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Pedosphere 2002年第4期12卷 329-337页

作者： n.k.howes S.E.SMITH ZHUYOnGGUAn DepartmentofSoilandWater TheUniversityofAdelaideGlenOsmondSA5064（Australia） ResearchCentreforEco－EnvironmentalSciences theChineseAcademyofSciencesBeijing100085（China） SouthAustraliaResearchandDevelopmentInstitute GlenOsmondSA5064（Australia）

A sand-based culture system using rock phosphate (P) was developed to simulate the situation in alkalinesoils, with respect to the dominant P form, and five wheat cultivars (Excalibur, Brookton, krichauff, Westoniaand Sunco) were tested in this screening system to compare their P uptake and utilisation efficiencies. Resultsshowed that these cultivars differed significantly in their ability to acquire P from the sparingly available form(rock phosphate in this case). The accumulation of P by Brookton was three times that by krichauff. Pconcentrations in plant tissues did not differ significantly, indicating that all cultivars were similar in Putilisation efficiency. A further experiment showed that the greater ability of a cultivar to take up P fromsparingly available form was related to the ability of a cultivar to acidify the rhizosphere. Seed P content wasa confounding factor in this system, and the use of relatively uniform seed with similar P content, preferablylow, was conducive to a successful outcome of the screening process.

关键词： phosphorus efficiency sand-culture uptake wheat genotypes

六倍体小麦中1BL/1RS染色体易位的快速测定

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国外农学（麦类作物） 1990年第2期 21-22页

作者： n.k.howes 周桂莲

目前,许多国家在冬、春性六倍体小麦育种中都在利用1BL(小麦)/1RS(黑麦)染色体易位系。具有这种易位系的小麦品种能够抗白粉、秆锈、叶锈、条锈等主要小麦病害,并且似乎还可以改进小麦的农艺性状。但是,含有1BL/1RS易位的小麦面粉的面... 详细信息

目前,许多国家在冬、春性六倍体小麦育种中都在利用1BL(小麦)/1RS(黑麦)染色体易位系。具有这种易位系的小麦品种能够抗白粉、秆锈、叶锈、条锈等主要小麦病害,并且似乎还可以改进小麦的农艺性状。但是,含有1BL/1RS易位的小麦面粉的面团不耐揉搓,而且粘性增大,这两个性状都对制做面包不利。因此,在小麦品质育种中识别这些性状是很有必要的。易位系面团粘性的增强归咎于水溶性蛋白质和醇溶谷蛋白含量的提高。此外,

关键词：小麦六倍体染色体易位快速测定

外周动脉疾病患者中铁储备降低与心血管结局:一项随机对照试验

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世界核心医学期刊文摘（心脏病学分册） 2007年第8期3卷 2-3页

作者： Zacharski L.R. Chow B.k. howes P.S. 刘少伟 Dr.Research Service(151) VA Medical Center 215 N Hartland Rd White River Junction VT 05001 United States

背景:超过生理需要的铁聚集可通过增强铁催化、自由基介导的氧化应激而导致心血管疾病风险增高。目的:在一组有症状性外周动脉疾病(PAD)患者中验证静脉切开降低机体铁储备可影响患者临床结局的假说。设计、机构和患者:基于铁(Fe)和动脉... 详细信息

背景:超过生理需要的铁聚集可通过增强铁催化、自由基介导的氧化应激而导致心血管疾病风险增高。目的:在一组有症状性外周动脉疾病(PAD)患者中验证静脉切开降低机体铁储备可影响患者临床结局的假说。设计、机构和患者:基于铁(Fe)和动脉粥样硬化研究(FeAST)(VA合作研究#410)进行一项多中心、随机、对照、单盲性临床试验,该试验于1999年5月1日至2005年4月30日实施,属于退伍军人事务部合作研究项目,共纳入1277例有症状但病情稳定的PAD患者。

关键词：外周动脉疾病铁储备随机对照试验心血管疾病机体铁单盲氧化应激非致死性静脉切开合作研究

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用分子分析法筛选高危人群中的胰腺导管腺癌

世界核心医学期刊文摘（胃肠病学分册） 2005年第10期1卷 30-30页

作者： McFaul C. howes n. W. Gre-enhalf 李宏宇 Division of Surgery and Oncology University of Liverpool UCD Building Daulby Street Liverpool L69 3GA United Kingdom Dr.

Background&Aims: Screening of high-risk groups for pancreatic cancer has not been adopted because of concerns regarding specificity and sensitivity. Suitability of a combination of 3 novel molecular screening techniques was investigated. Methods: Pancreatic juice was extracted from 146 patients with pancreatic ductal adenocarcinoma, chronic pancreatitis, or biliary tract stones. p53 mutations were analyzed by using a modified yeast functional assay,k-ras status was analyzed using mutation-specific real-time PCR and the proportion of p16Ink4a promoter methylation was estimated using comparative methylation-specific real-time PCR. Results: p53 mutations were detected in 20 of 48 (42%) cancer cases, none of 49 controls, and 2 of 49 (4%) patients with pancreatitis. k-ras mutations were detected in 31 of 57 (54%) cancer patients, 13 of 61 (21%) controls, and 23 of 67 (34%) patients with pancreatitis. Twenty-six of 42 (62%) cancer patients had promoter methylation levels > 12%, compared with 3 of 24 (13%) controls, and 2 of 26 (8%) with pancreatitis. Mutations in p53 or high-level p16Ink4a promoter methylation occurred in 29 of 36 (80%) patients with cancer, 3 of 24 (13%) controls, and 3 of 22 (13%) with pancreatitis. Three patients (8%) of 36 with cancer; 14 of 24 (58%) controls, and 13 of 22 (59%) patients with pancreatitis had no marker. The gallstone disease patients had a high rate of positive k-ras mutations, possibly reflecting the fact that they were not disease free. Conclusions: Combination molecular analysis increased the discrimination between patients with malignant and benign disease. This level of discrimination would allow patients in high-risk groups to be stratified from negligible risk to over 50%probability of an early cancer.

关键词：胰腺导管腺癌高危人群胰腺癌患者启动子甲基化胆石症患者早期肿瘤新型分子功能测定增高幅度特异性