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Effects of low pH and aluminum stresses on common beans (Phaseolus vulgaris) differing in low-phosphorus and photoperiod responses

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Frontiers in Biology 2009年第4期4卷 446-452页

作者： hai nian Cunyi YANG He HUANG Hideaki MATSUMOTO College of Agriculture South China Agricultural UniversityGuangzhou 510642China Research Institute for Bioresources Okayama University Kurashiki 710-0046Japan

Using common beans differing greatly in the response to photoperiod and low-phosphorus(P)stress,we investigated their responses to acidity and aluminum(Al)toxicity and the relationship between Al tolerance and organic acid exudation under Al or low P stress.A genotype Ginshi was found to be sensitive to low pH *** exposed to pH 4.5,serious curvature in the root tips of *** was observed;however,it was completely corrected by the application of 5 or 10μmol/L AlCl 3;increasing calcium(Ca)could ameliorate Al toxicity,but it could not correct root curvature at pH *** beans showed significant differences in both root growth and Al tolerance,and the varieties from the Andes were more tolerant to Al toxicity than those from the Mesoamerican *** the presence of 50μmol/L AlCl 3,all the common bean genotypes exuded citrate,and a significant difference in the amounts of citrate was observed among *** genotypes originated in the Mesoamerica tended to release more citrate than other origins in the presence of *** P-inefficient genotype DOR364 exuded more citrate than the P-efficient genotype G19833 in the presence of 50μmol/L AlCl 3,whereas no organic acids were detected in root exudates under low-P stress.A reduction of citrate exudation in the DOR364,but a slight increase of citrate exudation in the G19833,was observed under Al stress after they were exposed to 6-d P *** results suggest that different low-P or Al tolerance in common beans might not be associated with organic acid exudation.

关键词： common bean low-pH aluminum organic acid exudation phosphorus deficiency

An Octave-Spanning,Kerr-Lens Mode-Locked Ti:sapphire Oscillator with Double-Chirped Mirrors and BaF_(2)

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Chinese Physics Letters 2012年第11期29卷 88-90页

作者： ZHANG Long HAN hai-nian ZHANG Qing WEI Zhi-Yi Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190

We demonstrate an octave-spanning Kerr-lens mode-locked Ti:sapphire laser with double-chirped mirrors(DCMs),BaF_(2) wedges and plate,which generates laser pulses in the spectrum range from 600 nm to 1200 nm at-45 dB below the maximum and the duration of 8.5 fs at the repetition rate of 80 *** average output power is 100 mW under 5 W pump *** carefully adjusting the insertion of BaF_(2) wedges and the optimization position of Ti:sapphire crystal in the cavity,we can obtain the smooth swings in the interferometric autocorrelation trace due to good third-order dispersion compensation.

关键词： pump wedge repetition

Effects on Genome Constitution and Novel Cell Wall Formation Caused by the Addition of 5RS Rye Chromosome to Common Wheat

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Journal of Integrative Plant Biology 2008年第4期50卷 503-509页

作者： Zhi-Jun Cheng Minoru Murata Sodmergen Xiao-Mei Li hai nian Jian-Min Wan National Key Facility for Crop Gene Resources and Genetic Improvement institute of Crop Science Chinese Academy of Agriculture Science Beijing 100081 China Research/nstitute for Bioresources Okayama University Kurashiki 710-0046 Japan College of Life Science Peking University Beijing 100871 China State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany the Chinese Academy of Sciences Beijing 100093 China South China Agncultural University Guangzhou 300098 China

The cytological instability of common wheat-rye addition lines was investigated in the present study. The chromosome numbers of almost all addition lines were considerably stable, but those of CS ＋ 5R were very variable. The rye chromosome added in this line was found to be much shorter than expected. Fluorescent in situ hybridization with 5S rDNA and the centromere-speciflc probes clearly revealed that the short rye chromosome contains only a short arm of chromosome 5R （5RS）. In this line, chromosome numbers of both 5RS and common wheat were changeable. The chromosome numbers ranged from 2n = 36 to 2n = 44 in the cells carrying two 5RS, and ranged from 2n = 31 to 2n = 44 in one 5RS cells. In addition to the chromosome instability, the muIticells wrapped in a sac-like structure were frequently observed in the root meristematic tissues of CS ＋5RS after the enzyme treatment for chromosome preparation. Genomic in situ hybridization with rye DNA as a probe showed that all cells in sacs investigated were at the interphase stage and contained one or two 5RS chromosomes. An electron microscopic analysis revealed that the cells of CS＋5RS, particularly in sacs, have abnormal （irregular and curved） cell walls. These results indicate that 5RS has （a） specific factor（s） influencing the cell wall development as well as the genome stability.

关键词： addition lines cell wall chromosome instability common wheat rye.

