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Dramatic changes in anatomical traits of a C4 grass Chrysopogon serrulatus Trin.(Poaceae)over a 1000 m elevational gradient

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Journal of Mountain Science 2023年第5期20卷 1316-1335页

作者： FATIMA Sana HAMEED Mansoor AHMAD Farooq AHMAD muhammad sajid Aqeel ANWAR Majid MUNIR Mahwish ASHRAF muhammad SHAH Syed Mohsan Raza BASHARAT Sana AHMAD Iftikhar KHALIL Sangam Department of Botany Government Sadiq College Women UniversityBahawalpur 53100Pakistan Department of Botany University of Agriculture FaisalabadFaisalabad 38040Pakistan Department of Pharmacy Riphah International University Lahore 53770Pakistan Institute of Molecular Biology and Genetic Engineering The University of LahoreLahore 53770Pakistan Department of Botany Division of Science and TechnologyUniversity of EducationLahore 53770Pakistan Department of Forestry Range&Wildlife ManagementThe Islamia University of BahawalpurBahawalpur 53100Pakistan

Chrysopogon serrulatus(false beard-grass)is a dominant component of vegetation in the foothills of the *** study whole plant morphology,individuals of *** were collected from three plots at each of six locations spanning from 400 to 1,400 *** population colonizing the highest elevation modifications in different plant *** showed increased metaxylem number and *** the stem,especially outside of the vascular tissue,there was intensive sclerification indicative of increased xeromorphy as a survival *** the highest elevation,leaves were wider;aerenchyma formation and increased sclerification were noted in the leaf sheath;and a greater proportion of storage parenchyma was observed in the leaf blade,all indicators of *** contrast,leaves at lower elevations had xeric morphological features such as increased epidermal thickness,sclerification and more developed metaxylem *** conclusion,shifting of morphological features in below-and above-ground plant parts of *** were linked to shifts in environmental factors along this elevation gradient,thus enabling the successful distribution of this species along this elevation gradient.

关键词： Adaptive component Storage parenchyma Sclerification Metaxylem Aerenchyma

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X-Band Power Amplifier for Next Generation Networks Based on MESFET

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China Communications 2017年第4期14卷 11-19页

作者： muhammad Saad Khan Hongxin Zhang Fan Zhang Sulman Shahzad Rahat Ullah sajid Ali Qasim Ali Arain Manzoor Ahmed School of Electronic Engineering Beijing University of Posts and Telecommunications (BUPT) Beijing 100876 China Beijing Key Laboratory of Work Safety Intelligent Monitoring Beijing University of Posts and Telecommunications Beijing 100876 china College of Information Science and Electrical Engineering Zhejiang University Hangzhou Zhejiang 310027china Electrical Engineering Department University College of Engineering & Technology Bahauddin Zakariya University Multan Pakistan State Key Laboratory of Information Photonics and Optical Communications Beijing University of Posts and Telecommunications Beijing 100876 China Department of Coxnputer Science D.G. Khan Campus. University of Education Lahore Pakistan Future lnternet & Communication Lab (FIB) Department of Electronic Engineering Tsinghua University Beijing 100084 China

Advanced wireless standards of communication like 3GPP and LTE are becoming more and more efficient and with this evolution of communication systems mobile equipment is also become smaller and smaller. Power amplifier designing has become a very crucial task in this era where efficiency and size are the main concern of any designer. In this paper we have design and analyzed X-band Class E Metal-semiconductor field effect transistor(MESFET) based Power Amplifier. This device targets the devices which use OFDM technique to improve their spectral efficiency for the next generation communication systems. Microstrip lines are used to achieve small size for our design instead of lumped components. Load Pull measurements are used to get MESFET input and output impedances optimum values. For linear and non linear operation small signal mathematical model of the design is used. To reduce thermal losses FR4 substrate is used to increase PA efficiency. Our designs shows small values of input and output return loss of about-22.3d B and-23.716 d B achieving a high gain of about25.6 d B respectively, with PAE of about 30 % having stability factor greater than 1 and 21.894 d Bm of output power.

