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Asian Pacific Journal of Tropical Medicine 2016年第8期9卷 720-726页

作者： Jong-oh Kim Jae-Ok Kim Wi-Sik Kim myung-joo oh Department of Aqualife Medicine College of Fisheries and Ocean Science Chonnam National University

Objective:To develop the rapid and efficient quantitative detection tool for nervous necrosis virus isolated from sevenband grouper Hyporhodus ***:The viral genes of the NNV(SGYeosu08) isolated from sevenband grouper were phylogenetically *** addition,novel quantitative PCR primers based on the genomic sequence of SGYeosu08 isolate were designed and compared it with the conventional bio-assay method(TCID_(50)) using in vitro and in vivo ***:The phylogenetic analysis of viral genes demonstrated the relationship of SGYeosu08 with members of red-spotted grouper nervous necrosis virus(RGNNV).The qNNV_Rl primer set(R1_F and R1_R) and the qNNV_R2 primer set(R2_F and R2_R) revealed 93%primer efficiency(regression:y=-0.2861 x + 9.9401,R^2= 0.9976)and the revealed 108%primer efficiency(regression:y=-0.3172 x + 10.0611,R^2= 0.9982),*** comparison with viral infectivity calculated by TCID_(50) method showed similar kinetic pattern at in vitro and NNV challenged fish(in vivo) ***:Result show that this method is rapid and efficient to diagnose NNV infection compare to traditional bioassay method(TCID_(50)).

关键词： Nervous necrosis virus(NNV) Quantitative detection Diagnostic Sevenband grouper

Detection of coat protein gene of nervous necrosis virus using loopmediated isothermal amplification

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Asian Pacific Journal of Tropical Medicine 2016年第3期9卷 230-235页

作者： Jinik Hwang Sung-Suk Suh Mirye Park myung-joo oh Jong-oh Kim Sukchan Lee Taek-Kyun Lee South Sea Environment Research Department Korea Institute of ocean science & technology Korea University of Science and Technology Department of Aqualife Medicine Chonnam National University Department of Genetic Engineering Sungkyunkwan University

Objective:To establish a novel and highly specific loop-mediated isothermal amplification(LAMP) assay for the identification of nervous necrosis virus(NNV) ***:A set of synthesized primers was used to match the sequences of a specific region of the nnr gene from the National Center for Biotechnology Information database,not originating from NNV-infected fish,the efficiency and specificity of LAMP were measured dependent on the concentration of DNA polymerase and the reaction temperature and *** addition,to determine species-specific LAMP primers,cross reactivity testing was applied to the reaction between NVV and other virus families including viral hemorrhagic septicemia virus and marine ***:The optimized LAMP reaction carried out at 64 ℃ for 60 min,and above 4 U Bst DNA *** sensitivity of LAMP for the detection of nnv was thus about 10 times greater than the sensitivity of polymerase chain *** LAMP assay primers were specific for the detection NNV infection in Epinephelus ***:The development of LAMP primers based on genetic information from a public database,not virus-infected samples,may provide a very simple and convenient method to identify viral infection in aquatic organisms.

关键词： Nervous necrosis virus Nodaviridae Polymerase chain reaction Loop-mediated isothermal Amplification

Development of Fifteen Novel Microsatellite Markers from Rock Bream (<i>Oplegnathus fasciatus</i>)

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Open Journal of Genetics 2014年第4期4卷 287-291页

作者： Jong-oh Kim Wi-Sik Kim myung-joo oh Department of Aqualife Medicine Chonnam National University Yeosu Korea

Rock bream (Oplegnathus fasciatus) is one of the most economically valuable fish in Korea. In recent years, artificial breeding techniques with molecular and microsatellite markers have been developed to enhance rock bream resources. Microsatellite loci to define genetic diversity were screened in rock bream (n = 30) from Jeju areas of Korea and fifteen polymorphic microsatellite loci were newly identified and analyzed. The number of alleles per locus ranged from 9 to 34 while observed and expected heterozygosity ranged from 0.600 to 1.000 and from 0.772 to 0.977, respectively. These markers will serve as a foundation for future population genetic studies and the selective breeding technology of rock bream farming.

