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Molecular characterization, tissue and developmental expression profiles of cryptochrome genes in wing dimorphic brown planthoppers, nilaparvata lugens

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Insect Science 2016年第6期23卷 805-818页

作者： Jing-Jing Xu Gui-Jun Wan Ding-bang Hu Juan He fa-Jun Chen Xian-Hui Wang Hong-Xia Hua Wei-Dong Pan Beijing Key Laboratory of Bioelectromagnetics Institute of Electrical Engineering Chinese Academy of Sciences Beijing University of Chinese Academy of Sciences Beijing Department of Entomology College of Plant Protection Nanjing Agricultural University Nanjing Collge of Plant Science & Technology Huazhong Agricultural University Wuhan State College of Plant Protection Northwest Agriculture and Forest University Yangfing Shaanxi State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of Zoology Chinese Academy of Sciences Beijing China

Cryptochromes （CRYs） are blue and UV light photoreceptors, known to play key roles in circadian rhythms and in the light-dependent magnetosensitivity of insects. Two novel cryptochrome genes were cloned from the brown planthopper, and were given the designations of Nlcryl and Nlcry2, with the accession numbers KM108578 and KM108579 in GenBank. The complementary DNA sequences ofNlcryl andNlcry2 are 1935 bp and 2463 bp in length, and they contain an open reading frame of 1629 bp and 1872 bp, encoding amino acids of 542 and 623, with a predicted molecular weight of 62.53 kDa and 70.60 kDa, respectively. Well-conserved motifs such as DNA-photolyase and faD-binding-7 domains were observed in Nlcry1 and Nlcry2. Phylogenetic analysis demonstrated the proteins of Nlcry1 and Nlcry2 to be clustered into the insect＇s cryptochrome 1 and cryptochrome 2, respectively. Quantitative polymerase chain reaction showed that the daily oscillations of messenger RNA （mRNA） expression in the head of the brown planthopper were mild for Nlcryl, and modest for Nlcry2. Throughout all developmental stages, Nlcryl and Nlcry2 exhibited extreme fluctuations and distinctive expression profiles. Cryptochrome mRNA expression peaked immediately after adult emergence and then decreased subsequently. The tissue expression profiles of newly emerged brown planthopper adults showed higher expression levels of CRYs in the head than in the thorax or abdomen, as well as significantly higher levels of CRYs in the heads of the macropterous strain than in the heads of the brachypterous strain. Taken together, the results of our study suggest that the two cryptochrome genes characterized in the brown planthopper might be associated with developmental physiology and migration.

关键词： cryptochrome migration molecular characterization nilaparvata lugens wing dimorphism

Experimental study on MSW gasification and melting technology

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Journal of Environmental Sciences 2007年第11期19卷 1398-1403页

作者： XIAO Gang JIN Bao-sheng ZHONG Zhao-ping CHI Yong ni Ming-jiang CEN Ke-fa XIAO Rui HUANG Ya-ji HUANG He Key Laboratory of Clean Coal Power Generation and Combustion Technology of Ministry of Education School of Energy and Environment Southeast University Nanjing 210096 China State Key Laboratory of Clean Energy Utilization Zhejiang University Hangzhou 310027 China College of Life Science and Pharmaceutical Engineering Nanjing University of Technology Nanjing 210009 China

In order to develop municipal solid waste （MSW） gasification and melting technology, two preliminary experiments and a principle integrated experiment were fulfilled respectively. The gasification characteristics of MSW were studied at 500-750℃ when equivalence ratio （ER） was 0.2--0.5 using a fluidized-bed gasifier. When temperature was 550-700℃ and ER was 0.2--0.4, low heat value （LHV） of syngas reaches 4000-12000 kJ/Nm^3. The melting characteristics of fly ash were investigated at 1100-1460℃ using a fixed-bed furnace. It was proved that over 99.9% of dioxins could be decomposed and most heavy-metals could be solidified when temperature was 1100-1300℃. The principle integrated experiment was carried out in a fluidized-bed gasification and swirl-melting system. MSW was gasified etticiently at 550-650℃, swirl-melting furnace maintains at 1200-1300℃ stably and over 95% of fly ash could be caught by the swirl-melting furnace. The results provided much practical experience and basic data to develop MSW gasification and melting technology.

