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Effects of Aeriscardovia aeriphila on growth performance,antioxidant functions,immune responses,and gut microbiota in broiler chickens

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第11期24卷 1014-1026页

作者： muhammad zahid farooq Xinkai WANG Xianghua YAN National Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan430070China Department of Animal Sciences University of Veterinary and Animal Sciences(Jhang Campus)Lahore54000Pakistan

Aeriscardovia aeriphila,also known as Bifidobacterium aerophilum,was first isolated from the caecal contents of pigs and the faeces of cotton-top *** species play important roles in preventing intestinal infections,decreasing cholesterol levels,and stimulating the immune *** this study,we isolated a strain of bacteria from the duodenal contents of broiler chickens,which was identified as ***,and then evaluated the effects of *** on growth performance,antioxidant functions,immune functions,and gut microbiota in commercial broiler *** were orally gavaged with ***(1×109 CFU/mL)for 21 *** results showed that *** treatment significantly increased the average daily gain and reduced the feed conversion ratio(P<0.001).The levels of serum growth hormone(GH)and insulin-like growth factor 1(IGF-1)were significantly increased following *** treatment(P<0.05).Blood urea nitrogen and aspartate aminotransferase levels were decreased,whereas glucose and creatinine levels increased as a result of *** ***,the levels of serum antioxidant enzymes,including catalase(P<0.01),superoxide dismutase(P<0.001),and glutathione peroxidase(P<0.05),and total antioxidant capacity(P<0.05)were enhanced following *** *** treatment significantly increased the levels of serum immunoglobulin A(IgA)(P<0.05),IgG(P<0.01),IgM(P<0.05),interleukin-1(IL-1)(P<0.05),IL-4(P<0.05),and IL-10(P<0.05).The broiler chickens in the *** group had higher secretory IgA(SIgA)levels in the duodenum(P<0.01),jejunum(P<0.001),and cecum(P<0.001)than those in the control *** messenger RNA(mRNA)relative expression levels of IL-10(P<0.05)and IL-4(P<0.001)in the intestinal mucosa of chickens were increased,while nuclear factor-κB(NF-κB)(P<0.001)expression was decreased in the *** group compared to the control ***-level analysis revealed Firmicutes as the main phylum,follow

关键词： Chicken Aeriscardovia aeriphila Gut health Microbiome Immune function

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Bacteroides-derived isovaleric acid enhances mucosal immunity by facilitating intestinal IgA response in broilers

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Journal of Animal Science and Biotechnology 2023年第3期14卷 1252-1273页

作者： Xinkai Wang Yifan Hu Xiaoyan Zhu Liyuan Cai muhammad zahid farooq Xianghua Yan State Key Laboratory of Agricultural Microbiology Hubei Hongshan LaboratoryFrontiers Science Center for Animal Breeding and Sustainable ProductionCollege of Animal Sciences and TechnologyHuazhong Agricultural UniversityWuhan 430070HubeiChina Department of Animal Sciences University of Veterinary and Animal Sciences(Jhang Campus)Lahore 54000Pakistan

Background:The interaction between nutrition and immunity plays a vital role in nutrient digestion,absorption,and metabolism during poultry *** studies showed that the gut microbiota contributes to the development of intestinal mucosal ***,the mechanisms by which gut microbes regulate this process remain ***:We compared the intestinal mucosal immunity and gut microbiota of Arbor Acre broilers AA(lower mucosal immunity)and Chinese native Wuliang Mountain Black-bone chickens(WLMB)(higher mucosal immunity)using 16S rDNA sequencing,transcriptomic analysis,and immunoglobulin A(IgA)antibody repertoire *** then combined 16S rDNA sequencing with transcriptomics to identify the key microbes and found that they were positively correlated with IgA ***,we transplanted candidate microbes into 1-day-old broiler to explore their role in intestinal mucosal ***,we verified the function of candidate microbial metabolites in regulating the immune function of macrophages and the intestinal-epithelial cells(IECs)using in vitro ***:WLMB performs stronger mucosal immunity than AA,including higher IgA levels,more diverse IgA antibody repertoire,and higher bacterial *** was identified as the key microbes related to the intestinal IgA *** transplantation could increase IgA concentration in the duodenal contents by enhancing the expression of IgA,polymeric immunoglobin receptor(PIgR),B cell-activating factor of the TNF family(BAFF),and activation-induced cytidine deaminase(AID)in the ***,Bacteroides-derived isovaleric acid promoted M2 macrophage polarization of macrophage via mTOR/PPAR-γ/STAT3 signaling pathways and regulated the immunologic function of IECs to produce cytokines,including interleukin(IL)-10,IL-4,BAFF,and transforming growth factor-beta(TGF-β),thus promoting IgA production in B cells by facilitating AID ***:Our study re

