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Rickettsia influences thermotolerance in the whitefly Bemisia tabaci B biotype

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Insect Science 2011年第1期18卷 57-66页

作者： Marina Brumin Svetlana Kontsedalov murad ghanim Department of Entomology Institute of Plant Protection the Volcani Center Bet Dagan Israel

The whitefly Bemisia tabaci harbors Portiera aleyrodidarum, an obligatory symbiotic bacterium, as well as several secondary symbionts, including Rickettsia, Hamiltonella, Wolbachia, Arsenophonus, Cardinium and Fritschea, the function of which is unknown. In Israel, Rickettsia is found in both the B and Q ofB. tabaci biotypes, and while all other secondary symbionts are located in the bacteriomes, Rickettsia can occupy most of the body cavity of the insect. We tested whether Rickettsia influences the biology of B. tabaci and found that exposing a Rickettsia-containing population to increasing temperatures significantly increases its tolerance to heat shock that reached 40~C, compared to a Rickettsia-free population. This increase in tolerance to heat shock was not associated with specific induction of heat-shock protein gene expression; however, it was associated with reduction in Rickettsia numbers as was assessed by quantitative real-time polymerase chain reaction and fluorescence in situ hybridization analyses. To assess the causes for thermotolerance when Rickettsia is reduced, we tested whether its presence is associated with the induction of genes required for thermotolerance. We found that under normal 25℃ rearing temperature, genes associated with response to stress such as cytoskeleton genes are induced in the Rickettsia-containing population. Thus, the presence of Rickettsia in B. tabaci under normal conditions induces the expression of genes required for thermotolerance that under high temperatures indirectly lead to this tolerance.

关键词： cytoskeleton gene expression heat shock symbiont

Bemisia tabaci Biotype Dynamics and Resistance to Insecticides in Israel During the Years 2008-2010

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Journal of Integrative Agriculture 2012年第2期11卷 312-320页

作者： Svetlana Kontsedalov Fauzi Abu-Moch Galina Lebedev Henryk Czosnek A Rami Horowitz murad ghanim Department of Entomology Agricultural Research OrganizationVolcani CenterBet Dagan 50250Israel Institute of Plant Sciences Faculty of AgricultureRehovot 76100Israel Department of Entomology Agricultural Research OrganizationGilat Research CenterM.P.Negev 85280Israel

The sweetpotato whitefly Bemisia tabaci（Hemiptera：Aleyrodidae）is an extremely polyphagous insect pest that causes significant crop losses in Israel and *** is a species complex of which the B and Q biotypes are the most widespread and damaging *** change in biotype composition and resistance to insecticide in Israel was monitored during the years 2008-2010 to identify patterns in population dynamics that can be correlated with resistance *** results show that B biotype populations dominate crops grown in open fields,while Q biotype populations gradually dominate crops grown in protected conditions such as greenhouses and nethouses,where resistance outbreaks usually develop after several insecticide *** in previous years,Q biotype populations were widely detected in many regions in Israel,significant domination of the B biotype across populations collected was observed during the year 2010,indicating the instability of the *** population from one year to *** for the changing dynamics and the shift in the relative abundance of *** biotype,and their resistance status,are discussed.

关键词： Bemisia tabaci biotype insecticide monitoring resistance

Sequencing and comparison of the Rickettsia genomes from the whitefly Bemisia tabaci Middle East Asia Minor I

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Insect Science 2016年第4期23卷 531-542页

作者： Dan-Tong Zhu Wen-Qiang Xia Qiong Rao Shu-Sheng Liu murad ghanim Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou China Department of Entomology Agricultural Research Organization The Volcani Center Bet Dagan Israel School of Agriculture and Food Science Zhejiang Agriculture and Forestry University Lin'an Hangzhou China

