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Cloning and Bioinformatics Analysis of hcp Gene in Aeromonas hydrophila

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Asian Agricultural Research 2024年第3期16卷 36-40页

作者： Fan LI Xingjun FU Haiyun FENG shi WANG Weijie ZHANG Huanying PANG na WANG Fisheries College Guangdong Ocean UniversityZhanjiang 524025China Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture&Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes Zhanjiang 524025China Chinese Academy of Inspection and Quarantine Beijing 100176China

[Objectives]To explore the function of hcp gene in Aeromonas hydrophila.[Methods]A pair of specific primers was designed referring to the hcp gene sequence of *** hcp gene was amplified by PCR,and performed bioinformatics analysis.[Results]The hcp gene had a total length of 1650 bp and encoded 549 amino *** theoretical molecular weight of the protein predicted was about 59476.44 *** predicting the N-terminal signal peptide structure of the amino acid sequence,neither obvious signal peptide cleavage site nor signal peptide was found,and the protein had no transmembrane *** amino acid sequence had a N-glycosylation site,4 protein kinase C phosphorylation sites,7 casein kinase II phosphorylation sites,9 N-myristoylation sites,4 isoprene binding sites,10 microbody C-terminal target signal sites,and an ATP/GTP binding site motif A(P-ring).The amino acid sequence of hcp gene of *** was performed homology analysis with other Aeromonas strains,and it showed higher homology with *** the secondary structure,theα-helix,β-sheet,random coil and extended strand accounted for 45.36%,6.01%,37.52%and 11.11%,*** tertiary structure model consisted of 18α-helix and 22β-*** of protein-protein network interaction demonstrated that the proteins interacting with Hcp protein were AHA_3407,nrfA,nirB-1,nirB-2 and AHA_1112.[Conclusions]Through the bioinformatics prediction results,the basic information of hcp gene of *** is preliminarily understood,and the possible function of this protein is predicted,in order to provide guidance for subsequent vaccine research.

关键词： Aeromonas hydrophila Gene amplification hcp gene Bioinformatics analysis

Reflective ultrathin light-sheet microscopy with isotropic 3D resolutions

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Photonics Research 2024年第2期12卷 271-281页

作者： YUE WANG DASHAN DONG WENKAI YANG RENXI HE MING LEI KEBIN shi State Key Laboratory for Mesoscopic Physics Department of PhysicsPeking UniversityBeijing 100871China State Key Laboratory of Membrane Biology School of Life Sciencesand Biomedical Pioneering Innovation Center(BIOPIC)Peking UniversityBeijing 100871China School of Physics Xi’an Jiaotong UniversityXi’an 710049China Collaborative Innovation Center of Extreme Optics Shanxi UniversityTaiyuan 030006China Peking University Yangtze Delta Institute of Optoelectronics Nantong 226010China

Light-sheet fluorescence microscopy(LSFM)has played an important role in bio-imaging due to its advantages of high photon efficiency,fast speed,and long-term imaging *** perpendicular layout between LSFM excitation and detection often limits the 3D resolutions as well as their ***,we report on a reflective type light-sheet microscope with a mini-prism used as an optical path *** conventional high na objectives can be used both in excitation and detection with this *** resolutions in 3D down to300 nm could be achieved without *** proposed method also enables easy transform of a conventional fluorescence microscope to high performance light-sheet microscopy.

关键词： resolution excitation light

Effects of interleukin-10 on activation and apoptosis of hepatic stellate cells in fibrotic rat liver

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World Journal of Gastroenterology 2006年第12期12卷 1918-1923页

作者： Li-Juan Zhana Wei-Da Zheng Mei-na shi Department of Gastroenterology Union Hospital of Fujian Medical University.Fuzhou 350001.Fujian Province.China

