素对氯化钴诱导的视网膜神经节RGC－5细胞缺氧损伤起保护作用，其保护作用可能与细胞内活性氧减少、Bcl－2基因表达上调及casepase 9和casepase 3基因表达下调有关。

关键词：葡萄/化学原花青素类/药理学钴/药理学视网膜神经节细胞/药物作用缺氧/治疗

猪流行性腹泻病毒S2基因B细胞表位嵌入型S-层蛋白在副干酪乳杆菌中的表达与鉴定

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中国畜牧兽医 2022年第10期49卷 4004-4011页

作者：白伟琴卡楚拉乌志勇苗苗塔娜格日勒图内蒙古农业大学兽医学院呼和浩特010010

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)乳酸菌活菌疫苗载体。【方法】应用Dna重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEDV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达... 详细信息

【目的】构建新型猪流行性腹泻病毒(Porcine epidemic diarrhea virus,PEDV)乳酸菌活菌疫苗载体。【方法】应用Dna重组技术将嗜酸乳杆菌S-层蛋白(SLP)基因及PEDV S2基因B细胞表位(EpitopeS2)融合基因(SLP-EpitopeS2)克隆到乳酸杆菌表达载体pTRK892中,构建重组载体pTRK-SLP-EpitopeS2,通过电转化方法将重组质粒导入副干酪乳杆菌中,获得重组副干酪乳杆菌。分别用SDS-PAGE、Western blotting和免疫荧光试验(IFA)鉴定目的蛋白在副干酪乳杆菌中的表达。【结果】PCR结果显示,成功扩增出大小为1400 bp的目的条带,与插入融合基因大小一致,双酶切结果出现大小分别为1400和4700 bp的2条带,基因测序结果显示无碱基缺失和突变等,从而确定重组质粒pTRK-SLP-EpitopeS2构建正确。SDS-PAGE、Western blotting结果显示,在48 ku处出现与理论值大小一致的目的蛋白条带,表明融合基因SLP-EpitopeS2在副干酪乳杆菌中得到有效表达。IFA结果显示,与对照组副干酪乳杆菌相比,重组副干酪乳杆菌均能被激发出特异性绿色荧光信号,与高浓度氯化锂(LiCl)洗脱下来的菌体膜蛋白样品补充鉴定试验结果相吻合。表明融合蛋白SLP-EpitopeS2可能在副干酪乳杆菌的菌体表面表达。【结论】成功构建了PEDV EpitopeS2及嗜酸乳杆菌SLP嵌入型融合表达载体pTRK-SLP-EpitopeS2,为乳杆菌活菌载体疫苗相关研究奠定了基础。

关键词：猪流行性腹泻病毒(PEDV) S2基因副干酪乳杆菌表达鉴定

Surgery for Early Stage Invasive Cervical Cancer. Experience of the Oncology Department of Ledantec Hospital

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Journal of Cancer Therapy 2022年第2期13卷 80-87页

作者： Sidy ka Yaméogo Bonaventure Mamadou M. Dieng Souleymane Dieng Jaafar Thiam Salif Baldé Ahmadou Dem Centre Hospitalier Universitaire Le Dantec Dakar Sénégal

Background: Surgery is the treatment for early-stage cervical cancer. Radiochemotherapy is used in the treatment of locally advanced stages. But the choice of treatment can be difficult. Objective: The objective of this work was to evaluate the therapeutic aspects of cervical cancer in the early stages ia to Iia in the oncology department of A Ledantec Hospital in Dakar. Patients and methods: This was a descriptive retrospective study, from January 2015 to December 2020, in the oncology department of A Ledantec Hospital, including all the patients who had been treated for early-stage invasive cervical cancer. Results: We included 28 patients. The average age was 49.54 years old. There were 11 patients (39.3%) with a stage ≤ IB1. Surgery was the first in 14 patients (50%). The initial approach was a midline supra and subumbilical laparotomy. The surgery was R0 in 65.22%. Postoperative complications were found in 4 patients including 1 case of operative wound suppuration, 1 case of dysuria, 1 case of postoperative eventration and 1 case of rectovaginal fistula. Concomitant Radiochemotherapy was neoadjuvant in 7 cases (25%), adjuvant in 14 cases (50%). The chemotherapy was neoadjuvant in 09 cases (56.25%) and adjuvant in 7 cases (43.75%) with minor toxicities. We had recorded 2 deaths. Conclusion: The treatment of the early stages of invasive cervical cancer is based on surgery. Neoadjuvant treatments may be useful in particular situations.

