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Effect of basic fibroblast growth factor on the proliferation, migration and phenotypic modulation of airway smooth muscle cells

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Chinese Medical Journal 2008年第5期121卷 424-429页

作者： ZOU Hui nie xiu-hong ZHANG Yi HU Mu ZHANG Yu Alex Cell Therapy Centre Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Pulmonary Disease Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Thoracic Surgery Xuanwu Hospital Capital Medical University Beijing 100053 China

Background Proliferation, cell migration and phenotypic modulation of airway smooth muscle cells （ASMCs） are important features of airway remodelling in asthma. The precise cellular and molecular mechanisms that regulate ASMCs proliferation, migration and phenotypic modulation in the lung remain unknown. Basic fibroblast growth factor （bFGF）, a highly specific chemotactic and mitogenic factor for many cell types, appears to be involved in the development of airway remodelling. Our study assessed whether bFGF directly stimulates the proliferation, migration and phenotypic modulation of ASMCs. Methods Confluent and growth arrested human ASMCs were treated with human recombinant FGF. Proliferation was measured by BrdU incorporation and cell counting. Migration was examined using Boyden chamber apparatus. Expressions of smooth muscle （sm）-α-actin and sm-myosin heavy chain （MHC） isoform 1 were determined by RT-PCR and Westem blot analysis. Results It was found that hrbFGF （10 ng/ml）, when added to ASMCs, induced a significant increase in BrdU uptake and cell number by ASMCs as compared to controls and a significant increase in ASMCs migration with respect to controls. The mRNA and protein expressions of sm-α-actin and sm-MHC in ASMCs that were stimulated with hrbFGF decreased with respect to controls. Conclusion It appears that bFGF can directly stimulate proliferation and migration of ASMCs, however, the expressions of cells＇ contractive phenotype decreased.

关键词： asthma basic fibroblast growth factor airway remodelling airway smooth muscle cells

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Comparison of Composition and Diversity of Bacterial Microbiome in Human Upper and Lower Respiratory Tract

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Chinese Medical Journal 2017年第9期130卷 1122-1124页

作者： Zhi-hong Feng Qin Li Si-Ran Liu Xiao-Nan Du Chen Wang xiu-hong nie Wei Wang Sun Ying Department of Respiratory Medicine Beijing Xuanwu Hospital Capital Medical University Beijing 100053 China Department of Immunology and The Research Centre of Microbiome School of Basic Medical Sciences Capital Medical University Beijing 100069 China Department of Respiratory Disease Capital Medical University Beijing 100069 China China-Japan Friendship Hospital National Clinical Research Center for Respiratory Diseases Beijing 100029 China

INTRODUCTIONMicrobiome residing in the airways and parenchymal tissues, as a biological barrier of respiratory tract, plays an important role in maintaining the normal functions of individual＇s respiratory system and preventing the invasion and colonization of exogenous pathogens.H1 Changes of microbial community may result in the occurrence and progression of infectious pneumonia and acute exacerbation of chronic diseases, through disordering commensal microorganisms and increasing invasive ability of pathogens.F21 Traditional methods for detecting bacteria normally rely on culture technique, which have a number of critical problems, including time-consuming and relatively low positive rate of detection. In the last 1 0 years, 16S rRNA gene sequencing technique has been thought to be more comprehensive and reliable than those of culture-dependent methods for monitoring bacterial microbiome in skin, organs, and tracts quantitatively and qualitatively.E31 In this case, choosing credible samples are critical to evaluate the community composition of microbiome in the host tracts, including respiratory tract. In the present study, we aimed to compare the composition and diversity of bacterial community structure in different samples collected from the individuals＇ airways using 16S rRNA gene sequencing technique.

