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A Novel System for Xylem Cell Differentiation in Arabidopsis thaliana

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Molecular Plant 2015年第4期8卷 612-621页

作者： Yuki Kondo Takashi Fujita munetaka Sugiyama Hiroo Fukuda Department of Biological Sciences Graduate School of Science The University of Tokyo 7-3-1 Hongo Bunkyo-ku tokyo 113-0033 Japan Botanical Gardens Graduate School of Science Tile University of Tokyo 3-7-1 Hakusan Bunkyo-ku Tokyo 112-0001 Japan

During vascular development, procambial and cambial cells give rise to xylem and phloem cells. Because the vascular tissue is deeply embedded, it has been difficult to analyze the processes of vascular development in detail. Here, we establish a novel in vitro experimental system in which vascular development is induced in Arabidopsis thaliana leaf-disk cultures using bikinin, an inhibitor of glycogen synthase kinase 3 proteins. Transcriptome analysis reveals that mesophyll cells in leaf disks synchronously turn into procambial cells and then differentiate into tracheary elements. Leaf-disk cultures from plants expressing the procambial cell markers TDRpro：GUS and TDRpro：YFP can be used for spatiotemporal visualization of procambial cell formation. Further analysis with the tdr mutant and TDIF （tracheary element differentiation inhibitory factor） indicates that the key signaling TDIF-TDR-GSK3s regulates xylem differentiation in leaf-disk cultures. This new culture system can be combined with analysis using the rich material resources for Arabidopsis including cell-marker lines and mutants, thus offering a powerful tool for analyzing xylem cell differentiation.

关键词： Arabidopsis thaliana bikinin leaf-disk culture transcriptome tracheary element differentiation signaling

The Impact of Footwear on Posture, Gait and Balance

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Health 2022年第2期14卷 209-218页

作者： Saori Karasawa munetaka Yamamoto Junko Sakurai Shiori Kawasaki Hiroyuki Kobayashi Department of Hospital Administration Juntendo Graduate School of Medicine Tokyo Japan Patient Safety Management Section Juntendo University Hospital Tokyo Japan Faculty of Health and Nursing Juntendo University Chiba Japan

Background: The objective of this study was to investigate the effects of footwear on posture and balance while walking. The types of footwear investigated in this study were open back shoes, commonly worn by hospitalized patients, and closed back shoes. Previous studies have shown that open back shoes, or slippers, increase the risk of falling (among elderly). We hypothesized that our findings would suggest that open back shoes negatively affect gait mechanics in healthy individuals. Methods: Healthy individuals (n = 12) participated in a walking test while wearing closed back shoes and open back shoes. The explanatory variables in this study were the analysis of gait, posture, and balance before and after walking. The objective variable was footwear (closed back shoes vs. open back shoes). A paired t-test was performed to detect significant differences between the two conditions. Results: Among the test items measured, we found a significant difference in minimum wide tilt angle and left-right differences in step length and intensity while walking between the conditions of closed back shoes and open back shoes. These results suggest that open back shoes could negatively impact posture and balance while walking, even in healthy subjects. Conclusion: It is imperative to improve patient awareness of the risk of falling. We believe that the inclusion of our findings in educational pamphlets and in-house notices could help improve patient awareness and more effectively prevent falls among patients.

关键词： Footwear Posture Walking Test Health Fall

Separation, Recovery, and Recycling MSW with Steam Classification

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Separation, Recovery, and Recycling MSW with Steam Classific...

Bacterial Infection in the Limbs of Patients with Rheumatoid Arthritis during Biological Agent Therapy

7th World Congress on Recovery, Recycling and Re-integration

作者： munetaka Ikeda Global Business Navigation Incorporation 2185 Station Village Way San Diego California 92108 United States of America

Steam Classification is a process for the treatment of commingled municipal solid waste (MSW) with steam under pressure and agitation to transform certain waste components, such as packaging materials, paper products, food waste, into a homogenous cellulosic biomass product. Initial volume reduction of the processed MSW is 50%-70%. The biomass product may be used directly for energy production as combustion or gasification fuel. The biomass may also be further processed to produce clean fiber suitable for recycling into a variety pulp and paper products. The biomass may also be used in the production of medium density fiberboard, plastic lumber, cement and concrete products, asphalt paving, and chemicals. In addition to the biomass, clean recyclable steel and aluminum cans are recovered from the process free of food, labels, and laminates. Recyclable PET plastic bottles are flattened, shrunken, and freed of labels during processing HOPE, LDPE and other plastics are melted during processing and recovered as agglomerates or films suitable for making plastic lumber and pipe. Broken glass, ceramics, rock, ash and dirt may also be recovered and recycled into aggregate for cement, concrete, asphalt and plastic pipe. Textiles may also be recovered and recycled. Other components, generally sterile and inert, may be safely landfilled. The first 500 t/d facility will be in operation in Anaheim, California in January 2005. The patented process has been licensed to World Waste Technologies, Inc., San Diego, California for development in the U.S.A. (***), The Fernwood Group, Ltd., Nottingham, U.K. for development in Europe, and International Waste Processors Inc., San Diego, California for development in the Middle East and in cooperation with Global Business Navigation, Inc., San Diego, California for Asia.

