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Molecular detection of Leishmania species in human and animals from cutaneous leishmaniasis endemic areas of Waziristan, Khyber Pakhtunkhwa, Pakistan

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Asian Pacific Journal of Tropical Medicine 2018年第8期11卷 495-500页

作者： Mubashir Hussain shahzad Munir Abdullah Jalal Taj Ali khan Niaz muhammad Bahar Ullah Khattak Abdullah khan Irfan Ahmed Zulqarnain Baloch Nawaz Haider Bashir muhammad Ameen Jamal Kashif Rahim Humaira Mazhar Maira Riaz Noha Watany Vector Borne Diseases Lab Department of Microbiology Kohat University of Science and Technology Kohat Faculty of Plant Protection Yunnan Agricultural University Institute of Biotechnology and Genetic Engineering The University of AgriculturePeshawar Pakistan Yunnan Provincial Key Laboratory of Animal Nutrition and Feed Yunnan Agricultural University College of Veterinary Medicine South China Agricultural University Department of Animal Breeding Genetics and Reproduction Yunnan Agricultural University Beijing Key Laboratory of Genetic Engineering Drug and Biotechnology Institute of Biochemistry and Biotechnology College of Life Sciences Beijing Normal University Vector Biology Research Lab US Naval Medical Research Unit 3

Objectives: To detect Leishmania species in human patients, animal reservoirs and Phlebotomus sandflies in Waziristan, Pakistan. Methods: Tissue smears and aspirates from 448 cutaneous leishmaniasis(CL) suspected patients were analyzed. To sort out role of the reservoir hosts, skin scrapings, spleen and liver samples from 104 rodents were collected. Furthermore, buffy coat samples were obtained from 60 domestic animals. Sandflies were also trapped. All human, animals and sandfly samples were tested by microscopy, kinetoplastic PCR and internal transcribed spacer 1(ITS1) PCR followed by restriction fragment length polymorphism for detection of Leishmania species. Results: An overall prevalence of 3.83% and 5.21% through microscopy and ITS1 PCR respectively was found. However, the statistically non-significant correlation was found between area, gender, and number of lesions. The presence of rodents, sandflies, domestic animals and internally displaced people increased the risk of CL. Using ITS1-PCR-RFLP, Leishmania tropica(L. tropica) was confirmed in 106 samples while 25 of the isolates were diagnosed as Leishmania major(L. major). Similarly, 3/104 rodents were positive for L. major and 14 pools of DNA samples containing Phlebotomus sergenti sandflies were positive for L. tropica. None of samples from domestic animals were positive for leishmaniasis. Conclusions: In the present study, L. tropica and L. major are found to be the main causative agents of CL in study area. Movement of internally displaced people from CL endemic areas presents a risk for nearby CL free areas. To the best of our knowledge, we report for the first time L. major infection in rodents(Rattus rattus) and L. tropica in Phlebotomus sergenti sandflies trapped in Waziristan, Pakistan.

关键词： Leishmania major Rodents Cutaneous leishmaniasis Domestic animals ITS 1 PCR Waziristan

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Divergent consequences of bio-resources on morphological plasticity and biochemical responses in early-stage leguminous species:Sustainable productivity approaches

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Geoscience Frontiers 2024年第3期15卷 312-321页

作者： Taimoor Hassan Farooq muhammad Farrakh Nawaz muhammad Qasid Awais Shakoor Irfan Ahmad Sadaf Gul Khuram shahzad Xiaoyong Chen Bangor College China A Joint Unit of Bangor University and Central South University of Forestry and TechnologyChangsha 410004China Institute of Environmental Studies University of KarachiKarachi 75270Pakistan Department of Forestry and Range Management University of Agriculture FaisalabadPakistan Hawkesbury Institute for the Environment Western Sydney UniversityPenrithNSW 2751Australia Department of Botany University of KarachiKarachi 75270Pakistan Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesChaoyang DistrictBeijing 100101China College of Arts and Sciences Governors State UniversityUniversity ParkIL 60484USA

