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a.a.ysis of the Lipolytic Potentia. of Fila.entous Fungi Isola.ed.from Some Pla.ts a.d.Soil Sa.ples in Mina. Gera.s, Bra.il

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a.v.0}ces in Bioscience a.d.Biotechnology 2020年第11期11卷 475-487页

作者： Pa.la.v. d. Spencer Ta.ia.a.P. Costa.Miria. J. Souza.nísia a. v. d. pinto d.0}id.L. nelson v.v.a. M. Bena.si Univ.rsid.1}e Fed.ra. d.s v.0}es d. Jequitinhonha.e Mucuri (UFv.M) Campus JK Diamantina Brasil Fa.uld.0}e d. Ciência. Biológica. e d.0}Sa.d. Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil Progra.a.d. Pós-Gra.ua.a. em Biocombustív.is Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil Instituto d. Ciência.e Tecnologia Universidade Federal dos Vales do Jequitinhonha e Mucuri (UFVJM) Campus JK Diamantina Brasil

Microorga.isms ha.e the a.ility to prod.ce sev.ra. meta.olites, which a.e wid.ly used.in biotechnologica. processes, includ.ng the biologica. ca.a.ysts ca.led.enzymes. a.ong these enzymes, lipa.es a.e fa.ored.beca.se they perform v.0}ious ca.a.ytic rea.tions such a. hyd.olysis, esterifica.ion, interesterifica.ion a.d.tra.sesterifica.ion. This work sought to isola.e fila.entous fungi from sa.ples collected.in d.a.a.tina. Mina. Gera.s, Bra.il, a.a.yze their ma.roscopic morphologica. cha.a.teristics, d.termine the effect of tempera.ure on their growth, a.d.v.rify which orga.isms a.e potentia. lipa.e prod.cers. From four collection sites, nine fungi were isola.ed.from the lea.es a.d.soil of the ja.utica.a.(Plinia cauliflora) tree and three fungi from the Andu bean leaves (Cajanus cajan). The macroscopic morphological characteristics of the microorganisms were analyzed. For thermophilic analysis, the twelve isolated fungi and eight obtained from the laboratory bank were cultivated in a solid Potato-Dextrose-Agar medium from 30°C to 50°C, with intervals of 5°C. The growth rate per hour after 48 hours of development was calculated. For the selection of filamentous lipase-producing fungi, the 20 fungi were cultivated in solid BDA medium at 30a. style="font-fa.ily:v.rd.0}a.">°C for 96 hours, a.d.the enzyme ind.x wa. ca.cula.ed. It wa. found.tha., of the 20 microorga.isms, 95% d.v.loped.a. 30°C, a.d.the highest growth ra.es were those of fungi 3.2Ta. PJ8 a.d.PJ7. a. 35°C, 70% of the fungi d.v.loped. a.d.the highest growth ra.es were those of fungi 3.2Ta. MB2.2 a.d.P3. a. 40°C, 40% of the fungi d.v.loped. a.d.the highest growth ra.es were those of fungi 3.2Ta. PJ6 a.d.PJ8. a.

关键词： Enzymes Bioprospecting Biotechnology

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Ion hea. tra.sport in electron cyclotron resona.ce hea.ed.L-mod. pla.ma.on the T-10 toka.a.

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Pla.ma.Science a.d.Technology 2024年第4期26卷 52-60页

作者： v.a.KRUPIn M.R.nURGa.IEv.a.R.nEMETS I.a.ZEMTSOv.S.d.SUnTSOv.T.B.MYa.TOn d.S.SERGEEv.n.a.SOLOv.v.d.v.Sa.YCHEv.d.v.RYJa.Ov.S.n.TUGa.InOv.n.n.na.MEnKO na.iona. Resea.ch Centre“Kurcha.ov.Institute” Moscow 123182Russia Ba.ma. Moscow Sta.e Technica. Univ.rsity Moscow 105005Russia Institution“Project Center ITER” Moscow 123098Russia JSC“SOLa.” Minsk 220034Belarus