AIM:To study the effects of interleukin-10(IL-10)on the expression of α-smooth muscle actin(α-SMA),nuclear factor-κB(NF-κB)and Fas/Fas ligand(FasL)inhepatic stellate cells of experimental rats with ***:Sixty clean SD rats were randomly dividedinto control group(group N),liver fibrotic group(groupC)and IL-10 treatment group(group I).Control groupreceived intraperitoneal injection of saline(2ml·kg^(-1)),twicea *** group was injected intraperitoneallywith 50% carbon tetrachloride(CCl_4)(2 ml·kg^(-1)),twicea ***-10 treatment group was given IL-10 at adose of 4 μg·kg^(-1)20 minutes before CCl_4 administrationfrom the third *** stellate cells(HSCs)wereisolated from these rats at the seventh and eleventhweeks during the course of liver fibrosis,*** expression of α-SMA and NF-κB in HSCs wasmeasured by S-P *** expressionof Fas and FasL mRna was measured by ***,liver tissues were harvested from threegroups at the same ***:The CCl_4- induced experimental rat hepaticfibrosis model was established *** purityof extracted hepatic stellate cells was about 95% andthe yield of hepatic stellate cells was 1.2-2.3×10~6/g livertissue *** positive expression of α-SMA andNF-κB was 36.5% and 28.5% respectively in group *** positive levels of α-SMA and NF-κB were increasedsignificantly in group C compared to group N(Pnals decreased significantly(P0.05).The mRna of Fasand FasL in group C was significantly increased in a time-dependent manner compared to that in control *** treated with IL-10,the expression level of Fas andFasL was higher in group I than in group ***:The positive expressio

关键词：白细胞介素-10 细胞调亡肝脏星形细胞肝纤维化

An Analytic Model of Subminiature Auditory Sensation System for Sound Source Localization

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Chinese Journal of Biomedical Engineering(English Edition) 2007年第4期16卷 148-154页

作者： WANG Qing-sheng TA na RAO Zhu-shi State Key Laboratory of Mechanical System and Vibration Shanghai Jiao Tong University Shanghai 200240 China

It is reported that some types of insects have a remarkable ability to detect the direction of an incident sound even though its acoustic sensory organs are in very close proximity each other. Maybe the ears are jointed by a cuticular structure with which the separated motions can be coupled mechanically and thus be magnified. In this paper, a detailed model is setup to describe the principle of this type of localization using a mechanical coupled structure. The transfer functions and the responses of the model in terms of time and frequency are analyzed to describe the mechanism of its ability of directional hearing. This analytical model provides a method to design the experimental model for the predetermined incident sound pressure, and the analysis of this model shows that this structure have the ability to determine the direction of the incident stimulus.

关键词：解析模型微型听觉感觉系统拟寄生昆虫声音源

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当代我国社会经济价值观嬗变的报告

河南大学学报（社会科学版） 2010年第3期50卷 81-85页

作者：史娜辽宁大学马克思主义学院辽宁沈阳110036

我国社会主义市场经济的确立和发展,打破了原有的计划经济体制,为商品交易和经济发展注入了活力,我国社会的经济价值观领域也开始进入从传统向现代的全面转型时期。市场经济内在的诚信观、契约观和竞争意识等价值观的觉醒和发扬,使我国... 详细信息

我国社会主义市场经济的确立和发展,打破了原有的计划经济体制,为商品交易和经济发展注入了活力,我国社会的经济价值观领域也开始进入从传统向现代的全面转型时期。市场经济内在的诚信观、契约观和竞争意识等价值观的觉醒和发扬,使我国传统的经济价值观受到巨大冲击,同时也带来了资本主义经济价值观的外来影响。根据《中国公民价值观蓝皮书》的调查报告,可以看出我国公民的市场经济价值观转变基本成功,但是它与未来的现代经济价值观仍有一定差距。

关键词：市场经济经济价值观嬗变

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改革开放以来我国社会价值观的变革与建构

河南大学学报（社会科学版） 2010年第2期50卷 51-55页

作者：史娜辽宁大学马克思主义学院辽宁沈阳110036

中国自改革开放30年以来,社会主义市场经济迅猛发展,科学技术水平日益提高,物质财富极大丰富,老百姓的生活发生了翻天覆地的变化,社会的生产方式、经济政治制度和人们的价值观念亦发生了巨大变化,社会价值观呈现出多元化和多样性的特点... 详细信息

中国自改革开放30年以来,社会主义市场经济迅猛发展,科学技术水平日益提高,物质财富极大丰富,老百姓的生活发生了翻天覆地的变化,社会的生产方式、经济政治制度和人们的价值观念亦发生了巨大变化,社会价值观呈现出多元化和多样性的特点。在此背景下,准确地把握改革开放以来我国社会价值观变革的本质特征及其内在根源,确立科学合理的主导价值观并使之发挥积极的引导作用,对冲突的价值观进行合理整合,建构符合社会发展方向的价值观体系,这些是应对当今社会价值观变革的重要手段。

