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Integrative analysis of in vivo recording with single-cell Rna-seq data reveals molecular properties of light-sensitive neurons in mouse V1

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Protein & Cell 2020年第6期11卷 417-432页

作者： Jianwei Liu Mengdi Wang Le Sun na clara pan Changjiang Zhang Junjing Zhang Zhentao Zuo Sheng He Qian Wu Xiaoqun Wang State Key Laboratory of Brain and Cognitive Science CAS Center for Excellence in Brain Science and Intelligence TechnologyInstitute of Brain-Intelligence Technology(Shanghai)Institute of BiophysicsChinese Academy of SciencesBeijing 100101China State Key Laboratory of Cognitive Neuroscience and Learning Beijing Normal UniversityBeijing 100875China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China IDG/McGovem Institute for Brain Research Beijing Normal UniversityBeijing 100875China Advanced Innovation Center for Human Brain Protection Beijing Institute for Brain DisordersCapital Medical UniversityBeijing 100069China

Vision formation is classically based on projections from retinal ganglion cells(RGC)to the lateral geniculate nucleus(LGN)and the primary visual cortex(V1).Neurons in the mouse V1 are tuned to light *** the cellular information of the retina and the LGN has been widely studied,the transcriptome profiles of single light-stimulated neuron in V1 remain *** our study,in vivo calcium imaging and whole-cell electrophysiological patch-clamp recording were utilized to identify 53 individual cells from layer 2/3 of V1 as lightsensitive(LS)or non-light-sensitive(NS)by single-cell light-evoked calcium evaluation and action potential *** contents of each cell after functional tests were aspirated in vivo through a patch-clamp pipette for mRna ***,the three-dimensional(3-D)morphological characterizations of the neurons were reconstructed in a live mouse after the whole-cell *** sequencing results indicated that V1 neurons with a high expression of genes related to transmission regulation,such as Rtn4r and Rgs7,and genes involved in membrane transport,such as na+/K+ATPase and NMDA-type glutamatergic receptors,preferentially responded to light ***,an antagonist that blocks Rtn4r signals could inactivate the neuronal responses to light stimulation in live *** conclusion,our findings of the vivo-seq analysis indicate the key role of the strength of synaptic transmission possesses neurons in V1 of light sensory.

关键词： light sensitivity vivo-seq patch-seq calcium imaging in vivo whole cell recording in vivo

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Galanin receptor 2 mediates galanin-induced activation of large conductance Ca2+-dependent K+ channels(BK) in human embryonic kidney(HEK293) cells

Galanin receptor 2 mediates galanin-induced activation of la...

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中国神经科学学会第十届全国学术会议

作者： Yun-Fei BAI na clara pan Zhi-Qing David XU Beijing Center of Neural Regeneration & Repair Department of NeurobiologyBeijing Key Laboratory of Brain Major DisordersCapital Medical University

Objective The large conductance Ca-activated K(BK) channels,which are widely distributed in the central nervous system(CNS),have been considered as intracellular calcium sensors in neuron and play an important feedback role in controlling Ca flux and subsequent Ca-dependent processes including neurotransmitter release and cellular *** The effects of neuropeptide galanin on BK channels in human embryonic kidney(HEK293) cells co-expressing both GalR2 and BK alpha subunit were examined by determining the whole-cell currents and single-channel ***(1) The application of galanin enhanced the currents of BK channels,in a PTX-independent and concentration-dependent *** ED50 value of dose-dependent curve was 71.8+16.9 nM.(2) Galanin-induced activation of BK was mediated by GalR2,but not GalR1 nor GalR3 as galanin did not facilitate BK current in the cells co-expressed cDnas of BK with GalR1 or GalR3.(3) Chelating intracellular Ca by either the slow Ca buffer EGTA or the fast Ca buffer BAPTA in the pipette solution abolished galanin-mediated activation of BK channels,indicating the important role of Ca in galanin-induced BK current.(4) The galanin-induce BK current persisted when the bath solution was replaced by Ca-free solution,suggesting the extracellular Ca in not required to lead activation of BK channels.(5) The galanin-induced BK channels activation mediated by the Ca efflux from the sarcoplasmic reticulum /endoplasmic reticulum(SR/ER) was confirmed by application of thapsigargin,an irreversible inhibitor that emptied Ca pool from SR/ER.(6) Inositol-1,4,5-triphosphate receptor(IP3R) on ER was identified as the mediator responsible for increased intracellular Ca that activated BK *** Activation of GalR2,by its ligand galanin,led to elevation of intracellular Ca level due to Ca efflux through IP3R from ER and open BK channels.