关键词： municipal solid waste, steam classification, recycling, cellulose, biomass

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Open Journal of Rheumatology and Autoimmune Diseases 2012年第3期2卷 47-52页

作者： Hajime Yamanaka Kenichiro Goto munetaka Suzuki Department of Orthopaedic Surgery National Hospital Organization Shimoshizu Hospital Yotsukaido City Japan

Biological therapies in rheumatoid arthritis (RA) are known to increase the risk of serious infections. The present study was performed to evaluate the clinical features of bacterial infections occurring in the limbs during biological therapies in patients with RA. By March 2011, 11 RA patients (14 limbs) treated with biological agents at our institution required hospitalization due to bacterial infections occurring in the limbs. These patients had an average age of 53.7 years old. Infections occurred an average of 19 months after biological treatment. Two limbs in one patient were treated with infliximab, eight limbs in six patients were treated with etanercept, one limb in one patient was treated with adalimumab, and three limbs in three patients were treated with tocilizumab. Cellulitis occurred in 7 limbs, late infections after total knee arthroplasty occurred in two limbs, early infections after orthopedic surgery occurred in three limbs, and septic arthritis occurred in two limbs. Four cases had comorbidities—liver cirrhosis and diabetes mellitus in one and three cases, respectively. All patients were treated using corticosteroid with an average dose of 4.6 mg daily. Seven limbs required surgical treatment. All patients finally recovered. Ten limbs continued treatment with biological agents. Care must be taken regarding bacterial infection in the limbs of RA patients treated by using biological agents, particularly those with comorbidities. Further studies are required to confirm means of preventing such infections in daily practice.

关键词： Bacterial Infection Limbs Rheumatoid Arthritis Biological Agents

Electrochemical Characteristics of Indium Tin Oxide Electrodes Modified with Gold Nanoparticles Using a Novel Attachement Approach

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Electrochemical Characteristics of Indium Tin Oxide Electrod...

Novel Method to Attach Gold Nanoparticles on Indium Tin Oxide Surface

2～(nd) Spring Meeting of the International Society of Electrochemistry

作者： Miyako Kambayashi munetaka Oyama Division of Research Initiatives International Innovation Center Kyoto University Sakyo-ku Kyoto 606-8501 Japan Division of Research Initiatives International Innovation Center Kyoto University Sakyo-ku Kyoto 606-8501 Japan

Nanomaterials have unique physical and chemical properties that are not only highly size-dependent but also strongly shape-related. Various nanomaterials such as metal andsemiconductor nanoparticles and carbon nanotubes have been prepared and developed aselectrode materials and their electrochemical characteristics have been extensively introduced.

关键词： MPTMS ITO Electrochemical Characteristics of Indium Tin Oxide Electrodes Modified with Gold Nanoparticles Using a Novel Attachement Approach

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Novel Method to Attach Gold Nanoparticles on Indium Tin Oxid...

新辅助化疗后行腹腔镜辅助胃切除术治疗晚期胃癌

2～(nd) Spring Meeting of the International Society of Electrochemistry

Metal nanoparticles have unique optical, electronic, magnetic, and catalytic properties that areoften different from those of the bulk metals. These properties are currently under intensestudy for potential uses as optoelectronic components, spectroscopic enhancers, biologicaldetection labels, and chemical sensing elements. For electrochemical measurements, muchattention has been devoted to the modification of electrodes with metal nanoparticles, whichis a promising approach to provide nano-structured functional interfaces.

关键词： Novel Method to Attach Gold Nanoparticles on Indium Tin Oxide Surface ITO

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中国普通外科杂志 2013年第10期22卷 1238-1242页

作者： Takaki Yoshikawa Takashi Oshima Yasushi Rino munetaka Masuda 日本横滨神奈川癌症中心胃肠外科日本横滨市立大学医学院外科

目前治疗晚期胃癌的方法为开腹胃切除及D2淋巴结清扫并在术后行S-1或卡倍他滨加奥沙利铂辅助化疗。然而III期胃癌患者的预后并不理想。为了提高生存率,需要加大化疗药物的剂量。鉴于患者接受二联或三联化疗的顺应性差,新辅助化疗将是一... 详细信息

