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Long-term aspirin pretreatment in the prevention of cerulein-induced acute pancreatitis in rats

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World Journal of Gastroenterology 2013年第19期19卷 2894-2903页

作者： Ibrahim Akyazi Evren Eraslan Ahmet Gülubuk Elif Ergül Ekiz Zeynep L rakli Damla Haktanir Deniz Aktaran Bala Mete zkurt Erdal Matur mukaddes zcan Department of Physiology Veterinary FacultyIstanbul University34320 IstanbulTurkey Department of Pathology Veterinary FacultyIstanbul University34320 IstanbulTurkey Department of Biochemistry Bakirkoy Dr.Sadi Konuk Training and Research Hospital34147 IstanbulTurkey Department of Physiology Faculty of MedicineEski ehir Osmangazi University26480 EskisehirTurkey

AIM:To investigate the effects of long term pretreatment with low-,medium-and high-dose aspirin(acetylsalicylic acid,ASA) on a model of acute pancreatitis(AP) induced in ***:Forty male Wistar rats were *** experimental groups,each consisting of eight animals,received low-(5 mg/kg per day),medium-(150 mg/kg per day) and high-dose(350 mg/kg per day) ASA in supplemented pellet chow for 100 *** animals,serving as the AP-control group,and another eight,serving as reference value(RV) group,were fed with standard pellet chow for the same *** pretreatment,AP was induced in the experimental animals by intraperitoneal administration of cerulein(2 × 50 μg/kg),while the RV group received saline in the same *** hours after the second injection,the animals were *** tissue and plasma samples were *** part of the collected pancreatic tissues was used for histopathological evaluation,and the remaining portion was *** levels [tumor necrosis factor,interleukin(IL)1β,IL-6],hemogram parameters,biochemical parameters(amylase and lipase),nuclear factor-κB,aspirin triggered lipoxins and parameters related to the antioxidant system(malondialdehyde,nitric oxide,hemeoxygenase-1,catalase and superoxide dismutase) were ***:Cerulein administration induced mild pancreatitis,characterized by interstitial edema(total histopathological score of 5.88 ± 0.44vs 0.25 ± 0.16,P < 0.001).Subsequent pancreatic tissue damage resulted in an increase in amylase(2829.71 ± 772.48 vs 984.57 ± 49.22 U/L,P = 0.001) and lipase(110.14 ± 75.84 U/L vs 4.71 ± 0.78 U/L,P < 0.001) in plasma,and leucocytes(6.89 ± 0.48 vs 4.36 ± 0.23,P = 0.001) in peripheral ***,IL-1β(18.81 ± 2.55 pg/μg vs 6.65 ± 0.24 pg/μg,P = 0.002) and IL-6(14.62 ± 1.98 pg/μg vs 9.09 ± 1.36 pg/μg,P = 0.04) in pancreatic tissue also *** pretreatment reduced the increase in the aforementioned parameters to a certain degree and partially i

关键词： Aspirin Acute pancreatitis Cerulein Antioxidant Cytokines

Experimental and clinical evidence of antioxidant therapy in acute pancreatitis

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World Journal of Gastroenterology 2012年第39期18卷 5533-5541页

作者： mukaddes Esrefoglu Department of Histology and Embryology Bezmialem Vakif University Medical Faculty34093 FatihIstanbulTurkey

Oxidative stress has been shown to play an important role in the pathogenesis of acute pancreatitis (AP). Antioxidants, alone or in combination with conventional therapy, should improve oxidative-stress-induced organ damage and therefore accelerate the rate of recovery. In recent years, substantial amounts of data about the efficiency of antioxidants against oxidative damage have been obtained from experiments with rodents. Some of these antioxidants have been found beneficial in the treatment of AP in humans; however, at present there is insufficient clinical data to support the benefits of antioxidants, alone or in combination with conven-tional therapy, in the management of AP in humans. Conflicting results obtained from experimental animals and humans may represent distinct pathophysiological mechanisms mediating tissue injury in different species. Further detailed studies should be done to clarify the exact mechanisms of tissue injury in human AP. Herein I tried to review the existing experimental and clinical studies on AP in order to determine the efficiency of antioxidants. The use of antioxidant enriched nutrition is a potential direction of clinical research in AP given the lack of clues about the efficiency and safety of antioxidant usage in patients with AP.

