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Eugenol targeting CrtM inhibits the biosynthesis of staphyloxanthin in Staphylococcus aureus

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Food Science and Human Wellness 2024年第3期13卷 1368-1377页

作者： Jiang Chang Bo Chen Zeqian Du Bowen Zhao Jiahui Li Ziyi Li kannappan Arunachalam Ting Shi Dongqing Wei Chunlei Shi MOST-USDA Joint Research Center for Food Safety School of Agriculture and BiologyState Key Laboratory of Microbial MetabolismShanghai Jiao Tong UniversityShanghai 200240China School of Life Sciences and Biotechnology Shanghai Jiao Tong UniversityShanghai 200240China

Staphylococcus aureus is a serious foodborne pathogen threatening food safety and public *** the emergence of methicillin-resistant Staphylococcus aureus(MRSA)increased the difficulty of *** *** is a crucial virulence factor of *** staphyloxanthin production could help the host immune system counteract the invading *** *** this study,we first screened for staphyloxanthin inhibitors using a virtual screening *** outcome of the virtual screening method resulted in the identification of eugenol(300μg/mL),which significantly inhibits the staphyloxanthin production in *** ATCC 29213,*** newman,MRSA ATCC 43300 and MRSA ATCC BAA1717by 84.2%,63.5%,68.1%,and 79.5%,*** outcome of the growth curve assay,field-emission scanning electron,and confocal laser scanning microscopy analyses confirmed that eugenol at the test concentration did not affect the morphology and growth of ***,the survival rate of *** ATCC 29213 and MRSA ATCC 43300 under H_(2)O_(2) pressure decreased to 51.9%and 45.5%in the presence of eugenol,*** quantitative RT-PCR and molecular simulation studies revealed that eugenol targets staphyloxanthin biosynthesis by downregulating the transcription of the crtM gene and inhibiting the activity of the CrtM *** together,we first determined that eugenol was a prominent compound for staphyloxanthin inhibitor to combat *** especially MRSA infections.

关键词： Staphylococcus aureus Staphyloxanthin Eugenol Virtual screening 4 4’-Diapophytoene synthase(CrtM)

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Predicting carbon nanotube forest attributes and mechanical properties using simulated images and deep learning

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npj Computational Materials 2021年第1期7卷 1214-1224页

作者： Taher Hajilounezhad Rina Bao kannappan Palaniappan Filiz Bunyak Prasad Calyam Matthew R.Maschmann Mechanical&Aerospace Engineering University of MissouriColumbiaMOUSA Electrical Engineering and Computer Science University of MissouriColumbiaMOUSA

Understanding and controlling the self-assembly of vertically oriented carbon nanotube(CnT)forests is essential for realizing their potential in myriad *** governing process–structure–property mechanisms are poorly understood,and the processing parameter space is far too vast to exhaustively explore *** overcome these limitations by using a physics-based simulation as a high-throughput virtual laboratory and image-based machine learning to relate CnT forest synthesis attributes to their mechanical *** CnTnet,our image-based deep learning classifier module trained with synthetic imagery,combinations of CnT diameter,density,and population growth rate classes were labeled with an accuracy of>91%.The CnTnet regression module predicted CnT forest stiffness and buckling load properties with a lower root-mean-square error than that of a regression predictor based on CnT physical *** results demonstrate that image-based machine learning trained using only simulated imagery can distinguish subtle CnT forest morphological features to predict physical material properties with high *** paves the way to incorporate scanning electron microscope imagery for high-throughput material discovery.

关键词： carbon properties overcome

A solar reactor for bio-diesel production from Pongamia oil: Studies on transesterfication process parameters and energy efficiency

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Chinese Journal of Chemical Engineering 2021年第12期34卷 218-224页

作者： Subbaiyan naveen kannappan Panchamoorthy Gopinath Rajagopal Malolan Ramesh Sai Jayaraman Krishnan Aakriti Jayaseelan Arun Department of Chemical Engineering SSN College of EngineeringKalavakkam 603110India Centre for Waste Management International Research CentreSathyabama Institute of Science and TechnologyJeppiaar Nagar(OMR)Chennai 600119India

Over exploitation of non-renewable energy reserves will lead to increase in price of petroleum *** there is a need for suitable and sustainable substitutes(renewable resource) for conventional *** this study,an efficient and environmental friendly method for production of bio-diesel from Pongamia(Karanja) oil has been developed using a solar *** the experimental study,the maximum temperature attained by the pongamia oil during the transesterification process was 64.1℃.The transesterification reaction was studied by varying different parameters such as reactant flow rate(5-20 L·h^(-1)),stirring speed(150-450 r·min^(-1)),catalyst mass loading(0.5%-2%) and methanol to oil ratio(3:1 to15:1).The maximum biodiesel yield was 83.11% at a flow rate of 5 L·h^(-1),stirring speed of 350 r·min^(-1),a methanol to oil ratio of 15:1,catalyst mass loading of 1% and reaction time of 270 *** physical and chemical properties of biodiesel was analyzed as per American Society for Testing Materials(ASTM) standards and it had density of 938 kg·m^(-3),viscosity(28.7×10^(-6) m~2·s^(-1)),acid value(9.45 mg KOH·(g oil)^(-1))and flash point(215℃).The energy efficiency of solar heating process was determined by comparing the net energy ratio of direct heating process and solar heating *** solar heating the net energy ratio(nER) was found to be 31.85 against 5.73 for direct ***,net energy efficiency index was calculated for 10 kg production scale and was found to be increasing when scaled up which means that the solar heating process is more effective even in scaled up production.

