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Low- and high-dose hydrogen peroxide regulation of transcription factor NF-E2-related factor 2

Chinese Medical Journal 2010年第8期123卷 1063-1069页

作者： NING Jiao-lin MO Li-wen LAI xi-nan Department of Anesthesia Southwest Hospital Third Military Medical University Chongqing 400038 China Department of Nephrology People's Liberation Army Chengdu Military Area Command General Hospital Chengdu Sichuan 610083 China State Key Laboratory of Trauma Burns and Combined injury Institute of Surgery Research Department of Traumatic Surgery Daping Hospital Third Melitary Medical University Chongqing 400042 China

Background Reactive oxygen species （ROS） may play both physiological and pathophysiological roles. Transcription factor NF-E2-related factor 2 （Nrf2） regulates antioxidant response element （ARE）-mediated genes expression and coordinates induction of chemoprotective proteins in response to physical and chemical stresses. The exact role of Nrf2 in cellular responses to different levels of oxidative stresses remains unknown. Methods Rat pulmonary microvascular endothelial cells were cultured and treated with 0 mmol/L, 0.125 mmol/L, 0.25 mmol/L, 0.5 mmol/L, 1.0 mmol/L and 2.0 mmol/L hydrogen peroxide solution for 2 hours. Nrf2 gene expression was assayed by reverse transcription-PCR, Nrf2-ARE binding activity was assayed with electrophoretic mobility shift assay （EMSA）, and localization of Nrf2 was detected with immunohistochemistry. Results Low and moderate （0.125 mmol/L, 0.25 mmol/L and 0.5 mmol/L） doses hydrogen peroxide exposure of rat pulmonary microvascular endothelial cells led to the nuclear accumulation of Nrf2, increased activity of transcription regulation and up-regulation of ARE-medicated gene expression. In contrast, high doses of hydrogen peroxide （1 mmol/L 2 mmol/L） exposure of the cells led to the nuclear exclusion of Nrf2, decreased activity transcription regulation and down-regulation of ARE-mediated gene expression. Conclusion Low and moderate doses of hydrogen peroxide play protective roles by increasing transcription activity of Nrf2, whereas high- dose hydrogen peroxide plays a deleterious role by decreasing transcription activity of Nrf2.

关键词： pulmonary micro-vascular endothelial cells antioxidant responsive element, hydrogen peroxide NF-E2-related factor 2

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Factors influencing the surveillance of re-emerging intracranial infections in elective neurosurgical patients:A single-center retrospective study

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World Journal of Clinical Cases 2023年第28期11卷 6680-6687页

作者： Jiang-Long Wang xi-wen Wu Sheng-nan Wang Xuan Liu Bing xiao Yu Wang Jing Yu The First Operating Room The First Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina Department of Neurology The First Hospital of Jilin UniversityChangchun 130000Jilin ProvinceChina

BACKGROUND At present,many studies have reported the risk factors for postoperative intracranial reinfection,including age,sex,time to surgery,duration of postoperative catheterization,emergency procedures,type of disease and cerebrospinal fluid leakage,but the academic community has not reached a unified *** To find factors influencing the surveillance of re-emerging intracranial infections in elective neurosurgical *** Ninety-four patients who underwent elective craniotomy from January 1,2015 to December 31,2022 in the Department of Neurosurgery,First Hospital of Jilin University,were included in this *** those,45 patients were enrolled in the infection group,and 49 were enrolled in the control *** clinical data of the patients were collected and divided into three categories,including preoperative baseline conditions,intraoperative characteristics and postoperative infection *** data were analyzed using SPSS 26.0 *** There were 23 males and 22 females in the infection group with a mean age of 52.8±15.1 years and 17 males and 32 females in the control group with a mean age of 48.9±15.2 *** univariate analysis showed that the infection group had higher systolic blood pressures and postoperative temperatures,fewer patients who underwent a supratentorial craniotomy,more patients with a history of hypertension and higher initial postoperative white blood cell counts than the control group,with statistically significant differences(P<0.05).The multifactorial logistic regression analysis showed that a history of hypertension and a high postoperative body temperature were independent risk factors for postoperative infection in neurosurgical *** The results obtained in this study indicated that a history of hypertension and a high postoperative body temperature were independent risk factors for postoperative neurological symptoms.