关键词： Oplegnathus fasciatus Rock Bream Microsatellite Markers Population Genetics

Evaluation of leaching characteristic and kinetic study of lithium from lithium aluminum silicate glass-ceramics by Naoh

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Journal of Environmental Sciences 2021年第9期33卷 98-110页

作者： Dongseok Lee Sung-Ho joo Dong Ju Shin Shun myung Shin Resources Recovery Research Center Korea Institute of Geoscience and Mineral Resources(KIGAM)124 Gwahak-roYuseong-guDaejeon 34132Republic of Korea Department of Resources Recycling University of Science and Technology(UST)217Gajeong-roYuseong-guDaejeon 34113Republic of Korea

The behavior and mechanism of Li leaching from lithium aluminum silicate glass-ceramics which can be used as a secondary source of Li using aqueous Naoh solution was *** Li leaching efficiency is increased with increasing concentration of Naoh, specific surface area, and reaction *** leached under optimum conditions, 2 mol/L Naoh, 53 μm particle undersize, 1:10 solid/liquid ratio, 250 r/min stirring speed, 100℃ reaction temperature, 12 hr, the Li leaching efficiency was approximately 70%.However, when the leaching experiment was performed for 48 hr, the concentration of Li+ ions contained in the leach liquor decreased from 1160 to 236 mg/*** investigate the origin of this phenomenon, the obtained leach residue was analyzed by X-ray diffraction, scanning electron microscopy, and energy-dispersive X-ray *** analyses show that zeolite was formed around the lithium aluminum silicate glass-ceramics, which affected the leaching of by adsorbing Li+ *** addition, using the shrinking-core model and the Arrhenius equation, the leaching reaction with Naoh was found to depends on the chemical reaction of the two reactants, with a higher than 41.84 kJ/mol of the activation energy.

关键词： Lithium aluminum silicate glass-ceramics Naoh Lithium Leaching

Evaluation of the Mrp2-mediated flavonoid-drug interaction potential of quercetin in rats and in vitro models

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Asian Journal of Pharmaceutical Sciences 2019年第6期14卷 621-630页

作者： Ju-Hee oh joo Hyun Lee Young-joo Lee Division of Biopharmaceutics College of PharmacyKyung Hee UniversitySeoul 02447Republic of Korea Department of Life and Nanopharmaceutical Sciences College of PharmacyKyung Hee UniversitySeoul 02447Republic of Korea

Quercetin is a biologically active flavonoid that has been used as a popular health *** is reported that quercetin may cause flavonoid-drug interaction mediated by P-glycoprotein,the most predominant efflux *** this study,we comprehensively evaluated the potential of the pharmacokinetic interaction of quercetin mediated by multidrug resistance-associated protein 2(MRP2),another major efflux ***2-transfected MDCKII cells and LS174T cells were used to evaluate the potential inhibition and induction of MRP2 by quercetin in *** evaluate the induction effect of quercetin on Mrp2 in vivo,Mrp2 mRNA expression in rat liver,kidney,and small intestinal tissues was determined after the oral administration of quercetin(50,100,or 250 mg/kg)for seven ***2-mediated interaction potential was also evaluated by the pharmacokinetic study of phenolsulfonphthalein in rats after single or multiple doses of ***,the effect of quercetin on absorption of docetaxel,a P-glycoprotein and CYP3A4 substrate,was also *** inhibited the function of MRP2 at 10μM and induced the mRNA expression of MRP2 at 50μM in ***,at 100 mg/kg,quercetin markedly increased Mrp2 expression in the small intestine of ***,there was no significant change in phenolsulfonphthalein pharmacokinetics due to single-(50,100,or 250 mg/kg)or multiple-dose(50,100,or 250 mg/kg for seven days)quercetin *** contrast,a significant interaction caused by quercetin(100 mg/kg)was observed in the absorption of *** results suggested that although quercetin modulates the function and expression of MRP2 in vitro,it may have a low potential of Mrp2-mediated interaction and present negligible safety concerns related to the interaction.