关键词： MESFET X-band power amplifier advance design system PAE LTE

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Role of SNPs in determining QTLs for major traits in cotton

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Journal of Cotton Research 2019年第1期2卷 64-76页

作者： MAJEED sajid RANA Iqrar Ahmad ATIF Rana muhammad ALI Zulfiqar HINZE Lori AZHAR muhammad Tehseen Department of Plant Breeding and Genetics University of Agriculture Faisalabad Pakistan Centre of Advanced Studies in Agriculture and Food Security/Center of Agricultural Biochemistry and Biotechnology University of Agriculture Faisalabad Pakistan Institute of Plant Breeding and Biotechnology Muhammad Nawaz Sharif University of AgricultureMultan Pakistan US Department of Agriculture Agricultural Research ServiceCollege StationTXUSA

A single nucleotide polymorphism is the simplest form of genetic variation among individuals and can induce minor changes in phenotypic,physiological and biochemical *** polymorphism induces various mutations that alter the sequence of a gene which can lead to observed changes in amino *** assays have been developed for identification and validation of these *** method has its own advantages and disadvantages but genotyping by sequencing is the most common and most widely used *** markers are also associated with several desirable traits like yield,fibre quality,boll size and genes respond to biotic and abiotic stresses in *** in yield related traits are of interest to plant *** quantitative trait loci with novel functions have been identified in cotton by using these *** information can be used for crop improvement through molecular breeding *** this review,we discuss the identification of these markers and their effects on gene function of economically important traits in cotton.

关键词： Abiotic stresses Biotic stresses Cotton Earliness Genotyping by sequencing

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Yellow Vein Mosaic Disease in Okra(Abelmoschus esculentus L.):An Overview on Causal Agent,Vector and Management

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Phyton-International Journal of Experimental Botany 2021年第6期90卷 1573-1587页

作者： Mustansar Mubeen Yasir Iftikhar Aqleem Abbas Mazhar Abbas muhammad Zafar-ul-Hye Ashara sajid Faheema Bakhtawar Department of Plant Pathology College of AgricultureUniversity of SargodhaSargodha40100Pakistan State Key Laboratory of Agricultural Microbiology and Provincial Key Laboratory of Plant Pathology of Hubei Province College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhan430070China Department of Agriculture and Agribusiness Management University of KarachiKarachi75270Pakistan Department of Soil Science Faculty of Agricultural Sciences and TechnologyBahauddin Zakariya UniversityMultan60800Pakistan

Okra(Abelmoschus esculentus L.)belongs to the Malvaceae family and is one of the most essential and popular vegetables *** is rich in proteins,carbohydrates,and *** and biotic factors threaten okra *** yellow vein mosaic disease(OYVMD)is the most destructive disease of *** causal agent,[i.e.,Okra yellow vein mosaic virus(OYVMV)]of this disease belongs to the family Geminiviridae and genus *** is a monopartite with additional ssDNA *** virus has two components DNA-A for protein coding and DNA-B for symptoms *** transmits OYVMV in persistent *** symptoms of OYVMV infected okra plants are chlorosis,dwarfing,and yellowing of veins and *** temperatures with moderate rainfall enhance the development of OYVMV disease and the whitefly ***,high humidity with low temperature and rainfall has no significant role in developing the OYVMD and whitefly ***,the virus also affects the secondary metabolites in the infected okra *** virus can be managed through various strategies including the application of plant defense activators,the development of resistant varieties and by controlling its vector via pesticides and plant *** plant defense activators such as monopotassium phosphate(KH2PO),salicylic acid,benzoic acid,and citric acid enhance resistance in okra against *** addition,the resistance to OYMVD can also be achieved by successfully incorporating high yielding but resistant cultivars of acceptable *** this review,we have discussed history,economic impact,symptomology,disease development under a natural environment,genetics and management of OYVMV.

关键词： Okra OYVMV symptoms vectors epidemiological factors management

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Induced Production of Exoglucanase, and β-Glucosidase from Fungal Co-Culture of T. virideand G. lucidum

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Advances in Bioscience and Biotechnology 2014年第5期5卷 426-433页

作者： Tanzila Shahzadi Zahid Anwar Zafar Iqbal Awais Anjum Tahir Aqil Bakhtawar Arroha Afzal muhammad Kamran sajid mehmood muhammad Irshad Agriculture Biotechnology National Institute for Biotechnology and Genetic Engineering Faisalabad Pakistan Department of Biochemistry Nawaz Sharif Medical College University of Gujrat Gujrat Pakistan Department of Botany University of Gujrat Gujrat Pakistan Department of Microbiology Quaid-e-Azam University Islamabad Pakistan

In the present study, a co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase using cheap and easily available agro-industrial residue corn stover as growth supporting substrate. Various physio-chemical and nutritional variables were optimized using classical and completely randomized designs for induced production of exoglucanase, and β-glucosidase from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state fermentation (SSF). Analysis profile showed that when the conditions of the SSF medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with 6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ± 6.5 U/mL) and β-glucosidase (255 ± 3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.

