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Silk-based nerve guidance conduits with macroscopic holes modulate the vascularization of regenerating rat sciatic nerve

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neural Regeneration Research 2025年第6期20卷 1789-1800页

作者： Carina Hromada Patrick Heimel Markus Kerbl lászlóGál Sylvia nürnberger Barbara Schaedl James Ferguson nicole Swiadek Xavier Monforte Johannes C.Heinzel Antal nógrádi Andreas H.Teuschl-Woller David Hercher Department life Science Engineering University of Applied Sciences Technikum WienViennaAustria Austrian Cluster for Tissue Regeneration ViennaAustria ludwig Boltzmann Institute for Traumatology The Research Center in Cooperation with AUVAViennaAustria University Clinic of Dentistry Medical University of ViennaViennaAustria Department of Plastic Reconstructive and Aesthetic SurgeryLandesklinikum Wiener Neustadt2700 Wiener NeustadtAustria Department of Anatomy Histology and EmbryologyAlbert Szent-Györgyi Medical SchoolUniversity of SzegedSzegedHungary Medical University of Vienna Department of Orthopedics and Trauma SurgeryDevision of Trauma SurgeryViennaAustria Department of Hand- PlasticReconstructive and Burn SurgeryBG Unfallklinik TuebingenUniversity of TuebingenTuebingenGermany

Peripheral nerve injuries induce a severe motor and sensory deficit. Since the availability of autologous nerve transplants for nerve repair is very limited, alternative treatment strategies are sought, including the use of tubular nerve guidance conduits(tnGCs). However, the use of tnGCs results in poor functional recovery and central necrosis of the regenerating tissue, which limits their application to short nerve lesion defects(typically shorter than 3 cm). Given the importance of vascularization in nerve regeneration, we hypothesized that enabling the growth of blood vessels from the surrounding tissue into the regenerating nerve within the tnGC would help eliminate necrotic processes and lead to improved regeneration. In this study, we reported the application of macroscopic holes into the tubular walls of silk-based tnGCs and compared the various features of these improved silk^(+) tnGCs with the tubes without holes(silk^(–) tnGCs) and autologous nerve transplants in an 8-mm sciatic nerve defect in rats. Using a combination of micro-computed tomography and histological analyses, we were able to prove that the use of silk^(+) tnGCs induced the growth of blood vessels from the adjacent tissue to the intraluminal neovascular formation. A significantly higher number of blood vessels in the silk^(+) group was found compared with autologous nerve transplants and silk^(–), accompanied by improved axon regeneration at the distal coaptation point compared with the silk^(–) tnGCs at 7 weeks postoperatively. In the 15-mm(critical size) sciatic nerve defect model, we again observed a distinct ingrowth of blood vessels through the tubular walls of silk^(+) tnGCs, but without improved functional recovery at 12 weeks postoperatively. Our data proves that macroporous tnGCs increase the vascular supply of regenerating nerves and facilitate improved axonal regeneration in a short-defect model but not in a critical-size defect model. This study suggests that further optimizatio

关键词： axon regeneration blood vessel functional recovery macroporous nerve lesion peripheral nerve repair sciatic nerve silk-based nerve guidance conduit vascularization

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Measurements of dihadron correlations relative to the event plane in Au+Au collisions at√^(S)nn=200 GeV

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Chinese Physics C 2021年第4期45卷 198-241页