关键词： Re-emerging infections Risk factors Neurosurgery Elective surgery Intracranial infections

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TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus

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Biomedical and Environmental Sciences 2018年第3期31卷 208-214页

作者： SHAO nan LI Fan NIE Kai FU Shi Hong ZHANG Wei Jia HE Ying LEI wen wen WANG Qian Ying LIANG Guo Dong CAO Yu xi WANG Huan Yu Department of Viral Encephalitis National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China State Key Laboratory of Infectious Disease Prevention and Control Beijing 102206 China State key laboratory for genetic engineering and molecular virology National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206China School of Public Health Shandong University Jinan 250012 Shandong China Office of laboratory management National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 102206 China

Objective To detect Japanese encephalitis virus（JEV） rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction（RT-PCR） detection system was *** By aligning the full-length sequences of JEV（G1-G5）, six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR *** With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/μL. The coefficients of variation of this real-time RT-PCR were all 〈 2.8%. The amplification efficiency of this method was between 90% and 103%.Conclusion A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.

关键词： Japanese encephalitis virus Genotype TaqMan real-time RT-PCR

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Origin and depositional model of deep-water lacustrine sandstone deposits in the 7th and 6th members of the Yanchang Formation(Late Triassic),Binchang area,Ordos Basin,China

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Petroleum Science 2017年第1期14卷 24-36页

作者： xi-xiang Liu xiao-Qi Ding Shao-nan Zhang Hao He State Key Laboratory of Oil and Gas Reservoir Geology and Exploitation Southwest Petroleum UniversityChengdu 610500SichuanChina School of Geoscience and Technology Southwest Petroleum UniversityChengdu 610500SichuanChina College of Energy Chengdu University of TechnologyChengdu 610059SichuanChina Second Production Plant Petro China Changqing Oilfield CompanyQinyang 745000GansuChina

Sandstones attributed to different lacustrine sediment gravity flows are present in the 7th and 6th members of the Yanchang Formation in the Ordos Basin, China. These differences in their origins led to different sandstone distributions which control the scale and connectivity of oil and gas reservoirs. Numerous cores and outcrops were analysed to understand the origins of these sandstones. The main origin of these sandstones was analysed by statistical methods, and well logging data were used to study their vertical and horizontal distributions. Results show that the sandstones in the study area accu- mulated via sandy debris flows, turbidity currents and slumping, and sandy debris flows predominate. The sand- stone associated with a single event is characteristically small in scale and exhibits poor lateral continuity. How- ever, as a result of multiple events that stacked gravity flow-related sandstones atop one another, sandstones are extensive overall, as illustrated in the cross section and isopach maps. Finally, a depositional model was developed in which sandy debris flows predominated and various other types of small-scale gravity flows occurred frequently, resulting in extensive deposition of sand bodies across a large area.

关键词： Sediment gravity flows - Sandy debris flows -Binchang area Yanchang Formation Ordos Basin

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Low-temperature dielectric relaxation associated with NbO_6 octahedron distortion in antimony modified potassium sodium niobate ceramics

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Journal of Materials Science & Technology 2022年第20期115卷 189-198页

作者： nan Zhang xiang Lv xi-xiang Zhang Jing Lyu Shuo-Wang Yang Jiagang Wu Department of Materials Science Sichuan UniversityChengdu 610065China Division of Physical Science and Engineering King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia Department of Chemistry National University of Singapore3 Science Drive 3117543Singapore Institute of High Performance Computing Agency for ScienceTechnology and Research1 Fusionopolis Way#16-16 Connexis138632Singapore

Although the antimony(Sb) has been widely used to modify potassium sodium niobate(KNN) ceramics for tailoring the phase structure and performance, the role of Sb still remains insufficiently understood,consequently hindering the understanding of the physical origin of high-performance KNN-based ceramics. Here, we combine the experiments and first-principles calculations to deeply reveal the effects of Sb on KNN ceramics. Our results reveal a re-entrant-like relaxation behavior near the rhombohedralorthorhombic(R-O) phase transition at the low content of Sb, which transforms into a canonical one at higher content of Sb. First-principles calculations show a significantly decreased difference in the bond length of six B-O bonds of Nb O6 octahedral in Sb-modified KNN ceramics compared to pristine KNN ceramics, responsible for the low-temperature re-entrant-like dielectric relaxation. Furthermore, the addition of Sb would soften the B-O repulsion and gradually break the long-range ferroelectric ordering,resulting in the occurrence of nanoscale domains and enhanced local heterogeneity. Finally, we find that the optimized piezoelectric properties are the trade-off between the long-range ferroelectric ordering and the local heterogeneity. Therefore, this work not only deeply reveals the effects of Sb on KNN ceramics from multi-scale perspectives but also helps the future composition design for achieving high piezoelectricity.