关键词： 16S rRNA Gene Sequencing Bronchoalveolar Lavage Fluid Microbiota Respiratory Tract Sputum

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Advances in therapies using mesenchymal stem cells and their exosomes for treatment of peripheral nerve injury:state of the art and future perspectives

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Neural Regeneration Research 2025年第11期20卷 3151-3171页

作者： Fatima Aldali Chunchu Deng Mingbo nie hong Chen Department of Rehabilitation Medicine Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina Department of Orthopedics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei ProvinceChina

“Peripheral nerve injury”refers to damage or trauma affecting nerves outside the brain and spinal *** nerve injury results in movements or sensation impairments,and represents a serious public health *** severed peripheral nerves have been effectively joined and various therapies have been offered,recovery of sensory or motor functions remains limited,and efficacious therapies for complete repair of a nerve injury remain *** emerging field of mesenchymal stem cells and their exosome-based therapies hold promise for enhancing nerve regeneration and *** stem cells,as large living cells responsive to the environment,secrete various factors and *** latter are nano-sized extracellular vesicles containing bioactive molecules such as proteins,microRNA,and messenger RNA derived from parent mesenchymal stem *** have pivotal roles in cell-to-cell communication and nervous tissue function,offering solutions to changes associated with cell-based *** ongoing investigations,mesenchymal stem cells and mesenchymal stem cell-derived exosome-based therapies are in the exploratory stage.A comprehensive review of the latest preclinical experiments and clinical trials is essential for deep understanding of therapeutic strategies and for facilitating clinical *** review initially explores current investigations of mesenchymal stem cells and mesenchymal stem cell-derived exosomes in peripheral nerve injury,exploring the underlying ***,it provides an overview of the current status of mesenchymal stem cell and exosomebased therapies in clinical trials,followed by a comparative analysis of therapies utilizing mesenchymal stem cells and ***,the review addresses the limitations and challenges associated with use of mesenchymal stem cell-derived exosomes,offering potential solutions and guiding future directions.

关键词： clinical trials exosome extracellular vesicles mesenchymal stem cells nerve regeneration peripheral nerve injury pre-clinical experiments

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The cross-sectional and longitudinal association of the BODE index with quality of life in patients with chronic obstructive pulmonary disease

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Chinese Medical Journal 2009年第24期122卷 2939-2944页

作者： LIN Ying-xiang XU Wan-ning LIANG Li-rong PANG Bao-sen nie xiu-hong ZHANG Jie WANG hong LIU Yu-xiang WANG Dan-qi XU Zhen-yang WANG hong-wu ZHANG Hu-sheng HE Zheng-yi YANG Ting WANG Chen Beijing Institute of Respiratory Medicine Beijing Chaoyang Hospital Capital Medical University Beijing 100020 China Department of Epidemiology Biostatistics and Occupational Health McGill University Montrral Qurbec H3A 2T Canada Beijing Xuanwu Hospital 100035 China Beijing Tiantan Hospital 100050 China Capital Medical University Beijing Capital Medical University Beijing Beijing Sixth Hospital Beijing 100007 China Beijing Fuxing Hospital Capital Medical University Beijing 100038 China Jishuitan Hospital Beijing 100035 China Beijing Luhe Hospital Beijing 101100 China Coal General Hospital Beijing 100028 China Beijing Tongren Hospital Capital Medical University Beijing 100730 China Beijing Friendship Hospital Capital Medical University Beijing 100050. China

Background The body mass index, airflow obstruction, dyspnea, and exercise capacity （BODE） index was shown at predicting the risk of death, exacerbation and disease severity among patients with COPD, but few studies verified relationship between BODE index and health related quality of life （HRQoL） among Chinese COPD patients. The objective of this study was to evaluate the relationship between BODE index and HRQoL in cross-sectional and longitudinal association analyses. Methods A multi-center prospective cohort study was initially conducted in 491 stable COPD patients in Beijing, China. Health status （HRQoL） was assessed by St. George＇s Respiratory Questionnaire （SGRQ）; the BODE index was calculated for each patient; dyspnea was assessed using the 5-grade Medical Research Council dyspnea scale. Other measurements included socio-demographic, body mass index （BMI）, lung function test and 6-minute-walk test （6MWT）. Patients were then followed monthly for 12 months. Results Only 450 patients completed the 1-year follow up and were enrolled in our present analyses. Mean age was （65.2 ±10.6）years,＇ men 309 （68.7%）. The BODE index was categorized into 4 subgroups： 0-2, 3-4, 5-6 and 7-10. At baseline BODE index was gradually increased with baseline total SGRQ and SGRQ subscales （P trend 〈0.001）. For individual components of BODE index, with the decrease of airflow limitation, and 6MWD, and with the increase of Medical Research Council （MRC） dyspnea grade, total SGRQ and SGRQ subscales were increased correspondingly, P trend 〈0.05, respectively. Similar association patterns were found between baseline BODE index and its individual components and mean SGRQ scores at the end of 1-year follow up. By multiple linear regression analyses, baseline BODE index was not only significantly associated with SGRQ score at baseline but also with SGRQ score at the end of 1-year follow up after adjustment for age, male, current smoking, 13s being 0.434 and 0.378, respectively. Conclusions