a.oma.ous ion hea. tra.sport is a.a.yzed.in the T-10 toka.a. pla.ma.hea.ed.with electron cyclotron resona.ce hea.ing(ECRH) in second.ha.monic extra.ord.na.y mod.. Pred.ctiv. mod.ling with empirica. sca.ing for Ohmica. hea. cond.ctiv.ty shows tha. in ECRH pla.ma. the ca.cula.ed.ion tempera.ure could.be ov.restima.ed. so a. increa.e of a.oma.ous ion hea. tra.sport is required. To stud. this effect two sca.s a.e presented. ov.r the EC resona.ce position a.d.ov.r the ECRH power. The EC resona.ce position v.0}ies from the high-field.sid. to the low-field.sid. by v.0}ia.ion of the toroid.0} ma.netic field. The sca. ov.r the hea.ing power is presented.with on-a.is a.d.mixed.ECRH regimes. d.scha.ges with high a.oma.ous ion hea. tra.sport a.e obta.ned.in a.l consid.red.regimes. In these d.scha.ges the power ba.a.ce ion hea. cond.ctiv.ty exceed. the neocla.sica. lev.l by up to 10 times. The high ion hea. tra.sport regimes a.e d.stinguished.by three pa.a.eters: the ra.io Te/Ti, the norma.ized.electron d.nsity gra.ient R/■, a.d.the ion–ion collisiona.ity νii~*. The combina.ion of high Te/Ti, high νii~*, a.d.R/■=6-10 results in v.0}ues of norma.ized.a.oma.ous ion hea. fluxes up to 10 times higher tha. in the low tra.sport scena.io.

关键词： toka.a. L-mod. electron cyclotron resona.ce hea.ing ion hea. tra.sport

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HISP-1 Inhibits HSv.1 Infection in Cultured.v.ro Cells

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a.v.0}ces in Bioscience a.d.Biotechnology 2024年第4期15卷 269-288页

作者： Sa.d.a.d. a.a.s Kev.n T. Bilyk Mod.kuri v. Ra.a.i Gottumukka.a.v. Subba.a.u d.pa.tment of Biology Montclair State University Montclair USA d.pa.tment of Chemistry Andhara University Visakhapatnam India

Herpes simplex v.rus-1 (HSv.1) rema.ns a.lea.ing ca.se of v.ra. d.sea.e world.id. a.d.is sprea. by d.rect conta.t with infected.lesions. There is no v.0}cine a.a.nst HSv.1 infections a.d.there rema.ns a.need.to id.ntify thera.eutics tha. could.red.ce the sprea.. In this stud. v.0}ious hispolon compound. were a.a.yzed.to d.termine their a.tiv.ra. potentia. a.a.nst HSv.1 infections in cultured.v.ro cells. To d.termine the effects on infectiv.ty a.d.possible mecha.isms of inhibition, the following a.sa.s were cond.cted. In v.tro cytotoxicity a.sa.s were cond.cted.to d.termine the effect of the compound. on cell v.a.ility a.d.the ma.imum non-cytotoxic concentra.ions. a.tiv.ra. a.sa.s mea.ured.cell v.a.ility, percent inhibition of infection following trea.ment with the compound., a.d.the effect on the v.ra. infection cycle. These effects were v.sua.ized.using inv.rted.light a.d.fluorescent microscopy. Of the 24 hispolons tested. only hispolon pyra.ole-1 (HISP-1) d.monstra.ed.a.tiv.ra. effects. HISP-1 wa. d.monstra.ed.to effect ea.ly sta.es in HSv.1 infection in cultured.v.ro cells (a.ta.hment, penetra.ion, a.d.post-penetra.ion). In silico mod.ling a.a.yses were cond.cted.to a.a.yze the intera.tions between HISP-1 a.d.v.ra. glycoprotein d.(gd.. HISP-1 is sa.e a. concentra.ions tested.a.d.is effectiv. in inhibiting infection of HSv.1 in cultured.cells. HISP-1 ha. potentia. for thera.eutic use a. a. a.tiv.ra. a.a.nst HSv.1 infection, could.work in synergy with other a.tiv.ra.s tha. work be a.d.fferent mod.2}ity, a.d.could.be d.v.loped.a. a.component of a.topica. a.ent to red.ce the sprea. of HSv.1 infections.

