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Effect of age on quality of fresh and frozen-thawed semenin White Italian ganders

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Asian Journal of Andrology 2003年第2期5卷 89-93页

作者： E.Lukaszewicz W.Kruszynski n.fujihara Department of Poultry Breeding Department of Genetics and Animal Breeding Agricultural University of Wroclaw 51-630 Wroclaw PolandFukuoka Women's Junior College 6-16-4 Wakagidai Fukuma Fukuoka 811-3221 Japan

Aim: To comparatively evaluate the fresh semen quality of 1, 2 and 3-yr-old White Italian ganders (Anser anser L.) and the susceptibility of spermatozoa to freezing-thawing procedure. Methods: Semen was collected by dorso-abdominal massage every 2 days^3 days from three groups of ganders: 1-yr-old (n=11), 2-yr-old (n=7) and 3-yr-old (n=9). In the pooled fresh semen samples, the following parameters were evaluated: the ejaculate volume, the blood or fecal contamination and the motility, concentration and morphology of spermatozoa. Sperm motility and morphology were evaluated in the frozen-thawed semen. Semen diluted with EK extender was frozen in straws in a computerized freezing unit with 6 % dimethyl-formamide to -140 癈 at a rate 60 癈/min and then transferred into the Ln2 container. Straws with semen were thawed in a water bath at 60 癈. Results: The ejaculate volume decreased with the age (0.21 mL for 1-yr-old, 0.18 mL for 2-yr-old and 0.14 mL for 3-yr-old ganders); the sperm concentration increased with the age (327xl06mL-1 for 1-yr-old, 431xl06mL-1 for 2-yr-old and 547xl06mL-1 for 3-yr-old ganders); the number of live - normal sperm was significantly (Pn the 1-yr-old than that in the 2- and 3-yr-old ganders (26.61 %, 41.54 %and 35.9 %, respectively). The percentage of normal cells survived the freezing-thawing process was 37.7 %, 43.3 % and 40.9 % for 1-, 2- and 3-yr-old ganders, respectively. Conclusion: Freezing and thawing processes more significantly (Pnd morphology of spermatozoa in semen of 1-yr-old ganders in comparison with older males.

关键词： poultry gander age semen sperm morphology freezing

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公禽的副生殖器和副生殖液(上)

国外畜牧科技 1992年第4期19卷 21-23页

作者： n.fujihara 周虚

前言雄性哺乳动物射精过程中,精子与几种副性器官分泌的液体相遇。在精液排出的同时,这些液体进入精浆,成为精液的自然成分。雄性哺乳动物有几个代表性的副生殖器官,例如:输精管、壶腹、前列腺、精囊腺、尿道球腺(或Cowper氏腺),以及尿... 详细信息

前言雄性哺乳动物射精过程中,精子与几种副性器官分泌的液体相遇。在精液排出的同时,这些液体进入精浆,成为精液的自然成分。雄性哺乳动物有几个代表性的副生殖器官,例如:输精管、壶腹、前列腺、精囊腺、尿道球腺(或Cowper氏腺),以及尿道腺(或Littre氏腺)。

关键词：禽公禽副生殖器副生殖液

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公禽的副生殖器和副生殖液(下)

国外畜牧科技 1992年第5期19卷 15-18页

作者： n.fujihara 周虚

产生淋巴样液或泡沫的组织和器官不同种类的雄禽,其淋巴样液及泡沫是由不同组织或器官产生并排出的。这与哺乳动物副性器官不一样。泄殖腔旁脉管体(PVB) 已清楚,使公鸡、鸭、火鸡、珍珠鸡和鹌鹑阴茎勃起的泄殖腔旁脉管体(PVB)

关键词：家禽公禽副生殖器副生殖液

Proliferation of exogenously injected primordial germ cells (PGCs) into busulfan-treated chicken embryos

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Asian Journal of Andrology 1999年第4期1卷 187-190页

作者： H. Furuta n. fujihara Animal Resource Science Section Division of Bioresource and Bioenvironmental Sciences Graduate School Kyushu University Fukuoka 812-8581 JapanAnimal Resource Science Section Division of Bioresource and Bioenvironmental Sciences Graduate School Kyushu University Fukuoka 812-8581 Japan

Aim: This study was designed to investigate the effect of busulfan treatment on the proliferation of chicken primordialgerm cells (PGCs) in vivo, focusing on the preferential settlement of PGCs onto the germinal ridges of chicken em-bryos. Methods: Busulfan (250 ng/egg) was injected into the egg white of freshly oviposited fertilized eggs, whichwere then incubated. Embryonic development and viability were examined, and exogenous PGCs collected from embry-onic blood vessels were injected into the germinal crescent region of recipient embryos. The number of PGCs residedonto germinal ridges of the right and left sides were compared. Results: Busulfan had a slight harmful effect on theembryo viability and the PGCs proliferation. The number of PGCs resided onto the left side of germinal ridges wasslightly higher as compared with the right side. Conclusion: Busulfan suppressed the viability of embryos and the pro-liferation of endogenous PGCs in the recipient embryos. However, the number of exogenous PGCs proliferated washigher in embryos treated with busulfan than those without busulfan. Data also suggest the possibility of a preferentialresidence of PGCs toward the left side of the germinal crescent region as compared with the fight, which may be due toa more advanced functional development of the left gonad than the right. (Asian J Androl 1999 Dec; 1: 187-190)