关键词： Acute pancreatitis Antioxidants Clinical Experimental Oxidative stress

Protective effect of low dose of melatonin against cholestatic oxidative stress after common bile duct ligation in rats

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World Journal of Gastroenterology 2005年第13期11卷 1951-1956页

作者： mukaddes Esrefoglu Mehmet Gül Memet Hanifi Emre Alaattin Polat Mukadder Ayse Selimoglu Department of Histology and Embryology Department of Physiology Department of Pediatric Gastroenterology

AIM: To investigate the role of oxidative injury and the effect of exogenous melatonin administration on liver damage induced by bile duct ligation (BDL), and second, to evaluate the role of nitric oxide (NO), a free oxygen radical, in oxidative injury. METHODS: Thirty-two Sprague-Dawley rats were assigned to four groups: sham operation (SO), BDL, BDL+melatonin, and BDL+vehicle. Cholestasis was achieved by double ligature of the common bile duct. Melatonin was injected intraperitoneally 500 μg/(kg·d) for 8 d. Hepatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides, measured as malondialdehyde (MDA), and reduced GSH. Total nitrite (NOx) concentrations were determined in hepatic homogenates. Histopathological examination was performed using a histological scoring system. RESULTS: The histopathological changes including portal inflammation, necrosis,apoptosis, focal inflammation and fibrosis were severe in the BDL and BDL+vehicle groups. There were numerous large areas of coagulation necrosis. Histological Activity Index scores of these groups were significantly higher than that of the SO group. Treatment with melatonin reduced these alterations significantly. The degree of necro-inflammation and fibrosis showed significant difference between the BDL and BDL+melatonin groups. BDL was accompanied by a significant increase in MDA and NOx, and a significant decrease in GSH levels. Mean±SE values of MDA, GSH and NOx levels of SO group were 147.47±6.69, 0.88±0.33 μmol/g and 180.70±6.58 nm/g, respectively. The values of BDL group were 200.14±21.30, 0.65±0.02 μmol/g, and 400.46±48.89 nm/g, respectively, whereas the values of BDL+melatonin group were 115.93±6.8,0.74±0.02 μmol/g, and 290.38±32.32 nm/g, respectively. Melatonin treatment was associated with a significant recovery of MDA, GSH and NOx levels. CONCLUSION: We have concluded that oxidative stress is associated with the pathogenesis of cholestatic liver damage and NO contributes to o

关键词： Cholestasis Melatonin Oxidative stress Free radicals Hepatic injury

Effectiveness of Saccharomyces boulardii in a rat model of colitis

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World Journal of Gastroenterology 2012年第44期18卷 6452-6460页

作者： Mujde Soyturk Saba mukaddes Saygili Huseyin Baskin Ozgul Sagol Osman Yilmaz Fatih Saygili Hale Akpinar Department of Gastroenterology Dokuz Eylul University School of MedicineInciraltiIzmir 35340Turkey Department of Internal Medicine Mediguven HospitalSalihli 45300ManisaTurkey Department of Clinical Microbiology Dokuz Eylul University School of MedicineInciraltiIzmir 35340Turkey Department of Pathology Dokuz Eylul University School of MedicineInciraltiIzmir 35340Turkey Department of Laboratory Animal Science Dokuz Eylul University School of MedicineInciraltiIzmir 35340Turkey Department of Gastroenterology Pamukkale University School of MedicineDenizli 20070Turkey

AIM:To investigate the effects of Saccharomyces boulardii(***) in an experimental rat model of trinitrobenzene sulfonic acid(TNBS)-induced ***:Thirty-two Wistar albino female rats were categorized into five *** the first day of the study,50 mg TNBS was administered via a rectal catheter in order to induce colitis in all rats,except those in the control *** 14 d,the rats were fed a standard diet,without the administration of any additional supplements to either the control or TNBS groups,in addition to 1 mg/kg per day *** to the *** group,1 mg/kg per day methyl prednisolone(MP) to the MP *** animals in the *** + MP group were coadministered these doses of *** and *** the study,weight loss,stool consistency,and the presence of obvious blood in the stool were evaluated,and the disease activity index(DAI) for colitis was *** intestines were examined and colitis was macro-and microscopically *** serum and tissue levels of tumor necrosis factor-α(TNF-α) and nitric oxide(NO) were determined,and fungemia was evaluated in the blood ***:The mean DAI scores for the MP and *** + MP groups was significantly lower than the TNBS group(3.69 ± 0.61 vs 4.46 ± 0.34,P = 0.018 and 3.77 ± 0.73 vs 4.46 ± 0.34,P = 0.025,respectively).While no significant differences between the TNBS and the *** or MP groups could be determined in terms of serum NO levels,the level of serum NO in the *** + MP group was significantly higher than in the TNBS and *** groups(8.12 ± 4.25 μmol/L vs 3.18 ± 1.19 μmol/L,P = 0.013;8.12 ± 4.25 μmol/L vs 3.47 ± 1.66 μmol/L,P = 0.012,respectively).The tissue NO levels in the ***,MP and *** + MP groups were significantly lower than the TNBS group(16.62 ± 2.27 μmol/L vs 29.72 ± 6.10 μmol/L,P = 0.002;14.66 ± 5.18 μmol/Lvs 29.72 ± 6.10 μmol/L,P = 0.003;11.95 ± 2.34 μmol/Lvs 29.72 ± 6.10 μmol/L,P = 0.002,respectively).The tis

