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In Vitro Antioxidant and Radio Protective Activities of Lycopene from Tomato Extract against Radiation—Induced DNA Aberration

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Journal of Biosciences and Medicines 2024年第2期12卷 202-213页

作者： Safaiatul Islam Abu hena Mostofa Kamal Md. Ziaur Rahman Protul Kumar Roy A.Y.K. Md. Masud Rana Molecular Radiobiology and Biodosimetry Division Institute of Food and Radiation Biology Bangladesh Atomic Energy Commission Savar Bangladesh

Background: The accumulation of free radicals is linked to a number of diseases. Free radicals can be scavenged by antioxidants and reduce their harmful effects. It is therefore essential to look for naturally occurring antioxidants that come from plants, as synthetic antioxidants are toxic, carcinogenic and problematic for the environment. Lycopene is one of the carotenoids, a pigment that dissolves in fat and has antioxidant properties. Materials and Methods: The antioxidant and free radical scavenging activity were assessed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The impact of lycopene on bacteria (E. coli) susceptibility to γ-radiation was examined by radio sensitivity assay. The study also examined the induction of strand breaks in plasmid pUC19 DNA and how lycopene extract protected the DNA from γ-radiation in vitro. Results: At varying concentrations, lycopene demonstrated its ability to scavenge free radicals such as 2, 2-diphenyl-1-picrylhydrazyl (DPPH). IC50 for lycopene was determined at 112 μg/mL which was almost partial to IC50 of standard antioxidant L-ascorbic acid. The D10 value 180 Gy of E. coli was found to be >2-fold higher in the extract-containing lycopene sample than in the extract-free controls. The lycopene extracts inhibited the radiation-induced deterioration of the plasmid pUC19 DNA. At an IC50 concentration, lycopene provided the highest level of protection. Conclusion: Lycopene functions as an efficient free radical scavenger and possible natural antioxidant source. For cancer patients and others who frequently expose themselves to radiation, lycopene may be a useful plant-based pharmaceutical product for treating a variety of diseases caused by free radicals.

关键词： Radio Protective Antioxidants Free Radical DNA Damage pUC19 Plasmid Gamma Irradiation DPPH

Rainfall Sensitivity to Microphysics and Planetary Boundary Layer Parameterizations in Convection-Permitting Simulations over Northwestern South America

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Journal of Meteorological Research 2024年第4期38卷 805-825页

作者： K.Santiago HERNANDEZ Sebastian GOMEZ-RIOS Juan J.henaO Vanessa ROBLEDO Alvaro RAMiREZ-CARDONA Angela M.RENDON Geolimna Escuela AmbientalFacultad de IngenieriaUniversidad de AntioquiaMedellin 050010Colombia Grupo de Ingenieria y Gestion Ambiental(GIGA) Escuela AmbientalFacultad de IngenieriaUniversidad de AntioquiaMedellin 050010Colombia Division of Atmospheric Sciences Desert Research InstituteRenoNV 89512USA

Convection-permitting modeling allows us to understand mechanisms that influence rainfall in specific ***,microphysics parameterization(MP) and planetary boundary layer(PBL) schemes remain an important source of uncertainty,affecting rainfall intensity,occurrence,duration,and ***,we study the sensitivity of rainfall to three MP [Weather Research and Forecasting(WRF) Single-Moment 6-class(WSM6),Thompson,and Morrison] and two PBL [the Yonsei University(YSU) and Mellor–Yamada Nakanishi Niino(MYNN)] schemes with a convection-permitting resolution(4 km) over northwestern South America(NWSA).Simulations were performed by using the WRF model and the results were evaluated against soundings,rain gauges,and satellite data,considering the spatio-temporal variability of rainfall over diverse regions prone to deep convection in *** and PBL schemes largely influenced simulated rainfall,with better results for the less computationally expensive WSM6 MP and YSU PBL *** rain gauges and satellite estimates,simulations with Morrison MP overestimated rainfall,especially westward of the Andes,whereas the MYNN PBL underestimated precipitation in the Amazon–Savannas *** found that the uncertainty in the rainfall representation is highly dependent on the region,with a higher influence of MP in the Colombian Pacific and PBL in the Amazon–Savannas *** analyzing rainfall-related processes,the selection of both MP and PBL parameterizations exerted a large influence on the simulated lower tropospheric moisture flux and moisture *** schemes significantly influenced the downward shortwave radiation,with MYNN simulating a greater amount of low clouds,which decreased the radiation ***,latent heat fluxes were greater for YSU,favoring moist convection and *** schemes had a marked impact on vertical ***,Morrison MP showed stronger convection and higher precipitation rates,which is assoc