关键词： Bacteroides Chicken IgA Intestinal health Isovaleric acid Macrophage

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Deep Convolutional Neural Networks for Accurate Classification of Gastrointestinal Tract Syndromes

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Computers, Materials & Continua 2024年第1期78卷 1207-1225页

作者： zahid farooq Khan muhammad Ramzan Mudassar Raza muhammad Attique Khan Khalid Iqbal Taerang Kim Jae-Hyuk Cha Department of Computer Science COMSATS University IslamabadWah CampusWah47040Pakistan Department of Computer Science and Mathematics Lebanese American UniversityBeirut13-5053Lebanon Department of Computer Science HITEC UniversityTaxila47080Pakistan Department of Computer Science COMSATS University IslamabadAttock CampusAttock43600Pakistan Department of Computer Science Hanyang UniversitySeoul04763Korea

Accurate detection and classification of artifacts within the gastrointestinal(GI)tract frames remain a significant challenge in medical image *** science combined with artificial intelligence is advancing to automate the diagnosis and treatment of numerous *** to this is the development of robust algorithms for image classification and detection,crucial in designing sophisticated systems for diagnosis and *** study makes a small contribution to endoscopic image *** proposed approach involves multiple operations,including extracting deep features from endoscopy images using pre-trained neural networks such as Darknet-53 and ***,feature optimization utilizes the binary dragonfly algorithm(BDA),with the fusion of the obtained feature *** fused feature set is input into the ensemble subspace k nearest neighbors(ESKNN)*** Kvasir-V2 benchmark dataset,and the COMSATS University Islamabad(CUI)Wah private dataset,featuring three classes of endoscopic stomach images were *** assessments considered various feature selection techniques,including genetic algorithm(GA),particle swarm optimization(PSO),salp swarm algorithm(SSA),sine cosine algorithm(SCA),and grey wolf optimizer(GWO).The proposed model excels,achieving an overall classification accuracy of 98.25% on the Kvasir-V2 benchmark and 99.90% on the CUI Wah private *** approach holds promise for developing an automated computer-aided system for classifying GI tract syndromes through endoscopy images.

关键词： Feature fusion Darknet-53 Xception binary dragonfly algorithm ensemble

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Aeriscardovia aeriphila对肉鸡生长性能、抗氧化功能、免疫应答和肠道菌群的影响（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2023年第11期 1014-1027页

作者： muhammad zahid farooq 王新锴晏向华华中农业大学动物科学技术学院农业微生物资源发掘与利用全国重点实验室湖北洪山实验室动物育种与健康养殖前沿科学中心巴基斯坦兽医和动物科学大学(Jhang校区)动物科学系

Aeriscardovia aeriphila，又名Bifidobacterium aerophilum，是从猪盲肠内容物中分离出的一种双歧杆菌。***在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌，经16... 详细信息

Aeriscardovia aeriphila，又名Bifidobacterium aerophilum，是从猪盲肠内容物中分离出的一种双歧杆菌。***在预防肠道感染、降低胆固醇水平和刺激免疫系统方面具有重要作用。本研究首先从肉鸡十二指肠肠道内容物中分离出一株菌，经16S测序鉴定为***，然后我们评估了***对商用肉鸡生长性能、抗氧化功能、免疫功能和肠道菌群的影响。实验对21日龄的实验鸡进行了为期3周的灌服***(1×109 CFU/m L）处理。研究结果显示：***处理显著增加了鸡的平均日增重，降低了饲料转化率（P<0.001）。***处理后，血清生长激素（GH）和胰岛素样生长因子1(IGF-1）水平显著升高（P<0.05）。***处理降低了血尿素氮和天门冬氨酸氨基转移酶水平，增加了血糖和肌酐水平。同时，***处理显著提高了血清中抗氧化酶水平（包括过氧化氢酶（P<0.01）、超氧化物歧化酶（P<0.001）和谷胱甘肽过氧化物酶（P<0.05））及总抗氧化能力（P<0.05）。***处理显著增加了血清免疫球蛋白A(Ig A)(P<0.05）、Ig G(P<0.01）、Ig M(P<0.05）、白细胞介素-1(IL-1)(P<0.05）、IL-4(P<0.05）和IL-10(P<0.05）的水平。与对照组相比，***组肉鸡在十二指肠（P<0.01）、空肠（P<0.001）和盲肠（P<0.001）中的分泌型免疫球蛋白A(SIg A）水平显著提高。在肠道黏膜（包括十二指肠、空肠和盲肠）中，***组鸡的IL-10(P<0.05）、IL-4(P<0.001）和NF-κB(P<0.001）信使RNA(m RNA）相对表达水平高于对照组。肠道微生物门水平分析显示，两组的差异菌群主要是厚壁菌门，其次是拟杆菌门。此外，***可提高肠道中Phascolarctobacterium和Barnesiella的丰度。综上所述，灌服***可改善肉鸡的生长性能、血清抗氧化能力、免疫调节和肠道健康。因此，本研究结果可以为***在家禽生产中的应用提供理论依据。