关键词： Saccharomyces boulardii Rat Trinitroben-zene sulfonic acid Tumor necrOsis factor-m Nitric ox-ide Fungemia

Antioxidative effect of melatonin, ascorbic acid and N-acetylcysteine on caerulein-induced pancreatitis and associated liver injury in rats

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World Journal of Gastroenterology 2006年第2期12卷 259-264页

作者： mukaddes E■refoglu Mehmet Gül Burhan Ate■ Kadir Batoglu Mukadder Ay■e Selimoglu Department of Histology and Embryology Faculty of MedicineInonu University44280MalatyaTurkey Department of Chemistry Faculty of Science and ArtInonu University44280MalatyaTurkey Department of Biochemistry Faculty of PharmacyInonu University44280MalatyaTurkey Department of Pediatric Gastroenterology Hepatology and NitrutionFaculty of MedicineAtaturk UniversityErzurumTurkey

AIM： To investigate the role of oxidative injury in pancreatitis-induced hepatic damage and the effect of antioxidant agents such as melatonin, ascorbic acid and N-acetyl cysteine on caerulein-induced pancreatitis and associated liver injury in rats. METHODS： Thirty-eight female Wistar rats were used. Acute pancreatitis （AP） was induced by two i.p. injections of caerulein at 2-h intervals （at a total dose of 100 μg/kg ***）. The other two groups received additional melatonin （20 mg/kg ***） or an antioxidant mixture containing L（＋）-ascorbic acid （14.3 mg/***.） and N-acetyl cysteine （181 mg/kg ***.） i.p. shortly before each injection of caerulein. The rats were sacrificed by decapitation 12 h after the last injection of caerulein. Pancreatic and hepatic oxidative stress markers were evaluated by changes in the amount of lipid peroxides measured as malondialdehyde （MDA） and changes in tissue antioxidant enzyme levels, catalase （CAT） and glutathione peroxidase （GPx）. Histopathological examination was performed using scoring systems. RESULTS： The degree of hepatic cell degeneration, intracellular vacuolization, vascular congestion, sinusoidal dilatation and inflammatory infiltration showed a significant difference between caerulein and caerulein＋melatonin （P= 0.001）, and careulein and caerulein ＋ L（＋）- ascorbic acid ＋N-acetyl cysteine groups （P= 0.002）. The degree of aciner cell degeneration, pancreatic edema, intracellular vacuolization and inflammatory infiltration showed a significant difference between caerulein and caerulein ＋ melatonin （P=0.004）, and careulein and caerulein ＋ L（＋）-ascorbic acid ＋N-acetyl cysteine groups （P=0.002）. Caerulein-induced pancreatic and liver damage was accompanied with a significant increase in tissue MDA levels （P= 0.01, P= 0.003, respectively） whereas a significant decrease in CAT （P= 0.002, P=0.003, respectively） and GPx activities （P= 0.002, P= 0.03, respectively）. Melatonin and L（＋）-ascorbic acid ＋N-acetyl cysteine administratio

关键词： Caerulein Liver Melatonin Oxidative stress Pancreatitis

Role of stem cells in repair of liver injury:Experimental and clinical benefit of transferred stem cells on liver failure

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World Journal of Gastroenterology 2013年第40期19卷 6757-6773页

作者： mukaddes Esrefoglu Department of Histology and Embryology Medical FacultyBezmialem Vakif University34093 IstanbulTurkey

Although the liver has a high regenerative capacity,as a result of massive hepatocyte death,liver failure occurs. In addition to liver failure,for acute,chronic and hereditary diseases of the liver,cell transplantation therapies can stimulate regeneration or at least ensure sufficient function until liver transplantation can be performed. The lack of donor organs and the risks of rejection have prompted extensive experimental and clinical research in the field of cellular transplantation. Transplantation of cell lineages involved in liver regeneration,including mature hepatocytes,fetal hepatocytes,fetal liver progenitor cells,fetal stem cells,hepatic progenitor cells,hepatic stem cells,mesenchymal stem cells,hematopoietic stem cells,and peripheral blood and umbilical cord blood stem cells,have been found to be beneficial in the treatment of liver *** this article,the results of experimental and clinical cell transplantation trials for liver failure are reviewed,with an emphasis on regeneration.