关键词： sensitivity microphysics planetary boundary layer(PBL) Weather Research and Forecasting(WRF) processes

Seasonal changes in Porteresia coarctata (Tateoka) beds along a subtropical coast

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Chinese Journal of Oceanology and Limnology 2013年第1期31卷 1-9页

作者： ABU hena M K Department of Animal Science and Fishery Faculty of Agriculture and Food Sciences University Putra Malaysia Bintulu Sarawak Campus

Shoot density, standing crop （above- and below-ground biomass） and habitat of salt marsh grass Porteresia coarctata were investigated along the coast of Bakkhali estuary, Cox＇s Bazar, Bangladesh from January to December 2006. Shoot density of P. coarctata was influenced by season and was found to be higher （〉2 500 shoots/m2） in post-monsoon and minimal in monsoon season; plants were particularly active in vegetative propagation during pre-monsoon. Above-ground biomass was greater along the protected coast compared with the exposed one in this estuary. Below-ground biomass was higher （7.75-269.53 g DW/m2） than that above ground （2.20-114.75 g DW/m2）. Standing crops ofP. coarctata showed a negative relationship （R=-0.77; P〈0.05） with sedimentation rate, while seasonal activity influenced sedimentation. The recorded sedimentation rate was lower （6.09 mg/（cm2·d）） in pre-monsoon and highest （14.55 mg/（cm2·d）） in monsoon season. The mean value of pore water salinity was higher （34.25±65.05） during post-monsoon and lowest （18.0±3.71） in monsoon season. The soil was sandy clay in this P. coarctata bed; it consisted of 86% sand, 13% clay and 1% silt. Soil organic matter dropped during the monsoon season （0.78%-0.67%） and was highest （（2.17±1.42）%-（2.3±1.47）%） during post-monsoon, probably owing to accumulation of decomposed peat on the marsh surface. The mean pore water NH4-N concentration ranged from 2.444-1.65 to 3.33±1.82 μg/L, with a minimum air temperature of 22.09℃ in post-monsoon and a maximum of 31.16℃ in pre-monsoon. Variations of physico-chemical parameters in the soil, water, and climate governed biological parameters of P. coarctata in the Bakkhali estuary, and were comparable with estuarine environments elsewhere.

关键词： standing crop salt marsh Porteresia coarctata Bangladesh

Co-hydrotreating light cycle oil-canola oil blends

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Frontiers of Chemical Science and Engineering 2015年第1期9卷 64-76页

作者： Huafi WANG hena FAROOQI Jinwen CHEN Natural Resources Canada CanmetENERGY-Devon One Oil Patch Drive Devon AB T9G1A8 Canada

Canola oil and light cycle oil （LCO） blends were co-hydrotreated over a commercial hydrotreating catalyst （NiMo/Al2O3） to produce diesel fuel with a green diesel component. High hydrodeoxygenation, hydrodesul- phurization and hydrodenitrogenation catalytic activities were achieved for all three feedstocks tested in the temperature range of 350-380℃ with a hydrogen pressure of 7 MPa and a gas/oil ratio of 800 nL/L. The hydrocrack- ing conversion of the 360℃ ＋ materials in the feedstocks increased by 5% and 15% when 5 and 7.5 wt-% canola oil was added to the LCO, respectively. Compared to the pure LCO, the cetane index of the diesel product formed from hydrotreating the two canola oil-LCO blends increased by 2.5 and 4, respectively. Due to the higher hydrogen requirement to crack and deoxygenate the triglycerides contained in the canola oil, a higher hydrogen consumption was needed to hydrotreat the canola oil-LCO blends.