关键词： Cancer Cervix Early Treatment

Comparative analysis of robotic vs laparoscopic radical hysterectomy for cervical cancer

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World Journal of Clinical Cases 2019年第20期7卷 3185-3193页

作者： Li Chen Li-Ping Liu na Wen Xiao Qiao Yuan-Guang Meng Department of Obstetrics and Gynecology Chinese PLA General HospitalBeijing 100853China Department of Obstetrics and Gynecology The First Central Hospital of BaodingBaoding 071000Hebei ProvinceChina Medical Big Data Center Chinese PLA General HospitalBeijing 100853China

BACKGROUND Cervical cancer is the most common gynecological malignancy,ranking first in female reproductive malignancies with more than 500000 new cases and 275000 deaths each ***,open radical hysterectomy is considered the standard surgical procedure for the treatment of resectable cervical *** latest guidelines from the national Comprehensive Cancer Network and the European Society of Gynecological Oncology suggest that open surgery and laparoscopic surgery(using traditional laparoscopic or robotic techniques)are the main surgical approaches for radical hysterectomy for patients with stage ia2-Iia cervical *** surgery has been increasingly used in abdominal surgery and has shown more beneficial *** To analyse the perioperative conditions,complications,and short-term and longterm effects in patients undergoing robotic radical hysterectomy(RRH)and laparoscopic radical hysterectomy(LRH)to compare their clinical efficacy,safety,and *** The perioperative data of patients undergoing RRH and LRH were extracted and collected from the database of surgical treatments for cervical cancer for statistical *** Of the patients,342 underwent LRH for cervical cancer,and 216 underwent *** total complication rate was 9.65%(20 patients)in the RRH group and 17.59%(60 patients)in the LRH *** complication rate was significantly lower in the RRH group than in the LRH *** was no significant difference in the follow-up period(P=0.658).The total recurrence rates were 15.7%and 12%in the RRH and LRH groups,*** progression-free survival time was 28.91±15.68 mo and 28.34±15.13 mo in the RRH and LRH groups,respectively(P=0.669).The overall survival(OS)rates were 92.13%and 94.45%in the RRH and LRH groups,respectively(P=0.292).The OS time was 29.87±15.92 mo and 29.41±15.14 mo in the RRH and LRH groups,respectively(P=0.732).The survival curves and the progression-free survival curves were not statis

关键词： Cervical cancer Robotic radical hysterectomy Laparoscopy Survival

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

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Signal Transduction and Targeted Therapy 2021年第10期6卷 3108-3118页

作者： Xiang-na Zhao Yue You Xiao-Ming Cui Hui-Xia Gao Guo-Lin Wang Sheng-Bo Zhang Lin Yao Li-Jun Duan ka-Li Zhu Yu-Ling Wang Li Li Jian-Hua Lu Hai-Bin Wang Jing-Fang Fan Huan-Wei Zheng Er-Hei Dai Lu-Yi Tian Mai-Juan Ma Center for Disease Control and Prevention of Chinese People's Liberation Army BeijingChina The Walter and Eliza Hall Institute of Medical Research ParkvilleVicAustralia Department of Medical Biology The University of MelbourneParkvilleVicAustralia State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijingChina The Fifth Hospital of Shijiazhuang Hebei Medical UniversityShijiazhuangChina

While some individuals infected by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)present mild-to-severe disease,many SARS-CoV-2-infected individuals are *** sought to identify the distinction of immune response between asymptomatic and moderate *** performed single-cell transcriptome and T-cell/B-cell receptor(TCR/BCR)sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic,moderate,and severe patients with healthy *** patients displayed increased CD56^(bri)CD16^(-) natural killer(NK)cells and upregulation of interferon-gamma in effector CD4^(+) and CD8^(+) T cells and NK *** showed more robust TCR clonal expansion,especially in effector CD4^(+) T cells,but lack strong BCR clonal expansion compared to moderate ***,asymptomatic patients have lower interferon-stimulated genes(ISGs)expression in general but large interpatient variability,whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe *** data provide evidenee of different immune signatures to SARS-CoV-2 in asymptomatic infections.