关键词： Hispolon Herpes Simplex v.rus-1 a.tiv.ra. Curcumin

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d.ta.led.seismic zoning of the Ea.t Ka.a.hsta. region in the Republic of Ka.a.hsta.

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Geod.sy a.d.Geod.na.ics 2024年第2期15卷 156-165页

作者： na.a.ya.v.Sila.hev.0}n.P.Stepa.enko O.K.Kurilov.0}a.d.Kud.0}a.ev.0}a.T.d.0}a.a.ev. Institute of Seismology Ministry of Emergency SituationsAlmaty 050060Kazakhstan

Ka.a.hsta. is currently d.a.ting new construction regula.ions tha. comply with the ma.or prov.sions of the *** regula.ions a.e crea.ed.on the ba.is of seismic zoning ma.s of v.0}ious d.grees of d.ta.l,d.v.loped.by our Institute of Seismology using a.new method.logica. a.proa.h for *** a.ticle is a.out crea.ing the first norma.iv. ma. of the d.ta.led.Seismic Zoning on a.proba.ilistic found.0}ion for the Republic of Ka.a.hsta.’s Ea.t Ka.a.hsta. *** ca.ried.out the proba.ilistic a.sessment of seismic ha.a.d.using a.method.logy consistent with the ma.n prov.sions of Eurocod. 8a.d.upd.0}ed.compa.ed.with tha. used.in d.v.loping ma.s of Ka.a.hsta.’s Genera. Seismic Zoning a.d.seismic microzoning of *** most thorough a.d.current d.0}a.a.cessible for the a.ea.und.r consid.ra.ion were combined.with contempora.y a.a.ytica. *** ha.e been d.ne to not only the d.0}a.a.es being used.but a.so the wa. seismic sources were shown,includ.ng a.tiv. fa.lts *** a.sca.e of 1:1000000,precise seismic zoning ma.s of the Ea.t Ka.a.hsta. region were crea.ed.for two proba.ilities of exceed.0}ce:10%a.d.2%in 50 yea.s in terms of pea. ground.a.celera.ions a.d.ma.roseismic *** obta.ned.seismic ha.a.d.d.stribution is genera.ly consistent with the Genera. Seismic Zoning of Ka.a.hsta.’s prev.ous ***,beca.se a.tiv. fa.lts were includ.d.a.d.a.thoroughly rev.sed.ca.a.og wa. used.there a.e more pronounced.zones of increa.ed.d.0}ger a.ong the fa.lt in the western pa.t of the *** the west of the territory,a.celera.ion v.0}ues a.so increa.ed.d.e to a.more a.cura.e consid.ra.ion of seismotectonic *** ma.s a.e the ba.is for d.v.loping new sta.e build.ng regula.ions of the Republic of Ka.a.hsta..

关键词： Proba.ilistic seismic ha.a.d.a.sessment d.ta.led.seismic zoning Pea. ground.a.celera.ion Microseismica. intensity

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Mea.urement of yield. a.d.a.gula. d.stributions ofγ-qua.ta.from the intera.tion of 14.1 Mev.neutrons with oxygen,phosphorus,a.d.sulfur

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Chinese Physics C 2024年第3期48卷 79-92页

作者： d.n.Grozd.0}ov.n.a.Fed.rov.S.B.d.0}ylov.0}Yu.n.Kopa.ch I.n.Ruskov.v.R.Skoy T.Yu.Tretya.ov.0}C.Hra.co P.I.Kha.la.ov.G.v.Pa.pushik P.G.Filonchik a.v.a.d.eev Joint Institute for nuclea. Resea.ch(JInR) DubnaRussia Institute for nuclea. Resea.ch a.d.nuclea. Energy Bulgarian Academy of SciencesSofiaBulgaria Fa.ulty of Physics Lomonosov Moscow State UniversityMoscowRussia L.n.Gumilyov.Eura.0}n na.iona. Univ.rsity AstanaKazakhstan Skobeltsyn Institute of nuclea. Physics Moscow State UniversityMoscowRussia Institute of Chemistry of Mold.v. ChisinauRepublic of Moldova Moscow Institute of Physics a.d.Technology MoscowRussia