关键词： hydrotreating co-hydrotreating co-proces-sing canola oil light cycle oil (LCO)

The mechanism of a new type of modified clay controlling Phaeocystis globosa growth

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Journal of Oceanology and Limnology 2020年第4期38卷 1270-1282页

作者： QIU Lixia YU Zhiming CAO Xihua JI hena SONG Xiuxian CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Phaeocystis globosa is a harmful algal bloom(HAB)species *** modified clay(MC)to control HABs and to mitigate their adverse effects is currently a commonly used method in *** this paper,the effects of oxidized composite modified clay(OXI-MC)on *** were studied from different *** results show that the OXI-MC could effectively remove *** and inhibit both the growth of residual algal cells and the formation of new *** *** algal biomass removal efficiencies after 3 h reached 90%at a dose of 0.1 g/L,and the number of colonies with different particle sizes was greatly *** with those of the control,the superoxide dismutase(SOD)activity,catalase(CAT)activity,and malondialdehyde(MDA)content of the residual algae significantly increased,indicating that OXI-MC caused oxidative stress in the algal *** addition,we evaluated the effects of OXI-MC on the photosynthesis of residual microalgae and found that the maximal photochemical efficiency of photosystem II(PSII)under dark adaptation(F v/F m)and actual photochemical efficiency of PSII(ФPSII)decreased,severely damaging the photosynthesis efficiency,implying that OXI-MC effected the photosynthesis system of *** results of this study clarify that OXI-MC could remove the most of algal cells and break up the colonies of *** by collision,flocculation,and releasing active substances,as well as inhibit effectively the growth and colony formation of residual *** by causing oxidative stress,reducing photosynthesis activity,accelerating the degradation of polysaccharides,and inhibiting the formation of colonies.

关键词： Phaeocystis globosa modified clay colony disruption oxidative stress photosynthesis

Programmed cell death induced by modified clay in controlling Prorocentrum donghaiense bloom

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Journal of Environmental Sciences 2021年第11期33卷 123-134页

作者： hena Ji Zhiming Yu Liyan He Jianan Zhu Xihua Cao Xiuxian Song CAS Key Laboratory of Marine Ecology and Environmental Sciences Institute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory of Marine Ecology and Environmental Science Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Modified clay(MC),an effective material used for the emergency elimination of algal blooms,can rapidly reduce the biomass of harmful algal blooms(HABs)via *** that,MC can still control bloom population through indirect effects such as oxidative stress,whichwas initially proposed to be related to programmedcell death(PCD)at molecular *** further study theMC induced cell death in residual bloom organisms,especially identifying PCD process,we studied the physiological state of the residual Prorocentrum *** experimental results showed that flocculation changed the physiological state of the residual cells,as evidenced by growth inhibition and increased reactive oxygen species ***,this research provides biochemical and ultrastructural evidence showing that MC induces PCD in *** changes were observed,and increased caspase-like activity,externalization of phosphatidylserine and DNA fragmentation were detected in MC-treated groups and *** the mitochondrial apoptosis pathway was activated in both MC-treated ***,the features of MC-induced PCD in a unicellular organism were summarized and its concentration dependent manner was *** our preliminary results elucidate the mechanism through which MC can further control HABs by inducing PCD and suggest a promising application of PCD in bloom control.