关键词： municipal solid waste (MSW) fluidized-bed gasification swirl-melting characteristics

Application of the ISCST3 model for predicting PCDD/F concentrations in agricultural soil in the vicinity of a MSWI plant in China

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2008年第3期9卷 373-380页

作者： Meng-xia XU Jian-hua YAN Sheng-yong LU Xiao-dong LI Tong CHEN Ming-jiang ni Hui-fen DAI Ke-fa CEN State Key Laboratory of Clean Energy Utilization Institute for Thermal Power Engineering Zhejiang University Hangzhou 310027 China School of Medicine Zhejiang University Hangzhou 310058 China

Based on the Industrial Source Complex Short-Term Version 3 （ISCST3） model, a simplified modeling approach was developed to predict concentrations of congeners of polychlorinated-p-dioxins and dibenzofurans （PCDD/Fs） of agricultural soil, within a radius of 3 kin from a municipal solid waste incinerator （MSWI） plant after its 4-year operation in Hangzhou, China. Comparisons were made between the measured and estimated congener-specific concentrations and the international-toxic equivalent （I-TEQ） values of soil samples with respect to distance from the stack. The results indicate that the predictions of soil PCDD/F concentrations and K-TEQ values were generally lower than their observations, and that the higher the degree of underestimation seems, the greater the further downwind one gets. Nevertheless, most of the predictions were in good agreement with the trend of measured ones and were within a factor of ten for samples located within 1 kin of the plant. Besides, analysis of contributions of various deposition pathways confirms that in addition to wet particle deposition, the dry gaseous deposition is essential for realistic prediction of PCDD/F depositions to soil, especially for tetra- and penta-chlorinated dioxins.

关键词： Agricultural soil Dispersion model ISCST3 Municipal solid waste incinerator (MSWI) Polychlorinated-p-dioxins and dibenzofurans (PCDD/Fs)

妇炎消胶囊联合头孢地尼胶囊对慢性盆腔炎患者血液流变学和血清炎性因子的影响

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现代生物医学进展 2024年第7期24卷 1349-1352页

作者：王艳妮桑琳吕发辉张莉丁玉芹合肥市第二人民医院妇产科安徽合肥230011

目的:观察妇炎消胶囊联合头孢地尼胶囊对慢性盆腔炎患者血液流变学和血清炎性因子的影响。方法:选择2022年2月-2023年2月期间合肥市第二人民医院收治的慢性盆腔炎患者106例。按照双色球法将患者分为对照组和实验组,各为53例。对照组接... 详细信息

目的:观察妇炎消胶囊联合头孢地尼胶囊对慢性盆腔炎患者血液流变学和血清炎性因子的影响。方法:选择2022年2月-2023年2月期间合肥市第二人民医院收治的慢性盆腔炎患者106例。按照双色球法将患者分为对照组和实验组,各为53例。对照组接受头孢地尼胶囊,实验组在对照组的基础上接受妇炎消胶囊,均治疗14天。对比两组疗效、临床症状缓解、炎症因子、血液流变学和不良反应。结果:实验组的临床总有效率高于对照组(P0.05)。结论:妇炎消胶囊联合头孢地尼胶囊治疗慢性盆腔炎患者,可有效改善患者的临床症状,改善血液流变学,降低血清炎性因子水平,且无明显不良反应。

关键词：妇炎消胶囊头孢地尼胶囊慢性盆腔炎血液流变学炎性因子

Effects of mild/asymptomatic COVID-19 on semen parameters and sex-related hormone levels in men:a systematic review and meta-analysis

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Asian Journal of Andrology 2023年第3期25卷 382-388页

作者： bang-Wei Che Pan Chen Ying Yu Wei Li Tao Huang Wen-Jun Zhang Sheng-Han Xu Jun He Miao Liu Kai-fa Tang Department of Urology The Affiliated Hospital of Guizhou Medical UniversityGuiyang 550004China