关键词： chronic obstructive pulmonary disease BODE index health-related quality of life St. George's RespiratoryQuestionnaire

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非重症及重症脓毒症患者外周血单核细胞炎性因子分泌及Toll样受体4表达的研究

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中国急救医学 2011年第3期31卷 247-250页

作者：滕国杰聂秀红首都医科大学宣武医院呼吸科北京100053

目的观察革兰阴性菌感染的非重症及重症脓毒症患者内毒素（LPS）刺激后外周血单核细胞炎性因子分泌及TLR4的表达.方法以我院ICU革兰阴性菌感染所致脓毒症52例患者为研究对象,28例非重症脓毒症患者,24例重症脓毒症患者,选取30例健康对... 详细信息

目的观察革兰阴性菌感染的非重症及重症脓毒症患者内毒素（LPS）刺激后外周血单核细胞炎性因子分泌及TLR4的表达.方法以我院ICU革兰阴性菌感染所致脓毒症52例患者为研究对象,28例非重症脓毒症患者,24例重症脓毒症患者,选取30例健康对照者.所有人均晨起空腹采血,分离外周血单核细胞,将细胞加入1 μg/mL细菌LPS培养24 h后流式细胞仪检测单核细胞表面TLR4的表达,同时应用放射免疫法测定外周血单核细胞培养液中TNF-α、IL-10的浓度.结果 ①重症脓毒症患者单核细胞培养上清液中TNF-α的浓度低于非重症脓毒症患者及健康对照组（P〈0.05）,但IL-10浓度高于其他两组（P〈0.05）,IL-10/TNF-α比值明显高于非重症脓毒症患者及健康对照组（P〈0.01）.②三组患者外周血单核细胞LPS刺激后,细胞表面TLR4表达差异无统计学意义（P〉0.05）.结论重症脓毒症患者外周血单核细胞体外给予LPS刺激后,促炎因子表达较非重症脓毒症组下降,且伴有抗炎因子表达的上调,IL-10/TNF-α比值明显升高,显示重症脓毒症患者存在更加严重的免疫抑制现象,但这种现象不能单纯用细胞膜表面TLR4的表达解释,其可能存在更复杂的机制.

关键词：脓毒症外周血单核细胞肿瘤坏死因子-α(TNF-α) 白细胞介素-10(IL-10) Toll样受体4(TLR4)

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国产哌拉西林-舒巴坦与进口哌拉西林-他唑巴坦治疗下呼吸道感染的成本-效果分析

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中国新药杂志 2008年第8期17卷 693-696页

作者：雒志明畅怡聂秀红首都医科大学宣武医院呼吸内科首都医科大学宣武医院呼吸内科北京100053

目的:评价国产哌拉西林-舒巴坦与进口哌拉西林-他唑巴坦治疗下呼吸道细菌感染的成本-效果,促进临床治疗下呼吸道感染药物合理应用。方法:采用随机、单盲、对照试验设计,60例下呼吸道细菌感染患者分为试验组和对照组各30例,分别静滴哌拉... 详细信息

