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Use of suppression subtractive hybridization strategy for cloning and identifying specifically expressed genes of renal cell carcinoma

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Chinese Science Bulletin 2001年第3期46卷 226-229页

作者： ZHANG Qiang ZHANG Zhiwen GONG Kan XIN Dianqi na yanqun GUO Yinlu Department of Urology and Institute of Urology The First Affiliated Hospital of Beijing Medical UniversityBeijing 100034China Department of Physiology Beijing Medical UniversityBeijing 100083China

Using suppression subtractive hybridization, a renal cell carcinoma (RCC) cDna subtractive library which only contains differently expressed cDnas between human RCC and normal kidney has been constructed. 200 clones were picked out randomly to perform enzyme digest analysis, a part of them underwent sequence analysis and Northern blot to identify RCC specially expressed genes. Results showed that 190 clones contain 50-400 bp inserts respectively. Sequence analysis was performed in 10 clones. All the 10 sequences were unknown before and derived from 6 unique novel genes among which the cDna insert RCC18 has five copies. Northern blot analysis showed that RCC18 cDna expressed highly in RCC, but there was no signal detected in normal kidney, and the full length of RCC18 was about 2.5 kb. The constructed cDna subtractive library of human RCC is a highly efficient one and lays the solid foundation for large-scale screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel

关键词： kidney neoplasms carcinoma suppression subtractive hybridization gene library.

Adjuvant hormone therapy after radical prostatectomy in highrisk localized and locally advanced prostate cancer: First multicenter, observational study in China

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Chinese Journal of Cancer Research 2019年第3期31卷 511-520页

作者： Dingwei Ye Wei Zhang Lulin Ma Chuanjun Du Liping Xie Yiran Huang Qiang Wei Zhangqun Ye yanqun na Department of Urology Fudan University Shanghai Cancer CenterShanghai 200032China Department of Urology Jiangsu Province Hospital Nanjing 210029China Department of Urology Peking University Third HospitalBeijing 100191China Department of Urology the Second Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310009China Department of Urology the First Affiliated Hospital of Zhejiang University School of MedicineHangzhou 310003China Department of Urology Renji Hospital Shanghai Jiaotong University School of MedicineShanghai 200127China Department of Urology West China HospitalSichuan UniversityChengdu 610041China Department of Urology Tongji HospitalTongji Medical College of Huazhong University of Science and TechnologyWuhan 430030China Department of Urology Peking University Shougang HospitalBeijing 100144China

Objective: Potential of combined androgen blockade(CAB) has not been explored extensively in Chinese males with prostate cancer(PCa). Therefore, this study evaluated the 2-year prostate-specific antigen(PSA) recurrence rate and quality of life(Qo L) in patients with high-risk localized and locally advanced PCa receiving adjuvant hormone therapy(HT) after radical prostatectomy(RP).Methods: This prospective, multicenter, observational study conducted in 18 centers across China enrolled patients with high-risk factor(preoperative PSA>20 ng/m L or Gleason score >7) or locally advanced PCa. Different adjuvant HT were administered after RP according to investigator’s decision in routine clinical *** of baseline and postoperative characteristics was assessed with recurrence rate. PSA recurrence rate and Functional Assessment of Cancer Therapy-Prostate(FACT-P) Qo L scores were recorded at 12 months and 24 months. Kaplan-Meier analysis was used to construct the PSA recurrence rate during ***: A total of 189 patients(mean age: 66.9±6.5 years) were recruited, among which 112(59.3%) patients showed serum PSA>20 ng/m L preoperatively. The highest postoperative pathological advancement noticed was from clinical T2(c T2) to pathological T3(p T3)(43.9%) stage. The majority of the patients(66.1%) received CAB as adjuvant HT, for a median duration of 20.0 months. The least recurrence(15.2%) was noticed in patients treated with CAB, followed by those treated with luteinizing hormone-releasing hormone agonist(LHRHa)(16.1%), and antiandrogen(19.0%), with non-significant difference noted among the groups. None of the baseline or postoperative characteristics was related with PSA recurrence in our study. The 24-month FACT-P Qo L score of119 patients treated for >12 months showed significant improvement above baseline compared with those treated for ≤12 ***: Adjuvant CAB therapy after RP showed reduction trend in 2-year PSA recurrence rate in

关键词： Adjuvant hormone therapy combined androgen blockade PSA recurrence quality of life radical prostatectomy

Cloning and functional research of renal cell carcinoma related novel gene——GYLZ-RCC18

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Chinese Science Bulletin 2001年第19期46卷 1622-1626页

