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世界核心医学期刊文摘（儿科学分册） 2006年第4期2卷 39-39页

作者： Henriques-Coelho T. Oliva-Teles n. Fonseca-Silva M.L. J. Correia-Pinto 王一飞 Escola de Ciê ncias Da saú de Universidade Do Minho Campus de Gualtar 4709- 057 Braga Portugal

Tetrasomy of the short arm of chromosome 9 constitutes a rare condition resulting in a well clinically recognized syndrome. In our case, in addition to the characteristic phenotype at birth, the existence of a hernia-type Bochdalek diaphragmatic defect was found. Cytogenetic analysis revealed a nonmosaic case of an isochromosome of the entire short arm of chromosome 9 with no involvement of the heterochromatic region of the long arm: 47, XX, + i (9p). Because chromosome 9 contains several gene locus for enzymes and receptors of the retinoid pathway, this case potentially contributes to retinoid hypothesis in the etiology of congenital diaphragmatic hernia.

关键词：先天性膈疝细胞遗传学分析类视黄醇嵌合体短臂异染色质代谢途径基因位点病因学生时

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经腹阴道骶骨后壁固定术网片腐蚀的发生率和处理

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世界核心医学期刊文摘（妇产科学分册） 2005年第10期1卷 11-11页

作者： Begley J.S Kupferman S.P Kuznetsov D.D H.g. Muntz 张丽娟 Virginia mason Medical Center Mailstop X8- GYN 1100 Ninth Ave Seattle WA 98111 United States.Dr

Objective: This study was undertaken to evaluate the occurrence and management of mesh erosions in patients undergoing abdominal sacrocolpopexy. Study design: A retrospective chart review of the abdominal sacrocolpopexy procedure (n = 92)between 1997 and 2003 was performed. Patients with mesh erosion were identified. Incidence by graft type and treatment required for erosion resolution was analyzed with χ 2 and Fisher exact test. Results: Erosions occurred in 7.6 % (7/92). Erosions were identified only in patients with gore-Tex (3/33, 9% ) or silicone-coated mesh (4/21, 19% ) compared with none of 38 patients with polypropylene mesh (n = 24) or fascia (n = 14) grafts (P =. 068.). Partial excision of exposed graft resolved all 3 gore-Tex erosions, compared with none of the siliconecoated mesh erosions (P =. 03). Complete graft removal was required to resolve silicone-coated mesh erosions. Conclusion: we observed a high rate of erosion with gore-Tex and siliconecoated mesh. Partial graft excision was adequate for gore-Tex erosions, but complete graft removal was necessary to resolve erosions associated with silicone-coated mesh.

关键词：骶骨后壁移植片精确检验图表分析胶膜受经切除术研究设计腐蚀率

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Rapid EST isolation from chromosome 1R of rye

Rapid EST isolation from chromosome 1R of rye

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中国遗传学会第八次代表大会暨学术讨论会

作者： Ruo-nan Zhou~(1,4) Rui Shi~(1,5) Shu-Mei Jiang~(1,2) wei-Bo Yin~1 Huang-Huang wang~1 Yu-Hong Chen~1 Jun Hu~1 Richard RC wang~3 Xiang-Qi Zhang~1 Zan-Min Hu~1 1 Institute of genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,P.R.China 2 South China Sea Institute of Oceanology,Chinese Academy of Sciences,guangzhou 510301,P.R.China 3 USDA-ARS,FRRL,Utah State University,Logan,UT 84322-6300,Usa 4 graduate University of Chinese Academy of Sciences,Beijing 100049,P.R.China 5 Forest Biotechnoiogy group,n.C.State University,Campus Box 7247,Raleigh,nC 27695-7247,Usa

Background To obtain important expressed sequence tags(ESTs)located on specific chromosomes is currently *** of single-chromosome EST library could be an efficient strategy to isolate important ESTs located on specific *** this research we developed a method to rapidly isolate ESTs from chromosome 1R of rye by combining the techniques of chromosome microdissection with hybrid specific amplification(Hsa).Results Chromosome 1R was isolated by a glass needle and digested with proteinase K(PK).The DnA of chromosome 1R was amplified by two rounds of PCR using a degenerated oligonucleotide 6-Mw sequence with a sau3AI digestion site as the *** PCR product was digested with sau3AI and linked with adaptor Hsa1,then hybridized with the sau3AI digested cDnA with adaptor Hsa2 of rye leaves with and without salicylic acid(sa)treatment,*** hybridized DnA fragments were recovered by the Hsa method and cloned into pMD18-T *** cloned inserts were released by PCR using the partial sequences in Hsa1 and Hsa2 as the primers and then *** the 94 ESTs obtained and analyzed,6 were known sequences located on rye chromosome 1R or on homologous group 1 chromosomes of wheat;all of them were highly homologous with ESTs of wheat,barley and/or other plants in gramineae,some of which were induced by abiotic or biotic *** in this research were 22 ESTs with unknown functions, probably representing some new genes on rye chromosome 1R,Conclusion we developed a new method to rapidly clone chromosome-specific ESTs from chromosome 1R of *** information reported here should be useful for cloning and investigating the new genes found on chromosome 1R.

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