关键词： chick embryo germ cells proliferation busulfan

Detection accuracy for epithelial dysplasia using an objective autofluorescence visualization method based on the luminance ratio

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International Journal of Oral Science 2017年第4期9卷 200-209页

作者： nanami yamamoto koji kawaguchi hisako fujihara mitsuhiko hasebe yuta kishi masaaki yasukawa kenichi kumagai yoshiki hamada department of oral and maxillofacial surgery school of dental medicinetsurumi universityYokohamaJapan department of oral hygiene tsurumi junior collegeYokohamaJapan

The autofluorescence visualization method （AVM） uses blue excitation light to assist in the diagnosis of epithelial dysplasia. It detects epithelial dysplasia as a black area, which is known as fluorescence visualization loss （FVL）. In this study, we evaluated the detection accuracy for epithelial dysplasia of the tongue using the objective AVM and assessed its possible clinical utility. Seventy-nine tongue specimens clinically suspected to have leukoplakia or squamous cell carcinoma （SCC） were analyzed. First, the AVM was subjectively performed using the Visually Enhanced Lesion scope （VELscope）, and the iodine-staining method was then performed. After biopsy, the histopathological results and the luminance ratio between the lesion and healthy tissue were compared, and a receiver operating characteristic curve was created. The cutoff value for the objective AVM was determined; the lesion was considered FVL-positive or -negative when the luminance ratio was higher or lower than the cutoff value, respectively. The histopathological diagnoses among the 79 specimens were SCC （n = 30）, leukoplakia with dysplasia （n = 34）, and leukoplakia without dysplasia （n= 15）. The cutoff value of the luminance ratio was 1.62, resulting in 66 FVL-positive and 13 FVL-negative specimens. The luminance ratio was significantly higher in the epithelial dysplasia-positive than -negative group （P〈O.O00 1）. The objective AVM showed much higher consistency between histopathological results than did the two methods （kappa statistic = 0.656）. In conclusion, objective autofluorescence visualization has a potential as an auxiliary method for diagnosis of epithelial dysplasia.

关键词： autofluorescence epithelial dysplasia oral cancer Visually Enhanced Lesion scope

Efficacy and safety of over-the-scope clip: Including complications after endoscopic submucosal dissection

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World Journal of Gastroenterology 2013年第18期19卷 2752-2760页

作者： noriko nishiyama Hirohito Mori Hideki Kobara Kazi Rafiq Shintarou fujihara Mitsuyoshi Kobayashi Makoto Oryu Tsutomu Masaki Department of Gastroenterology and neurology Faculty of Medicine Kagawa University Kagawa 761-0796 Japan Department of Pharmacology Faculty of Medicine Kagawa University Kagawa 761-0796 Japan

AIM: To retrospectively review the results of over-thescope clip (OTSC) use in our hospital and to examine the feasibility of using the OTSC to treat perforations after endoscopic submucosal dissection (ESD). METHODS: We enrolled 23 patients who presented with gastrointestinal (GI) bleeding, fistulae and perforations and were treated with OTSCs (Ovesco Endoscopy GmbH, Tuebingen, Germany) between november 2011 and September 2012. Maximum lesion size was defined as lesion diameter. The number of OTSCs to be used per patient was not decided until the lesion was completely closed. We used a twin grasper (Ovesco Endoscopy GmbH, Tuebingen, Germany) as a grasping device for all the patients. A 9 mm OTSC was chosen for use in the esophagus and colon, and a 10 mm device was used for the stomach, duodenum and rectum. The overall success rate and complications were evaluated, with a particular emphasis on patients who had undergone ESD due to adenocarcinoma. In technical successful cases we included not only complete closing by using OTSCs, but also partial closing where complete closure with OTSCs is almost difficult. In overall clinical successful cases we included only complete closing by using only OTSCs perfectly. All the OTSCs were placed by 2 experienced endoscopists. The sites closed after ESD included not only the perforation site but also all defective ulcers ***: A total of 23 patients [mean age 77 years (range 64-98 years)] underwent OTSC placement during the study period. The indications for OTSC placement were GI bleeding (n = 9), perforation (n = 10), fistula (n = 4) and the prevention of post-ESD duodenal artificial ulcer perforation (n = 1). One patient had a perforation caused by a glycerin enema, after which a fistula formed. Lesion closure using the OTSC alone was successful in 19 out of 23 patients, and overall success rate was 82.6%. A large lesion size (greater than 20 mm) and a delayed diagnosis (more than 1 wk) were the major contributing fac

关键词： Over-the-scope clip Gastrointestinal bleed-ing Endoscopic submucosal dissection complications Gastrointestinal fistulae Gastrointestinal perforation