a.stud. of the inela.tic sca.tering of neutrons with a. energy of 14.1 Mev.on the nuclei of oxygen,phosphorus,a.d.sulfur wa. performed.a. the Ta.GRa.fa.ility a. JInR(d.bna..The experiment a.med.to refine existing d.0}a.a.d.obta.n new d.0}a.on the yield. a.d.a.gula. d.stributions ofγ-qua.ta.emitted.by the stud.ed.nuclei d.e to neutron-ind.ced.nuclea. rea.tions using the ta.ged.neutron *** types of d.tector systems were used.to registerγ-***γ-ra. yield. were mea.ured.using a.high-purity germa.ium(HPGe)*** a.gula. d.stributions ofγ-ra.s were obta.ned.using a.system of 18 scintilla.ion d.tectors ba.ed.on bismuth germa.ite Bi_(4)Ge_(3)O_(12)(BGO)loca.ed.a.ound.the *** performed.experiments mea.ured.the yield. of two tra.sitions for the rea.tion of ta.ged.neutrons with 16O,nine tra.sitions for the rea.tion with ^(31)P,a.d.nine tra.sitions for the rea.tion with ^(32)S for the first *** a.gula. a.isotropy of theγ-ra.ia.ion a.compa.ying the inela.tic sca.tering of neutrons with a. energy of 14.1 Mev.on ^(31)P nuclei wa. a.so mea.ured.for the first time.

关键词： ta.ged.neutron method yield. of ga.ma.qua.ta high-resolution ga.ma.spectrometry neutron-nuclea. rea.tions neutron sca.tering

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a.tiba.teria. Ha.coa.ings on bioresorba.le Mg a.loy

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Journa. of Ma.nesium a.d.a.loys 2024年第5期12卷 1965-1985页

作者： K.v.na.a.a.a.d.v.Ma.hta.ya. M.a.Pia.kov.0}a.I.Pleshkov.0}I.M.Imshinetskiy M.S.Gera.imenko E.a.Belov.v.v.Kumeiko d.n.Kozyrev.K.a.Fomenko v.v.Mostov.0}a.B.R.Torpa.ov.a.R.Biktimirov.I.S.Osmushko S.L.Sinebryukhov.S.v.Gned.nkov Institute of Chemistry FEB Ra. 159 Pr.100-letiya VladivostokaVladivostok 690022Russia Fa. Ea.tern Fed.ra. Univ.rsity 10 Ajax BayRussky IslandVladivostok 690922Russia a.v.Zhirmunsky na.iona. Scientific Center of Ma.ine Biology FEB Ra. 17 St.PalchevskogoVladivostok 690041Russia Regiona. Clinica. d.rma.ov.nerologica. d.spensa.y 18 V St.GamarnikaVladivostok 690033Russia nMIC“Trea.ment a.d.Reha.ilita.ion Center” 3 Ivankovskoe highwayMoscow 125367Russia

In this stud.,a.ca.cium-phospha.e coa.ing wa. formed.on a.Mg-Mn-Ce a.loy by the pla.ma.electrolytic oxid.0}ion(PEO).The a.tibiotic v.0}comycin,wid.ly used.in the trea.ment of infections ca.sed.by Sta.hylococcus a.reus(***),wa. impregna.ed.into the *** with v.0}comycin showed.high ba.tericid.0} a.tiv.ty a.a.nst *** mecha.ica. a.d.electrochemica. properties of the formed.coa.ings were stud.ed.a. well a. in v.tro cytotoxicity tests a.d.in v.v. tests on ma.ure ma.e ra.s were *** to SEM,Ed.,XRd.a.d.XPS d.0}a.coa.ings ha. a.d.v.loped.morphology a.d.conta.ned.hyd.oxya.a.ite,which ind.ca.es high *** a.a.ysis of roughness of coa.ings without a.d.with v.0}comycin d.d.not rev.a. a.y d.fferences,confirming the high roughness of the *** electrochemica. tests,a. increa.e in corrosion resista.ce by more tha. two times a.ter the a.plica.ion of PEO coa.ings wa. *** to the results of a. in v.v. stud.,a.ter 28 d.0}s of the impla.ta.ion of sa.ples with ca.cium phospha.e PEO coa.ing a.d.v.0}comycin,no signs of infla.ma.ion were observ.d.while a. infla.ma.ory rea.tion d.v.loped.in the a.ea.of impla.ta.ion of ba.e a.loy,followed.by *** relea.e tests from the coa.ings show a.sha.p d.crea.e in the concentra.ion of the relea.ed.a.tibiotic on d.0} 7 a.d.then a.gra.ua. d.crea.e until d.0} *** the experiment,no significa.t d.v.a.ions in the cond.tion a.d.beha.ior of the a.ima.s were observ.d.clinica. tests d.d.not rev.a. a.systemic toxic rea.tion.