关键词： Quercetin P-glycoprotein Multidrug resistance-associated protein 2 Pharmacokinetics Flavonoid-drug interaction

Selective catalytic NO_x reduction on Antimony promoted V_2O_5-Sb/TiO_2 catalyst

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Rare Metals 2006年第Z2期25卷 84-88页

作者： HA Heon Phil CHUNG Soon Hyo oh Young joo Metal Processing Research Center Korea Institute of Science and Technology Cheongryang Seoul 130-650 Republic of Korea

Quantum chemical calculation was carried out to choose a promoter which can reduce the poisoning of V2O5/TiO2 catalysts by SO2. Several atoms were chosen as candidates and new catalysts were synthesized by impregnation method. The NOx conversion rate was measured at temperatures between 100 and 400 ℃ and poisoning effect was investigated. The most promising candidate promoter, Se, was excluded because of its high vapor pressure. On the other hand, Sb shows best promoting properties. Sb promoted catalyst reaches the maximum NOx conversion rate at 250 ℃. It also shows considerably enhanced resistance to poisoning of V2O5/TiO2 catalysts by SO2.

关键词： De NOx selective catalytic reduction SO2 poisoning low temperature activity

Periportal thickening on magnetic resonance imaging for hepatic fibrosis in infantile cholestasis

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World Journal of Gastroenterology 2020年第21期26卷 2821-2830页

作者： myung Hwan Lee Hyun joo Shin Haesung Yoon Seok joo Han Hong Koh Mi-Jung Lee Department of Radiology Severance HospitalSeverance Pediatric Liver Disease Research GroupResearch Institute of Radiological ScienceYonsei University College of MedicineSeoul 03722South Korea Department of Surgery Severance HospitalSeverance Pediatric Liver Disease Research GroupYonsei University College of MedicineSeoul 03722South Korea Division of Gastroenterology Hepatology and NutritionDepartment of PediatricsSeverance Children’s HospitalSeverance Pediatric Liver Disease Research GroupYonsei University College of MedicineSeoul 03722South Korea

BACKGROUND Untreated neonatal cholestasis can progress to liver cirrhosis and end stage liver disease in infancy due to prolonged hepatocyte and biliary tree injury and may require liver ***,non-invasive evaluation of hepatic fibrosis is important in infants with *** To investigate the usefulness of periportal thickening(PT)measured on liver magnetic resonance imaging(MRI)for the assessment of hepatic fibrosis in infants with cholestasis including biliary atresia(BA).METHODS This retrospective study included infants less than 6 mo who underwent liver MRI and biopsy for the evaluation of infantile *** and spleen size were measured on *** assessment was based on aspartate transaminase to platelet ratio index(APRI).The grade of histopathologic fibrosis was assessed by the METAVIR grading *** and diagnostic performance of PT,normalized spleen size ratio(SR,using the upper normal size limit),and APRI for diagnosing hepatic fibrosis were obtained by receiveroperating characteristic(ROC)curve *** A total of 155 patients were included,110 of which were diagnosed with *** age at the time of MRI was 57.6±34.4 *** were positive correlations between fibrosis grade and PT and SR,even after adjusting age(all,P<0.001).For the diagnosis of significant fibrosis(METAVIR grade F2-F4),the area under the ROC curve was 0.899(95%CI:0.840–0.941)for PT(cutoff,4.2 mm),which was higher than 0.741(95%CI:0.664–0.808)for SR and 0.712(95%CI:0.634–0.782)for APRI(both,P<0.001).For the diagnosis of cirrhosis(F4),the area under the ROC curve was the highest with SR as 0.790(95%CI:0.718–0.852).CONCLUSION Liver MRI findings of PT and SR are useful to assess clinically significant hepatic fibrosis(F2 and higher)in infants with cholestasis including BA.