Inorganic resources can be detrimental to the environment when *** comparison,organic resources help balance the soil’s carbon and nitrogen(C/N)ratio,enhance soil fertility and benefit ecological *** climate crises,rapid urbanization,and fast population growth are causing many natural forests to be converted to agricultural and industrial lands to fulfill ever-increasing food and developmental *** of different bio-resources becomes necessary for sustainable productivity of available *** study explores the effects of various organic amendments on the growth,morpho-physiological and biochemical attributes of three leguminous tree species:Dalbergia sissoo,Vachellia nilotica,and Acacia ampliceps,concerning sustainable ***-year-old healthy,disease-free,and homogenous seedlings were used as study material in a greenhouse pot *** organic amendments,i.e.,compost(CMP),cow dung(CD),poultry manure(PM),and biochar(BC),along with a control(CK)treatment,were *** showed that all the organic amendments performed significantly better(P<0.05)than *** produced the most significant results,followed by BC application,while PM influence was the *** all treatments and species,the maximum values of root length,root biomass,chlorophyll content,carotenoids,catalase,and total phenolic content were recorded under the CD *** for plant height and collar diameter,no big differences were observed between CD and BC(P=0.054).While comparing species,*** growth was significantly enhanced under organic amendments,followed by *** and comparative results of studied parameters conclude that CD and BC were the most effective organic amendments,which greatly improved the growth of experimental leguminous tree species;this makes these two biofertilizers a powerful tool for sustainable agricultural *** study contributes toward an enhanced understanding of plant’

关键词： Bio-fertilizers Crop productivity Ecological sustainability Nitrogen fixation Organic cultivation Physiological responses

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Ontological Model for Cohesive Smart Health Services Management

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Computers, Materials & Continua 2023年第2期74卷 3679-3695页

作者： muhammad Raza Naqvi muhammad Waseem Iqbal Syed Khuram shahzad M.Usman Ashraf Khalid Alsubhi Hani Moaiteq Aljahdali Le Laboratoire Génie de Production de l’École Nationale d’Ingénieurs de Tarbes(LGP-INP-ENIT) Universitéde ToulouseCEDEXTarbes65016France Department of Software Engineering Superior UniversityLahore53700Pakistan Department of Informatics&Systems University of Management&TechnologyLahore54000Pakistan Department of Computer Science University of Management and TechnologySialkot51310Pakistan Department of Computer Science King Abdul-Aziz UniversityJeddah21577Saudi Arabia Faculty of Computing and Information Technology King Abdulaziz UniversityJeddah21577Saudi Arabia

Health care has become an essential social-economic concern for all stakeholders(e.g.,patients,doctors,hospitals etc.),health needs,private care and the elderly class of *** massive increase in the usage of health care Internet of things(IoT)applications has great technological evolvement in human *** are various smart health care services like remote patient monitoring,diagnostic,disease-specific remote treatments and *** applications are available in a split fashion and provide solutions for variant diseases,medical resources and remote service *** main objective of this research is to provide a management platform where all these services work as a single unit to facilitate the *** ontological model of integrated healthcare services is proposed by getting requirements from various existing healthcare *** were 26 smart health care services and 26 smart health care services to classify the knowledge-based ontological *** proposed ontological model is derived from different classes,relationships,and constraints to integrate health care *** model is developed using Protégébased on each interrelated/correlated health care service having different *** querying SPARQL protocol and RDF query language(SPARQL)were used for knowledge *** Pellet Reasoner is used to check the validity and relations coherency of the proposed ontology *** to other smart health care services integration systems,the proposed ontological model provides more cohesiveness.

关键词： Ontology internet of things smart health services integration

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Mutualistic fungal endophytes produce phytohormones and organic acids that promote japonica rice plant growth under prolonged heat stress

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第12期16卷 1011-1018页

作者： muhammad WAQAS Abdul Latif khan Raheem shahzad Ihsan ULLAH Abdur Rahim khan In-Jung LEE School of Applied Biosciences Kyungpook National University Department of Agriculture Extension UoN Chair of Oman's Medicinal Plants & Marine Natural Products University of Nizwa Institute of Biotechnology and Genetic Engineering the University of Agriculture

This study identifies the potential role in heat-stress mitigation of phytohormones and other secondary metabolites produced by the endophytic fungus Paecilomyces formosus LWL1 in japonica rice cultivar Dongjin. The japonica rice was grown in controlled chamber conditions with and without P. formosus LWL1 under no stress （NS） and prolonged heat stress （HS） conditions. Endophytic association under NS and HS conditions significantly improved plant growth attributes, such as plant height, fresh weight, dry weight, and chlorophyll content. Furthermore, P. for- mosus LWL1 protected the rice plants from HS compared with controls, indicated by the lower endogenous level of stress-signaling compounds such as abscisic acid （25.71%） and jasmonic acid （34.57%） and the increase in total protein content （18.76%-33.22%）. Such fungal endophytes may be helpful for sustainable crop production under high environmental temperatures.