The whitefly, Bemisia tabaci, harbors the primary symbiont ＇Candidatus Portiera aleyrodidarum＇ and a variety of secondary symbionts. Among these secondary symbionts, Rickettsia is the only one that can be detected both inside and outside the bacteriomes. Infection with Rickettsia has been reported to influence several aspects of the whitefly biology, such as fitness, sex ratio, virus transmission and resistance to pesticides. However, mechanisms underlying these differences remain unclear, largely due to the lack of genomic information of Rickettsia. In this study, we sequenced the genome of two Rickettsia strains isolated from the Middle East Asia Minor 1 （MEAM1） species of the B. tabaci complex in China and Israel. Both Rickettsia genomes were of high coding den- sity and AT-rich, containing more than 1000 coding sequences, much larger than that of the coexisted primary symbiont, Portiera. Moreover, the two Rickettsia strains isolated from China and Israel shared most of the genes with 100% identity and only nine genes showed sequence differences. The phylogenetic analysis using orthologs shared in the genus, inferred the proximity of Rickettsia in MEAM1 and Rickettsia bellii. Functional analysis revealed that Rickettsia was unable to synthesize amino acids required for complementing the whitefly nutrition. Besides, a type IV secretion system and a number of virulence- related genes were detected in the Rickettsia genome. The presence of virulence-related genes might benefit the symbiotic life of the bacteria, and hint on potential effects of Rickettsia on whiteflies. The genome sequences of Rickettsia provided a basis for further understanding the function of Rickettsia in whiteflies.

关键词： Bemisia tabaci comparison endosymbiont genome Rickettsia symbiosis

Green synthesis of bentonite-supported iron nanoparticles as a heterogeneous Fenton-like catalyst:Kinetics of decolorization of reactive blue 238 dye

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Water Science and Engineering 2020年第4期13卷 286-298页

作者： Ahmed Khudhair Hassan Ghayda Yaseen Al-Kindi Dalal ghanim Environment and Water Directorate Ministry of Science and TechnologyBaghdad 10070Iraq Sanitary and Environmental Branch Civil Engineering DepartmentUniversity of TechnologyBaghdad 10009Iraq

This study aimed to synthesize green tea nano zero-valent iron(GT-NZVI)and bentonite-supported green tea nano zero-valent iron(BGT-NZVI)nanoparticles using green tea extracts in an environmentally sustainable *** was used as a support material because it disperses and stabilizes GT-NZVI,and it helps to reduce the cost,increase the adsorption capacity of GT-NZVI,and decrease the optimum amount of GT-NZVI used in Fenton-like oxidation.A scanning electron microscope,an atomic force microscope,and a Fourier transform infrared spectroscope were used to characterize GT-NZVI and B-GT-NZVI,while the zeta potential was measured to evaluate the stability of iron *** decolorization kinetics of reactive blue 238(RB 238)dye in the aqueous phase in the Fenton-like oxidation process were investigated as *** effects of various experimental conditions such as reaction time,dosages of catalysts,concentration of H2O2,temperature,addition of inorganic salts,and other parameters were *** results show that the oxidative degradation efficiencies of RB 238 dye catalyzed by GT-NZVI and B-GT-NZVI were 93.5%and 96.2%,respectively,at the optimum reaction conditions as follows:c(H2O2)¼5 mmol/L,r(GT-NZVI)¼0.5 g/L or r(B-GT-NZVI)¼0.5 g/L,c(RB 238 dye)¼0.05 mmol/L,and pH¼2.5 at 180 *** best catalytic performance was exhibited when B-GT-NZVI was *** kinetic models were employed,and the second-order model was found to be the best model representing the experimental kinetic data of RB 238 *** value of activation energy decreased from 38.22 kJ/mol for GT-NZVI to 14.13 kJ/mol for *** indicates that the effect of B-GT-NZVI in decreasing the energy barrier is more pronounced than that of the GT-NZVI catalyst,leading to improved Fenton-like oxidation processes.©2020 Hohai *** and hosting by Elsevier *** is an open access article under the CC BY-NC-ND license(http://***/licenses/by-nc-nd/4.0/).

