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Expression and effect of TLR4 in rats with diabetic nephropathy

Asian Pacific Journal of Tropical Medicine 2013年第8期6卷 635-639页

作者： Si-Yang Liu Xiang-zhi nie Wen-Yang Zhou Jing Chen Department of Orthopaedics Renmin Hospital of Wuhan University Department of Nephrology Wuhan No.1 Hospital

Objective: To observe the expression of TLR4 in kidney tissue of rats with diabetic nephropathy and discuss the role of TLR4 in the occurrence and development of the diabetic nephropathy. Methods: A total of 60 clean male SD rats were selected and randomly divided into the modeling group and control group after 1 week of breeding, including 30 rats in each group. Biochemical indices as well as the protein expression of TLR4 were observed and compared between two groups at 2 w, 4 w, 6 w, 8 w and 12 w after the modeling, and the correlation between TLR4 and each biochemical indexes was analyzed. Results: Rats in the modeling group had higher levels of blood glucose, 24-hour urine protein and blood urea nitrogen after the modeling, and showed the increase in the serum creatinine, kidney/body weight ratio, CRP and serum TNF-毩 at 4w after the modeling, with the significant difference compared to results of the control group ( P <0.05). The cross-section area and mean volume of glomerulus in the modeling group at 4 w, 6 w, 8 w and 12 w were significantly higher than those in the control group, with the statistically significant difference ( P <0.05). The expression of TLR4 at each time point in the control group was relatively low. Rats in the modeling group had the high expression of TLR4 in kidney's glomerular basement membrane, proximal convoluted tubule and renal interstitial area since 2 w, with the significant difference compared to the control group ( P <0.05). The expression in rats of the modeling group was higher than the one of the control group since the 2nd week. As the time flied, its expression increased, with the statistically significant difference between two groups ( P <0.05). There was certain correlation between the protein expression of TLR4 and the increased serum titer of 24hour urine protein excretion, serum creatinine, CRP and TNF-毩. Conclusions: TLR4 may activate the immuno-inflammatory reactions to play a role in the occurrence and development

关键词： Diabetic nephropathy TLR4 Glomerulus

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The role of m6A modification in the biological functions and diseases

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Signal Transduction and Targeted Therapy 2021年第3期6卷 763-778页

作者： Xiulin Jiang Baiyang Liu zhi nie Lincan Duan Qiuxia Xiong zhixian Jin Cuiping Yang Yongbin Chen Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences&Yunnan Province Kunming Institute of Zoology650223 KunmingYunnanChina Kunming College of Life Science University of Chinese Academy of Sciences100049 BeijingChina Kunming Medical University 650500 KunmingChina Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences650223 KunmingYunnanChina

N6-methyladenosine(m6A)is the most prevalent,abundant and conserved internal cotranscriptional modification in eukaryotic RNAs,especially within higher eukaryotic cells.m6A modification is modified by the m6A methyltransferases,or writers,such as METTL3/14/16,RBM15/15B,ZC3H3,VIRMA,CBLL1,WTAP,and KIAA1429,and,removed by the demethylases,or erasers,including FTO and *** is recognized by m6A-binding proteins YTHDF1/2/3,YTHDC1/2 IGF2BP1/2/3 and HNRNPA2B1,also known as"readers".Recent studies have shown that m6A RNA modification plays essential role in both physiological and pathological conditions,especially in the initiation and progression of different types of human *** this review,we discuss how m6A RNA methylation influences both the physiological and pathological progressions of hematopoietic,central nervous and reproductive *** will mainly focus on recent progress in identifying the biological functions and the underlying molecular mechanisms of m6A RNA methylation,its regulators and downstream target genes,during cancer progression in above *** propose that m6A RNA methylation process offer potential targets for cancer therapy in the future.

关键词： m6A diseases removed

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Genome-Wide Expression Profile of Maize Root Response to Phosphorus Deficiency Revealed by Deep Sequencing

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Journal of Integrative Agriculture 2014年第6期13卷 1216-1229页

作者： SU Shun-zhong WU Ling LIU Dan LU Yan-li LIN Hai-jian ZHANG Shu-zhi SHEN Ya-ou LIU Hai-lan ZHANG zhi-ming RONG Ting-zhao ZHANG Xiao TIAN Yue-hui nie zhi GAO Shi-bin Key Laboratory of Biology and Genetic Improvement of Maize in Southwest Region Ministry of Agriculture/Maize Research InstituteSichuan Agricultural University