关键词： Paecilomyces formosus LWL1 Plant-growth promotion Heat-stress mitigation Phytohormones Organicacids Endophytes

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Validating a point of care lactate meter in adult patients with sepsis presenting to the emergency department of a tertiary care hospital of a low-to middle-income country

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World Journal of Emergency Medicine 2017年第3期8卷 184-189页

作者： muhammad Akbar Baig Hira shahzad Erfan Hussain Asad Mian Department of Emergency Medicine Aga Khan University Hospital Department of Medicine Aga Khan University Hospital Adult Critical Care Services Aga Khan University Hospital Department of Emergency Medicine Pediatrics and Child Health Aga Khan University Hospital

BACKGROUND: Timely identifi cation of high blood lactate levels in septic patients may allow faster detection of those patients requiring immediate resuscitation. Point-of-care(POC) testing is being increasingly utilized in the emergency department(ED). We examined the accuracy and timesaving effect of a handheld POC lactate device for the measurement of fingertip and whole blood lactate as compared with reference laboratory blood testing in septic ED ***: A convenience sample of adult ED patients receiving serum lactate testing were enrolled prospectively in the ED of a multidisciplinary tertiary care hospital serving the population of one of the major cities of Pakistan. Participants underwent fi ngertip POC lactate measurement with a portable device and simultaneous whole blood sampling for analysis by both the POC lactate device and standard laboratory method. Lactate measurements were compared by intraclass correlation(ICC) and Bland and Altman ***: Forty-three septic patients were included in the study. The fingertip POC & whole blood POC lactate measurements each correlated tightly with the reference method(ICC=0.93 & ICC=0.92, respectively). Similarly at 6 hours, the fingertip POC & whole blood POC lactate measurements demonstrated satisfactory correlation with the reference method(ICC=0.95 & ICC=0.97, respectively).CONCLUSION: Fingertip POC lactate measurement is an accurate method to determine lactate levels in septic ED *** WORDS: Sepsis; Point of care; Lactate; Emergency department;

关键词： Sepsis Point of care Lactate Emergency department Pakistan

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Morpho-anatomical adaptations of dominantly grown wild Datura inoxia to wastewater resource:Productivity and ecological issues

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Geoscience Frontiers 2024年第3期15卷 248-258页

作者： Taimoor Hassan Farooq Shagufta Jabeen Awais Shakoor muhammad Saleem Arif Nadia Siddique Khuram shahzad muhammad Umair Riaz Yong Li Bangor College China a Joint Unit of Bangor University and Central South University of Forestry and TechnologyChangsha 410004HunanChina Department of Botany The Islamia University of BahawalpurBahawalpur 63100Pakistan Hawkesbury Institute for the Environment Western Sydney UniversityPenrithNSW 2751Australia Department of Environmental Sciences Government College University FaisalabadFaisalabad 38000Pakistan Department of Forestry Rangeand Wildlife ManagementThe Islamia University of BahawalpurBahawalpur 63100Pakistan Institute of Geographic Sciences and Natural Resources Research Chinese Academy of SciencesBeijing 100101China National Engineering Laboratory for Applied Technology of Forestry and Ecology in South China Central South University of Forestry and TechnologyChangsha 410004HunanChina