关键词： Green synthesis Bentonite-supported iron nanoparticles Azo dye Fenton-like process Kinetics

Forecasting Global Solar Insolation Using the Ensemble Kalman Filter Based Clearness Index Model

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CSEE Journal of Power and Energy Systems 2022年第4期8卷 1087-1096页

作者： Pravat Kumar Ray Bidyadhar Subudhi ghanim Putrus Mousa Marzband Zunaib Ali Department of Electrical Engineering National Institute of Technology RourkelaRourkela-769008India School of Electrical Sciences Indian Institute of Technology GoaGoa 403401India Department of Maths Physics and Electrical EngineeringNorthumbria UniversityUK

This paper describes a novel approach in developing a model for forecasting of global insolation on a horizontal *** the proposed forecasting model,constraints,such as latitude and whole precipitable water content in vertical column of that location,are *** parameters can be easily measurable with a global positioning system(GPS).The earlier model was developed by using the above datasets generated from different locations in *** model has been verified by calculating theoretical global insolation for different sites covering east,west,north,south and the central region with the measured values from the same *** model has also been validated on a region,from which data was not used during the development of the *** the model,clearness index coefficients(KT)are updated using the ensemble Kalman filter(EnKF)*** forecasting efficacies using the KT model and EnKF algorithm have also been verified by comparing two popular algorithms,namely the recursive least square(RLS)and Kalman filter(KF)*** minimum mean absolute percentage error(MAPE),mean square error(MSE)and correlation coefficient(R)value obtained in global solar insolation estimations using EnKF in one of the locations are 2.4%,0.0285 and 0.9866 respectively.

关键词： Clearness index ensemble Kalman filter extra-terrestrial irradiance forecasting global solar insolation

INITIAL COEFFICIENT ESTIMATES FOR SOME SUBCLASSES OF M-FOLD SYMMETRIC BI-UNIVALENT FUNCTIONS

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Acta Mathematica Scientia 2016年第3期36卷 863-871页

作者： H.M.SRIVASTAVA S.GABOURY F.ghanim Department of Mathematics and Statistics University of Victoria China Medical University Department of Mathematics and Computer Science University of Québec at Chicoutimi Department of Mathematics College of SciencesUniversity of Sharjah

In the present investigation, we consider two new general subclasses B∑m（T, λ; α）and B^∑m （τ λ;β） of Em consisting of analytic and m-fold symmetric bi-univalent functions in the open unit disk U. For functions belonging to the two classes introduced here, we derive non-sharp estimates on the initial coefficients [a-~＋ll and │a2＋1│. Several connections to some of the earlier known results are also pointed out.

关键词： analytic functions bi-univalent functions m-Fold symmetric functions m-Fold symmetric bi-univalent functions

Effect of pollution on DNA damage and essential fatty acid profile in Cirrhinus mrigala from River Chenab

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Chinese Journal of Oceanology and Limnology 2017年第3期35卷 572-579页

作者： Bilal HUSSAIN Tayyaba SULTANA Salma SULTANA K.A.AL-ghanim Shahid MAHBOOB Department of Zoology Government College University Department of Zoology College of Science King Saud University

The objective of this study was to evaluate the effect of anthropogenic pollution on DNA damage and the fatty acid profile of the bottom dweller fish （Cirrhinus mr^gala）, collected from the River Chenab, in order to assess the effect of the toxicants on the quality of the fish meat. The levels ofCd, Hg, Cu, Mn, Zn, Pb, Cr and Sn and of phenols from this river were significantly higher than the permissible limits set by the USEPA. Comet assays showed DNA damage in Cirrhinus mr^gala collected from three different sampling sites in the polluted area of the river. Significant differences were observed for DNA damage through comet assay in fish collected from polluted compared to control sites. No significant differences were observed for DNA damage between farmed and fish collected from upstream. The micronucleus assay showed similar trends. Fish from the highly polluted sites showed less number of fatty acids and more saturated fatty acids in their meat compared to fish from less polluted areas. Several fatty acids were missing in fish with higher levels of DNA in comet tail and micronucleus induction. Long-chain polyunsaturated fatty acids, eicosapentaenoic acid （20：5n-3） was found missing in the fish from polluted environment while it was found in considerable amount in farmed fish 7.8±0.4%. Docosahexaenoic acid （22：6n-3） also showed significant differences as 0.1±0.0 and 7.0±0.1% respectively, in wild polluted and fanned fishes.