Phosphorus （P） is one of the three primary macronutrients that are required in large amounts for plant growth and development. To better understand molecular mechanism of maize and identify relevant genes in response to phosphorus deficiency, we used Solexa/Illumina＇s digital gene expression （DGE） technology to investigate six genome-wide expression profiles of seedling roots of the low-P tolerant maize inbred line 178. DGE studies were conducted at 6, 24 and 72 h under both phosphorus deficient and sufficient conditions. Approximately 3.93 million raw reads for each sample were sequenced and 6 816 genes exhibited significant levels of differential expressions in at least one of three time points in response to P starvation. The number of genes with increased expression increased over time from 6 to 24 h, whereas genes with decreased expression were more abundant at 72 h, suggesting a gradual response process for P deficiency at different stages. Gene annotations illustrated that most of differentially expressed genes （DEGs） are involved in different cellular and molecular processes such as environmental adaptation and carbohydrate metabolism. The expression of some known genes identified in other plants, such as those involved in root architecture, P metabolism and transport were found to be altered at least two folds, indicating that the mechanisms of molecular and morphological adaptation to P starvation are conserved in plants. This study provides insight into the general molecular mechanisms underlying plant adaptation to low-P stress and thus may facilitate molecular breeding for improving P utilization in maize.

关键词： maize phosphorus efficiency root digital gene expression

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Cyclin-dependent kinase 5 is required for suppressing D1-dependent signaling mediated through muscarinic 4 in isolated medium spiny neurons

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中国药理学与毒理学杂志 2018年第9期32卷 689-690页

作者： ZHOU Hu YANG Pei nie zhi-yong SHI Jing-shan WANG Li-yun LI Jin Beijing Institute of Pharmacology and Toxicology Beijing 100850 China Zunyi Medical University Zunyi 563000 China

OBJECTIVE Previous studies have demonstrated acetylcholine muscarinic 4(M4) receptor regulates DARPP-32 phosphorylation at Thr75 in isolated medium spiny neurons(MSNs),indicating antagonistic mechanism with D1 dependent signal cascade,but the exact molecular mechanisms remain *** this study,we investigated the roles of M4 receptor in modulation D1 dependent signal to integrate striatal DA inputs in isolated ***(1)Lentivirus technology was employed to genetically knock down the M4 receptor of MSNs;(2) Apomorphine(APO),acts as a dopamine receptor agonist,while SCH23390,acts as a selective antagonist for D1,were used to study the pharmacologically profiles with D1 receptor stimulation or blockade,*** the no subtype-selective muscarinic agonist oxotremorine M(OX) were used to show that mAchRs activation,in order to dissect the particular function of M4,a selective M4 antagonist,MT3 was used;(3) Intracellular cAMP production of MSNs was measured by using time resolved fluorescence resonance energy transfer detection method;(4) Laser confocal was used to explore the expression of M4 and D1 in MSNs;(5) Immunofluorescence cytochemistry and Western blotting were used to confirm the alteration of signaling molecular including P-CREB,DARPP-32 P-Thr34,DARPP-32 P-Thr75,cyclin-dependent kinase 5(CDK5) as wel as p25/35,which are involved in DA-dependent signaling *** Firstly,TR-FRET assay revealed APO(10-2 mol·L^(-1))significantly increased the level of intracellular cAMP(vs control,n=3,P<0.01),also Western blotting results showed that APO(10-6 mol · L^(-1))increased DARPP-32 Thr34 phosphorylation(vs control,n=3,P<0.01),and these effect were reversed by D1 receptor antagonist SCH23390(vs APO,n=3,P<0.01).Interestingly,we confirmed that OX(10-6 mol · L^(-1)) down-regulated APO-induced DARPP-32 Thr34 phosphorylation(vs APO,n=3,P<0.01),due to its effects on DARPP-32 phosphorylation at *** results presented the antagonistic mechanis

关键词： acetylcholine M4 receptor dopamine D1 receptor DARPP32 phosphorylation cyclin-dependent kinase 5

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Medium-term and Long-term Momentum and Contrarian Effects on China during 1994-2004

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Journal of Modern Accounting and Auditing 2007年第2期3卷 63-69页

作者： DU Xing-qiang nie zhi-ping School of Management Xiamen University Xiamen 361005 China

We examine momentum and contrarian effects in China＇s stock market during 1994-2004 and find that no medium momentum effects exist. Meanwhile, contrarian strategy works effectively over the horizon of 18-36 months. 1-month gap for avoiding bid-ask bounce and lead-lag effect make no considerable change to our empirical results. Transaction costs seem to have no significant impact on contrarian strategies＇ profit.