作者： ZHANG Qiang CHEN Lin AI Junkui Chung Lee LIANG Lili TONG Ming ZHANG Zhiwen na yanqun QUO Yinglu Department of Urology and the Institute of Urology The First Affiliated HospitalPeking UniversityBeijing 100034China Fujian Haematology Institute The Union HospitalFuzhou 350001China Department of Physiology Peking UniversityBeijing 100083China Medical School of Northwestern University USA

After the renal cell carcinoma related novel gene fragment GYLZ-RCC18 was cloned by using suppression subtractive hybridization (SSH), we used the SMART RACE technology to clone the full length of GYLZ-RCC18 and performed chromosome location by the FISH method. RT-PCR was used to detect the expression of the first reading frame of GYLZ-RCC18 in different stages and grades of renal cell carcinoma tissue and other tissues. Also we trans-fected the antisense oligonucleotide of GYLZ-RCC18 to renal cell carcinoma cell line GRC-1, and analyzed the proliferation activity, growth speed, apoptosis and mortality changes in GRC-1. The results show that the full length of GYLZ-RCC18 (GenBank accession No.: BE825133) cDna is about 3.5 kb long which is located at No. 14 chromosome. GYLZ-RCC18 has a higher expression in higher grades and stages of renal cell carcinoma than in the lower ones. The expression of GYLZ-RCC18 in renal cell carcinoma was much higher than that in normal kidney and other tissues. After

关键词： kidney neoplasm carcinoma gene GYLZ-RCC18.

前列腺癌内分泌治疗中阻断肾上腺源性雄激素时机的实验研究

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中华泌尿外科杂志 2014年第8期35卷 625-628页

作者：孟帅那彦群北京大学首钢医院泌尿外科 100144

目的探究在前列腺癌的内分泌治疗中阻断肾上腺源性雄激素的恰当时机.方法 2013年2月至2014年3月,采用LNCaP细胞建立雄激素依赖的前列腺癌SCID鼠模型.首先设置未去势和去势两组,每组6只,于第1、4、7、10、14、17、21、28、35、42、49、5... 详细信息

目的探究在前列腺癌的内分泌治疗中阻断肾上腺源性雄激素的恰当时机.方法 2013年2月至2014年3月,采用LNCaP细胞建立雄激素依赖的前列腺癌SCID鼠模型.首先设置未去势和去势两组,每组6只,于第1、4、7、10、14、17、21、28、35、42、49、56天检测血清PSA并测量肿瘤体积,以确定去势后前列腺癌复发时间点;然后设置去势同时去除肾上腺组、去势后复发时间点去除肾上腺组以及去势后复发时间点假肾上腺切除术组3组,每组6只,按上述时间点检测血清PSA、测量肿瘤体积,并在第56天收获5组的肿瘤组织以检测组织内睾酮浓度.统计分析比较各组肿瘤进展情况的差异.结果去势组的复发时间为第14天,去势同时去除肾上腺组为第21天,去势后复发时间点去除肾上腺组为第35天.去势同时去除肾上腺组和去势后复发时间点去除肾上腺组肿瘤组织内睾酮浓度分别为（2.69±0.21）和（2.16±0.13） pmol/g,组间比较差异有统计学意义（P＜0.05）.结论相对于去势同时去除肾上腺疗法,去势后复发时间点去除肾上腺疗法的肿瘤进展较慢.

关键词：前列腺肿瘤肾上腺雄激素类内分泌治疗小鼠,SCID

Stromal cell can promote the proliferation of epithelial cell in prostate cancer

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Stromal cell can promote the proliferation of epithelial cel...

Squamous cell carcinoma in an enlarged prostatic utricle (one case report and literature review)

第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学术会议

作者： Guo Hongfeng and na yanqun Department of Urology, Peking University First Hospital, Institute of Urology, Peking University, Beijing 100034

Objective To find the effect of stromal cell on the proliferation of epithelial cell in prostatic carcinoma. Methods PC3 and PC3-AR cells were cocultured with immortal stromal cells. For experiments in vitro, we did g...

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Squamous cell carcinoma in an enlarged prostatic utricle (on...

第十三届全国泌尿外科学术会议暨第八届全球华人泌尿外科学术会议

作者： li xuesong, gong kan, xiao yunxiang,zhou liqun, he zhisong, li shuqing, na yanqun and guo yinglu Department of Urology, First Hospital of Peking University, Beijing ,China

A case of squamous cell carcinoma arising in the enlarged prostatic utricle was presented and related literatures were reviewed. Case report: A 39-year-old man was admitted to the First Hospital, Peking University, be... 详细信息