关键词： BK channel Ca2+ efflux Galanin receptor 2 IP3R

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Fluorescent Probes for Cysteine and Cysteine Oxidation Imaging

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Chemical Research in Chinese Universities 2024年第2期40卷 225-236页

作者： ZHANG Xia LI Guocheng pan Wei LI na LI Yanhua TANG Bo College of Chemistry Chemical Engineering and Materials ScienceKey Laboratory of Molecular and Nano ProbesMinistry of EducationCollaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of ShandongInstitute of Molecular and Nano ScienceShandong Normal UniversityJinan250014P.R.China Laoshan Laboratory Qingdao266237P.R.China

Cysteine is an important regulator of redox *** to the nucleophilic and oxidative sensitivity,cysteine residues in proteins can be oxidized by intracellular reactive oxygen species(ROS),which can lead to protein structural and functional ***,the development of fluorescent probes to image cysteine and cysteine oxidation is of great significance for the study of redox homeostasis in living *** this review,the development of fluorescent probes for imaging cysteine and cysteine oxidation was ***,we further analyzed defects of the reported fluorescent probes and made suggestions for the future development of fluorescent *** expect that this review can not only provide a deeper understanding of the role of cysteine and cysteine oxidation in oxidative stress,but also broaden the application of fluorescent probes in imaging cysteine and cysteine oxidation.

关键词： Cysteine Cysteine oxidation Fluorescent probe Oxidative stress

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Flexible mille-feuille structure electromagnetic interference shielding film with excellent thermal conductivity and Joule heating

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nano Research 2024年第5期17卷 4544-4554页

作者： Dingwen Yin Huijuan Xiu Simin Wang Yanfei pan na Li Rui Cheng Shaoyan Huang Sha Fan Jinbao Li College of Bioresources Chemical&Materials Engineering Shaanxi University of Science&TechnologyXi'an 710021China national Demonstration Center for Experimental Light Chemistry Engineering Education Shaanxi Provincial Key Laboratory of Papermaking Technology and Specialty Paper DevelopmentXi'an 710021China College of Material Science and Art Design Inner Mongolia Agricultural UniversityHohhot 010018China

The intelligent electronic devices have urgent demands for electromagnetic interference(EMI)shielding films with excellent heat dissipation ***,it is challenging to obtain excellent EMI shielding and thermal conductivity performances ***,inspired by mille-feuille structure,the multifunctional EMI shielding films developed by a layer-by-layer self-assembly and hot-pressing *** ingenious introduction of silver nanoparticles(AgNPs)with large specific surface area and highly conductive into the network formed by TEMPO-oxidized cellulose nanofibrils(TOCNFs)with large aspect ratio to form the TOCNFs/*** the graphene nanoplates(GNPs)with high conductivity loss distributed alternately with TOCNFs/AgNPs to construct mille-feuille structure,which had highly efficient conductive network,complete thermally conduction pathway and rich heterogeneous ***,the designed films presented high electrical conductivity of 8520 S/cm,superb EMI effectiveness(SE)of 98.05 dB,and excellent thermal conductivity of 18.82 W/(m·K).Furthermore,the films possessed outstanding Joule heating performances with low voltages,including high heating temperature(100℃),fast response time(nating thermal conductivity and Joule heating performances have substantial application in flexible electronics,electromagnetic waves shielding and thermal management.