关键词： patients asymptomatic interferon

新型含二氢喹唑啉酮的咖啡因衍生物的合成及生物活性研究

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有机化学 2023年第1期43卷 299-307页

作者：汪蕾于淑晶杨娜王宝雷南开大学化学学院元素有机化学国家重点实验室天津300071

基于在咖啡因8-位引入二氢喹唑啉酮活性基团的策略,以茶碱和靛红酸酐/5-溴靛红酸酐为原料,通过N-甲基化、钯催化偶联、亲核加成-消除及缩合等多步反应,合成了13个未见文献报道的新型含二氢喹唑啉酮结构的咖啡因衍生物.生物活性测试结果... 详细信息

基于在咖啡因8-位引入二氢喹唑啉酮活性基团的策略,以茶碱和靛红酸酐/5-溴靛红酸酐为原料,通过N-甲基化、钯催化偶联、亲核加成-消除及缩合等多步反应,合成了13个未见文献报道的新型含二氢喹唑啉酮结构的咖啡因衍生物.生物活性测试结果表明,大部分化合物对小菜蛾(Plutella xylostella L.)表现出显著的杀虫活性,其中1,3,7-三甲基-8-(4-(4-氧亚基-3-丙基-1,2,3,4-四氢喹唑啉-2-基)苯基)-3,7-二氢-1H-嘌呤-2,6-二酮(ia)和8-(4-(6-溴-3-(4-氟苄基)-4-氧亚基-1,2,3,4-四氢喹唑啉-2-基)苯基)-1,3,7-三甲基-3,7-二氢-1H-嘌呤-2,6-二酮(Im)在50 mg·L^(-1)时对小菜蛾的致死率分别可达50%和43%,尤其是ia在10 mg·L^(-1)时仍有20%的致死率.部分化合物也表现出了良好的除草活性,特别是ia在100 mg·L^(-1)浓度下对油菜胚根的生长抑制率达到96.6%,优于对照药氯磺隆.此外,部分化合物在50 mg·L^(-1)时对油菜菌核病菌(Sclerotinia sclerotiorum)、辣椒疫霉病菌(Phytophthora capsicum)和苹果轮纹病菌(Physalospora piricola)等具有50%以上的抑制活性,其中ia对辣椒疫霉病菌的抑制率可达80.6%,远超对照物咖啡因.ia在杀虫、除草和抑菌测试中大都表现出良好的活性,可作为新型农药先导化合物继续开展深入结构优化.本研究结果对基于天然产物结构的新型农药分子设计具有一定的参考价值.

关键词：黄嘌呤咖啡因二氢喹唑啉酮合成杀虫活性抑菌活性除草活性

Geochemistry of Sediments from the Lopare Basin(Bosnia and Herzegovina):Implications for Paleoclimate,Paleosalinity,Paleoredox and Provenance

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Acta Geologica Sinica(English Edition) 2020年第5期94卷 1591-1618页

作者： AleksandraŠAJNOVIĆ Nenad GRBA Franz NEUBAUER Milica kaŠANIN-GRUBIN Ksenija STOJANOVIĆ Nenad PETKOVIĆ Branimir JOVANČIĆEVIĆ University of Belgrade Center of ChemistryICTMStudentski trg 12-1611000 BelgradeSerbia University of Novi Sad Faculty of SciencesDepartment of ChemistryBiochemistry and Environmental ProtectionTrg Dositeja Obradovica 321000 Novi SadSerbia Paris Lodron University of Salzburg Department of Geography and GeologyHellbrunnerstrasse 345020 SalzburgAustria University of Belgrade Faculty of ChemistryStudentski trg 12-1611000 BelgradeSerbia Rio Tinto–Rio Sava Exploration Takovska 4511000 BelgradeSerbia