关键词： medium-term momentum long-term momentum contrafian effects transaction costs

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Fourier-shape-based reconstruction of rock joint profile with realistic unevenness and waviness features

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Journal of Central South University 2019年第11期26卷 3103-3113页

作者： nie zhi-hong WANG Xiang HUANG Dong-liang ZHAO Lian-heng School of Civil Engineering Central South UniversityChangsha 410075China Key Laboratory of Heavy-haul Railway Engineering Structure(Ministry of Education) Central South UniversityChangsha 410075China

Rock joint shape characteristics,waviness and unevenness play essential but distinct roles in shear mechanism of rock *** study presents a novel method to generate virtual rock joint profiles with realistic waviness and unevenness ***,joint profiles are obtained by 3D laser scanning ***,quantification of waviness and unevenness is conducted by traditional method,including digital filtering technique and roughness parameter ***,the discrete Fourier transform(DFT)method is employed to analyze the joint *** representative Fourier shape descriptors(D3,D8)for characterization of waviness and unevenness are ***,the inverse discrete Fourier transform(IDFT)is adopted to reconstruct the joint profiles with random values of phase angles but prescribed amplitudes controlled by D3 and *** traditional method is then applied to the reconstructed joint profiles to examine statistically the relationships between D3 and D8 and parameters RL of waviness and unevenness,*** results show that larger D8 tends to result in larger waviness while higher D3 tends to increase *** charts for estimation of waviness and unevenness with different pairs of D3 and D8 are also provided to facilitate implementation of random joint reconstruction.

关键词： rock joint profile joint shape analysis discrete Fourier transform joint reconstruction

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关于射影平坦的拟爱因斯坦度量的结构

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西南师范大学学报（自然科学版） 2010年第3期35卷 50-56页

作者：聂智重庆文理学院数学与统计学院重庆永川402160

针对拟Einstein流形的Hilbert第四问题给出了具有常flag曲率的射影平坦的多项式(α,β)-度量F=α1+∑ni=1aiβiαi的一种构作方法,得到了生成元ξ对F结构及其空间特征的影响.其中α是Riemann度量,β是1-形式.

关键词：拟Einstein流形生成元射影平坦多项式(α,β)-度量度量结构

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关于Minkowski空间中超曲面的曲率

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西南师范大学学报（自然科学版） 1999年第4期24卷 391-396页

作者：聂智重庆师范高等专科学校数学系永川402168

利用肌Minkowski空间中超曲面f的整体法向县场和Y-度量对应的Riemann第一基本形式，研究了f的法曲率、旗曲率以及Riemann超曲面的截面曲率．

关键词：整体法向量场 Minkowski空间超曲面曲率

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习近平关于意识形态工作重要论述的三重维度

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理论视野 2024年第8期294卷 25-31页

作者：聂智湖南工商大学马克思主义学院

习近平关于意识形态工作重要论述就新时代坚持和发展什么样的中国特色社会主义意识形态、怎样坚持和发展中国特色社会主义意识形态这一重大理论和实践命题进行深入阐述,提出意识形态工作定位论、形势论、任务论、主体论及立场论,强调制... 详细信息

习近平关于意识形态工作重要论述就新时代坚持和发展什么样的中国特色社会主义意识形态、怎样坚持和发展中国特色社会主义意识形态这一重大理论和实践命题进行深入阐述,提出意识形态工作定位论、形势论、任务论、主体论及立场论,强调制度守正、理论开新、舆论强基、网络固本、育新培元,实现意识形态领域的正本清源、革故鼎新和凝心聚力,既有系统完备的理论形态,也有不断创新的实践样态,是推动新时代意识形态建设的根本遵循。

关键词：习近平意识形态认识方法实践

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Finsler流形中测地线与全测地子流形的几个性质

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西南师范大学学报（自然科学版） 1999年第2期24卷 145-150页

作者：聂智西南师范大学数学系重庆400715

证明了Finsler流形中陈联络下的测地线与平均曲率下测地线的一致性,并指出(M,F)中测地线在对应(M,gY)中也是测地线;同时给出了Finsler流形中有关全测地子流形的性质,指出了Finsler全测地与对应Riemann全测地的关系.

关键词：平均曲率测地线全测地子流形 Finsler流形

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