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Pig Reproductive and Respiratory Syndrome Virus (PRRSV) Does not Attach to Boar Sperm;It Affects Only the Velocity Pattern and the mobility Pattern

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Journal of Biosciences and medicines 2024年第4期12卷 216-228页

作者： néstor méndez palacios netzi naidí mendez palacios Felicitas Vázquez Flores José Alfredo Galicia Domínguez Edgar Guadalupe Beltrán Rosas maximino méndez mendoza Facultad de medicina Veterinaria and Zootecnia-Benemé rita Universidad Aut&#243noma de Puebla Puebla Mexico Independent Advisor in the Porcine Area Puebla Mexico

The purpose of the study was to evaluate the sperm viability of semen infected with PRRSV viral particles, observing the effect of the Virus on the motility of boar sperm. The work was carried out at the FmVZ-BUAP Genetics and Reproduction Laboratory. 5 stallions were used. Each sample contained 1 × 106 sperm, the PRRS virus strain was ATCC-VR-2332 (0, 102, 104 and 106 copies of RnA/mL in triplicate), it was observed daily at the CASA;Hamilton Thorne®. Cells with mT (P n days 1, 3, 5, 7 and 10 of evaluation with 201 ± 7.3, 167 ± 10.1, 165 ± 14.6, 134 ± 8.2 and 120 ± 8.8, respectively. The % mP between control and virus concentrations (P ≥ 0.05). The LCV on day 1 and 7 PI at 10X2 and 10X6 (P ntrol. In the Correlation matrix, where it is observed that there is a correlation between VSL and VAP, VSL and VCL, VCL and ALH, VAP with ALH. There is a correlation of VSL and ALH, STR and ALH. In this study there were (P ≤ 0.01) in the VCL, in the concentrations (102) 162.81 ± 10.65 and (106) 177.12 ± 5.77 vs 193.04 ± 4.62 of control. This indicates that altering these parameters would be related to fertility and the PRRS virus affects the LCV. Regarding the VSL, it was observed that the sperm infected with viruses 102, 104 and 106 of 48.00 ± 3.38, 49.88 ± 1.83 and 50.55 ± 2.24 Vs. 56.66 ± 1.68 of control respectively, the control would have greater possibilities of fertilizing the oocyte. In this study, it was found (P ≤ 0.01) in the VAP with 102 of 77.26 ± 5.16, 104 with 83.35 ± 2.41 and 106 with 81.29 ± 3.14 vs the control with 90.56 ± 2.07. Regarding the ALH there is (P 4 with 8.70 ± .26 and 106 with 9.64 ± 0.23 vs control 8.50 ± 0.27. The presence of different concentrations of PRRSV in boar semen induces changes in different types of sperm motility. Infection of ej

关键词： PRRSV Boar Sperms Velocity Pattern and mobility Pattern

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Glacier reconstruction of La Covacha massif in Sierra de Gredos(central Spain) during the Last Glacial maximum

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Journal of mountain Science 2019年第6期16卷 1336-1352页

作者： néstor CAmPOS David palacios Luis m.TAnARRO Department of Geography Complutense University of Madrid

A paleoglacier reconstruction during the Last Glacial maximum(LGm) is presented for a high mountain area of La Covacha massif in Sierra de Gredos(Iberian Central System) in central Spain. The moraines that, according with the previous literature, had formed during the global LGm, were mapped through photo interpretation of digital aerial photographs and 3D images and detailed field surveys. The topography of the paleoglaciers was estimated using a simple steady-state model that assumed a perfectly plastic ice rheology, reconstructing the theoretical ice profiles and obtaining the ice thickness of the glaciers during the LGm. The reconstruction of the glaciers was carried out using automated and semi-automated physically-based models, obtaining more realistic results with semi-automated models. According to our study, the paleoglaciers in the study area covered an area of 34.79 km^2 during the global LGm, with a maximum ice thickness of 366 meters in La Vega gorge, a total volume of 34.25 × 108 m^3 and a mean paleoELA of 1932 meters. most of the ice(~82%) was in paleoglaciers facing north, and the rest was in paleoglaciers with other orientations. This emphasizes the importance of orientation in relation to glacier dynamics and ice accumulation. The results obtained in the calculation of paleoELAs during the global LGm in Gredos are average compared to the Iberian mountains of the northwest where the values were much lower, and with respect to those of the Southwest, much higher. This demonstrates the importance of the exposure of mediterranean mountains to the humidity sources coming from the nW during the global LGm, as is the case at present.

关键词： Glacier reconstruction Paleoglacier Ice thickness Equilibrium line altitude Iberian Central System

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microstructural Characterization of Large Area C<sub>60</sub>Films Obtained by Conventional microwave Oven Irradiation

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World Journal of nano Science and Engineering 2012年第4期2卷 213-218页

作者： Jacobo martínez-Reyes Lucia Graciela Díaz Barriga-Arceo Luis Rendón-Vazquez Reynaldo martínez-Guerrero néstor Romero-Partida Eduardo palacios-González Vicente Garibay-Febles Jaime Ortiz-López ImP-molecular Engineering Program México City México national Polytechnic Institute (IPn) ESFM UPALM México City México national Polytechnic Institute (IPn) ESIQIE UPALM México City México ROmFER S.A. de C.V. México City México UnAm-FCiencias México City México

In the present work the synthesis of C60 produced in a conventional microwave oven from the decomposition of camphor resin is reported. The polycrystalline structure of the sample was determined by X-Ray Diffraction (XRD), the sample showed several phases, the main phase corresponds to fullerene C60 ordered in a Face-Centered Cubic structure (FCC), with two more structures: one orthorhombic system and the other the monoclinic system coexisting also with graphite 2H phase. It was observed in a Scanning Electron microscopy (SEm), that the sample formed thin films of stacked carbon. Whereas in a High Resolution Transmission Electron microscopy (HRTEm), measurements in Bright Field mode revealed that the main phase of the material is C60 ordered in FCC structure and the elemental composition and atomic bonding state can be determined by analyzing the energy with the electron microscope by Elesctron Energy- Loss Spectroscopy (EELS), technique allowed confirm all the phase C60 established with XRD observations.

关键词： microwave-Assisted Synthesis Carbon Film Fullerene

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