关键词： TEMPO-oxidized cellulose nanofibrils/silver nanoparticles(TOCNFs/AgNPs) mille-feuille structure electromagnetic interference shielding thermal conductivity Joule heating

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Unconventional Fluorescent and Multi-responsive Polyethyleneimine with LCST and UCST Behavior:Synthesis,Characterization and Biological Applications

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Chinese Journal of Polymer Science 2024年第6期42卷 826-837页

作者： Feng-Ming Yin Li-Li Wu Shu-Sheng Li Xiao-na pan Xiao-Li Zhu Xu-Bao Jiang Xiang Zheng Kong College of Chemistry and Chemical Engineering University of JinanJinan250022China Shandong Institute for Product Quality Inspection Jinan250102China College of Chemical Engineering Tianjin UniversityTianjin300072China

Non-aromatic fluorescent and multi-responsive materials,exhibiting inherent fluorescence emission and controlled phase change,have garnered significant attention in recent ***,the underlying interaction between their fluorescent properties and phase transition remains *** this study,we synthesized a series of catalyst-free aza-Michael addition-based polyethyleneimine(RFPEI)materials by reacting polyethyleneimine(PEI)with N-isopropyl acrylamide(NIPAM).The resulting RFPEI was comprehensively characterized,and demonstrated dual-phase transition behavior(LCST and UCST)in water,which could be finely tuned by adjusting its composition or external factors such as ***,upon UV irradiation(365 nm),RFPEI exhibited strong fluorescence *** further investigated the effects of NIPAM grafting percentage to PEI,polymer concentration,and pH on the LCST/UCST and fluorescent properties of RFPEI aqueous ***,we showcased the great potential of RFPEI as a versatile tool for physiological cell imaging,trace detection,and controlled release of *** study presents a novel class of stimuli-responsive fluorescent materials with promising applications in the field of biomedicine.

关键词： Polyethyleneimine Multi-responsiveness Intrinsic fluorescence emission Cell imaging Controlled drug release

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Machine learning-based prediction of pregnancy outcomes in couples with non-obstructive azoospermia using micro-TESE for ICSI: a retrospective cohort study

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生殖与发育医学(英文) 2024年第1期08卷 24-31页

作者： Jia Lei Chen Pei-Gen Chen Li-na Fang Cong Zhang Jing Chen pan-Yu Reproductive Medicine Center The Sixth Affiliated Hospital 3. Sun Yat-sen University 4. Guangzhou 510275 5. China

Objective: To develop a clinically applicable tool for predicting clinical pregnancy, providing individualized patient counseling, and helping couples with non-obstructive azoospermia (NOA) decide whether to use fresh or cryopreserved spermatozoa for oocyte insemination before microdissection testicular sperm extraction (mTESE).Methods: A total of 240 couples with NOA who underwent mTESE-ICSI were divided into two groups based on the type of spermatozoa used for intracytoplasmic sperm injection (ICSI): the fresh and cryopreserved groups. After evaluating several machine learning algorithms, logistic regression was selected. Using LASSO regression and 10-fold cross-validation, the factors associated with clinical pregnancy were ***: The area under the curves (AUCs) for the fresh and cryopreserved groups in the Logistic Regression-based prediction model were 0.977 and 0.759, respectively. Compared with various modeling algorithms, Logistic Regression outperformed machine learning in both groups, with an AUC of 0.945 for the fresh group and 0.788 for the cryopreserved ***: The model accurately predicted clinical pregnancies in NOA couples.

关键词： Cryopreserved spermatozoa Fresh spermatozoa Logistic regression Microdissection testicular sperm extraction

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Construction of CDKN2A-related competitive endogenous Rna network and identification of GAS5 as a prognostic indicator for hepatocellular carcinoma

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World Journal of Gastrointestinal Oncology 2024年第4期16卷 1514-1531页

作者： Yong pan Yi-Ru Zhang Ling-Yun Wang Li-na Wu Ying-Qiu Ma Zhou Fang Shi-Bo Li Department of Infectious Disease Zhoushan HospitalWenzhou Medical UniversityZhoushan 316021Zhejiang ProvinceChina State Key Laboratory for the Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of Zhejiang UniversityHangzhou 310003Zhejiang ProvinceChina Department of Infectious Disease The First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325035Zhejiang ProvinceChina

