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Studies on antimicrobial activity and brine shrimp lethality of crude samples of six different species of puffer ifshes

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Journal of Coastal Life Medicine 2015年第7期 515-517页

作者： Masilamani Mohan Raj Subramanian Bragadeeswaran Anbukkarasu Suguna muthuramalingam uthaya siva Centre of Advanced Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608502TamilnaduIndia

Objective:To evaluate the antimicrobial activity and brine shrimp lethality activity of six different species of puffer fishes,includingCyclichthys orbicularis,Diodon holocanthus,Canthigaster solandri,Arthron hispidus,*** andLagocephalua inermis(***).Methodology:The puffer fishes were collected from Annangkovil Fish Landing Centre(Lattitude 11°30.47'N;Longitude 79°47.02'E),Parangipettai,Southeast Coast of India during summer season because of *** tissue samples were collected from the clearly washed specimens,extracted with methanol at 37°C for 3 days and filtered through Whatman No.1 filter *** solvents such as methanol and ethanol were concentrated by using rotary evaporator under reduced *** dark brown gummy mass was stored at 4°C for further *** crude samples were analysed with human pathogens to assess the antibacterial activity and this was carried out by using standard disc diffusion *** brine shrimp lethality was calculated as the percentage of mortality which was firstly calculated by dividing the number of dead larvae by the total number and then multiplied to 100%.Results:The antibacterial activity of crude extract of puffer fishes were exhibited against 10 different human bacterial *** the ten human pathogens,Arthron hispidus showed maximum zone of inhibition(8 mm)againstStaphylococcus aureus *** showed minimum activity(1 mm)againstProteus mirabilis and no zone of inhibition was observed againstStaphylococcus *** shrimp lethality was examined with six puffer fish *** orbicularis showed maximum mortalities as 100%*** showed minimum mortalities as 70%at a concentration of 500μg/***:In conclusion,the study showed the preliminary investigation of crude extracts of puffer fishes about the prominent activity against human bacterial *** extracts had a good cytotoxic potential against brine shrimpArtemina salina.

关键词： Puffer fishes Human pathogens Antimicrobial activity Brine shrimp lethality

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Histopathological study of lymphocystis disease virus(LCDV)in cultured false clownfish,Amphiprion ocellaris(Cuvier,1830)and true clownfish,Amphiprion percula(Lacepede,1802)

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Journal of Coastal Life Medicine 2014年第4期 264-269页

作者： muthuramalingam uthaya siva Thangapandi Marudhupandi Mohideen Abdul Badhul Haq Thipramalai Thankappan Ajith Kumar Centre of Advanced Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608502TamilnaduIndia National Bureau of Fish Genetic Resources(Indian Council of Agricultural Research) Canal Ring RoadDilkusha PostLucknow-226002Uttar PradeshIndia

Objective:To study the lymphocystis disease virus(LCDV)in two species of marine ormamental fishes through histopathological investigation along with control for differentiating the tissue ***:Six naturally infected fishes were *** were anaesthetized and subsequently killed by organ *** infected fish organs were aseptically cut off and stored with 10%formalin solution for histological *** were examined for gross pathology including location,distribution,shape,size,colour,consistency and special features of typical external lesions by standard ***:The diameter of the tumor nodules ranged from 1 to 2 mm(Amphiprion ocellaris)and 2.5 to 3.5 mm(Amphiprion percula).Light microscopic observation showed over growth of tumor like nodules on the skin or scales and ventral side as *** hy pertrophied cells with basophilic intracytoplasmic inclusion bodies were in the connective tissues of dermis and between scales on two *** nucleus of lymphocystis cell were enlarged,irregular and containing basophilic marginated ***,the similarities among cellular patterns of different fish LCDV isolates from different hosts indicated that these profiles do not depend on the host ***:In conclusion,this current study of histopathological statement of LCDV may be adequate for a presumptive diagnosis of lymphocystis disease from both marine as well as fresh water fish *** findings of asymptomatic carriers by histology using infected skin and fin sampling,which does not imply animal killing,could be important tool to epizootics caused by *** study may be very useful for further molecular studies.

关键词： Clownfish Amphiprion ocellaris Amphiprion percula Lymphocystis disease virus Histopathology

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Investigation of stingray spines by Fourier transform infrared spectroscopy analysis to recognize functional groups

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Journal of Coastal Life Medicine 2013年第3期 169-174页

作者： muthuramalingam uthaya siva Mohideen Abdul Badhul Haq Deivasigamani Selvam Ganesan Dinesh Babu Rathinam Bakyaraj Centre of Advanced Study in Marine Biology Faculty of Marine SciencesAnnamalai UniversityParangipettai-608502TamilnaduIndia

Objective:To investigate functional groups of toxic spines in stingray by Fourier transform infrared spectroscopic ***:sephen were centrifuged at 6000 r/min for 10 *** supernatant was collected and preserved separately in methanol,ethanol,chloroform,acetone(1:2)and then soaked in the mentioned solvents for 48 *** extracts were filtered and used for Fourier transform infrared spectroscopic ***:The venom extract of Himantura gerrardi,Himantura imbricata and Pastinachus and random coiled secondary *** presence of O-H stretch,C=O stretch,C-H stretch,N-H deformation,O-H deformation and C-O stretch in the sample aligned with standard bovine serum *** influence of functional groups within the molecule was because of the impact of preferred spatial orientation,chemical and physical interaction on the *** conclusion,compared to bovine serum albumin,Himantura imbricata consists of two C=O stretch,are involved in the hydrogen bonding that takes place between the different elements of secondary ***:The results identified that the presence of free amino acids and protein having β-sheet medicine not available for treatment against injuries causing ***,it's the baseline study,to motivate further process and produce effective *** venom of poisonous animals has been extensively studied,since standard.

关键词： FTIR Himantura gerrardi Himantura imbricata Pastinachus sephen Stingray spines Polypeptides Toxic spines

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