关键词： KNN-based ceramics Re-entrant-like relaxation Covalency Multiphase coexistence Local heterogeneity

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Interactive Effect of Grain Orientation and Grain Size on Magnetic Properties of Fe-78 wt% Ni Ribbons

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Journal of Materials Science & Technology 2012年第9期28卷 833-836页

作者： nan Wang Ruining Yang wenjing Yao Jinfeng Xu xixing wen Key Laboratory of Space Applied Physics and Chemistry Ministry of EducationSchool of ScienceNorthwestern Polytechnical UniversityXi'an 710072China School of Materials and Engineering Xi'an University of TechnologyXi'an 710048China

Fe-78 wt% Ni ribbons were prepared by the melt spinning technique and the interactive contribution of the grain size and grain orientation on the magnetic properties was examined. Heat treatment at 673 K for 1 h followed by furnace cooling was performed to show the annealing impact. At three wheel speeds of 10, 20, and 30 m/s, the saturation magnetization nearly does not change. High wheel speed and heat treatment are inclined to promote the growth of 〈001〉 grains. Although the 〈001〉 orientation is not the easy axis of magnetization, the improvement of the texture in this direction makes the coercivity decrease, which counteracts the inverse effect of the grain size at high wheel speed. It indicates that for preparing soft magnetic ribbons, the interactive contribution of grain orientation variation and the grain size should be considered.

关键词： Magnetic properties Rapid solidification Grain size Grain orientation

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Peutz-Jeghers syndrome with mesenteric fibromatosis: A case report and review of literature

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World Journal of Clinical Cases 2020年第3期8卷 577-586页

作者： Huai-Jie Cai Han Wang nan Cao Wei Wang xi-xi Sun Bin Huang The Fourth Clinical Medicine College Zhejiang Chinese Medical UniversityHangzhou 310053Zhejiang ProvinceChina Department of Ultrasound Zhejiang HospitalHangzhou 310013Zhejiang ProvinceChina

BACKGROUND Peutz-Jeghers syndrome(PJS) and mesenteric fibromatosis(MF) are rare diseases,and PJS accompanying MF has not been previously reported. Here, we report a case of a 36-year-old man with both PJS and MF, who underwent total colectomy and MF surgical excision without regular follow-up. Two years later, he sought treatment for recurrent acute abdominal pain. Emergency computed tomography showed multiple soft tissue masses in the abdominal and pelvic cavity, and adhesions in the small bowel and peritoneum. Partial intestinal resection and excision of the recurrent MF were performed to relieve the *** SUMMARY A 36-year-old male patient underwent total colectomy for PJS with MF. No regular reexamination was performed after the operation. Two years later, due to intestinal obstruction caused by MF enveloping part of the small intestine and peritoneum, the patient came to our hospital for treatment. Extensive recurrence was observed in the abdomen and pelvic cavity. The MF had invaded the small intestine and could not be relieved intraoperatively. Finally, partial bowel resection, proximal stoma, and intravenous nutrition were performed to maintain *** Regular detection is the primary way to prevent deterioration from PJS. Although MF is a benign tumor, it has characteristics of invasive growth and ready recurrence. Therefore, close follow-up of both the history of MF and gastrointestinal surgery are advisable. Early detection and early treatment are the main means of improving patient prognosis.

关键词： Peutz-Jeghers syndrome Mesenteric fibromatosis Recurrence Regular follow-up Case report

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Development and validation of a novel non-invasive test for diagnosing nonalcoholic fatty liver disease in Chinese children

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World Journal of Pediatrics 2024年第4期20卷 413-421页

作者： Zhe-wen Qin Qian-nan Ren Hong-xi Zhang Ya-Ru Liu Ke Huang Wei Wu Guan-Ping Dong Yan Ni Jun-Fen Fu Division of Endocrinology National Clinical Research Center for Child HealthNational Children’s Regional Medical CenterThe Children’s HospitalDepartment of EndocrinologyNational Clinical Research Center for Child HealthNational Children’s Regional Medical CenterThe Children’s HospitalZhejiang University School of MedicineHangzhou310052China