作者： H.Agakishiev M.M.Aggarwal Z.Ahammed A.V.Alakhverdyants I.Alekseev J.Alford B.D.Anderson C.D.Anson D.Arkhipkin G.S.Averichev J.Balewski D.R.Beavis n.K.Behera R.Bellwied M.J.Betancourt R.R.Betts A.Bhasin A.K.Bhat H.Bichsel J.Bieleik J.Bielcikova B.Biritz l.C.Bland W.Borowski J.Bouchet E.Braidot A.V.Brandin A.Bridgeman S.G.Brovko E.Bruna S.Bueltmann I.Bunzarov T.P.Burton X.Z.Cai H.Caines M.Calderon de la Barca Sanchez D.Cebra R.Cendejas M.C.Cervantes Z.Chajecki P.Chaloupka S.Chattopadhyay H.F.Chen J.H.Chen J.Y.Chen l.Chen J.Cheng M.Cherney A.Chikanian K.E.Choi W.Christie P.Chung M.J.M.Codrington R.Corliss J.G.Cramer H.J.Crawford S.Dash A.Davila leyva l.C.De Silvat R.R.Debbe T.G.Dedovich A.A.Derevschikov R.Derradi de Souza l.Didenko P.Djawotho S.M.Dogra X.Dong J.l.Drachenberg J.E.Draper J.C.Dunlop l.G Efimov M.Elnim J.Engelage G Eppley M.Estienne l.Eun O.Evdokimov R.Fatemi J.Fedorisin A.Feng R.G.Fersch P.Filip E.Finch V.Fine Y.Fisyak C.A.Gagliardi D.R.Gangadharan A.Geromitsos F.Geurts P.Ghosh Y.n.Gorbunov A.Gordon O.Grebenyuk D.Grosnick S.M.Guertin A.Gupta W.Guryn B.Haag O.Hajkova A.Hamed l-X.Han J.W.Harris J.P.Hays-Wehle M.Heinz S.Heppelmann A.Hirsch E.Hjort G.W.Hoffmann D.J.Hofiman B.Huang H.Z.Huang T.J.Humanic l.Huo G.Igo P.Jacobs W.W.Jacobs C.Jena F.Jin J.Joseph E.G.Judd S.Kabana K.Kang J.Kapitan K.Kauder H.Ke D.Keane A.Kechechyan D.Kettler D.P.Kikola J.Kiryluk A.Kisiel V.Kizka A.G.Knospe D.D.Koetke T.Kollegger J.Konzer I.Koralt l.Koroleva W.Korsch l.Kotchenda V.Kouchpil P.Kravtsov K.Krueger M.Krus l.Kumar P.Kurnadi M.A.C.lamont J.M.landgraf S.laPointe J.lauret A.lebedev R.lednicky J.H.lee W.leight M.J.leVine C.lil l.li n.li W.li X.li X.li Y.li Z.M.li M.A.lisa F.liu H.liu J.liu T.ljubicic W.J.llope R.S.longacre W.A.love Y.lu E.V.lukashov X.luo G.l.Ma Y.G.Mai D.P.Mahapatra R.Majka O.I.Mall l.K.Mangotra R.Manweiler S.Margetis C.Markert H.Masui H.S.Matis Yu.A.Matulenko D.MeDonald T.S.McShane A.Meschanin R.Milner n.G.Minaev S.Mioduszewski A.Mischke M.K.Mitrovski B.Mohanty M.M.Mondal B.Morozov D.A.Morozov M.G.Munhoz M Argonne national laboratory Argonnellinois 60439USA Brookhaven national laboratory UptonNew York 11973USA University of Califomia BerkeleyCaliformnia 94720USA University of California DavisCalifornia 95616USA University of Califormnia Los AngelesCalifomia 90095USA Universidade Estadual de Campinas Sao PauloBrazil University of Illinois at Chicago ChicagoIlinois 60607USA Creighton University OmahaNebraska 68178USA Czech Technical University in Prague FNSPEPrague11519Czech Republic nuclear Physics Institute AS CR 25068 Rez/PragueCzech Republic University of Frankfurt FrankfurtGermany Institute of Physics Bhubaneswar 751005India Indian Institute of Technology MumbaiIndia Indiana University BloomingtonIndiana 47408USA Alikhanov Instiute for Theoretical and Experimental Physics MoscowRussia University of Jammu Jammu 180001India Joint Institute for nuclear Research Dubna141980Russia Kent State University KentOhio 44242USA University of Kentucky LexingtonKentucky40506-0055USA Institute of Modern Physics LanzhouChina lawrence Berkeley national laboratory BerkeleyCalifornia 94720USA Massachusetts Institute of Technology CambridgeMA 02139-4307USA Mx-Planck-Institut fir Physik MunichGermany Michigan State University East LansingMichigan 48824USA Moscow Engineering Physics Institute Moscow Russia nIKHEF and Utrecht University AmsterdamThe Netherlands Ohio State University ColumbusOhio 43210USA Old Dominion University NorfolkVA23529USA Panjab University Chandigarh 160014India PennsyIvania State University University ParkPennsylvania 16802USA Institute of High Energy Physics ProtvinoRussia Purdue University West LafayetteIndiana 47907USA Pusan national University PusanRepublic of Korea University of Rajasthan Jaipur 302004India Rice University HoustonTexas 77251USA Universidade de Sao Paulo Sao PauloBrazil University of Science&Technology of China Hefei 230026.China Shandong University JinanShandong 250100China Shanghai Institute of Applied Physics Shanghai 201800Chi