With the increasing global water scarcity,wastewater irrigation has become widespread,but it can have detrimental ecological *** wastewater contains valuable nutrients for plants,improper treatment or the use of untreated wastewater in irrigation can negatively impact soil fertility and plant *** study is divided into two parts:firstly,a phytosociological survey was conducted to identify plant species with the highest importance value index(IVI)in the vicinity of wastewaterirrigated ***,a comparative morpho-anatomical analysis was carried out to investigate the morpho-anatomical adaptations of the species with the highest IVI under wastewater irrigation compared to normal water *** results of the phytosociological survey revealed the presence of 51 plant species in the vicinity of the wastewater-irrigated areas,with varying relative densities and *** inoxia exhibited the highest IVI(28.79),followed by Xanthium strumarium(24.34),while Lippia nodiflora showed the lowest IVI(1.86).The morphological growth of *** was superior under normal water irrigation,but the average root length was greater under wastewater *** cell anatomy,cell thickness and cell area characteristics in the dermal,ground,and vascular tissues of the stem and root tended to be greater under wastewater ***,the opposite trend was observed in leaf anatomical analysis,possibly due to the combined effect of wastewater and climatic *** and root xylem thickness were greater under wastewater irrigation,whereas phloem thickness was higher under normal water *** number of vascular bundles in the stem,root,and leaf was higher under wastewater treatment compared to normal water irrigation,but their arrangement was circular in the latter and scattered under wastewater *** inoxia demonstrated strong adaptive potential under wastewater irrigation,as indicated by its highest relative d

关键词： Water pollution Phytosociology Structural growth Phytoremediation Agricultural irrigation Ecological concerns

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A Multi-Modal Deep Learning Approach for Emotion Recognition

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Intelligent Automation & Soft Computing 2023年第5期36卷 1561-1570页

作者： H.M.shahzad Sohail Masood Bhatti Arfan Jaffar muhammad Rashid The Superior University LahorePakistan National University of Technology IslamabadPakistan Intelligent Data Visual Computing Research(IDVCR) LahorePakistan

In recent years,research on facial expression recognition(FER)under mask is *** a mask for protection from Covid 19 has become a compulsion and it hides the facial expressions that is why FER under the mask is a difﬁcult *** prevailing unimodal techniques for facial recognition are not up to the mark in terms of good results for the masked face,however,a multi-modal technique can be employed to generate better *** proposed a multi-modal methodology based on deep learning for facial recognition under a masked face using facial and vocal *** multimodal has been trained on a facial and vocal *** have used two standard datasets,M-LFW for the masked dataset and CREMA-D and TESS dataset for vocal *** vocal expressions are in the form of audio while the faces data is in image form that is why the data is *** order to make the data homogeneous,the voice data is converted into images by taking spectrogram.A spectrogram embeds important features of the voice and it converts the audio format into the ***,the dataset is passed to the multimodal for *** network and the experimental results demonstrate that the proposed multimodal algorithm outsets unimodal methods and other state-of-the-art deep neural network models.

关键词： Deep learning facial expression recognition multi-model neural network speech emotion recognition spectrogram covid-19

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Automatic Eyewitness Identification During Disasters by Forming a Feature-Word Dictionary

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Computers, Materials & Continua 2022年第9期72卷 4755-4769页

作者： shahzad Nazir muhammad Asif Shahbaz Ahmad Hanan Aljuaid Shahbaz Ahmad Yazeed Ghadi Zubair nawaz Department of Computer Science National Textile UniversityFaisalabadPakistan Computer Sciences Department College of Computer and Information SciencesPrincess Nourah bint Abdulrahman University(PNU)P.O.Box 84428Riyadh 11671Saudi Arabia Department of Software Engineering and Computer Science Al Ain UniversityAbu DhabiUnited Arab Emirates Department of Data Science University of the PunjabPakistan

Social media provide digitally interactional technologies to facilitate information sharing and exchanging ***,in case of disasters,a massive corpus is placed on platforms such as *** accounts can benefit humanitarian organizations and agencies,but identifying the eyewitness Tweets related to the disaster from millions of Tweets is *** approaches have been developed to address this kind of *** recent state-of-the-art system was based on a manually created dictionary and this approach was further refined by introducing linguistic ***,these approaches suffer from limitations as they are dataset-dependent and not *** this paper,we proposed a method to identify eyewitnesses from *** experiment,we utilized 13 features discovered by the pioneer of this domain and can classify the tweets to determine the *** each feature,a dictionary of words was created with the Word Dictionary Maker algorithm,which is the crucial contribution of this *** algorithm inputs some terms relevant to a specific feature for its initialization and then creates the words ***,keyword matching for each feature in tweets is *** a feature exists in a tweet,it is termed as 1;otherwise,***,for 13 features,we created a file that reflects features in each *** classify the tweets based on features,Naïve Bayes,Random Forest,and Neural Network were *** approach was implemented on different disasters like earthquakes,floods,hurricanes,and Forest *** results were compared with the state-of-the-art linguistic rule-based system with 0.81 F-measure *** the same time,the proposed approach gained a 0.88 value of *** results were comparable as the proposed approach is not ***,it can be used for the identification of eyewitness accounts.