BACKGROUND Competitive endogenous Rna(ceRna)is an innovative way of gene expression modulation,which plays a crucial part in ***,the intricacy and behavioral characteristics of the ceRna network in hepatocellular carcinoma(HCC)remain *** To establish a cyclin dependent kinase inhibitor 2A(CDKN2A)-related ceRna network and recognize potential prognostic indicators for *** The mutation landscape of CDKN2A in HCC was first explored using the cBioPortal *** expression analysis was implemented between CDKN2Ahigh and CDKN2Alow expression HCC *** targeted microRnas were predicted by lncBasev3.0,and the targeted mRnas were predicted by miRDB,and Targetscan *** univariate and multivariate analysis were utilized to identify independent prognostic *** CDKN2A was frequently mutated and deleted in *** single-cell Rna-sequencing analysis revealed that CDKN2A participated in cell cycle *** CDKN2A-related ceRna network-growth arrest specific 5(GAS5)/miR-25-3p/SRY-box transcription factor 11(SOX11)was successfully ***5 was recognized as an independent prognostic biomarker,whose overexpression was correlated with a poor prognosis in HCC *** association between GAS5 expression and methylation,immune infilt-ration was ***,traditional Chinese medicine effective components targeting GAS5 were *** This CDKN2A-related ceRna network provides innovative insights into the molecular mechanism of HCC formation and ***,GAS5 might be a significant prognostic biomarker and therapeutic target in HCC.

关键词： Hepatocellular carcinoma Competitive endogenous Rna Molecular mechanism Prognosis Biomarker

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Des-Arg(9)bradykinin as a causal metabolite for autism spectrum disorder

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World Journal of Psychiatry 2024年第1期14卷 88-101页

作者： Zhong-Yu Huang Zi-pan Lyu Hong-Gui Li Hua-Zhi You Xiang-na Yang Cai-Hui Cha Center for Medical Research on Innovation and Translation Institute of Clinical MedicineGuangzhou First People’s HospitalSchool of MedicineSouth China University of TechnologyGuangzhou 510000Guangdong ProvinceChina School of Biological Sciences Nanyang Technological UniversityNanyang Ave 639798Singapore Department of Pediatrics First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina Department of Nutrition First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510405Guangdong ProvinceChina Department of Pediatric Traditional Chinese Medicine Clinic Guangzhou Women and Children's Medical CenterGuangzhou 510632Guangdong ProvinceChina Department of Psychology Guangzhou Women and Children's Medical CenterGuangzhou 510632Guangdong ProvinceChina

BACKGROUND Early diagnosis and therapeutic interventions can greatly enhance the developmental trajectory of children with autism spectrum disorder(ASD).However,the etiology of ASD is not completely *** presence of confounding factors from environment and genetics has increased the difficulty of the identification of diagnostic biomarkers for *** To estimate and interpret the causal relationship between ASD and metabolite profile,taking into consideration both genetic and environmental *** A two-sample Mendelian randomization(MR)analysis was conducted using summarized data from large-scale genome-wide association studies(GWAS)including a metabolite GWAS dataset covering 453 metabolites from 7824 European and an ASD GWAS dataset comprising 18381 ASD cases and 27969 healthy *** in plasma were set as exposures with ASD as the main *** causal relationships were estimated using the inverse variant weight(IVW)*** also performed leave-one-out sensitivity tests to validate the robustness of the *** on the drafted metabolites,enrichment analysis was conducted to interpret the association via constructing a protein-protein interaction network with multi-scale evidence from databases including Infinome,SwissTargetPrediction,STRING,and *** Des-Arg(9)-bradykinin was identified as a causal metabolite that increases the risk of ASD(β=0.262,SE=0.064,P_(IVW)=4.64×10^(-5)).The association was robust,with no significant heterogeneity among instrument variables(P_(MR Egger)=0.663,P_(IVW)=0.906)and no evidence of pleiotropy(P=0.949).Neuroinflammation and the response to stimulus were suggested as potential biological processes mediating the association between Des-Arg(9)bradykinin and *** Through the application of MR,this study provides practical insights into the potential causal association between plasma metabolites and *** findings offer perspectives for the discovery of diagnos