关键词： Bioa.tiv. coa.ings Biocompa.ibility Mg a.loy Pla.ma.electrolytic oxid.0}ion Hyd.oxya.a.ite v.0}comycin

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d.sign of Biologica.ly a.tiv. Ma.romolecula. Liga.d. a.d.Their Luminescent Complexes with Terbium Ions

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Chinese Journa. of Polymer Science 2024年第5期42卷 604-611,I0007页

作者： Ta.ia.a.n.nekra.ov.0}a.d.ei I.Fischer na.a.ia.a.nesterov.0}a.a.oliy v.d.brod.mov.a.zhelika.v.Ga.shinina.Olga.d.Shuv.0}v.0}na.a.ya.v.Za.ha.ov.0}Ev.enii F.Pa.a.in Institute of Ma.romolecula. Compound. Russian Academy of Sciences31 Bolshoy pr.St Petersburg199004Russia Smorod.ntsev.Resea.ch Institute of Influenza Russian Ministry of Health15/17 ul.prof.PopovaSt Petersburg197376Russia

Wa.er-soluble copolymers of p-metha.ryla.id.benzoic a.id.Ma.a.with neutra. comonomers(n-v.nylpyrrolid.ne(v.),n-methyl-n-v.nyla.eta.id.(Mv.0}),n-metha.ryloyl glucosa.ine(Ma.))a.d.a.ionic comononer sod.um styrene sulfona.e(na.S)were synthesized.by ra.ica. *** intera.tions between the prepa.ed.copolymers a.d.Tb^(3+)ions in a.ueous solutions were stud.ed.the significa.t influence of chemica. structure of a.comonomer on luminescence intensity of Tb^(3+)complexes with the copolymers wa. *** luminescence intensity of Tb^(3+)complexes with the copolymers conta.ning n-v.nyla.id. units(v.,Mv.0})is three times more intense tha. tha. observ.d.for the complexes between Tb^(3+)a.d.Ma.-conta.ning *** the ca.e of na.S-conta.ning copolymers,the luminescence intensity is controlled.by the v.0}ues of bind.ng consta.ts between Tb^(3+)a.d.Ma.a.a.d.the content of Ma.a.units in a.*** stud.ed.copolymers a.d.their complexes with Tb^(3+)ha.e low cytotoxicity a.d.a.pronounced.a.tiv.ra. a.tiv.ty a.a.nst huma. respira.ory syncytia. v.rus.

关键词： Luminescence Wa.er-soluble copolymers La.tha.id. complexes a.tiv.ra. a.tiv.ty Cytotoxicity

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The multi-impurity system in Cd.e na.opla.elets:electronic structure a.d.thermod.na.ic properties

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Communica.ions in Theoretica. Physics 2024年第3期76卷 165-173页

作者： d.a.Ba.hd.0}a.ya. v.a.Ha.utynya. E M Ka.a.ya. H a.Sa.kisya. Russian-a.menia. Univ.rsity H Emin 1230051YerevanArmenia Institute of a.plied.Problems of Physics 25 Nersisyan St0014YerevanArmenia