关键词： Modified clay Prorocentrum donghaiense Programmed cell death Harmful algal blooms

面向居民家庭智慧用能的随器量测模块设计与应用

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现代电子技术 2024年第13期47卷 180-186页

作者：陈贺娜郝伟琦曹波康守信王丙休王贤辉北京智芯微电子科技有限公司北京102200 北京智芯半导体科技有限公司北京102299

针对低压居民用户用能负荷感知、测量、采集和调控需求,文中研究了面向居民家庭智慧用能的随器量测模块设计与应用技术。首先,分析了居民家庭智慧用能双向互动的业务场景;接着,进行了随器量测模块的硬件和软件设计,通过搭载高精度计量... 详细信息

针对低压居民用户用能负荷感知、测量、采集和调控需求,文中研究了面向居民家庭智慧用能的随器量测模块设计与应用技术。首先,分析了居民家庭智慧用能双向互动的业务场景;接着,进行了随器量测模块的硬件和软件设计,通过搭载高精度计量芯片、HPLC+HRF双模通信芯片和内嵌随器量测软件协议栈,实现了用电设备的嵌入式计量、通信、控制等主要功能。所研制随器量测模块经第三方机构检测,计量精度达到1级,采样频率优于20 kHz,满足用电设备高效双向通信需求。该技术在河北开展了智慧用能示范应用,实现了居民负荷电器级深度感知和精准调节,得到工程实践的验证。

关键词：智慧用能随器量测用电设备负荷调节 HPLC+HRF双模通信芯片深度感知

Radiographic evaluation of vascularity in scaphoid nonunions:A review

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World Journal of Orthopedics 2020年第11期11卷 475-482页

作者： hena S Cheema Adnan N Cheema Department of Diagnostic Radiology University of PennsylvaniaPhiladelphiaPA 19104United States Department of Orthopaedic Surgery University of PennsylvaniaPhiladelphiaPennsylvania 19104United States

Scaphoid fractures,particularly those that occur more proximally,are unreliable in achieving union due to the retrograde blood supply of the scaphoid *** compromise is associated with the development of nonunions and avascular necrosis of the proximal *** to the tenuous blood supply of the scaphoid,it is imperative that the vascularity be assessed when creating diagnostic and treatment *** detection of vascular compromise via imaging may signal impending nonunion and allow clinicians to perform interventions that aid in restoring perfusion to the *** compromise in the scaphoid presents a diagnostic challenge,in part due to the non-specific findings on plain radiographs and computed *** resonance imaging techniques have dramatically improved our ability to assess the blood supply to the scaphoid and improve time to *** review aims to summarize these advances and highlights the importance of imaging in assessing vascular compromise in scaphoid nonunion and in reperfusion following surgical intervention.

关键词： Vascularity Perfusion Scaphoid fracture Scaphoid nonunion Scaphoid open reduction and internal fixation Bone graft

Bacteriocin from Bacillus subtilis as a novel drug against diabetic foot ulcer bacterial pathogens

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Asian Pacific Journal of Tropical Biomedicine 2013年第12期3卷 942-946页

作者： Baby Joseph Berlina Dhas Vimalin hena Justin Raj Interdisciplinary Research Centre Department of BiotechnologyMalankara Catholic College Department of Microbiology Hindusthan College of Arts and Science

Objective:To isolate and identify Bacillus subtilis(***)from soil and to characterize and partially purify the *** evaluate the antimicrobial activity against four diabetic foot ulcer bacterial ***:Genotypic identification was done based on Sergey's manual of systemic *** susceptibility test was done by Kirby-Bauer disc diffusion *** were identified by colony morphology and biochemical characterization and also compared with MTCC 121 *** identification was done by 16S rRNA *** activities of partially purified bacteriocin on all the DFU isolates were done by agar well diffusion *** strain was identified to produce bacteriocin by stab overlay *** was extracted by organic solvent extraction using chloroform,further purified by HPI.C and physical,and chemical characterization was ***:The four isolates showed high level of resistance to amoxyclav and sensitivity to *** purification revealed that the extracts are *** phylogenetic tree analysis results showed that the isolate was 99%related to *** *** results reveled activity to all the four isolates and high level of activity was seen in case of Klebsiella ***:Partially purified bacteriocin was found to have antimicrobial activity against the four diabetic foot ulcer bacterial pathogens,which can thus be applied as a better drug molecule on further *** strain *** are found to be safe for use and these antimicrobial peptides can be used as an antimicrobial in humans to treat DFU bacterial pathogens.

关键词： 16S rRNA Antimicrobial activity Micrococcus leuteus HPLC Physicochemical characterization