Dihadron azimuthal correlations containing a high transverse momentum(pr)trigger particle are sensit-ive to the properties of the nuclear medium created at RHIC through the strong interactions occurring between the traversing parton and the medium,*** measurements revealed a strong modification to di-hadron azimuthal correlations in Au+Au collisions with respect to ptp and d+Au *** modification in-creases with the collision centrality,suggesting a path-length or energy density dependence to the je-quenching *** paper reports STAR measurements of dihadron azimuthal correlations in mid-central(20%-60%)Au+Au collisions at√^(S)nn=200 GeV as a function of the trigger particle's azimuthal angle relative to the event plane,Ф_(s)=|Ф_(t)-ψ_(Ep)|.The azimuthal correlation is studied as a function of both the trigger and associated particle *** subtractions of the combinatorial background and anisotropic flow,assuming Zero Yield At Minimum(ZYAM),are *** correlation results are first discussed with subtraction of the even harmonic(elliptic and quadrangu-lar)flow *** away-side correlation is strongly modifed,and the modification varies withФ_(s),with a double-peak structure for out-of-plane trigger *** near-side ridge(long range pseudo-rapidity△_(η)correla-tion)appears to drop with increasingФ_(s)while the jet-like component remains approximately *** correla-tion functions are further studied with the subtraction of odd harmonic triangular flow background arising from *** is found that the triangular flow,while responsible for the majority of the amplitudes,is not sufficient to explain theφs-dependence of the ridge or the away-side double-peak *** dropping ridge withФ_(s)could be attributed to aФ_(s)-dependent lliptie anisotropy;however,the physics mechanism of the ridge remains an open *** with aФ_(s)-dependent elliptic flow,the away-side correlation structure

关键词： relativistic heavy ion collisions dihadron correlations jet-medium interactions anisotropic flow background event plane

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Amyloid-beta pathology-induced nanoscale synaptic disruption:the case of the GABA_B-GIRK assembly

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neural Regeneration Research 2025年第5期20卷 1409-1410页

作者： Rafael lujan Alejandro Martín-Belmonte Sergi Ferré Francisco Ciruela Synaptic Structure laboratory Instituto de Biomedicina de la UCLM(IB-UCLM)Departamento de Ciencias MédicasInstituto de Investigación en Salud de Castilla-La Mancha(IDISCAM)Facultad de MedicinaUniversidad Castilla-La ManchaCampus BiosanitarioC/AlmansaAlbaceteSpain Integrative neurobiology Section National Institute on Drug AbuseIntramural Research ProgramNational Institutes of HealthBaltimoreMDUSA Pharmacology Unit Department of Pathology and Experimental TherapeuticsSchool of Medicine and Health SciencesInstitute of NeurosciencesUniversity of BarcelonaNeuropharmacology&Pain GroupNeuroscience ProgramBellvitge Institute for Biomedical ResearchL’Hospitalet de LlobregatSpain

Alzheimer's disease (AD) is characterized by an imbalance between excitatory and inhibitory brain networks,leading to aberrant homeostatic synaptic *** has progressively been recognized as syna ptopathy and syna ptic dysfunction has been identified as a key component of its pathogenesis (Schirinzi et al.,2020).Syna ptic dysfunction is believed to precede synapse loss,a primary biological correlate of cognitive decline in AD,inevita bly associated with neuronal death.