关键词： Liver regeneration Liver failure Stem cell

PERFORMANCE STUDIES OF A FINITE ELEMENT SOFTWARE FOR LINEAR ELASTICITY ANALYSIS

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PERFORMANCE STUDIES OF A FINITE ELEMENT SOFTWARE FOR LINEAR ...

The Effect of Tramadol on Blood Glucose Levels in Rats

The 2nd Session of 2008 Workshop of Pakistan Academy of Sciences

作者： Abul Mukid Mohammad mukaddes Mohammed Mahbubul Islam Department of Industrial and Production Engineering Shahjalal University of Science and TechnologySylhet Bangladesh

ADVENTURE on Windows is a collection of finite element modules including ADVENTUREolid ported to Microsoft Windows for use in a single CPU. It supports the iterative domain decomposition method with a preconditioner for the solution of linear equations. This paper analyzed 3 dimensional linear elastic stress problems and measured the performance of the finite element software ADVENTURE on Windows. A complete finite element analysis from CAD design to visualization was performed in this research successfully. Two simple models have been considered for the linear elastic problems. Computational results show the computation time, required memory, convergence criteria and some limitations of the software.

关键词： Finite Element Method Linear Stress Analysis ADVENTURE on Windows Domain Decomposition Method Balancing Domain Decomposition

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Open Journal of Anesthesiology 2013年第1期3卷 18-22页

作者： Hacer Kara Ayse Karci mukaddes Gumustekin Murat Ormen Aydin Tasdogen Zahide Elar Department of Anesthesiology and Reanimation Dokuz Eylul University Medical SchoolizmirTurkey Department of Pharma-cology Dokuz Eylül University Medical SchoolizmirTurkey Department of Biochemistry Dokuz Eylul University Medical SchoolizmirTurkey.

The Background: Tramadol, is a central acting analgesic that possesses weak affinity for the μ-opioid receptor and modifies transmission of nociceptive impulses through inhibition of monoamine reuptake. This study was designed to determine the effect of tramadol on blood glucose levels and also to investigate whether or not alpha-2 adrenergic receptors were responsible for this effect. Methods: Twenty-five Wistar male rats were assigned to four groups to receive: Group I: saline;Group II: tramadol (1 mg·kg-1);Group III and Group IV: pretreatment with a2-receptor antagonist drugs yohimbine (1 mg·kg-1) or idazoxan (1 mg·kg-1), 30 min before administration of tramadol (1 mg·kg-1). Samples for plasma glucose measurement were withdrawn at 0, 30, 60, 90 and 120 minutes of the experiment. Results: A significant rise in blood glucose levels was observed following administration of i.v. tramadol. Pretreatment with both yohimbine and idazoxan (1 mg·kg-1) significantly attenuated tramadol-induced hyperglycemia. Conclusion: The results of the study indicate that, tramadol administered at an analgesic dose of 1 mg·kg-1 produces hyperglycemia in diethyl ether anesthetized rats. Reversal of this effect with a2-adrenoceptor blocking agents suggests that monoaminergic pathways which contribute to the analgesic action of tramadol, may have a role in the hyperglycemic action of the drug.

关键词： Hyperglycemia Tramadol a2Antagonist Antinociceptive Effect Yohimbine Idazoxan Rat

Examining the whole bowel, double balloon enteroscopy: Indications, diagnostic yield and complications

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World Journal of Gastrointestinal Endoscopy 2015年第3期7卷 247-252页

作者： Fatih Saygili Saba mukaddes Saygili Erkin Oztas Department of Gastroenterology Turkiye Yuksek Ihtisas Education and Research Hospital Department of Intensive Care Ankara University

Double balloon enteroscopy(DBE) is an advanced type of endoscopic procedure which brings the advantage of reaching the whole small bowel using anterograde or the retrograde route. This procedure is both diagnostic and interventional for a variety of small intestinal diseases, such as vascular lesions, tumors, polyps and involvement of inflammatory bowel *** indication is the diagnosis and treatment of mid-gastrointestinal bleeding according to the recent published data all over the world. The complication rates seem to be higher than conventional procedures but growing experience is lowering them and improving the procedure to be safe and well tolerated. This review is about the technique, indications, diagnostic importance and complications of DBE according to the literature growing since 2001.

关键词： Endoscopy Small bowel Double balloonenteroscopy