With the exploding prevalence of obesity,many children are at risk of developing nonalcoholic fatty liver *** anthropometric and laboratory parameters,our study aimed to develop a model to quantitatively evaluate liver fat content(LFC)in children with *** A well-characterized cohort of 181 children between 5 and 16 years of age were recruited to the study in the Endocrinology Department as the derivation *** external validation cohort comprised 77 *** assessment of liver fat content was performed using proton magnetic resonance *** and laboratory metrics were measured in all subjects.B-ultrasound examination was carried out in the external validation *** Kruskal-Wallis test,Spearman bivariate correlation analyses,univariable linear regressions and multivariable linear regression were used to build the optimal predictive *** The model was based on indicators including alanine aminotransferase,homeostasis model assessment of insulin resistance,triglycerides,waist circumference and Tanner *** adjusted R^(2)of the model was 0.589,which presented high sensitivity and specificity both in internal[sensitivity of 0.824,specificity of 0.900,area under curve(AUC)of 0.900 with a 95%confidence interval:0.783–1.000]and external validation(sensitivity of 0.918 and specificity of 0.821,AUC of 0.901 with a 95%confidence interval:0.818–0.984).Conclusions Our model based on five clinical indicators was simple,non-invasive,and inexpensive;it had high sensitivity and specificity in predicting LFC in ***,it may be useful for identifying children with obesity who are at risk for developing nonalcoholic fatty liver disease.

关键词： Biochemical markers Children Magnetic resonance spectroscopy Nonalcoholic fatty liver disease Pediatric obesity

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Compact 16-channel integrated charge-sensitive preamplifier module for silicon strip detectors

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Nuclear Science and Techniques 2020年第5期31卷 36-43页

作者： Dong-xi Wang Cheng-Jian Lin Lei Yang nan-Ru Ma Li-Jie Sun Feng Yang Hui-Ming Jia Fu-Peng Zhong Pei-Wei wen Department of Nuclear Physics China Institute of Atomic EnergyBeijing 102413China

In this study,a compact 16-channel integrated charge-sensitive preamplifier named the smart preamplifier(SPA)was developed to support the large-scale detector array used in modern nuclear physics *** types of SPA,namely SPA02 and SPA03(with external field effect transistor),have been manufactured to match silicon detectors with small and large capacitances,*** characteristics of the SPA include fast response of typically less than 6 ns for pulse rising time and low equivalent noise of 1.5 keV at zero input *** energy sensitivity and pulse decay time can be easily adjusted by changing the feedback capacitance Cfand resistance Rfin various applications.A good energy resolution of 24.4 keV for 5.803-MeV alpha particles from 244 Cm was achieved using a small-sized Si-PIN detector;for the silicon strip detectors in the test with the alpha source,a typical energy resolution of 0.6–0.8%was *** integrated SPA has been employed in several experiments of silicon strip detectors with hundreds of channels,and a good performance has been realized.

关键词： Silicon strip detector array 16-Channel integrated charge-sensitive preamplifier Preamplifier circuit design Preamplifier performance

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Self-Adaptive Control System for Additive Manufacturing Using Double Electrode Micro Plasma Arc Welding

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Chinese Journal of Mechanical Engineering 2021年第3期34卷 285-298页

作者： nan Li Ding Fan Jiankang Huang Shurong Yu wen Yuan Miaomiao Han School of Materials Science and Engineering Lanzhou University of TechnologyLanzhou 730050China State Key Laboratory of Advanced Processing and Recycling of Non-ferrous Metals Lanzhou University of TechnologyLanzhou 730050China School of Mechanical and Electrical Engineering Lanzhou University of TechnologyLanzhou 730050China

Wire arc additive manufacturing(WAAM)has been investigated to deposit large-scale metal parts due to its high deposition efficiency and low material ***,in the process of automatically manufacturing the high-quality metal parts by WAAM,several problems about the heat build-up,the deposit-path optimization,and the stability of the process parameters need to be well *** overcome these issues,a new WAAM method based on the double electrode micro plasma arc welding(DE-MPAW)was *** circuit principles of different metal-transfer models in the DE-MPAW deposition process were analyzed *** effects between the parameters,wire feed rate and torch stand-off distance,in the process of WAAM were investigated *** addition,a real-time DE-MPAW control system was developed to optimize and stabilize the deposition process by self-adaptively changing the wire feed rate and torch stand-off ***,a series of tests were performed to evaluate the control system’s *** results show that the capability against interferences in the process of WAAM has been enhanced by this self-adaptive adjustment ***,the deposition paths about the metal part’s layer heights in WAAM are ***,the appearance of the WAAM-deposited metal layers is also improved with the use of the control system.

关键词： Double electrode microplasma arc welding Additive manufacturing Wire feed rate Torch stand-off distance Self-adaptive adjustment

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