关键词： Process Optimization SSF Co-Culture T. viride G. lucidum

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Seroprevalence of HDV among non-hospitalized HBs Ag positive patients from KPK-region of Pakistan

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Asian Pacific Journal of Tropical Biomedicine 2016年第7期6卷 609-613页

作者： Ismail Jalil muhammad Arshad Zara Rafaque Fazle Raziq Robina Wazir sajid Malik Javid Iqbal Dasti Department of Microbiology Qauid-i-Azam University Islamabad Department of Pathology Hayatabad Medical Complex Peshawar Department of Pathology Khyber Teaching Hospital Peshawar Department of Animal Sciences Qauid-i-Azam University Islamabad

Objective: To study the seroprevalence of hepatitis B virus(HBV) and hepatitis delta virus(HDV) infections in patients visiting outpatient department of a major tertiary care hospital in Khyber Pakhtunkhwa region of ***: Blood samples were collected from non-hospitalized patients. Serological analysis was done by ELISA and viral DNA was amplified by PCR. The amplified DNA was analyzed by agarose gel ***: Altogether, 946 blood samples were screened, overall percentage of HBs Agpositive patients remained 22.41%(prevalence: 224.10/1 000; CI: 0.197 5 ± 0.250 7) with the highest incidence rates among relatively younger age groups(20–29 years). The prevalence of HBV–HDV co-infection was found to be 46.75/1 000; CI: 0.031 8 ± 0.061 *** HBs Ag-positive patients, anti-HBc-total was detected in 86.79% while 25.00% were positive for anti-HBc-immunoglobulin M. Similarly, among these patients, HBV DNA was detected in 64.13% and 10.85% were co-infected with HDV. Different symptoms were associated with the prevailing infection, including malaise(62%), anorexia(66%) and fatigue(73%). The most commonly associated symptom was abdominal discomfort. Among these patients, certain risk factors, including surgery, visit to dentist and intravenus infusions were frequently associated with the infection(x^2= 95.23; df = 11; P < 0.000 1).Conclusions: Overall, this study confirmed higher prevalence of active HBV/HDV infection, among young patients from Khyber Pakhtunkhwa region having no prior history of viral hepatitis.

关键词： HBV HCV HDV Risk factors Co-infection

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Advances in lignocellulosic biotechnology: A brief review on lignocellulosic biomass and cellulases

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Advances in Bioscience and Biotechnology 2014年第3期5卷 246-251页

作者： Tanzila Shahzadi sajid mehmood muhammad Irshad Zahid Anwar Amber Afroz Nadia Zeeshan Umer Rashid Kalsoom Sughra Department of Biochemistry and Molecular Biology University of Gujrat Gujrat Pakistan Department of Biochemistry Nawaz Sharif Medical College University of Gujrat Gujrat Pakistan

From the last few decades, there has been an increasing research interest in the value of lignocellulosic biomass. Lignoellulosic biomass is an inexpensive, renewable abundant and provides a unique natural resource for large-scale and cost-effective bio-energy collection. In addition, using lignocellulosic materials and other low-cost biomass can significantly reduce the cost of materials used for ethanol production. Therefore, in this background, the rapidly evolving tools of biotechnology can lower the conversion costs and also enhance a yield of target products. In this context, a biological processing presents a promising approach to converting lignocellulosic materials into energy-fuels. The present summarized review work begins with an overview on the physio-chemical features and composition of major agricultural biomass. The information is also given on the processing of agricultural biomass to produce industrially important enzymes, e.g., ligninases or cellulases. Cellulases provide a key opportunity for achieving tremendous benefits of biomass utilization.

关键词： Lignicellulosic Biomass Eco-Friendly Bio-Ethanol Industrial Enzyme

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Authentication of Vehicles and Road Side Units in Intelligent Transportation System

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Computers, Materials & Continua 2020年第7期64卷 359-371页

作者： muhammad Waqas Shanshan Tu Sadaqat Ur Rehman Zahid Halim sajid Anwar Ghulam Abbas Ziaul Haq Abbas Obaid Ur Rehman Beijing Key Laboratory of Trusted Computing Faculty of Information TechnologyBeijing University of TechnologyBeijing100124China Faculty of Computer Science&Engineering Ghulam Ishaq Khan Institute of Engineering Sciences&TechnologyTopi23460Pakistan Beijing Electro-Meahnical Engineering Institute Beijing100074China Faculty of Electrical Engineering Ghulam Ishaq Khan Institute of Engineering Sciences&TechnologyTopi23460Pakistan Department of Electrical Engineering Sarhad University of Science and Information TechnologyPeshawar25000Pakistan

Security threats to smart and autonomous vehicles cause potential consequences such as traffic accidents,economically damaging traffic jams,hijacking,motivating to wrong routes,and financial losses for businesses and *** and autonomous vehicles are connected wirelessly,which are more attracted for attackers due to the open nature of wireless *** of the problems is the rogue attack,in which the attacker pretends to be a legitimate user or access point by utilizing fake *** figure out the problem of a rogue attack,we propose a reinforcement learning algorithm to identify rogue nodes by exploiting the channel state information of the communication *** consider the communication link between vehicle-to-vehicle,and *** evaluate the performance of our proposed technique by measuring the rogue attack probability,false alarm rate(FAR),mis-detection rate(MDR),and utility function of a receiver based on the test threshold values of reinforcement learning *** results show that the FAR and MDR are decreased significantly by selecting an appropriate threshold value in order to improve the receiver’s utility.

