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The Obstacles and Potential Solution Clues of Prime Editing Applications in Tomato

BioDesign Research 2022年第1期2022卷 11-18页

作者： Tien Van Vu Ngan thi nguyen Jihae kim Swati Das Jinsu Lee Jae-Yean kim Division of Applied Life Science(BK21 FOUR Program) Plant Molecular Biology and Biotechnology Research CenterGyeongsang National UniversityJinju 660-701Republic of Korea National Key Laboratory for Plant Cell Biotechnology Agricultural Genetics InstituteKm 02Pham Van Dong RoadCo Nhue 1Bac Tu LiemHanoi 11917Vietnam Division of Life Science Gyeongsang National University501 Jinju-daeroJinju 52828Republic of Korea Nulla Bio Inc. 501 Jinju-daeroJinju 660-701Republic of Korea.

Precision genome editing is highly desired for crop *** recently emerged CRISPR/Cas technology offers great potential applications in precision plant genome engineering.A prime editing(PE)approach combining a reverse transcriptase(RT)with a Cas9 nickase and a“priming”extended guide RNA(gRNA)has shown a high frequency for precise genome modification in mammalian cells and several plant ***,the applications of the PE approach in dicot plants are still limited and *** designed and tested prime editors for precision editing of a synthetic sequence in a transient assay and for desirable alleles of 10 loci in tomato by stable *** data obtained by targeted deep sequencing also revealed only low PE efficiencies in both the tobacco and tomato *** assessment of the activities of the PE components uncovered that the fusion of RT to Cas9 and the structure of PE gRNAs(pegRNAs)negatively affected the cleaving activity of the Cas9 *** self-complementarity between the primer binding sequences(PBSs)and spacer sequence might pose risks to the activity of the Cas9 ***,modifying the pegRNA sequences by shortening or introducing mismatches to the PBSs to reduce their melting temperatures did not enhance the PE efficiency at the MADS-box protein(SlMBP21),alcobaca(SlALC),and acetolactate synthase 1(SlALS1)*** data show challenges of the PE approach in tomato,indicating that a further improvement of the PE system for successful applications is demanded,such as the use of improved expression systems for enriching active PE complexes.

关键词： CRISPR/Cas precise editing

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Efficacy of He-Ne Laser in Combination with Topical Clindamycin in the Treatment of Acne Vulgaris (AV)

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Journal of Cosmetics, Dermatological Sciences and Applications 2020年第3期10卷 134-139页

作者： Bui thi Van nguyen Ngoc oanh Đặng Văn Em Huynh thi Xuan Tam nguyen Lan Anh Pham thi Minh Phuong Dermatology-Venereology and Allergy 108 Central Military Hospital Hanoi Vietnam Dermatology-Venereology Department Bach Mai Hospital Hanoi Vietnam Pham Ngoc Thach University of Medicine Ho Chi Minh City Vietnam

Objective: To evaluate the efficacy of He-Ne laser in combination with topical clindamycin in the treatment of AV at 108 Military Central Hospital from Oct 2015 to Aug 2016. Subjects and Methods: 61 patients with AV were divided into 2 groups: Group 1: 31 patients were treated with He-Ne laser in combination with topical Clindamycin, Group 2: 30 patients were only treated with topical clindamycin. Laser HE-NE was applied 2 times/week for 6 weeks. Result: After 3 months of treatment Group 1: very good—51.6%, good—48.4%. Group 2: very good—30%, good—50% and moderate—20%. Conclusion: The combination of He-Ne laser with topical clindamycin shows more rapid clinical improvement compared to topical clindamycin alone in the treatment of mild and moderate AV.

关键词： Acne Vulgaris He-Ne Laser Clindamycin

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Upconverting nanoparticle-containing erythrocyte-sized hemoglobin microgels that generate heat,oxygen and reactive oxygen species for suppressing hypoxic tumors

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Bioactive Materials 2023年第4期22卷 112-126页

作者： Hanju kim Johyun Yoon Hwang Kyung kim Woo Tak Lee nguyen thi nguyen Xuan thien Le Eun-Hee Lee Eun Seong Lee Kyung Taek Oh Han-Gon Choi Yu Seok Youn School of Pharmacy Sungkyunkwan University2066 Seobu-roJangan-guSuwonGyeonggi-do16419Republic of Korea College of Pharmacy Korea University2511 Sejong-roSejong30019Republic of Korea Department of Biotechnology and Department of Biomedical-Chemical Engineering the Catholic University of Korea43 Jibong-roBucheon-siGyeonggi-do14662Republic of Korea College of Pharmacy Chung-Ang University84 Heukseok-roDongjak-guSeoul06974Republic of Korea College of Pharmacy Hanyang University55Hanyangdaehak-roSangnok-guAnsan15588Republic of Korea