关键词：改革开放社会价值观变革社会主义核心价值体系整合建构

高校大型精密仪器设备使用现状及高效管理模式探讨

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实验室研究与探索 2022年第11期41卷 308-312页

作者：楚华琴时娜于宝证合肥工业大学国有资产管理处合肥230009 合肥工业大学财务处合肥230009 合肥工业大学教学与研究评估处合肥230009

高等学校大型精密仪器设备的充分利用和仪器使用平台的建设是高校实验教学、科学研究、人才培养、技术创新、社会服务的重要基础。为了提高高校大型精密仪器设备管理及使用效率,对合肥工业大学大型精密仪器设备使用效益进行分析,针对设... 详细信息

高等学校大型精密仪器设备的充分利用和仪器使用平台的建设是高校实验教学、科学研究、人才培养、技术创新、社会服务的重要基础。为了提高高校大型精密仪器设备管理及使用效率,对合肥工业大学大型精密仪器设备使用效益进行分析,针对设备管理工作中存在的主要问题提出在购置论证、共享使用、实验室队伍和维修维护4个方面的相应对策,为其他高等院校在促进大型精密仪器的共享和管理工作上提供借鉴。

关键词：精密仪器现状与问题管理模式高等学校

学校铸牢中华民族共同体意识教育评价指标体系构建——基于“意识三态观”的分析框架

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民族教育研究 2023年第3期34卷 20-28页

作者：施娜王晓宇吴明海中央民族大学教育学院北京100081 中央民族大学预科教育学院北京100081 威海市文登区人才创新发展中心山东威海264209

建立健全基于诊断和干预功用的学校铸牢中华民族共同体意识教育评价指标体系,能够在很大程度上“反哺”学校铸牢中华民族共同体意识教育的实践效能提升。作为具有整体性视野的本土特色理论,“意识三态观”对于构建学校铸牢中华民族共同... 详细信息

建立健全基于诊断和干预功用的学校铸牢中华民族共同体意识教育评价指标体系,能够在很大程度上“反哺”学校铸牢中华民族共同体意识教育的实践效能提升。作为具有整体性视野的本土特色理论,“意识三态观”对于构建学校铸牢中华民族共同体意识教育评价指标体系具有良好的范式指导意义。基于“意识三态观”的学校铸牢中华民族共同体意识教育评价指标体系可囊括宏观(国家主流意识形态)、中观(学校意识生态)、微观(个体意识心态)共3个一级指标,其中宏观层面涉及“政策引领”“组织领导”“条件保障”和“社会支持”共4个二级指标;中观层面包括“工作统筹”“过程保障”“教育实施”和“实施反馈”共4个二级指标;微观层面关涉“教师发展”和“学生发展”共2个二级指标。最终建构出由3个一级指标、10个二级指标和37个三级指标勾连互嵌的学校铸牢中华民族共同体意识教育评价指标体系,以推进学校在铸牢中华民族共同体意识教育工作中的主体协同化、内容精准化、方法多元化和成效显性化。

关键词：学校铸牢中华民族共同体意识教育教育评价 “意识三态观”

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潍坊生姜茎基腐病拮抗菌株筛选及鉴定

潍坊工程职业学院学报 2022年第5期35卷 84-89页

作者：石娜娜袁成磊潍坊工程职业学院山东潍坊262500

为有效防治生姜茎基腐病的发生,促进生姜种植绿色可持续发展,以潍坊生姜茎基腐病的主要致病菌群结腐霉为病原菌,筛选出可以拮抗大姜茎基腐病致病菌的菌株,经鉴定具有拮抗作用的菌株分别为龙舌兰芽孢杆菌(Bacillus tequilensis)、枯草芽... 详细信息

为有效防治生姜茎基腐病的发生,促进生姜种植绿色可持续发展,以潍坊生姜茎基腐病的主要致病菌群结腐霉为病原菌,筛选出可以拮抗大姜茎基腐病致病菌的菌株,经鉴定具有拮抗作用的菌株分别为龙舌兰芽孢杆菌(Bacillus tequilensis)、枯草芽孢杆菌(Bacillus subtilis)和黑曲霉(Aspergillus niger)。

关键词：生姜茎基腐病拮抗菌