Coronavirus disease 2019(COVID-19)has yet to be proven to alter male reproductive function,particularly in the majority of mild/asymptomatic *** purpose of this study was to explore whether mild/asymptomatic COVID-19 affects semen quality and sex-related hormone *** find suitable comparative studies,a systematic review and meta-analysis was done up to January 22,2022,by using multiple databases(Web of Science,PubMed,and Embase).Preferred Reporting Items for Systematic Reviews and Meta-Analyses(PRISMA)guidelines were used to identify and choose the ***-analysis was used to examine the semen parameters and sex-related hormones of mild/asymptomatic COVID-19 patients before and after *** effects of semen collection time,fever,and intensity of verification on semen following infection were also investigated.A total of 13 studies(n=770)were included in the analysis,including three case-control studies,six pre-post studies,and four single-arm studies.A meta-analysis of five pre-post studies showed that after infection with COVID-19,sperm concentration(I^(2)=0;P=0.003),total sperm count(I^(2)=46.3%;P=0.043),progressive motility(I^(2)=50.0%;Pnificant relationship between semen collection time after infection,inflammation severity,and semen parameter values,with fever having only bearing on semen ***,there was no significant difference in sex-related hormone levels before and after infection in mild/asymptomatic ***/asymptomatic COVID-19 infection had a significant effect on semen quality in the short *** is recommended to avoid initiating a pregnancy during this period of time.

关键词： coronavirus disease 2019 male infertility meta-analysis semen quality severe acute respiratory syndrome coronavirus 2

Effects of long-term grazing exclusion on vegetation structure,soil water holding capacity,carbon and nitrogen sequestration capacity in an alpine meadow on the Tibetan Plateau

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Journal of Mountain Science 2023年第3期20卷 779-791页

作者： YANG Yong-sheng ZHANG fa-wei XIE Xian-rong WANG Jun-bang LI Ying-nian HUANG Xiao-tao LI Hui-ting ZHOU Hua-kun Key Laboratory of Adaptation and Evolution of Plateau Biota and Key Laboratory of Restoration Ecology in Cold Region of Qinghai Province Northwest Institute of Plateau BiologyChinese Academy of SciencesXining 810008China Qinghai Haibei National Field Research Station of Alpine Grassland Ecosystem Xining 810008China Xi ning Municipal Beishan Forestry farm Xining 810003China Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China

Grazing exclusion is one of the primary management practices used to restore degraded grasslands on the Tibetan ***,to date,the effects of long-term grazing exclusion measures on the process of restoring degraded alpine meadows have not been *** this study,moderately degraded plots,in which the vegetation coverage was approximately 65%and the dominant plant species was Potentilla anserina L,with grazing exclusion for 2 to 23 years,were selected in alpine meadows of Haibei in Qinghai-Tibet *** coverage,plant height,biomass,soil bulk density,saturated water content,soil organic carbon(SOC)and total nitrogen(TN)were *** results were as follows:(1)With aboveground biomass and total saturated water content at 0-40 cm depth,the average SOC and TN contents in moderately degraded alpine meadows increased as a power function,and the plant height increased as a log function.(2)The average soil bulk density at 0-40 cm depth first decreased and then increased with increasing grazing exclusion duration,and the minimum value of 0.90 g·cm^(-3) was reached at 15.23 *** plant coverage,total belowground biomass at 0-40 cm depth,total aboveground and belowground biomass first increased and then decreased,their maximum values(80.49%,2452.92g·m^(-2),2891.06 g·m^(-2))were reached at 9.41,9.46 and 10.25 years,***-term grazing exclusion is apparently harmful for the sustainable restoration of degraded alpine *** optimal duration of grazing exclusion for the restoration of moderately degraded alpine meadows was 10 *** research suggests that moderate disturbance should be allowed in moderately degraded alpine meadows after 10years of grazing exclusion.

关键词： Long-term grazing exclusion Soil water holdingcapacity Soilcarbonand nitrogen sequestration Biomass Alpine meadow

Destruction of PCDD/Fs by gliding arc discharges

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Journal of Environmental Sciences 2007年第11期19卷 1404-1408页

作者： YAN Jian-hua PENG Zheng LU Sheng-yong DU Chang-ming LI Xiao-dong CHEN Tong ni Ming-jiang CEN Ke-fa State Key Laboratory of Clean Energy Utilization Institute for Thermal Power Engineering Zhejiang University Hangzhou 310027 China Environmental Science and Engineering Sun Yat-Sen University Guangzhou 510275 China

PCDD/Fs have been become a serious issue because of their toxicological effects and associated adverse health implications. In this study, the gliding arc plasma was tested for treatment of polychlorinated dibenzo-p-dioxins （PCDDs） and polychlorinated dibenzofurans （PCDFs）, which was synthesized from pentachlorophenol in atmospheric condition at 350℃ with or without the catalysis of CuCl2. From the experiment, we found that the destruction efficiency of PCDD/F homologues after gliding was discharge ranged from 25% to 79%. This result demonstrates that gliding arc plasma is an effective technology to decompose PCDDs/Fs in flue gas. A plausible degradation mechanism for PCDD/Fs by gliding arc was discussed. Finally, a multistage reactor structure of gliding arc was proposed to upgrade removal efficiency for PCDD/Fs.