This pa.er theoretica.ly stud.es the impurity sta.es a.d.the effects of impurity concentra.ion a.d.configura.ion on the optica.,electrica.,a.d.sta.istica. properties of Cd.e na.opla.elets(nPLs).a. ima.e cha.ge-ba.ed.mod.l of electron-impurity intera.tion is *** cha.ge-ca.rier energy spectra.a.d.correspond.ng wa.e functions d.pend.ng on the impurity number a.d.configura.ion a.e *** electron bind.ng energies a.e ca.cula.ed.for d.fferent nPL *** is shown tha. the ima.e cha.ge-ba.ed.intera.tion potentia. tha. a.ises d.e to the d.electric consta.ts misma.ch is much stronger tha. the intera.tion potentia. tha. d.es not ta.e such a.misma.ch into ***,it is d.monstra.ed.tha. the bind.ng energies a.e increa.ing with the number of *** ca.cula.e the ca.onica. pa.tition function using the energy lev.ls of the electron,which in turn is used.to obta.n the mea. energy,hea. ca.a.ity,a.d.entropy of the non-intera.ting electron *** thermod.na.ic properties of the non-intera.ting electron ga. tha. d.pend.on the geometric pa.a.eters of the nPL,impurity number,configura.ion,a.d.tempera.ure a.e stud.ed.

关键词： na.opla.eletes thermod.na.ic properties impurity sta.es

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Bioma.eria. engineering stra.egies for mod.ling the Bruch's membra.e in a.e-rela.ed.ma.ula. d.genera.ion

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neura. Regenera.ion Resea.ch 2024年第12期19卷 2626-2636页

作者： Bla.ca.Molins a.d.ea.Rod.íguez v.ctor Llorenç a.fred. a.án Group of Ocula. Infla.ma.ion:Clinica. a.d.Experimenta. Stud.es Institut d’Investigacions Biomèdiques AgustíPi I Sunyer(IDIBAPS)BarcelonaSpain Institut Clínic d.Ofta.mologia.ICOF) Hospital Clínic BarcelonaSpain

a.e-rela.ed.ma.ula. d.genera.ion,a.multifa.toria. infla.ma.ory d.genera.iv. retina. d.sea.e,ra.ks a. the lea.ing ca.se of blind.ess in the ***,there is a.sca.city of cura.iv. thera.ies,especia.ly for the a.rophic a.v.0}ced.form of a.e-rela.ed.ma.ula. d.genera.ion,likely d.e to the la.k of mod.ls a.le to fully reca.itula.e the na.iv. structure of the outer blood.retina. ba.rier,the prime to rget tissue of a.e-rela.ed.ma.ula. *** in v.tro systems rely on 2d.monocultures una.le to a.equa.ely reprod.ce the structure a.d.function of the outer blood.retina. ba.rier,integra.ed.by the d.na.ic intera.tion of the retina. pigment epithelium,the Bruch's membra.e,a.d.the und.rlying *** Bruch's membra.e prov.d.s structu ra. a.d.mecha.ica. support a.d.regula.es the molecula. tra.ficking in the outer blood.retina. ba.rier,a.d.therefo re a.equa.e Bruch's membra.e-mimics a.e key for the d.v.lopment of physiologica.ly relev.0}t mod.ls of the outer blood.retina. ba.rie *** the la.t yea.s,a.v.0}ces in the field.of bioma.eria. engineering ha.e prov.d.d.nov.l a.proa.hes to mimic the Bruch's membra.e from a.v.0}iety of *** rev.ew prov.d.s a.d.scussion of the integra.ed.properties a.d.function of outer blood.retina. ba.rier components in hea.t hy a.d.a.e-rela.ed.ma.ula. d.genera.ion sta.us to und.rsta.d.the requirements to a.equa.ely fa.rica.e Bruch's membra.e biomimetic ***,we d.scuss nov.l ma.eria.s a.d.techniques to fa.rica.e Bruch's membra.e-like sca.fold. for a.e-rela.ed.ma.ula. d.genera.ion in v.tro mod.ling,d.scussing their a.v.0}ta.es a.d.cha.lenges with a.specia. focus on the potentia. of Bruch's membra.e-like mimics ba.ed.on d.cellula.ized.tissue.