Inspired by erythrocytes that contain oxygen-carrying hemoglobin(Hb)and that exhibit photo-driven activity,we introduce homogenous-sized erythrocyte-like Hb microgel(μGel)systems(5-6μm)that can(i)emit heat,(ii)supply oxygen,and(iii)generate reactive oxygen species(ROS;1O2)in response to near-infrared(NIR)laser *** μGels consist of Hb,bovine serum albumin(BSA),chlorin e6(Ce6)and erbium@lutetium upconverting nanoparticles(UCNPs;~35 nm)that effectively convert 808 nm NIR light to 660 nm visible *** Hb μGels are capable of releasing oxygen to help generate sufficient reactive oxygen species(^(1)O_(2))from UCNPs/Ce6 under severely hypoxic condition upon NIR stimulation for efficient photodynamic ***,the Hb μGels emit heat and increase surface temperature due to NIR light absorption by heme(iron protoporphyrin IX)and display photothermal *** changing the Hb/UCNP/Ce6 ratio and controlling the amount of NIR laser irradiation,it is possible to formulate bespoke Hb μGels with either photothermal or photodynamic activity or both in the context of combined therapeutic *** Hb μGels effectively suppress highly hypoxic 4T1 cell spheroid growth and xenograft mice tumors in vivo.

关键词： Hemoglobin microgel Upconversion Photothermal therapy Photodynamic therapy Hypoxic tumors Oxygen-generation

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Anticancer effects of saponin and saponin–phospholipid complex of Panax notoginseng grown in Vietnam

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Asian Pacific Journal of Tropical Biomedicine 2016年第9期6卷 795-800页

作者： Thu Dang kim Hai nguyen Thanh Duong nguyen Thuy Loi Vu Duc Thu Vu thi Hung Vu Manh Patcharee Boonsiri Tung Bui Thanh Department of Pharmacology and Clinical Pharmacy School of Medicine and PharmacyVietnam National University Department of Pharmaceutics and Pharmaceutical Technology School of Medicine and PharmacyVietnam National University Department of Pharmacology Hanoi University of Pharmacy Department of Pharmacognosy and Traditional Pharmacy School of Medicine and PharmacyVietnam National University Faculty of Biology VNU University of Science Key Laboratory of Enzyme and Protein Technology VNU University of Science Department of Pharmacology School of PharmacyLac Hong University Department of Biochemistry Faculty of MedicineKhon Kaen University

Objective:To evaluate the antitumor activity both in vitro and in vivo of saponin–phospholipid complex of Panax ***:The in vitro cytotoxic effect of saponins extract and saponin–phospholipid complex against human lung cancer NCI-H460 and breast cancer cell lines BT474 was examined using MTS *** in vivo evaluation of antitumor potential,saponin and saponin–phospholipid complex were administered orally in rats induced mammary carcinogenesis by 7,12-dimethylbenz(a)anthracene,for 30 ***:Our data showed that saponin–phospholipid complex had stronger anticancer effect compared to saponin *** IC50 values of saponin–phospholipid complex and saponin extract for NCI-H460 cell lines were 28.47 mg/m L and 47.97 mg/mL,respectively and these values for BT474 cells were 53.18 mg/mL and 86.24 mg/mL,*** vivo experiments,administration of saponin,saponin–phospholipid complex and paclitaxel(positive control) effectively suppressed 7,12-dimethylbenz(a) anthracene-induced breast cancer evidenced by a decrease in tumor volume,the reduction of lipid peroxidation level and increase in the body weight,and elevated the enzymatic antioxidant activities of superoxide dismutase,catalase,glutathione peroxidase in rat breast ***:Our study suggests that saponin extract from Panax notoginseng and saponin–phospholipid complex have potential to prevent cancer,especially breast cancer.