关键词： Alzheimer pathology

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Dilatometric Sintering of Ti-2A1 and Ti-5AI Elemental Powders

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Journal of Materials Science & Technology 2007年第3期23卷 363-366页

作者： Bharat B.PAnIGRAHI n.subba REDDY Karabi DAS M.M.GODKHInDI Department of Metallurgical and Materials Engineering Indian Institute of Technology Kharagpur

The effect of Al addition （2 and 5 at. pct） on sintering kinetics of Ti power were investigated. Al reduces the sintering rates, sinter density, increases activation energy of sintering and accelerates the grain growth. Sintering was controlled by mixed mode, i.e. transient liquid phase sintering, formation of intermetallics, and Ti grain boundary diffusion in TiAl2 and other intermetallics.

关键词： Dilatometry Sintering Diffusion Activation energy

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PGE and isotopic characteristics of Shergol and Suru Valley Ophiolites,Western ladakh:Implications for supra-subduction tectonics along Indus Suture Zone

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Geoscience Frontiers 2021年第3期12卷 473-488页

作者： Irfan Maqbool Bhat Talat Ahmad D.V.subba Rao Srinivasan Balakrishnan n.V.Chalapathi Rao Department of Earth Sciences University of KashmirSrinagar 190006India Vice Chancellors Office University of KashmirSrinagar 190006India Geochemistry Division CSIR-National Geophysical Research Institute(NGRI)Hyderabad 500007India Department of Earth Sciences Pondicherry UniversityPuducherry 605014India Centre of Advanced Study in Geology Institute of ScienceBanaras Hindu UniversityVaranasi 221005India

Present study reports the PGE-geochemistry of mantle peridotites and nd-isotope geochemistry of arc related mafic rocks from the Indus Suture Zone(ISZ),western *** total PGE concentration of the Shergol and Suru Valley peridotites(∑PGE=96-180 ppb)is much higher than that of the primitive mantle and global ophiolitic mantle *** studied peridotites show concave upward PGE-distribution patterns with higher palladium-group PGE/Iridium-group PGE ratios(i.e.,0.8-2.9)suggesting that the partial melting is not the sole factor responsible for the evolution of these *** observed PGE-distribution patterns are distinct from residual/refractory mantle peridotites,which have concave downward or flat PGE-distribution *** enrichment of palladium-group PGE as well as other whole-rock incompatible elements(e.g.,lIlE and lREE)and higher Pd/Ir ratio(1.1-5.9)reflects that these peridotites have experienced fluid/melt interaction in a supra-subduction zone(SSZ)tectonic ***,the Shergol mafic intrusives and Dras mafic volcanics,associated with the studied peridotites,have high^(143)nd/^(144)nd ratios(i.e.,0.512908-0.513078 and 0.512901-0.512977,respectively)and positiveε_(nd)(t)(calculated for t=140 Ma)values(i.e.,+5.3 to+8.6 and+5.1 to+6.6,respectively),indicating derivation from depleted mantle sources within an intra-oceanic arc setting,similar to Spongtang and nidar ophiolites from other parts of ladakh *** transition from SSZ-type Shergol and Suru Valley peridotites to Early Cretaceous tholeiitic Shergol mafic intrusives followed by tholeiitic to calc-alkaline Dras mafic volcanics within the neo-Tethys Ocean exhibit characteristics of subduction initiation mechanism analogous to the Izu-Bonin-Mariana arc system within western Pacific.

关键词： PGE nd-isotope Mantle peridotites Ophiolites ladakh neo-Tethys Ocean

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Anti-amyloid antibodies in Alzheimer’s disease: what did clinical trials teach us?