A combined inorganic and organic geochemical study was carried out on marls and mudstones collected from the Lower Miocene Lopare Basin,Bosnia and Herzegovina.A total of 46 samples collected from two boreholes,Pot 1(depth of 193 m)and Pot 3(depth of 344 m),showed that element abundances like boron(B),lithium(Li),strontium(Sr),uranium(U),chromium(Cr),nickel(Ni),magnesium(Mg),sodium(na)and calcium(Ca)are much higher than average than in the upper continental crust(UCC).Chemical composition indicates at least two sources:(i)Mesozoic ophiolites occurring in the north of the investigated area,and(ii)dacito-andesitic pyroclastics(Mesozoic to Cenozoic).Lopare Basin sedimentation was influenced by strong evaporation resulting in a partly hypersaline lake,which formed during a warm climatic period,probably during the Miocene Climatic Optimum.A brief episode of humid climate conditions resulted in the basin fillingup and deposition of felsic sediments enriched in thorium(Th).Organic geochemistry shows that the majority of studied sediments contains predominantly immature to marginally mature algal organic matter(OM).The biomarker patterns are generally in agreement with the geological history of the Lopare Basin and inorganic and mineralogical ***,the molecular distribution of n-alkanes as reliable climatic andδ-MTTC as paleosalinity indicators do not support this conclusion.

关键词： geochemistry provenance analysis paleoenvironmental conditions salinity/hypersalinity biomarker Lopare Basin Bosnia and Herzegovina

Viral kinetics of Enterovirus 71 in human habdomyosarcoma cells

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World Journal of Gastroenterology 2011年第36期17卷 4135-4142页

作者： Jing Lu Li-na Yi Hsiang-Fu Kung Ming-Liang He Ya-Qing He Hong Zan Stanley Ho Center for Emerging Infectious Diseases and Li Ka Shing Institute of Health SciencesFaculty of MedicineThe Chinese University of Hong KongHong KongChina Shenzhen Center for the Disease Control and Prevention Shenzhen 518055Guangdong ProvinceChina Institute for Immunology University of California at IrvineCA 92697United States

AIM:To characterise the viral kinetics of enterovirus 71 (EV71).METHODS:In this study,human rhabdomyosarcoma (RD) cells were infected with EV71 at different multiplicity of infection (MOI).After infection,the cytopathic effect (CPE) was monitored and recorded using a phase contrast microscope associated with a CCD camera at different time points post viral infection (0,6,12,24 h post infection).Cell growth and viability were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in both EV71 infected and mock infected cells at each time ***71 replication kinet-ics in RD cells was determined by measuring the total intracellular viral Rna with real-time reverse-transcription polymerase chain reaction (qRT-PCR).Also,the intracellular and extracellular virion Rna was isolated and quantified at different time points to analyze the viral package and *** expression of viral protein was determined by analyze the levels of viral structure protein VP1 with Western ***:EV71 infection induced a significant CPE as early as 6 h post infection (p.i.) in both RD cells infected with high ratio of virus (MOI 10) and low ratio of virus (MOI 1).In EV71 infected cells,the cell growth was inhibited and the number of viable cells was rapidly decreased in the later phase of ***71 virions were uncoated immediately after *** intracellular viral Rna began to increase at as early as 3 h *** the exponential increase was found between 3 h to 6 h *** both infected *** viral structure protein synthesis,results from western-blot showed that intracellular viral protein VP1 could not be detected until 6 h *** the cells infected at either MOI 1 or MOI 10;and reached the peak at 9 h *** the cells infected with EV71 at both MOI 1 and MOI ***,the viral package and secretion were also actively processed as the virus underwent rapid *** viral package kinetics was comparable for both MOI 1

关键词：细胞病变效应肠病毒动力学人类逆转录聚合酶链反应病毒结构蛋白免疫印迹分析病毒感染