关键词： PCDD/Fs destruction gliding arc discharges, multistage reactor

Frequency effect on p-nitrophenol degradation under conditions of strict acoustic and electric control

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Water Science and Engineering 2011年第1期4卷 74-82页

作者： Chang-ping ZHU Bo HUANG Qing-bang HAN Cai-hua ni Guang-jun ZHU Ming-hua LIU Ming-lei SHAN College of Computer and lnformation Engineering Hohai University Changzhou 213022 P. R. China State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering Hohai University Nanjing 210098 P. R. China College of Information and Electrical Engineering China University of Mining and Technology Xuzhou 221008 P. R. China School of Chemical and Material Engineering Jiangnan University Wuxi 214122 P. R. China

The process of decomposing p-nitrophenol （PNP） with power ultrasound requires strict control of acoustic and electric conditions. In this study, the conditions, including acoustic power and acoustic intensity, but not ultrasonic frequency, were controlled strictly at constant levels. The absorbency and the COD concentrations of the samples were measured in order to show the variation of the sample concentration. The results show significant differences in the trend of the solution degradation rate as acoustic power increases after the PNP solution （with a concentration of ll4 mg/L and a pH value of 5.4） is irradiated for 60 min with ultrasonic frequencies of 530.8 kHz, 610.6 kHz, 855.0 kHz, and 1130.0 kHz. The degradation rate of the solution increases with time and acoustic power （acoustic intensity）. On the other hand, the degradation rate of the solution is distinctly dependent on frequency when the acoustic power and intensity are strictly controlled and maintained at constant levels, The degradation rate of the PNP solution declines with ultrasonic frequencies of 530.8 kHz, 610.6 kHz, 855.0 kHz, and 1 130.0 kHz; the COD concentration, on the contrary, increase.

关键词： ultrasonic treatment frequency effect COD concentration p-nitrophenol

过渡金属比例对锂离子电池Lini_(x)Co_(y)Mn_(z)O_(2)层状材料电化学性能及热特性的影响

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1936-1950页

作者：梁晨童邦李师魏泽森孙金华王青松常州大学石油和化工行业动力电池安全与事故防控技术工程实验室常州213164 常州大学安全科学与工程学院常州213164 中国科学技术大学火灾科学国家重点实验室合肥230026 合肥国轩高科动力能源有限公司合肥230016

为了揭示Lini_(x)Co_(y)Mn_(z)O_(2)(NCM)的性能随过渡金属(TM)成分的变化规律,系统研究TM比例对不同NCM材料的结构、形貌、电化学性能和热行为的影响。镍含量的增加能够提高可逆容量,但速率性能和循环稳定性变差。在富镍NCM中,Li^(+)... 详细信息

为了揭示Lini_(x)Co_(y)Mn_(z)O_(2)(NCM)的性能随过渡金属(TM)成分的变化规律,系统研究TM比例对不同NCM材料的结构、形貌、电化学性能和热行为的影响。镍含量的增加能够提高可逆容量,但速率性能和循环稳定性变差。在富镍NCM中,Li^(+)的动力学抑制和电子导电性差是容量损失的主要原因。通过原位和非原位微量热法对不同NCM的热行为进行对比。结果表明,随着ni含量的降低,材料的热稳定性显著提高。通过对NCM中Li剩余量的分析,对相变相关的氧释放量进行评估。结果表明,富镍材料在脱锂后出现更严重的结构恶化,起始温度更低,热释放量更多。综合表征表明,Lini_(0.5)Co_(0.3)Mn_(0.2)O_(2)在电化学性能和安全性能间实现良好的平衡。

关键词：镍钴锰酸锂热稳定性结构演变三元材料锂离子电池安全