关键词：鸡 Aeriscardovia aeriphila 肠道健康菌群免疫功能

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Exploration of Genetic Pattern of Phenological Traits in Wheat (Triticum aestivum L.) under Drought Stress

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Phyton-International Journal of Experimental Botany 2022年第12期91卷 2733-2758页

作者： Iqra Ishaaq muhammad Umer farooq Syeda Anjum Tahira Rizwana Maqbool Celaleddin Barutçular muhammad Yasir Saira Bano Zaid Ulhassan Ghassan zahid muhammad Ahsan Asghar Sajad Hussain Kocsy Gabor Ulkar Ibrahimova Jianqing Zhu Anshu Rastogi Department of Plant Breeding and Genetics Faculty of AgricultureUniversity of Agriculture FaisalabadFaisalabad38040Pakistan Department of Botany Faculty of Life SciencesUniversity of OkaraOkara56130Pakistan Department of Field Crops Faculty of AgricultureÇukurova UniversityAdana01330Turkey Department of Crop Genetics and Breeding Rice Research InstituteSichuan Agricultural UniversityChengdu Campus611130China Key Laboratory of Agriculture Products Improvement Zhejiang Agriculture and Forestry UniversityHangzhou311300China Institute of Crop Science Ministry of Agriculture and Rural Affairs Key Laboratory of Spectroscopy SensingZhejiang UniversityHangzhou310058China Department of Biotechnology Institute of Natural and Applied SciencesÇukurova UniversityAdana01330Turkey Agricultural Institute ELKH Centre for Agricultural ResearchMartonvásár2462Hungary College of Agronomy Sichuan Agricultural UniversityYa’an611130China Institute of Molecular Biology and Biotechnologies Azerbaijan National Academy of SciencesBakuAZ 1073Azerbaijan Research Institute of Crop Husbandry Ministry of Agriculture of the Azerbaijan RepublicBakuAZ 1098Azerbaijan Laboratory of Bioclimatology Department of Ecology and Environmental ProtectionPoznan University of Life SciencesPoznan60-649Poland

Drought is the major detrimental environmental factor for wheat(Triticum aestivum L.)*** exploration of genetic patterns underlying drought tolerance is of great *** we report the gene actions controlling the phenological traits using the line×tester model studying 27 crosses and 12 parents under normal irrigation and drought *** results interpreted via multiple analysis(mean performance,correlations,principal component,genetic analysis,heterotic and heterobeltiotic potential)disclosed highly significant differences among *** phenological waxiness traits(glume,boom,and sheath)were strongly *** leaf area exhibits a positive association with peduncle and spike length under *** growing degree days(heat-units)greatly influence spikelets and grains per spike,however,the grain yield/plant was significantly reduced(17.44 g to 13.25 g)under *** principal components based on eigenvalue indicated significant PCs(first-seven)accounted for 79.9%and 73.9%of total variability under normal irrigation and drought,*** investigated yield traits showed complex genetic *** genetic advance confronted a moderate to high heritability for spikelets/spike and grain yield/*** traits conditioned by dominant genetic effects in normal irrigation were inversely controlled by additive genetic effects under drought and vice *** magnitude of dominance effects for phenological and yield traits,i.e.,leaf twist,auricle hairiness,grain yield/plant,spikelets,and grains/spike suggests that selection by the pedigree method is appropriate for improving these traits under normal irrigation conditions and could serve as an indirect selection index for improving yield-oriented traits in wheat populations for drought ***,the phenotypic selection could be more than effective for traits conditioned by additive genetic effects under *** suggest five significant cross combinations base

关键词： Wheat drought phenological traits genetic variability heterotic and heterobeltiotic potential

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Entropy generation applications in flow of viscoelastic nanofluid past a lubricated disk in presence of nonlinear thermal radiation and Joule heating

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Communications in Theoretical Physics 2021年第9期73卷 37-50页

作者： Aamar Abbasi Waseh farooq M Ijaz Khan Sami Ullah Khan Yu-Ming Chu zahid Hussain M Y Malik Department of Mathematics University of Azad Jammu and Kashmir Muzaffarabad13100 Pakistan Department of Mathematics and Statistics Riphah International University I-14Islamabad 44000Pakistan Department of Mathematics COMSATS University IslamabadSahiwal 57000Pakistan Department of Mathematics Huzhou UniversityHuzhou 313000China Department of Mathematics College of SciencesKing Khalid UniversityAbha 61413Saudi Arabia