目前治疗晚期胃癌的方法为开腹胃切除及D2淋巴结清扫并在术后行S-1或卡倍他滨加奥沙利铂辅助化疗。然而III期胃癌患者的预后并不理想。为了提高生存率,需要加大化疗药物的剂量。鉴于患者接受二联或三联化疗的顺应性差,新辅助化疗将是一个充满前景的方法。目前在东亚国家,正进行多项III期临床试验用于评估新辅助化疗效果。另一方面,手术治疗方法已倾向于行腹腔镜手术。在日本和韩国已经进行了多项III期临床试验评估腹腔镜辅助远端胃切除(LADG)在早期和晚期患者中的疗效。因此,未来标准治疗肿瘤位于中、下1/3晚期胃癌的候选方案由综合治疗组成,包括新辅助化疗及随后的LADG。只要新辅助化疗是按标准实施,随后的腹腔镜胃切除术的可行性、安全性及长期生存率是有保障的。在这个背景下,我们实施了一项随机II期临床试验比较新辅助化疗后LADG和开腹远端胃切除术(ODG)治疗胃癌的疗效。

关键词：胃肿瘤腹腔镜胃切除术新辅助化疗

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一种基于碳纳米管的色氨酸传感器

应用化学 2007年第12期24卷 1433-1438页

作者：郑燕琼杨昌柱张敬东 munetaka Oyamab 濮文虹龙峰华中科技大学环境科学与工程学院武汉430074 Division of Research Initiatives International Innovation CenterKyoto UniversityKyotoJapan

研究了色氨酸（tryptophan即Trp）在多壁碳纳米管修饰玻碳电极（MWNTs／GC）上的电化学行为。MWNTs／GC电极对Trp具有良好的电催化作用,相对于GC电极，Trp在MWNTs／GC上峰电位负移128mV，峰电流约为Gc电极上氧化峰电流的31倍。在1．0mol... 详细信息

研究了色氨酸（tryptophan即Trp）在多壁碳纳米管修饰玻碳电极（MWNTs／GC）上的电化学行为。MWNTs／GC电极对Trp具有良好的电催化作用,相对于GC电极，Trp在MWNTs／GC上峰电位负移128mV，峰电流约为Gc电极上氧化峰电流的31倍。在1．0mol／LH2SO4中清洗能更新电极表面，消除产物吸附带来的影响。MWNTs／GC电极在含1．0×10^-4mol／L Trp的缓冲溶液中闭路富集2min时电流达到稳定值。研究了不同pH值影响的结果表明，参与电极反应的质子数和电子数相等。在pH=2．2时，Trp的氧化电流最大。利用LSV研究了电流与扫描速率的关系，结果表明，Trp在修饰电极上的氧化过程为扩散控制过程。在环境温度低于45℃时，随着温度增加，氧化电流逐渐增大。温度在16-35℃范围内,传感器的响应电流与温度成线性关系，温度系数为0．695μA／℃，说明此传感器在实测过程中因温度波动带来的测量误差很小。利用LSV研究了氧化峰电流与Trp的浓度关系的结果显示，峰电流与Trp的浓度在1．00×10^-6—1．00×10^-4moL／L范围内呈良好线性关系，检出限为1．82×10^-7mol／L（S／N=3）。该电极具有良好的灵敏度、选择性和稳定性,放置7d后，碳纳米管的峰电流仍能达到最初电流的98％。

关键词：色氨酸传感器多壁碳纳米管修饰电极

纳米铂直接修饰玻碳电极的制备及在半胱氨酸中的电化学行为

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应用化学 2007年第8期24卷 868-872页

作者：濮文虹黄金桃钱功明张敬东 munetaka Oyama 桂娟杨昌柱华中科技大学环境科学与工程学院武汉430074 Division of Research Initiatives International Innovation CenterKyoto UniversitySakyo-kuKyoto 606-8501Japan

采用一步化学原位还原法将球形纳米铂颗粒直接修饰在玻碳电极上,用SEM、EDS和电化学方法对该电极进行表征并与铂片电极、裸玻碳电极进行了对比。结果表明,纳米铂修饰电极的峰电流与扫描速度呈线性关系,纳米铂在电极表面覆盖率为1.28×10... 详细信息

采用一步化学原位还原法将球形纳米铂颗粒直接修饰在玻碳电极上,用SEM、EDS和电化学方法对该电极进行表征并与铂片电极、裸玻碳电极进行了对比。结果表明,纳米铂修饰电极的峰电流与扫描速度呈线性关系,纳米铂在电极表面覆盖率为1.28×10-7mol/cm2。循环伏安法研究结果表明纳米铂修饰电极对半胱氨酸的催化氧化作用和铂片电极相比提高了数倍,且峰电位负移了0.3V。在纳米铂修饰的玻碳电极上,半胱氨酸的浓度在1.0×10-7mol/L到1.0×10-5mol/L范围内和催化电流呈线性关系。

关键词：化学原位一步还原法纳米铂半胱氨酸玻碳