关键词： Infants Cholestasis Biliary atresia Liver Fibrosis Magnetic resonance imaging

Primary rectal mucosa-associated lymphoid tissue lymphoma treated with only endoscopic submucosal dissection:A case report

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World Journal of Clinical Cases 2023年第14期11卷 3362-3368页

作者： Wan-Sik Lee myung-Giun Noh Young-Eun joo Department of Internal Medicine Chonnam National University Medical SchoolHwasun-eup 58128South Korea Department of Pathology Chonnam National University Medical SchoolHwasun-eup 58128South Korea

BACKGROUND Mucosa-associated lymphoid tissue(MALT)lymphoma is a distinct subtype of non-Hodgkin B cell lymphoma that mostly involves the gastrointestinal *** stomach is the most commonly affected site whereas colorectal involvement occurs very *** its rarity,the management and clinical outcome of colorectal MALT lymphoma are not well established *** SUMMARY From the superficial capillary bed in the lower *** ultrasonography showed homogenous hypoechoic lesions in the deep mucosal *** submucosal dissection(ESD)was done for accurate histologic diagnosis and treatment and both the rectal lesions were completely removed en bloc and subsequently diagnosed as primary rectal MALT ***,we report a case of primary rectal MALT lymphoma in a 68-year-old woman that was treated by only ESD,and the 12-month follow-up revealed no tumour *** These results of our case and previous reports suggest that endoscopic resection alone may be a feasible and safe treatment for primary colorectal MALT lymphoma and allows organ preservation.

关键词： Rectum Mucosa-associated lymphoid tissue lymphoma Endoscopic submucosal dissection

Effect of Laser Parameters on Sintered Powder Morphology

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Journal of Materials Science & Technology 2010年第4期26卷 375-378页

作者： Byeong-Don joo Jeong-Hwan Jang Jae-Ho Lee Young-myung Son Young-Hoon Moon Engineering Research Center for Net Shape and Die Manufacturing School of Mechanical EngineeringPusan National University

Selective laser sintering is a kind of rapid prototyping process whereby a three-dimensional part is built layerwise by laser scanning a powder. This process is highly inffuenced by powder and laser parameters such as laser power, scan rate, spot size and layer thickness. Therefore a study on fabricating a line with Fe-Ni-Cr powder on AISI H13 tool steel has been performed by selective laser sintering. In this study, fabrication was performed by experimental facilities consisting of a 200W fiber laser which can be focused to 0.08 mm and atmospheric chamber which can control atmospheric pressure with argon. The line was fabricated with various laser power, scan rate and layer thickness. Line width and surface quality were investigated. With power increase or layer thickness decrease, line width was decreased and line surface quality was improved with scan rate optimization.

关键词： Selective laser sintering Direct fabrication Fiber laser

Laser surface hardening of AISI H13 tool steel

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中国有色金属学会会刊：英文版 2009年第4期19卷 917-920页

作者： Jae-Ho LEE Jeong-Hwan JANG Byeong-Don joo Young-myung SON Young-Hoon MOON School of Mechanical Engineering Pusan National University

An attempt was made to improve the surface hardness and wear properties of AISI H13 tool steel through solid solution hardening and refinement of microstructures using a 200 W fiber laser as a heat generating *** hardness of laser melted zone was *** order to identify the effect of heat input on the laser melting zone,scanning conditions were *** results show that,the hardness of as-received AISI H13 tool steel is approximately Hv 240,and the hardness after laser surface heat treatment is around Hv *** hardening depth and width are increased with the increase in the heat input *** of experimental results will be considered in tooling industry.

关键词：激光表面硬化工具钢 H13 激光表面热处理表面硬度信息社会光纤激光器固溶强化