关键词： habitat fish comet assay micronucleus assay fatty acids

Glucagon-like peptide-1 receptor agonists as a possible intervention to delay the onset of type 1 diabetes:A new horizon

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World Journal of Diabetes 2024年第2期15卷 133-136页

作者： Mahmoud Nassar Ajay Chaudhuri Husam ghanim Paresh Dandona Department of Internal Medicine Division of EndocrinologyDiabetes and MetabolismJacobs School of Medicine and Biomedical SciencesUniversity of BuffaloBuffaloNY 14221United States

Type 1 diabetes(T1D)is a chronic autoimmune condition that destroys insulinproducing beta cells in the pancreas,leading to insulin deficiency and *** management of T1D primarily focuses on exogenous insulin replacement to control blood glucose ***,this approach does not address the underlying autoimmune process or prevent the progressive loss of beta *** research has explored the potential of glucagon-like peptide-1 receptor agonists(GLP-1RAs)as a novel intervention to modify the disease course and delay the onset of ***-1RAs are medications initially developed for treating type 2 *** exert their effects by enhancing glucose-dependent insulin secretion,suppressing glucagon secretion,and slowing gastric *** evidence suggests that GLP-1RAs may also benefit the treatment of newly diagnosed patients with *** article aims to highlight the potential of GLP-1RAs as an intervention to delay the onset of T1D,possibly through their potential immunomodulatory and anti-inflammatory effects and preservation of *** article aims to explore the potential of shifting the paradigm of T1D management from reactive insulin replacement to proactive disease modification,which should open new avenues for preventing and treating T1D,improving the quality of life and long-term outcomes for individuals at risk of T1D.

关键词： Type 1 diabetes Semaglutide Glucagon-like peptide-1 receptor agonists Insulin therapy Autoimmune response Blood glucose monitoring Β-cell preservation Early screening Teplizumab Randomized controlled trials

Cobalt Sulfide Confined in N-Doped Porous Branched Carbon Nanotubes for Lithium-Ion Batteries

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Nano-Micro Letters 2019年第2期11卷 157-165页

作者： Yongsheng Zhou Yingchun Zhu Bingshe Xu Xueji Zhang Khalid A.Al.ghanim Shahid Mahboob College of Chemistry and Materials Engineering Anhui Science and Technology UniversityBengbu 233030People’s Republic of China Key Laboratory of Inorganic Coating Materials CAS Shanghai Institute of CeramicsChinese Academy of SciencesShanghai 200050People’s Republic of China Key Laboratory of Interface Science and Engineering in Advanced Materials Ministry of EducationTaiyuan University of TechnologyTaiyuan 030024People’s Republic of China Beijing Key Laboratory for Bioengineering and Sensing Technology Research Center for Bioengineering and Sensing TechnologySchool of Chemistry and BioengineeringUniversity of Science and Technology Beijing30 Xueyuan RoadHaidian DistrictBeijing 100083People’s Republic of China Department of Zoology College of ScienceKing Saud UniversityRiyadhSaudi Arabia Department of Zoology GC UniversityFaisalabadPakistan

Lithium-ion batteries(LIBs) are considered new generation of large-scale energy-storage ***,LIBs suffer from a lack of desirable anode materials with excellent specific capacity and cycling *** this work,we design a novel hierarchical structure constructed by encapsulating cobalt sulfide nanowires within nitrogen-doped porous branched carbon nanotubes(NBNTs)for *** unique hierarchical Co9S8@NBNT electrode displayed a reversible specific capacity of 1310 mAhg-1 at a current density of 0.1 Ag-1,and was able to maintain a stable reversible discharge capacity of 1109 mAhg-1 at a current density of 0.5 Ag-1 with coulombic efficiency reaching almost 100% for 200 *** excellent rate and cycling capabilities can be ascribed to the hierarchical porosity of the one-dimensional Co9S8@NBNT internetworks,the incorporation of nitrogen doping,and the carbon nanotube confinement of the active cobalt sulfide nanowires offering a proximate electron pathway for the isolated nanoparticles and shielding of the cobalt sulfide nanowires from pulverization over long cycling periods.

关键词： Lithium-ion batteries Nitrogen doping Cobalt sulfide Branched carbon nanotubes