关键词： Panax notoginseng Saponin Saponin–phospholipid complex Breast cancer Antitumor

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Dynamic soil-structure interaction analysis in time domain based on a modified version of perfectly matched discrete layers

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Journal of Rock Mechanics and Geotechnical Engineering 2020年第1期12卷 168-179页

作者： Dong Van nguyen Dookie kim Department of Civil Engineering Kunsan National University558 Daehak-roGunsan-si54150Republic of Korea

Analysis of soil-structure interaction is commonly conducted by dividing the infinite domain of the soil into two domains:interior and exterior *** interior domain is bounded in a small region,while the exterior domain is replaced by artificial boundary *** choice of artificial boundary conditions is a critical issue in the analysis of soil-structure interaction *** matched discrete layer(PMDL)has been proved as a good approach for modeling the exterior *** this study,a modified version of the PMDLs,*** with analytical wavelengths(AW-PMDLs),is used in the soil-structure interaction analysis in time domain,which essentially can be regarded as an extension of the analysis in frequency domain,being previously proven to be *** verifications are *** results demonstrate that the proposed method performs well in the analysis of soilstructure interaction problems in time domain.

关键词： Soil-structure interaction Time domain Wave propagation Wavelength Infinite domain Perfectly matched discrete layer(PMDL)

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A study implementing real-time PCR to identify Strongyloides species of third-stage larvae in human stool samples from Southern Vietnam

Infectious Medicine

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Infectious Medicine 2023年第3期2卷 229-236页

作者： Le Duc Vinh nguyen kim Thach Huynh Hong Quang Do Nhu Binh Tran thi Duc Hanh nguyen Minh Toàn nguyen Trung Tuyen nguyen Thu Huong Department of Medical Parasitology Pham Ngoc Thach University of MedicineHo Chi Minh City 700000Vietnam Department of Fundamental Chemistry-Biochemistry Pham Ngoc Thach University of MedicineHo Chi Minh City 700000Vietnam Clinical Epidemiology Research Unit Institute of MalariologyParasitologyand Entomology Quy NhonBinh Dinh 55000Vietnam Department of Infectious Disease Military Hospital 103Hanoi 100000Vietnam Department of Epidemiology Hanoi University of Public HealthHanoi 100000Vietnam Department of Microbiology-Parasitology Hanoi University of Public HealthHanoi 100000Vietnam Department of Medicine University of Medicine and PharmacyVietnam National UniversityHanoi100000 Vietnam

Background:Strongyloidiasis,a neglected disease caused by intestinal nematodes of the genus,is endemic to tropical and subtropical areas such as *** methods only identify the genus,while DNA-molecular techniques are susceptible in Strongyloides *** study aims to determine the prevalence of dominant Strongyloides species among the population in Duc Hoa district,Long An,***:A cross-sectional study used 1190 stool specimens collected from July 2017 to November *** samples were transported within 2 h,stored at 2-8℃,and processed within 48 h for microscopy smear and culture at the Laboratory of Medical Parasitology,Pham Ngoc Thach University of Medicine(PNT).Then all positive samples with the above 2 methods were verified by real-time PCR ***-time PCR amplification was conducted at the Laboratory of Molecular Biology,***:Direct microscopy and modified Harada-Mori culture detected Strongyloides *** in 79/1190 sam-ples(6.6%).About 94.2%of the DNA samples were Strongyloides stercoralis,2.9%were co-infections with Strongy-loides ratti and ***,and 2.9%were patients with *** identity of 12/14 sequences was confirmed as *** with a high level of similarity(91.3%-100%)and over 98%for ***:DNA-molecular techniques and sequence analysis are highly suitable for identifying Strongyloides species isolated from stool *** is remarkable evidence of the presence of zoonosis *** disease in human,not just the known *** is likely to result in a certain proportion of people being infected by this animal-borne infectious pathogen.

关键词： Strongyloidiasis Strongyloides stercoralis Strongyloides ratti DNA-molecular techniques

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Ethanol extracts of Scutellaria baicalensis protect against lipopolysaccharide-induced acute liver injury in mice

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Asian Pacific Journal of Tropical Biomedicine 2015年第9期5卷 733-738页

作者： Hai nguyen Thanh Hue Pham thi Minh Tuan Anh Le Huong Duong thi Ly Tung nguyen Huu Loi Vu Duc Thu Dang kim Tung Bui Thanh School of Medicine and Pharmacy Vietnam National University Hanoi University of Pharmacy