关键词： utricle müllerian ducts cysts squamous cell carcinoma adult

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大鼠良性增生前列腺腺上皮细胞系的建立

中华泌尿外科杂志 2014年第5期35卷 383-387页

作者：牛鹏飞蔡建良苑学礼那彦群北京大学首钢医院北京大学吴阶平泌尿外科医学中心 100144 北京世纪坛医院

目的探讨大鼠良性增生前列腺腺上皮细胞系的建立方法. 方法 2011年12月至2012年12月选取13周龄雄性自发性高血压大鼠20只,屏障环境下常规饲养至29周龄.取大鼠前列腺组织行苏木精-伊红染色鉴定前列腺上皮增生情况.取前列腺腹侧叶组织,... 详细信息

目的探讨大鼠良性增生前列腺腺上皮细胞系的建立方法. 方法 2011年12月至2012年12月选取13周龄雄性自发性高血压大鼠20只,屏障环境下常规饲养至29周龄.取大鼠前列腺组织行苏木精-伊红染色鉴定前列腺上皮增生情况.取前列腺腹侧叶组织,剪碎后用Ⅰ型胶原蛋白酶消化,收集细胞,分别使用WAJC-404、PrEGM培养液进行体外培养14 d并传代.免疫细胞化学方法（CK8/18）鉴定原代培养的增生前列腺腺上皮细胞的特异性表达.绘制细胞生长曲线,比较两种培养基培养前列腺腺上皮细胞的生长情况. 结果采用WAJC-404、PrEGM培养基体外原代培养自发性高血压大鼠前列腺腺上皮细胞均达14 d,并成功纯化和传代.免疫细胞化学实验证实原代培养的增生前列腺腺上皮细胞特异性表达细胞角蛋白CK8和CK18.细胞生长曲线显示：PrEGM培养基培养的腺上皮细胞与WAJC-404培养基相比,细胞形态更好,细胞活力更为旺盛,进入对数生长期的时间更短（4d与7d）,细胞生长曲线平均峰值更高（15.3× 104/ml与12.8× 104/nl）. 结论大鼠良性增生前列腺腺上皮细胞系可体外构建,PrEGM培养基比WAJC-404培养基更适合该细胞系的建立.

关键词：前列腺腺上皮细胞良性前列腺增生细胞系原代培养

基于混合现实引导的经皮肾穿刺肾脏模型的验证

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中华医学杂志 2018年第24期98卷 1962-1964页

作者：朱鹤李宁忱赵子臣王洪光张滨那彦群北京大学吴阶平泌尿外科医学中心北京大学首钢医院泌尿外科100144 北京大学吴阶平泌尿外科医学中心北京大学首钢医院放射科100144

目的应用实验用肾脏模型验证了一种新型基于混合现实技术的经皮肾穿刺方法，为经皮肾镜碎石取石术中经皮肾穿刺技术提供精准引导。方法6位泌尿外科医生对实验用肾脏模型进行了混合现实引导的经皮肾穿刺。统计每位医生计划穿刺的时间，... 详细信息

目的应用实验用肾脏模型验证了一种新型基于混合现实技术的经皮肾穿刺方法，为经皮肾镜碎石取石术中经皮肾穿刺技术提供精准引导。方法6位泌尿外科医生对实验用肾脏模型进行了混合现实引导的经皮肾穿刺。统计每位医生计划穿刺的时间，尝试穿刺的次数以及穿刺的时间。结果成功完成12次穿刺，中位计划时间和穿刺时间分别为13 （11～19）s和19 （15～44）s，所有医生进行1～2次穿刺即可确认穿刺成功。结论使用混合现实引导的经皮肾穿刺，有助于提高经皮肾穿刺的准确性，可以克服传统X线和超声技术的内在局限性，需要通过动物实验、临床试验来进一步验证。

关键词：肾结石混合现实经皮肾镜碎石取石术肾穿刺

全面打造双绿色医院建筑——北京大学吴阶平泌尿外科科研楼前期设计

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中国医院 2009年第5期13卷 78-80页

作者：韩智宏那彦群北京大学首钢医院北京市石景山区晋元庄路9号100144

从绿色建筑的理念出发,结合北京大学吴阶平泌尿外科科研楼的前期设计,针对我国具体情况,利用适宜技术,以设计控制为指导思想,从环境、能源、水资源、材料、室内环境质量等方面提高建筑的生态质量并有效地减少资源与环境负荷,使得建筑物... 详细信息

从绿色建筑的理念出发,结合北京大学吴阶平泌尿外科科研楼的前期设计,针对我国具体情况,利用适宜技术,以设计控制为指导思想,从环境、能源、水资源、材料、室内环境质量等方面提高建筑的生态质量并有效地减少资源与环境负荷,使得建筑物在建造和使用过程中节能、无污染,将打造"绿色医疗环境"的理念运用建筑设计之中,同时实现第二个绿色——使用上的人性化。

关键词：医院建筑节能人性化