Fine mapping of an adult-plant resistance gene to powdery mildew in soybean cultivar Zhonghuang 24

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The Crop Journal 2022年第4期10卷 1103-1110页

作者： Qian Zhou Bingzhi Jiang Yanbo Cheng Qibin Ma Qiuju Xia Ze Jiang Zhandong Cai hai nian The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642GuangdongChina The Guangdong Provincial Laboratory of Lingnan Modern Agricultural Science and Technology South China Agricultural UniversityGuangzhou 510642GuangdongChina The Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangdong Provincial Key Laboratory of Crops Genetics and Improvement Crops Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510642GuangdongChina Beijing Genomics Institute(BGI)Education Center University of Chinese Academy of SciencesShenzhen 518083GuangdongChina

Powdery mildew(PM),caused by the fungus Microsphaera diffusa,causes severe yield losses in soybean[Glycine max(L.)Merr.]under suitable environmental *** resistance genes and developing resistant cultivars may prevent soybean PM *** this study,analysis of F_(1),F_(2),and F8:11 recombinant inbred line(RIL)populations derived from the cross between Zhonghuang 24(ZH24)and Huaxia 3(HX3)indicated that adult-plant resistance(APR)to powdery mildew in the soybean cultivar(cv.)ZH24 was controlled by a single dominant locus.A high-density genetic linkage map of the RIL population was used for fine *** APR locus in ZH24 was mapped to a 281-kb genomic region on chromosome *** 283 susceptible plants of another F2 population,the candidate region was finemapped to a 32.8-kb genomic interval flanked by the markers InDel14 and Gm16_*** interval harbored five genes,including four disease resistance(R)-like genes,according to the Williams 82.a2.v1 reference *** real-time PCR assays of candidate genes revealed that the expression levels of Glyma.16g214300 and Glyma.16g214500 were changed by *** infection and might be involved in disease ***_B13 showed all-stage resistance(ASR)to PM in soybean *** allelism test in the F2 segregating population from the cross of ZH24 × B13 suggested that the APR locus Rmd_ZH24 and the ASR locus Rmd_B13 may be allelic or tightly *** results provide a reference marker-assisted selection in breeding programs.

关键词： Glycine max Powdery mildew Adult-plant resistance Gene Fine mapping

Effects　of　Ecological　Conditions　of　the　Northeast　of　China　on　Protein，Oil　and　Protein＋　Oil　Content　in　Soybean　Seeds

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Journal of Northeast Agricultural University(English Edition) 1996年第1期3卷 1-6页

作者： nian hai Wang Jingling Yang Qingkai(Northeast Agricultural University,Harbin 150030 PRC) Northeast Agricultural University Harbin 150030 PRC

Twenty five soybean strains of similar growth period were grown at eight locations simultanously in three northeast provinces of *** of the experimental sites differed markedly in temperature,precipitation, soil fertility and length of growing *** and oil contents were detelmined to study the effects of ecological conditions on soybean chemical composition. The results showed that the average protein and protein + oil contents were higher at the south locations and lower at north locations, but there was no consistent variation for oil content,and there was no significant difference among most of the sites. Oil content of strains was highest when planted in Jiling. Combined analyses of variance indicated that there existed significant genotype,location and G×E interactions effects. F values of G×E interactions for all traits,although significant at 0.01 level of probability, were extremely smaller than those for location and *** suggested that G×E interaction effects were relatively less important than both genotyp and location *** correlations of the performance of genotypes,on single ehtry -basis,among eight locations were all positive for protein and protein+ oil and most of correlation coefficients were significant at 0.01 level of *** indicated that the relative performance of genotypes at each location was similar. In contrast,most of the correlation coefficients for oil content among the eight locations were not ***,G×E interactions should be considered in breeding for high oil soybeans,as it may lead to the instability of genotypes and affect the rankings of genotypes when soybeans arc grown in diverse environments.

关键词： soyberan chemical traits ecological conditions

Earthworm rather than biochar and sodium silicate addition increased bacterial diversity in mining areas subjected to chemical fertilization

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Biochar 2019年第4期1卷 365-374页

作者： Tengxiang Lian Ling Ma Yajun Zeng Scott XChang Qibin Li Xiaoyang Chen hai nian The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityNo.483 Wushan RoadGuangzhou 510642GuangdongPeople’s Republic of China The Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510642GuangdongPeople’s Republic of China Guangdong Key Laboratory for Innovative Development and Utilization of Forest Plant Germplasm College of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642GuangdongPeople’s Republic of China Department of Renewable Resources University of AlbertaEdmontonAB T6G 2E3Canada

The addition of biochar,sodium silicate,or earthworm is a feasible practice to repair soils disturbed by mining activities,and the reclamation is largely based on the alteration of the diversity and structure of soil *** objectives of this study were to assess the relative importance of these supplements on soil bacterial community diversity and structure in reclaimed mine areas.A field experiment with soybean was carried out in mining areas to assess the efficiency of nitrogen,phospho-rus and potassium(NPK)fertilizers plus those supplements on soil bacterial community structure and diversity by the 16S rRNA sequencing *** chemical properties were analyzed to their effects on the bacterial community *** results showed that the application of nitrogen,phosphorus and potassium(NPK)fertilizers significantly increased bacterial diversity,and a further increase was observed in NPK plus biochar,sodium silicate or earthworm ***,a higher number of genera were found in the NPK plus biochar and NPK plus earthworm treatments than that in the control,NPK and NPK plus sodium silicate *** bacterial community was significantly associated with nutrients,such as carbon(C)and nitrogen(N).Moreover,soil organic carbon(SOC)and pH were the most dominant factors in shaping the soil bacterial community structure and *** data indicate that the addition of earthworms to soil rather than biochar and sodium silicate was the best strategy to mitigate the detrimental effects of mining activities on soil bacterial diversity.