Objective: To investigated the protective potential of ethanol extracts of Scutellaria baicalensis(S. baicalensis) against lipopolysaccharide(LPS)-induced liver injury. Methods: Dried roots of S. baicalensis were extracted with ethanol and concentrated to yield a dry residue. Mice were administered 200 mg/kg of the ethanol extracts orally once daily for one week. Animals were subsequently administered a single dose of LPS(5 mg/kg of body weight, intraperitoneal injection). Both protein and m RNA levels of cytokines, such as tumor necrosis factor alpha, interleukin-1β, and interleukin-6 in liver tissues were evaluated by ELISA assay and quantitative PCR. C yclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB protein levels in liver tissues were analyzed by western blotting. Results: Liver injury induced by LPS signifi cantly increased necrosis factor alpha, interleukin-1β, interleukin-6, cyclooxygenase-2, inducible nitric oxide synthase, and nuclear factor-κB in liver tissues. Treatment with ethanol extracts of S. baicalensis prevented all of these observed changes associated with LPS-induced injury in liver ***: Our study showed that S. baicalensis is potentially protective against LPS-induced liver injury in mice.

关键词： Scutellaria baicalensis Lipopolysaccharide Liver injury Cytokine Nuclear factor-κB

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Examination of n-T_(9) conditions required by N=50,82,126 waiting points in r-process

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Communications in Theoretical Physics 2021年第10期73卷 147-157页

作者： nguyen Duy Ly nguyen Ngoc Duy nguyen kim Uyen Vinh N T Pham Faculty of Fundamental Sciences Vanlang UniversityHo Chi Minh City 700000Vietnam Department of Physics Sungkyunkwan UniversitySuwon 16419Republic of Korea Department of Physics Ho Chi Minh City University of EducationHo Chi Minh City 700000Vietnam

We examined the conditions of neutron density(n) and temperature(T9) required for the N = 50, 82,and 126 isotopes to be waiting points(WP) in the r-process. The nuclear mass based on experimental data presented in the AME2020 database(AME and AME ± Δ) and that predicted using FRDM,WS4, DZ10, and KTUY models were employed in our estimations. We found that the conditions required by the N = 50 WP significantly overlap with those required by the N = 82 ones, except for the WS4 model. In addition, the upper(or lower) bounds of the n-T9 conditions based on the models are different from each other due to the deviations in the two-neutron separation *** standard deviations in the nuclear mass of 108 isotopes in the three N = 50, 82, and 126 groups are about rms = 0.192 and 0.434 Me V for the pairs of KTUY-AME and WS4-KTUY models,respectively. We found that these mass uncertainties result in a large discrepancy in the nn-T_(9) conditions, leading to significant differences in the conditions for simultaneously appearing all the three peaks in the r-process abundance. The newly updated FRDM and WS4 calculations can give the overall conditions for the appearance of all the peaks but vice versa for their old versions in a previous study. The change in the final r-process isotopic abundance due to the mass uncertainty is from a few factors to three orders of magnitude. Therefore, accurate nuclear masses of the r-process key nuclei, especially for 76 Fe,81Cu,127Rh,132Cd,192Dy, and 197Tm, are highly recommended to be measured in radioactive-ion beam facilities for a better understanding of the r-process evolution.

关键词： neutron capture r-process nuclear mass isotopic abundance precise mass measurement

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高管团队特征对企业绩效的影响研究 ——基于越南证券交易所服务业上市公司实证分析

高管团队特征对企业绩效的影响研究 —...

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作者：苏莹（TO thi kim oanh）西南大学

学位级别：硕士

自从Hambrick和Mason提出高层梯队理论以后,诸多学者开始对公司高管团队人口特征与企业绩效的关系进行研究。经过大量研读中国以及其他国家的学者相关研究后发现,高管团队特征对企业绩效存在一定的影响。越南加入WTO后,越南服务业快速发... 详细信息