目的:评价国产哌拉西林-舒巴坦与进口哌拉西林-他唑巴坦治疗下呼吸道细菌感染的成本-效果,促进临床治疗下呼吸道感染药物合理应用。方法:采用随机、单盲、对照试验设计,60例下呼吸道细菌感染患者分为试验组和对照组各30例,分别静滴哌拉西林-舒巴坦2.5 g和哌拉西林-他唑巴坦4.5 g,q8h,疗程均为7~14 d。结果:试验组和对照组成本分别为574.5,1 337.4元,有效率分别为83.3%,86.7%(P>0.05),平均成本-效果比分别为6.89,15.73;对照组相对于试验组的增量成本-效果比为206.19。结论:哌拉西林-舒巴坦和哌拉西林-他唑巴坦治疗下呼吸道感染疗效与安全性相当,但前者具有明显的药物经济学优势。

关键词：下呼吸道细菌感染哌拉西林-舒巴坦哌拉西林-他唑巴坦成本-效果分析

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Improving aircraft nose wheel steering stability based on control continuation method

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Science China Technological Sciences 2025年第3期68卷 262-281页

作者： Qiaozhi YIN Dexu KONG Jiawei WANG hong nie Xiaohui WEI National Key Laboratory of Helicopter Aeromechanics Key Laboratory of Fundamental Science for National Defense-Advanced Design Technology of Flight Vehicle AVIC Nanjing Engineering Institute of Aircraft Systems State Key Laboratory of Mechanics and Control for Aerospace Structures

Aircraft steering instability during the taxiing process sometimes occurs resulting from the unstable bifurcation phenomena of the nonlinear steering dynamic system, which may lead to severe accidents. In this study, the control parameter design of an aircraft nose wheel steering control system based on the control continuation method is proposed to reduce the number of unstable bifurcation points and enlarge the system stable region area in a certain parameter domain. Thus, the aircraft turning performance can be improved greatly. First, a nonlinear aircraft rollout dynamic model is constructed, and the aircraft ground steering bifurcation characteristics are obtained under the open-loop steering control system. Then, the bifurcation performances under three closed-loop nose wheel steering controllers based on the feedback control method are compared, and the control parameter of the quadratic nonlinear controller with a better bifurcation control effect is selected as a second bifurcation parameter to analyze the directional system stability via two-parameter bifurcation analysis. Further, the influence of a crosswind on the aircraft ground steering bifurcation performance is studied for various control parameter values. Then, the control effects of a traditional proportional-integral-derivative(PID) nose wheel steering control law and the designed feedback controller are compared and analyzed. Finally, a semi-physical experiment of the aircraft nose wheel steering system is carried out, and different control parameter values are selected for test verification, which can further verify the control effect of the designed nonlinear feedback controller. The results indicate that the feedback control method is unable to vary the system bifurcation types, but appropriate control parameter design can effectively shrink the system instability region and even eliminate the unstable Hopf bifurcation points under some specific working conditions, improving the aircraft grou

关键词： aircraft taxiing dynamics and stability nose wheel steering control system control continuation steering bifurcation characteristics quadratic feedback control

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人KRAS真核表达载体的构建及生物学功能鉴定

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军事医学 2020年第1期44卷 60-63页

作者：王艺霖聂秀红首都医科大学宣武医院呼吸与危重症医学科北京100053

目的构建带FLAG标签的Kirsten鼠肉瘤病毒癌基因(Kirsten rat sarcoma viral oncogene,KRAS)真核表达载体,获得其表达产物并鉴定同源二聚体。方法应用PCR技术从人乳腺文库中扩增出KRAS全长编码区基因,将其克隆到pCMV-FLAG2B载体中,BamHⅠ... 详细信息

目的构建带FLAG标签的Kirsten鼠肉瘤病毒癌基因(Kirsten rat sarcoma viral oncogene,KRAS)真核表达载体,获得其表达产物并鉴定同源二聚体。方法应用PCR技术从人乳腺文库中扩增出KRAS全长编码区基因,将其克隆到pCMV-FLAG2B载体中,BamHⅠ、XhoⅠ双酶切及测序得到阳性克隆;利用重组质粒转染人胚肾293T细胞,以SDS-PAGE和免疫印迹鉴定表达情况;免疫共沉淀检测FLAG2B-KRAS与Myc-KRAS的相互作用。结果双酶切和基因测序显示,FLAG2B-KRAS真核表达载体构建成功;SDS-PAGE和免疫印迹结果表明,FLAG2B-KRAS转染人胚肾293T细胞后成功表达;免疫共沉淀结果显示,FLAG2B-KRAS与Myc-KRAS在蛋白水平上具有相互作用,能形成同源二聚体,证明其具有生物学活性。结论成功构建FLAG2B-KRAS真核表达载体,为进一步探讨KRAS在肿瘤分子生物学中的作用及靶向治疗奠定了实验基础。