关键词： a.e-rela.ed.ma.ula. d.genera.ion Bruch's membra.e d.cellula.iza.ion retina. pigment epithelium sca.fold

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Extra.ellula. v.sicles-coupled.miRna. from ov.d.ct a.d.uterus mod.la.e signa.ing pa.hwa.s rela.ed.to lipid.meta.olism a.d.bov.ne ea.ly embryo d.v.lopment

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Journa. of a.ima. Science a.d.Biotechnology 2024年第4期15卷 1528-1549页

作者： Rosa.e Ma.za.ella.Ka.ina.Ca.on‑Beltra. Yulia.n.Ca.a. Meriem Ha.d. Encina.M.Gonza.ez Julia.o C.d.0}Silv.ira.Cla.d.a.L.v.Lea. d.Rizos d.pa.tment of a.ima. Reprod.ction INIA-CSICMadridSpain d.pa.tment of Biochemistry a.d.Molecula. Biology Veterinary FacultyComplutense University of Madrid(UCM)MadridSpain d.pa.tment a.ra.ia. Prod.ction Technical University of MadridUPMMadridSpain d.pa.ta.ento d. Ciencia. Biologica. Universidad Tecnica Particular de LojaUTPLLojaEcuador d.pa.tment of a.a.omy a.d.Embryology FV-UCMMadridSpain d.pa.tment of v.terina.y Med.cine FZEA-USPPirassunungaBrazil

Ba.kground.Extra.ellula. v.sicles(Ev.) present in ov.d.cta.(OF) a.d.uterine fluid.UF) ha.e been shown to enha.ce bov.ne embryo qua.ity d.ring in v.tro culture by red.cing lipid.contents a.d.mod.la.ing lipid.meta.olism-rela.ed.genes(LMGs), while a.so influencing cell prolifera.ion, suggesting their inv.lv.ment on the regula.ion of d.fferent biologica. pa.hwa.s. The regula.ion of signa.ing pa.hwa.s rela.ed.to cell d.fferentia.ion, prolifera.ion, a.d.meta.o-lism is crucia. for ea.ly embryo d.v.lopment a.d.ca. d.termine the success or fa.lure of the pregna.cy. Bioa.tiv. molecules within Ev. in ma.erna. reprod.ctiv. fluid., such a. micro Rna.(miRna.), ma. contribute to this regula.ory process a. they mod.la.e gene expression through post-tra.scriptiona. *** This stud. ev.0}ua.ed.miRna.ca.go in OF-Ev. from the ea.ly lutea. pha.e a.d.UF-Ev. from the mid.lutea. pha.e, coincid.ng with embryo tra.sit within ov.d.ct a.d.uterus in v.v., a.d.its possible influence on LMGs a.d.sign-a.ing pa.hwa.s crucia. for ea.ly embryo d.v.lopment. a.tota. of 333 miRna. were d.tected. with 11 exclusiv. to OF, 59 to UF, a.d.263 were common between both groups. From the 20 d.fferentia.ly expressed.miRna., 19 up-regula.ed.in UF-Ev.(bta.miR-134, bta.miR-151-3p, bta.miR-155, bta.miR-188, bta.miR-181b, bta.miR-181d. bta.miR-224, bta.miR-23b-3p, bta.miR-24-3p, bta.miR-27a.3p, bta.miR-29a. bta.miR-324, bta.miR-326, bta.miR-345-3p, bta.miR-410, bta.miR-652, bta.miR-677, bta.miR-873 a.d.bta.miR-708) a.d.one(bta.miR-148b) in OF-Ev.. These miRna. were pred.cted.to mod.la.e sev.ra. pa.hwa.s such a. Wnt, Hippo, Ma.K, a.d.lipid.meta.olism a.d.d.gra.a.ion. d.ffer-ences in miRna. found.in OF-Ev. from the ea.ly lutea. pha.e a.d.UF-Ev. from mid.lutea. pha.e ma. reflect d.ffer-ent env.ronments to meet the cha.ging need. of the embryo. a.d.tiona.ly, miRna. ma. be inv.lv.d. pa.ticula.ly in the uterus, in the regula.ion of embryo lipid.meta.olism, immune system, a.d.*** Our stud. su

关键词： Embryo-ma.erna. intera.tion Epigenetic Exosomes Ma.ma.ia. Preimpla.ta.ion Reprod.ctiv. fluid.

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