自从Hambrick和Mason提出高层梯队理论以后,诸多学者开始对公司高管团队人口特征与企业绩效的关系进行研究。经过大量研读中国以及其他国家的学者相关研究后发现,高管团队特征对企业绩效存在一定的影响。越南加入WTO后,越南服务业快速发展,服务行业的增长是多种因素共同作用的结果。其中最重要的因素是竞争压力越来越大,企业要想生存就必须更加注重发展质量和效益、改善公司绩效、提升自己的能力,以适应当下经济发展的速度。本文基于高层梯队理论,以越南证券交易所上市公司作为研究对象,选取企业2016-2019年的数据样本,从高管团队特征的视角开始,围绕高管团队特征的年龄、受教育水平、性别、任期、高管团队规模5个方面,分析高管团队特征对企业绩效的影响机理,明晰不同高管团队特征对企业绩效影响,厘清不同高管团队特征与企业绩效关系的影响机理,在此基础上提出如何有效调整高管团队特征,引导高管团队最大限度地提升企业绩效的政策建议。本文具体研究内容有:（1）高管团队特征影响企业绩效的理论分析。在明晰相关概念的基础上,借鉴经典理论和学者们的观点,分析高管团队年龄、受教育水平、性别、任期、高管团队规模对企业环境绩效的影响机理,论述不同高管团队特征与企业环境绩效关系的影响作用,并提出研究假设。（2）高管团队特征对企业绩效影响机理、不同高管团队特征与企业绩效关系的影响作用的测度。在已有文献的研究基础之上,合理选择关键变量的度量方式,采集并整理相关数据,构建回归模型,对研究假设进行检验,并验证检验结果的稳健性。（3）总结研究结论。从高管团队特征来探讨提升企业绩效的主要途径,为企业和政府提出合理的管理决策和政策建议。本文的主要研究结论如下:（1）高管团队性别比例与企业绩效存在显著的关系,高管团队女性占比更高更利于企业绩效的提升。（2）高管团队年龄对企业绩效具有积极影响作用,中年龄的管理者有一定管理经验,高管团队平均年龄与企业绩效存在正相关关系。（3）高管团队受教育水平与企业绩效显著正相关,与大部分的研究结果相同,高管团队受教育水平越高,越能创造最佳的企业绩效。（4）高管团队任期对企业绩效存在显著的负面影响。（5）高管团队规模与企业绩效存在显著的正相关关系,企业规模代表企业在市场上的财务能力和竞争优势,大型高管团队企业将有许多机会扩展业务活动,并能提升行业和业务领域的竞争能力。通过实证研究结果来看,本文为越南服务行业企业组建高管团队提出了以下几点建议:一是提高高管团队的受教育水平,聘请高管人员过程中,受教育水平是一个很重要的考察指标,为了提升管理人员能力素质企业需要建立培训、学习型组织。二是企业需要考虑公司发展阶段来组建一个年龄结构合理的高管团队。三是高管团队的女性比例更高更利于企业绩效。因此,企业应多元化高管团队性别,也是给女性一个发挥自己的能力。四是要保持相对稳定的高管团队人数,高管人员规模越大更利于企业经营政策的有效性,但是也要需要思考符合自己公司以及经营领域的高管规模。五是控制好高管团队任期,考虑高管团队的当前组成情况以及团队将如何紧密合作。另外,本文也阐述了研究的不足,对此作出进一步的展望。

关键词：企业绩效高管团队特征越南服务业

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Lithium plating-free 1 Ah-level high-voltage lithium-ion pouch battery via ambi-functional pentaerythritol disulfate

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能源化学:英文版 2023年第8期83卷 229-238,I0007页

作者： Dung Tien Tuan Vu Jinsol Im Jae-Hee kim Jisoo Han Gyeong Jun Chung Giang thi Huong nguyen Junhyeok Seo Minjae kim Eui-Hyung Hwang Young-Gil Kwon Jae Wook Shin Kuk Young Cho Seung-Wan Song Department of Chemical Engineering&Applied Chemistry Chungnam National UniversityDaejeon 34134Republic of Korea Department of Materials Science&Chemical Engineering Hanyang UniversityAnsan 15588Republic of Korea Fine Chem Co.Ltd. Daejeon 34365Republic of Korea d CSIM Co.Ltd. Yesan 32400Republic of Korea Dongwha Electrolyte Nonsan 32925Republic of Korea

Elevating the charge cut-off voltage beyond traditional 4.2 V is a commonly accepted technology to increase the energy density of Li-ion batteries(LIBs) but the risk of Li-dendrites and fire hazard increases as well. The use of ambi-functional additive, which forms stable solid electrolyte interphase(SEI) simultaneously at both cathode and anode, is a key to enabling a dendrites-free and well-working high-voltage LIB. Herein, a novel ambi-functional additive, pentaerythritol disulfate(PEDS), at 1 wt% without any other additive is demonstrated. We show the feasibility and high impacts of PEDS in forming lithium sulfateincorporated robust SEI layers at NCM523 cathode and graphite anode in 1 Ah-level pouch cell under4.4 V, 25 °C and 0.1 C rate, which mitigates the high-voltage instability, metal-dissolution and cracks on NCM523 particles, and prevents Li-dendrites at graphite anode. Improved capacity retention of 83%after 300 cycles is thereby achieved, with respect to 69% with base electrolyte, offering a promising path toward the design of practical high-energy LIBs.

关键词： Lithium-ion pouch cell Lithium plating-free High-voltage Ambi-functional additive SEI layer

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