关键词： Word dictionary social media eyewitness identification disasters

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Influence of Plant Growth Promoting Rhizobacterial Inoculation on Wheat Productivity Under Soil Salinity Stress

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Phyton-International Journal of Experimental Botany 2019年第2期88卷 119-129页

作者： muhammad Zafar-ul-Hye Tariq shahzad Bhutta muhammad Shaaban Shahid Hussain muhammad Farooq Qayyum Umar Aslam Zahir Ahmad Zahir Department of Soil Science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultanPakistan Institute of Soil and Environmental Sciences Faculty of AgricultureUniversity of AgricultureFaisalabadPakistan.

Soil salinity affects the growth and yield of *** stress of soil salinity on plants can be mitigated by inoculation of plant growth promoting bacteria(PGPR).The influence of PGPR inoculation on wheat(Triticum aestivum L.)crop productivity under salinity stress has not been properly addressed so ***,the present study was conducted to investigate the effects of various PGPR strains(W14,W10 and 6K;alone and combined)at several growth attributes of wheat plant under different soil salinity gradients(3,6 and 9 dS m-1).The growth attributes of wheat(height,roots,shoots,spikes,grains quality,biological and economical yield,nutrients nitrogen,phosphorus and potassium in grains)were highly affected by salinity and decreased with increasing salinity *** PGPR inoculation substantially promoted growth attributes of wheat and prominent results were observed in W14×W10×6K treatment at all salinity *** results suggest that inoculation of PGPR is a potential strategy to mitigate salinity stress for improving wheat growth and yield.

关键词： PGPR abiotic stress soil salinity ethylene wheat

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Ion-acoustic wave structures in the fluid ions modeled by higher dimensional generalized Korteweg-de Vries-Zakharov-Kuznetsov equation

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Journal of Ocean Engineering and Science 2023年第6期8卷 623-635页

作者： U.Younas J.Ren muhammad Z.Baber muhammad W.Yasin T.shahzad State Key Laboratory of Power Grid Environmental Protection/Henan Academy of Big Data Zhengzhou UniversityZhengzhou 450001China Department of Mathematics and Statistics The University of LahoreLahorePakistan Department of mathematics University of NarowalNarowalPakistn Department of Basic Sciences and Humanities Narowal CampusUniversity of Engineering and Technology Lahore 54890Pakistan

In this paper,the higher dimensional generalized Korteweg-de-Varies-Zakharov-Kuznetsov(gKdV-ZK)equation is under *** model is used in the field of plasma physics which describes the effects of magnetic field on the weak ion-acoustic *** have applied two techniques,called asφ^(6)-model expansion method and the Hirota bilinear method(HBM)to explore the diversity of wave *** solutions are expressed in the form of hyperbolic,periodic and Jacobi elliptic function(JEF)***,the solitary wave solutions are also extracted.A comparison of our results to well-known results is made,and the study concludes that the solutions achieved here are ***,3-dimensional and contour profiles of achieved outcomes are drawn in order to study their dynamics as a function of parameter *** the basis of the obtained results,we can assert that the pro-posed computational methods are straightforward,dynamic,and well-organized,and will be useful for solving more complicated nonlinear problems in a variety of fields,particularly in nonlinear sciences,in conjunction with symbolic ***,our discoveries provide an important milestone in comprehending the structure and physical behavior of complex *** hope that our findings will contribute significantly to our understanding of ocean *** study,we hope,is appropriate and will be of significance to a broad range of experts involved in ocean engineering models.

关键词： Wave solutions gKdV-ZK equation Bilinear transformation φ^(6)-model expansion method

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