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neural Regeneration Research 2025年第4期20卷 1092-1093页

作者： Danko Jeremic lydia Jiménez-Díaz Juan D.navarro-lópez neuroPhysiology&Behavior lab University of Castilla-La ManchaBiomedical Research Center(CRIB)School of Medicine of Ciudad RealCiudad RealSpain

Although many causes of Alzheimer’s disease(AD)may exist,both the original amyloid cascade and tau hypotheses posit that abnormal misfolding and accumulation of amyloid-β(Aβ)and tau protein is the central event causing the ***,that conclusion could be only partly true,and there is conflicting evidence about the role of both proteins in AD,being able to precede and influence one *** researchers argue that these proteins are mere executors rather than primary causes of ***,there have been continuing refinements of both hypotheses,with alternative explanations proposed.Aβand tau proteins may be independently involved in specific neurotoxic pathways;yet there may be other crucial processes going on in early ***,accumulating evidence suggests that Aβand tau act synergistically,rather than additively in disease onset(Jeremic et al.,2021,2023a).

关键词： amyloid Alzheimer additive

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Flotation studies on low grade graphite ore from eastern India

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International Journal of Mining Science and Technology 2015年第3期25卷 415-420页

作者： Vasumathi n. Vijaya Kumar T.V. Ratchambigai S. subba Rao S. Bhaskar Raju G. CSIR-national Metallurgical laboratory Madras Centre CSIR Madras Complex

A low grade graphite ore from eastern India was beneficiated by flotation to improve its quality. The ore was composed of 87.80%ash and 8.59%fixed carbon. Primary coarse wet grinding （d80：186 μm） followed by rougher flotation in Denver flotation cell using diesel as collector and pine oil as frother yielded a rougher concentrate. Regrinding （d80：144 μm） of this rougher concentrate was opted for further libera-tion of graphite. It was followed by cleaning in laboratory flotation column. This combined process of relatively coarse primary grinding followed by regrinding and cleaning in flotation column resulted in final concentrate of 7.44% yield with 89.65% fixed carbon and 6.00% ash. This approach of two-stage grinding to recover the flake graphite at the coarsest possible grind can help to minimize grinding energy costs. A conceptual flow sheet which is cost effective was developed based on this methodology.

关键词： Graphite liberation Froth flotation Column flotation Flow-sheet development

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Pro-resolving lipid mediator reduces amyloid-β42–induced gene expression in human monocyte–derived microglia

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neural Regeneration Research 2025年第3期20卷 873-886页

作者： Ying Wang Xiang Zhang Henrik Biverstål nicolas GBazan Shuai Tan nailin li Makiko Ohshima Marianne Schultzberg Xiaofei li Department of neurobiology Care Sciences&SocietyDivision of NeurogeriatricsKarolinska InstitutetStockholmSweden Department of neurology Neuroscience CenterThe First Hospital of Jilin UniversityChangchunJilin ProvinceChina Department of Physiology and Pharmacology Karolinska InstitutetStockholmSweden Department of Biosciences and nutrition Karolinska InstitutetHuddingeSweden neuroscience Center of Excellence Louisiana State UniversityNew OrleansLAUSA Department of Medicine SolnaClinical Pharmacology GroupKarolinska University HospitalStockholmSweden