关键词： Kirsten鼠肉瘤病毒癌基因真核表达同源二聚体

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58例咳嗽变异型哮喘的临床分析

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首都医科大学学报 2009年第6期30卷 841-844页

作者：侯会玲赵洪芬聂秀红首都医科大学宣武医院呼吸科

目的鉴别诊断咳嗽变异型哮喘(cough variant asthma,CVA)与慢性咳嗽。方法对112例慢性咳嗽患者经询问病史,摄X线胸片,做肺功能检查(支气管激发试验)和过敏原筛查。检查结果做卡方检验。结果112例慢性咳嗽患者中有58例确诊为CVA,其X线胸... 详细信息

目的鉴别诊断咳嗽变异型哮喘(cough variant asthma,CVA)与慢性咳嗽。方法对112例慢性咳嗽患者经询问病史,摄X线胸片,做肺功能检查(支气管激发试验)和过敏原筛查。检查结果做卡方检验。结果112例慢性咳嗽患者中有58例确诊为CVA,其X线胸片无异常改变,支气管激发试验均阳性,过敏原皮试阳性率为86.2%(50/58);42例存在诱因,其过敏原皮试阳性率为74.5%(38/42),其中79%(30/38)能检测到与诱因相关的过敏原;无诱因者的皮试阳性率为75%(12/16)。其他54例慢性咳嗽患者中X线胸片正常者34例,肺纹理增重者17例、有肺气肿征3例;支气管激发试验全部阴性,其中3例用力呼气1秒率(FEV1/FVC)<70%及用力呼气1秒量(FEV1)<80%;非CVA组过敏原皮试阳性率为16.7%(9/54);41例有诱因,其过敏原检测阳性率为12.2%(5/41),无诱因者皮试阳性率为30.8%(4/13)。经卡方检验,2组患者发病诱因(χ2=0.180,P=0.672)差异无统计学意义;而X线胸片无异常者(χ2=26.151,P=0.000)、支气管激发试验阳性率(χ2=112,P=0.000)、过敏原皮试阳性率(χ2=54.248,P=0.000)和无诱因病例中过敏原皮试阳性率(χ2=5.673,P=0.017)2组差异均有统计学意义。结论本组CVA患者的过敏原与诱因有一定的因果关系,无诱因的病例中大多数能检测出过敏原。支气管激发试验及过敏原筛查是诊断CVA的重要方法。

关键词：慢性咳嗽咳嗽变异型哮喘 X线胸片支气管激发试验过敏原皮试

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慢性阻塞性肺疾病的营养问题

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中国康复理论与实践 2009年第5期15卷 416-418页

作者：聂秀红首都医科大学宣武医院呼吸内科北京市100053

慢性阻塞性肺疾病(COPD)是一种以气流受限为特征的疾病,气流受限常呈进行性,并与有害气体颗粒与气体引起的炎症反应有关。COPD作为一种常见病,是目前世界上致死的第4大主要原因。在美国,45岁以上的人群中,COPD的患病率高达10%,与心血管... 详细信息

慢性阻塞性肺疾病(COPD)是一种以气流受限为特征的疾病,气流受限常呈进行性,并与有害气体颗粒与气体引起的炎症反应有关。COPD作为一种常见病,是目前世界上致死的第4大主要原因。在美国,45岁以上的人群中,COPD的患病率高达10%,与心血管疾病、创伤的病死率逐年下降比较,COPD病死率逐步上升,每年支付的医疗费用高达150亿美元。研究表明,COPD患者营养不良的发生率为25%－65%,随着病情的发展,一旦出现呼吸衰竭,则营养不良进一步加重。有些学者认为,营养不良、免疫低下和严重感染是COPD患者重要的致病因素,[第一段]

关键词：慢性阻塞性肺疾病(COPD) 营养评估治疗

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