Entropy generation is the loss of energy in thermodynamical systems due to resistive forces,diffusion processes,radiation effects and chemical *** main aim of this research is to address entropy generation due to magnetic field,nonlinear thermal radiation,viscous dissipation,thermal diffusion and nonlinear chemical reaction in the transport of viscoelastic fluid in the vicinity of a stagnation point over a lubricated *** conservation laws of mass and momentum along with the first law of thermodynamics and Fick’s law are used to discuss the flow,heat and mass transfer,while the second law of thermodynamics is used to analyze the entropy and *** numbers of independent variables in the modeled set of nonlinear partial differential equations are reduced using similarity variables and the resulting system is numerically approximated using the Keller box *** effects of thermophoresis,Brownian motion and the magnetic parameter on temperature are presented for lubricated and rough *** local Nusselt and Sherwood numbers are documented for both linear and nonlinear thermal radiation and lubricated and rough *** representations of the entropy generation number and Bejan number for various parameters are also shown for lubricated and rough *** concentration of nanoparticles at the lubricated surface reduces with the magnetic parameter and Brownian *** entropy generation declines for thermophoresis diffusion and Brownian motion when lubrication effects are *** is concluded that both entropy generation and the magnitude of the Bejan number increase in the presence of *** current results present many applications in the lubrication phenomenon,heating processes,cooling of devices,thermal engineering,energy production,extrusion processes etc.

关键词： entropy generation viscoelastic fluid lubricated surface nonlinear thermal radiation Joule heating Keller box method

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Optimizing Yield and Nutrients Content in Tomato by Vermicompost Application under Greenhouse Conditions

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Natural Resources 2015年第7期6卷 457-464页

作者： Syed Ishtiaq Hyder muhammad farooq Tariq Sultan Arshad Ali muhammad Ali muhammad zahid Kiani Shahbaz Ahmad Tausif Tabssam National Agricultural Research Centre (NARC) Islamabad Pakistan

Optimizing yield and nutrients content in crop production is the high time demand of arable farming. Vermicompost furnishes one of the most promising alternatives to costly chemical fertilizer. Therefore, a greenhouse experiment was conducted at NARC, Islamabad, to investigate the effect of different levels of vermicompost [having C/N=15/1 and 14 Plant Growth Promoting Rhizobacteria (PGPR)] on growth, yield and nutrients content in hybrid tomato (National Tunnel Tomato-04-08) under greenhouse conditions during Rabi 2015. The data revealed that different rates (0.5 t·ha-1, 1 t·ha-1, 1.5 t·ha-1 and 2 t·ha-1) of vermicompost produced varied and significant (P -1) at the application of 2.0 t vermicompost ha-1 followed by 3.226 t·ha-1 where vermicompost was applied @ 1.5 t·ha-1. N, P and K content in tomato fruit and plant increased significantly with the application of increasing levels of vermicompost. The highest content of N (3.7%), P (0.67%), K (5.17%) in tomato fruit and N (3.4%), P (0.32%), K (3.2%) in tomato plant respectively were registered with soil application of vermicompost @ 2.0 t·ha-1. This study confirms that the vermicompost has a tremendous potential of plant nutrients supply for sustainable crop production.

关键词： Vermicompost PGPR Tomato Yield Nutrients and Humic Acid

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Cap-assisted endoscopy for esophageal foreign bodies:A metaanalysis

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World Journal of Meta-Analysis 2023年第1期11卷 38-46页

作者： zahid Ijaz Tarar Umer farooq Matthew L Bechtold Yezaz A Ghouri Department of Internal Medicine University of MissouriColumbiaMO 65212United States Department of Medicine Loyola UniversityChicagoIL 60153United States Department of Medicine University of Missouri-ColumbiaColumbiaMO 65212United States Department of Internal Medicine Division of Gastroenterology and HepatologyUniversity of MissouriColumbiaMO 65212United States

BACKGROUND Esophageal foreign bodies are common around the *** approaches,such as cap-assisted endoscopy,have been introduced as an alternative to conventional ***,we performed a meta-analysis ono cap-assisted endoscopy versus conventional endoscopy for removal of esophageal foreign *** To investigated the effectiveness of cap-assisted endoscopy with conventional *** An extensive literature search was performed(December 2021).For esophageal foreign body removal,cap-assisted endoscopy was compared to conventional endoscopy for procedure time,technical success of the procedure,time of foreign body retrieval,en bloc removal,and adverse event rate using odds ratio and mean *** Six studies met the inclusion criteria(n=1305).Higher odds of technical success(P=0.002)and en bloc removal(P<0.01)and lower odds of adverse events(P=0.02)and foreign body removal time(P<0.01)were observed with cap-assisted endoscopy as compared to conventional *** For esophageal foreign bodies,the technique of cap-assisted endoscopy demonstrated increased en bloc removal and technical success with decreased time and adverse events as compared to conventional techniques.

关键词： Esophageal foreign body Food bolus Endoscopy Snares Forceps Assisted devices Capassisted endoscopy

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