关键词： Biodiesel Biofuel Solar energy Optimization Pongamia oil Solar reactor

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Free radical scavenging potential of Lagenaria siceraria(Molina) Standl fruits extract

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Asian Pacific Journal of Tropical Medicine 2013年第1期6卷 20-26页

作者： Vijayakumar Mayakrishnan Selvi Veluswamy Krishnakumari Shanmuga Sundaram Priya kannappan noorlidah Abdullah Institute of Biological Science Faculty of ScienceUniversity of MalayaKuala Lumpur-50603Malaysia Department of Biochemistry Kongunadu Arts and ScienceCoimbatore-641029TamilnaduIndia Department of Biochemistry.PSG college of Arts and Science Coimbatore-641029TamilnaduIndia

Objective:To elucidate free radical scavenging activity of ethanolic extract Lagenaria siceraria （***）（Molina） ***:The free radical scavenging activity of the L siceraria （Molina） fruit extract was assayed by usingα,α-diphenyl-β-picrylhydrazyl（DPPH）. 2,20-azinobis 3-ethyl henzothiazoline-6-sulfonate（ABTS）,FRAP,reducing power,chelating ability andβ-carotene bleaching ***:The IC50values of DPPH and ABTS radicalscavenging activity was found to be 1.95 mg/mL and 19 mg/mL,*** ferrous chelation assay,the percentage of inhibition was found to be 89.21%.The reducing power of ethanolic extract of L siceraria（Molina） fruit was 0.068 at 1 mg/mL and increased to 0.192 at 5 mg/mL Theβ-carotene linoleate bleaching assay was 46.7%at 5 mg/mL and antioxidant activity using FRAP at 0.305 for 1 mg/mL to 0.969 for 5 mg/mL Conclusions:The results indicate that L siceraria （Molina） fruit could be an important sources of natural radical scavengers.

关键词： Lagenaria siceraria Antioxidant DPPH ABTS Free radical

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确定离心压缩机的管线载荷

石油化工设备技术 1982年第6期 71-75页

作者： S.kannappan 董长善北京石油设计院

确定离心压缩机管嘴上允许的管线载荷的导则,在 API 617—1979年版中已作了修改。本文是对这些修改的说明。很多人还在使用 API 617—1973年版的导则,得到的是不正确的允许载荷值。

关键词：离心压缩机导则分量进口出口管管线 API 组合力配合力 nEMA 方程式方程载荷值咀子当量直径

余弦—正弦函数方程在群上的一个推广(研究简报)

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深圳大学学报(理工版) 1985年第4期 86-88页

作者：钟巨康 P L.kannappan C.T.ng 深圳大学应用数学系加拿大Waterloo大学加拿大Waterloo大学

本文得到函数方程f(xy)=f(x)g(y)+g(x)f(y)十h(x)h(y)的一般解,其中f,g和h是定义在一个群上的复函数。

关键词：方程一般解任意函数正弦函数

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混纺超细纤维针织物的导湿性

国际纺织导报 2011年第9期39卷 38-40页

作者： B.G.Manohari J.kannappan P.Kandhavadivu T.Ramachandran 刘晨 Kumaraguru科技大学 PSG技术学院

水气在混纺织物中的流动是一种复杂的现象。服装应该有良好的吸湿性及导湿性,这样才能具有热湿舒适性。众所周知,亲水性高的材料吸湿性好。选用超细的聚酯、天丝纤维混纺的织物,探讨了其如何转移水分;同时选用100%的超细聚酯织物和100%... 详细信息

水气在混纺织物中的流动是一种复杂的现象。服装应该有良好的吸湿性及导湿性,这样才能具有热湿舒适性。众所周知,亲水性高的材料吸湿性好。选用超细的聚酯、天丝纤维混纺的织物,探讨了其如何转移水分;同时选用100%的超细聚酯织物和100%的超细天丝织物做有效性对比。测试并汇报了织物中水蒸气、液态水的转移特性,水分的扩散性及干燥能力。

关键词：超细聚酯纤维超细天丝纤维混纺针织物导湿性

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A solar reactor for bio-diesel production from Pongamia oil: Studies on transesterfication process parameters and energy efficiency

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