关键词： Des-Arg(9)bradykinin Autism spectrum disorder Mendelian randomization Metabolite Enrichment analysis

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数字社会主义:问题域与理论流脉

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开放时代 2024年第5期 144-164页

作者：潘娜中国社会科学院大学历史学院中国社会科学院当代中国研究所

数字社会主义是后金融危机时代西方世界兴起的一股新思潮,业已从“加州意识形态”的新话语转变为左翼建构资本主义替代方案的新主张,其理论走向大致形成三条脉络:一是挖掘20世纪20年代以来“社会主义经济计算大辩论”的理论资源,重新探... 详细信息

数字社会主义是后金融危机时代西方世界兴起的一股新思潮,业已从“加州意识形态”的新话语转变为左翼建构资本主义替代方案的新主张,其理论走向大致形成三条脉络:一是挖掘20世纪20年代以来“社会主义经济计算大辩论”的理论资源,重新探讨社会主义经济计划的可行性;二是聚焦马克思关于机器化大生产的经典论断,围绕“一般智力”等核心要件重塑数字权力关系,创建新型数字公地;三是从扬弃资本主义信用制度出发,建构基于区块链技术禀赋的代码治理系统。根本而言,目前种种数字社会主义模型囿于左翼固有局限,是一种充满技术乌托邦色彩的激进改良主义,不构成对资本主义生产方式和生产关系的完全替代。不过,数字社会主义在西方内部出现,揭示了数字金融资本主义的深刻危机,展现了这种危机所蕴含的变革因素,为非西方世界创造人类文明新形态提供了一种来自“他者”的比照和启示。

关键词：加州意识形态数字社会主义资本主义替代方案

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MCSTransWnet:A new deep learning process for postoperative corneal topography prediction based on raw multimodal data from the Pentacam HR system

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Medicine in Novel Technology and Devices 2024年第1期21卷 53-63页

作者： nan Cheng Zhe Zhang Jing pan Xiao-na Li Wei-Yi Chen Guang-Hua Zhang Wei-Hua Yang College of Biomedical Engineering Taiyuan University of TechnologyTaiyuan 030600ShanxiChina Shenzhen Eye Hospital Jinan UniversityShenzhen Eye InstituteShenzhen 518040GuangdongChina Department of Intelligence and Automation Taiyuan UniversityTaiyuan 030032ShanxiChina Graphics and Imaging Laboratory University of GironaGirona 17004Spain

This work provides a new multimodal fusion generative adversarial net(GAN)model,Multiple Conditions Transform W-net(MCSTransWnet),which primarily uses femtosecond laser arcuate keratotomy surgical parameters and preoperative corneal topography to predict postoperative corneal topography in astigmatism-corrected *** MCSTransWnet model comprises a generator and a discriminator,and the generator is composed of two *** first sub-generator extracts features using the U-net model,vision transform(ViT)and a multi-parameter conditional module branch.The second sub-generator uses a U-net network for further image *** discriminator uses the pixel discriminator in ***,most GAN models are convolutional neural networks;however,due to their feature extraction locality,it is difficult to comprehend the relationships among global ***,we added a vision Transform network as the model branch to extract the global *** is normally difficult to train the transformer,and image noise and geometric information loss are ***,we adopted the standard U-net fusion scheme and transform network as the generator,so that global features,local features,and rich image details could be obtained *** experimental results clearly demonstrate that MCSTransWnet successfully predicts postoperative corneal topographies(structural similarity=0.765,peak signal-to-noise ratio=16.012,and Fréchet inception distance=9.264).Using this technique to obtain the rough shape of the postoperative corneal topography in advance gives clinicians more references and guides changes to surgical planning and improves the success rate of surgery.

关键词： Deep learning Generative adversarial networks Corneal topography Transformer W-net U-net Medical imaging Multimodal fusion

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