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CLINICAL SIGNIFICANCE OF SERUM CYTOKINES IL-1β, sIL-2R, IL-6, TNF-α,AND IFN-νIN ACUTE CORONARY SYNDROME

Chinese Medical Sciences Journal 2004年第2期19卷 120-124页

作者： Yan-niwang Shao-minChe Ai-qunMa CardiovascularDepartmentofFirstHospital Xi＇anJiaotongUniversityXi＇an710061

Objectives To explore serum cytokines levels (including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-ν) and their significance in patients with acute coronary syndrome (ACS) and the subsequent follow-ups, with attempt to estimate the role of various serum inflammatory markers in the diagnosis and assessment of ACS. Methods The study population include 40 patients with acute myocardial infarction (AMI), 40 patients with unstable angina pectoris (UAP), and 40 controls. Among the 80 patients, 60 patients attended a follow up 4 months later. Serum inflammatory markers including IL-1β, sIL-2R, IL-6, TNF-α, and IFN-νwere measured by enzyme linked immunosorbent assay. ResultsSerum IL-1β, sIL-2R, IL-6, TNF-αwere significantly higher in AMI group or UAP group compared to the con-trol group and became significantly lower 4 months later in the follow-up patients. Serum levels of IFN-νshows no signifi-cant difference between AMI group or UAP group and controls, also showing no significant change when measured in follow up patients. There was no correlation between serum creatine kinase-MB isoenzyme levels and serum inflammatory markers either in UAP or AMI group. Furthermore, when divided into two subgroups using Wagner’s QRS scoring system in the AMI group, there is no difference of each serum inflammatory marker between ≤6 scores group and > 6 scores group. Conclusion Serum levels of certain inflammatory markers may have some diagnostic value for ACS, and can be a us-eful marker reflecting disease stability.

关键词： acute coronary syndrome inflammation cytokine

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Focal adhesion kinase antisense oligodeoxynucleotides inhibit human pulmonary artery smooth muscle cells proliferation and promote human pulmonary artery smooth muscle cells apoptosis

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Chinese Medical Journal 2005年第1期118卷 20-26页

作者： LINChun-long ZHANGZhen-xiang XUYong-jian niwang CHENShi-xin RespiratoryDepartment TongjiHospitalTongjiMedicalSchoolHuazhongUniversityofScienceandTechnologyWuhan430030China RespiratoryDepartment TongjiHospitalTongjiMedicalSchoolHuazhongUniversityofScienceandTechnologyWuhan430030China

Background Pulmonary artery smooth muscle cell (PASMC) proliferation plays an important role in pulmonary vessel structural remodelling At present, the mechanisms related to proliferation of PASMCs are not clear Focal adhesion kinase (FAK) is a widely expressed nonreceptor protein tyrosine kinase Recent research indicates that FAK is implicated in signalling pathways which regulate cytoskeletal organization, adhesion, migration, survival and proliferation of cells Furthermore, there are no reports about the role of FAK in human pulmonary artery smooth muscle cells (HPASMCs) We investigated whether FAK takes part in the intracellular signalling pathway involved in HPASMCs proliferation and apoptosis, by using antisense oligodeoxynucleotides (ODNs) to selectively suppress the expression of FAK protein Methods Cultured HPASMCs stimulated by fibronectin (40 μg/ml) were passively transfected with ODNs, sense FAK, mismatch sense and antisense FAK respectively Expression of FAK, Jun NH2 terminal kinase (JNK), cyclin dependent kinase 2 (CDK 2) and caspase 3 proteins were detected by immunoprecipitation and Western blots Cell cycle and cell apoptosis were analysed by flow cytometry In addition, cytoplasmic FAK expression was detected by immunocytochemical staining Results When compared with mismatch sense group, the protein expressions of FAK, JNK and CDK 2 in HPASMCs decreased in antisense FAK ODNs group and increased in sense FAK ODNs group significantly Caspase 3 expression upregulated in HPASMCs when treated with antisense ODNs and downregulated when treated with sense ODNs When compared with mismatch sense ODNs group, the proportion of cells at G 1 phase decreased significantly in sense ODNs group, while the proportion of cells at S phase increased significantly In contrast, compared with mismatch sense ODNs group, the proportion of cells at G 1 phase was increased significantly in antisense FAK ODNs group The level o

关键词： human pulmonary artery smooth muscle cells · focal adhesion kinase · proliferation · apoptosis Respiratory

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