Specialized pro-resolving lipid mediators including maresin 1 mediate resolution but the levels of these are reduced in Alzheimer's disease brain, suggesting that they constitute a novel target for the treatment of Alzheimer's disease to prevent/stop inflammation and combat disease pathology. Therefore, it is important to clarify whether they counteract the expression of genes and proteins induced by amyloid-β. With this objective, we analyzed the relevance of human monocyte–derived microglia for in vitro modeling of neuroinflammation and its resolution in the context of Alzheimer's disease and investigated the pro-resolving bioactivity of maresin 1 on amyloid-β42–induced Alzheimer's disease–like inflammation. Analysis of RnA-sequencing data and secreted proteins in supernatants from the monocyte-derived microglia showed that the monocyte-derived microglia resembled Alzheimer's disease–like neuroinflammation in human brain microglia after incubation with amyloid-β42. Maresin 1 restored homeostasis by down-regulating inflammatory pathway related gene expression induced by amyloid-β42 in monocyte-derived microglia, protection of maresin 1 against the effects of amyloid-β42 is mediated by a re-balancing of inflammatory transcriptional networks in which modulation of gene transcription in the nuclear factor-kappa B pathway plays a major part. We pinpointed molecular targets that are associated with both neuroinflammation in Alzheimer's disease and therapeutic targets by maresin 1. In conclusion, monocyte-derived microglia represent a relevant in vitro microglial model for studies on Alzheimer's disease-like inflammation and drug response for individual patients. Maresin 1 ameliorates amyloid-β42–induced changes in several genes of importance in Alzheimer's disease, highlighting its potential as a therapeutic target for Alzheimer's disease.

关键词： Alzheimer's disease amyloid-β maresin microglia monocyte neuroinflammation resolution RnA-sequencing specialized pro-resolving lipid mediator

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Synthesis,isolation,identification and characterization of new process-related impurity in isoproterenol hydrochloride by HPlC,lC/ESI-MS and nMR

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Journal of Pharmaceutical Analysis 2017年第6期7卷 394-400页

作者： neeraj Kumar subba Rao Devineni Prasad Reddy Gajjala Shailendra Kumar Dubey Pramod Kumar Chemical Research Department Micro Labs LtdAPI DivisionBommasandra-Jigani Link RoadKIADB INDL AreaBommasandraBangalore 560105KarnatakaIndia

One unknown impurity （Imp-II） during the analysis of laboratory batches of isoproterenol hydrochloride was detected in the level ranging from 0.04% to 0.12% by high performance liquid chromatography with UV detection. The unknown impurity structure was proposed as 4-[2-（propan-2oylamino）ethyl]benzene-l,2-diol （Imp-II） using the liquid chromatography-mass spectrophotometry （lC-MS） analysis. Imp-II was isolated by semi-preparative liquid chromatography from the impurity-enriched reaction crude sample. Its proposed structure was confirmed by nuclear magnetic spectroscopy such as 1H, 13C, DEPT （1D nMR）, HSQC （2D nMR） and infrared spectroscopy （IR）, and retention time and purity with HPlC followed by the chemical synthesis. Due to less removable nature of Imp-II during the purification, the synthetic process was optimized proficiently to control the formation of Imp-II below to the limit 〈 0.12% in the course of reaction. The new chemical route was developed for the preparation of this impurity in required quantity with purity to use as reference standard. The most probable mechanism for the formation of Imp-II was discussed in details.

关键词： Isoproterenol hydrochloride Impurities HPlC nMR

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Copper Salt of 12-Tungstophosphoric Acid： An Efficient and Reusable Heteropoly Acid for the Click Chemistry

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Chinese Journal of Chemistry 2013年第4期31卷 534-538页

作者： K. V. Purnima D. Sreenu n. Bhasker K. nagaiah n. lingaiah B. V. subba Reddy J. S. Yadav Division of Organic Chemistry Indian Institute of Chemical Technology Hyderabad 500 007 India Division oflnorganie and Physical Chemistry Indian Institute of Chemical Technology Hyderabad 500 007 India

Three components coupling of alkyl bromide, sodium azide and alkyne has been achieved using a catalytic amount of copper-exchanged phosphotungstic acid （Cu-TPA） in the presence of triethyl amine in DMF to afford substituted triazoles in good yields with high selectivity. Interestingly, the coupling of alkyl azide with alkyne pro- ceeds readily at room temperature to furnish 1,2,3-triazoles in excellent yields. The catalyst can be recovered and reused for three to four subsequent runs with a minimal decrease of activity. The use of copper modified heter- opolyacids makes this procedure simple, convenient and environmentally friendly.

关键词： Cu-exchanged heteropoly acids click reaction three-component reactions triazoles

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