关键词： Intelligent transportation system authentication rogue attack

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Small bowel malignant melanoma presenting as a perforated jejunal diverticulum:a case report and literature review

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Gastroenterology Report 2016年第1期4卷 80-83,I0003页

作者： Richard C.Newton Nicholas Penney Nicholas Nind muhammad S.sajid Parvinder Sains The Department of General Surgery Worthing HospitalWestern Sussex Hospitals NHS Foundation TrustWorthingWest SussexN112DHUK The Department of Histopathology Worthing HospitalWestern Sussex Hospitals NHS Foundation TrustWorthingWest SussexN112DHUK

Although usually harmless and asymptomatic,jejuno-ileal diverticulae are associated with various non-specific gastrointestinal symptoms,and rarely cause surgical *** case report describes the presentation and management of a patient with an acute abdomen,whose jejunal diverticulum was ***,histopathological assessment demonstrated malignant melanoma lining the *** this was primary or metastatic is discussed,together with a synopsis of the literature on small bowel diverticulae.

关键词： malignant melanoma intestinal perforation jejunal diverticulum

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A human monoclonal antibody neutralizes SARS-CoV-2 Omicron variants bytargeting the upstream region of spike protein HR2 motif

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hLife 2024年第3期2卷 126-140页

作者： Hang Su Jun Zhang Zhenfei Yi sajid Khan Mian Peng Liang Ye Alan Bao Han Zhang Guangli Suo Qian Li Housheng Zheng Dandan Wu Thomas J.Kipps Lanfeng Wang Zhenghong Lin Suping Zhang Shenzhen Key Laboratory of Precision Medicine for Hematological Malignancies Guangdong Key Laboratory for Genome Stability andHuman Disease PreventionDepartment of PharmacologySchool of Basic Medical SciencesBase for International Science and TechnologyCooperation:Carson Cancer Stem Cell Vaccines R&D CenterInternational Cancer CenterShenzhen University Medical SchoolShenzhenUniversityGuangdongChina School of Life Sciences Chongqing UniversityChongqingChina The Center for Microbes Development and HealthCAS Key Laboratory of Molecular Virology&ImmunologyShanghai Institute of Immunityand InfectionChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghaiChina College of Life Sciences University of Chinese Academy of SciencesBeijingChina Department of Critical Care Medicine Shenzhen Luohu People’s HospitalGuangdongChina University of California BerkeleyCaliforniaUSA Xenta Biomedical Science Co. LtdGuangdongChina CAS Key Laboratory of Nano-Bio Interface Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesJiangsuChina Center of Novel Therapeutics University of CaliforniaSan DiegoCaliforniaUSA

The continuous emergence of new severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants meansthere is a need to explore additional strategies to develop broad-spectrum vaccines or therapeutics for individuals remaining at risk of coronavirus disease 2019(COVID-19).Neutralizing monoclonal antibody(mAb)that binds to theconserved S2 subunit of the SARS-CoV-2 spike(S)protein alone,or in combination with mAb that binds to the receptor-binding domain(RBD)of S protein,might be effective in eliciting protection from infection by a variety of SARS-CoV2 *** high-throughput single-cell immunoglobulin sequencing of B cells from COVID-19-convalescent donors,we identified a high-affinity S2-specific mAb-39,that could inhibit original SARS-CoV-2 strain,Omicron BA.1,BA.2.86,BA.4,BA.5,and EG.5.1 S protein-mediated membrane fusion,leading to the neutralization of these ***,mAb-39 could also improve the neutralizing activity of anti-RBD antibody against the highlyneutralization-resistant Omicron *** docking and point mutation analyses revealed that mAb-39 recognized epitopes within the conserved upstream region of the heptad repeat 2(HR2)motif of the S2 ***,these findings demonstrate that targeting the conserved upstream region of the HR2 motif(e.g.,using mAbs)provides anovel strategy for preventing the infection of SARS-CoV-2 and its variants.

关键词： coronavirus disease 2019(COVID-19) severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variants monoclonal antibody upstream region of heptad repeat 2(HR2) immunoglobulin repertoiresequencing

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