关键词： Mine reclamation Biochar Soil organic carbon Bacterial community Illumina MiSeq sequencing

Fine mapping and genetic analysis of resistance genes,Rsc18,against soybean mosaic virus

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Journal of Integrative Agriculture 2022年第3期21卷 644-653页

作者： LIU Sang-lin CHENG Yan-bo MA Qi-bin LI Mu JIANG Ze XIA Qiu-ju nian hai The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources South China Agricultural UniversityGuangzhou 510642P.R.China The Key Laboratory of Plant Molecular Breeding of Guangdong Province College of AgricultureSouth China Agricultural UniversityGuangzhou 510642P.R.China Maize Research Institute Jilin Academy of Agricultural SciencesGongzhuling 130033P.R.China Beijing Genomics Institute(BGI)-Shenzhen Shenzhen 518086P.R.China

Soybean mosaic virus(SMV) affects seed quality and production of soybean(Glycine max(L.) Merr.) ***18 is one of the dominant SMV strains in South China,and accession Zhonghuang 24 displayed resistance to *** F_(1),F_(2) and 168 F_(11) recombinant inbred lines(RILs) population derived from a hybridization between Zhonghuang 24(resistant,R) and Huaxia 3(susceptible,S) were used in this *** to the segregation ratios of the F_(2) generation(3 R:1 S) and the recombinant inbred lines(RILs) population(1 R:1 S),one dominant locus may regulate the resistance to SC18 in Zhonghuang *** using composite interval mapping(CIM),Rsc18 was mapped to a 415.357-kb region on chromosome *** candidate genes,including one NBS-LRR type gene and two serine/threonine protein type genes,were identified according to the genetic annotations,which may be related to the resistance to *** q RT-PCR demonstrated that these genes were up-regulated in the R genotype compared to the *** conclusion,the findings of this research enhanced the understanding about the R genes at the Rsc18 ***,our results will provide insights for designing molecular markers to improve marker-assisted selection and developing new varieties with resistance to SC18.

关键词： soybean mosaic virus(SMV) fine mapping recombinant inbred lines(RILs) resistance gene

A Wide Spaced Femtosecond Ti:Sapphire Frequency Comb at 15 GHz by a Fabry–Pérot Filter Cavity

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Chinese Physics Letters 2013年第10期30卷 97-100页

作者： HOU Lei HAN hai-nian ZHANG Jin-Wei LI De-Hua WEI Zhi-Yi Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190

We realize a wide spaced frequency comb by using an external low-fineness Fabry–Pérot(F-P)cavity to filter few-cycle laser pulses from a Kerr-lens mode-locked Ti:sapphire laser at the fundamental repetition rate of 350 *** spacing as wide as 15 GHz with spectrum covered from 690 nm to 710 nm is demonstrated,corresponding to a filter multiple of about *** scanning transmission peaks after the F-P cavity with cavity lengths are also simulated numerically,and the results are in agreement with the experiment.

关键词： Femto Fabry filter

Subwavelength optical localization with toroidal excitations in plasmonic and Mie metamaterials

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InfoMat 2021年第5期3卷 577-597页

作者： Ruisheng Yang Jing Xu nian-hai Shen Fuli Zhang Quanhong Fu Junjie Li Hongqiang Li Yuancheng Fan Key Laboratory of Light-field Manipulation and Information Acquisition Ministry of Industry and Information Technology and School of Physical Science and TechnologyNorthwestern Polytechnical UniversityXi'anChina Ames Laboratory and Department of Physics and Astronomy Iowa State UniversityAmesIowaUSA Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijingChina School of Physics Science and Engineering Tongji UniversityShanghaiChina The Institute of Dongguan-Tongji University DongguanGuangdongChina

Since the performance of electronic circuits is becoming rather limited in face of intensively increasing of amount of information and related operations,alloptical processing offers a promising strategy for future information *** would benefit a great deal if the all-optical processing could be implemented within the developed electronic chips of nanoscale *** that it is highly desirable to break the diffraction limit of light for achieving effective light manipulations with deep subwavelength structures compatible with the state-of-the-art nanofabrication *** is of fundamental importance to get subwavelength optical localization,that is,squeeze light wave into subwavelength space for achieving freely manipulating of light *** review summarizes the development in realizing subwavelength optical localization by exciting toroidal mode in photonic *** toroidal excitations in plasmonic metamaterials and Mie resonant metamaterials,in 3D structures and planar metamaterials,with single or few layers in spectral regime from microwave to optical frequencies are *** on the discussion on the configurations of toroidal excitations,the recent development on toroidalrelated optical scattering control actively manipulates the toroidal excitations,and promising applications are further investigated and highlighted.

关键词： dielectric metamaterials metamaterials subwavelength optics toroidal metasurfaces toroidal moment