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The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors

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Journal of Pharmaceutical Analysis 2021年第2期11卷 174-182页

作者： Mirza muhammad Faran Ashraf Baig Chengfei Zhang muhammad furqan akhtar Ammara Saleem Jahanzeb Mudassir Laboratory of Biomedical&Pharmaceutical Engineering of Stem Cells Research Restorative Dental SciencesFaculty of DentistryThe University of Hong Kong999077Hong KongPR China State Key Laboratory of Analytical Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023PR China Faculty of Pharmacy Bahauddin Zakariya UniversityMultan60000Pakistan Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan

DNA-nanotechnology-based nano-architecture scaffolds based on circular strands were designed in the form of DNA-nanowires(DNA-NWs) as a polymer of DNA-triangles. Circularizing a scaffold strand(84-NT) was the critical step followed by annealing with various staple strands to make stiff DNAtriangles. Atomic force microcopy(AFM), native polyacrylamide gel electrophoresis(PAGE), UVanalysis, MTT-assay, flow cytometry, and confocal imaging were performed to assess the formulated DNA-NWs and cisplatin(CPT) loading. The AFM and confocal microscopy images revealed a uniform shape and size distribution of the DNA-NWs, with lengths ranging from 2 to 4 mm and diameters ranging from 150 to 300 nm. One sharp band at the top of the lane(500 bp level) with the loss of electrophoretic mobility during the PAGE(native) gel analysis revealed the successful fabrication of DNA-NWs. The loading efficiency of CPT ranged from 66.85% to 97.35%. MTT and flow cytometry results showed biocompatibility of the blank DNA-NWs even at 95% concentration compared with the CPT-loaded DNANWs. The CPT-loaded DNA-NWs exhibited enhanced apoptosis(22%) compared to the apoptosis(7%)induced by the blank DNA-NWs. The release of CPT from the DNA-NWs was sustained at < 75% for 6 h in the presence of serum, demonstrating suitability for systemic applications. The IC_(50) of CPT@DNA-NWs was reduced to 12.8 nM CPT, as compared with the free CPT solution exhibiting an IC_(50) of 51.2 n *** imaging revealed the targetability, surface binding, and slow internalization of the DNA-NWs in the scavenger-receptor-rich cancer cell line(HepG2) compared with the control cell line.

关键词： Cisplatin(CPT) DNA-nanowires(DNA-NWs) HepG2 resistant cancer cells Scavenger receptors

Corrigendum to“The effective transfection of a low dose of negatively charged drug-loaded DNA-nanocarriers into cancer cells via scavenger receptors”[Journal of Pharmaceutical Analysis volume 11(2021)174e182]

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Journal of Pharmaceutical Analysis 2022年第4期12卷 698-700页

作者： Mirza muhammad Faran Ashraf Baig Chengfei Zhang muhammad furqan akhtar Ammara Saleem Jahanzeb Mudassir Laboratory of Biomedical&Pharmaceutical Engineering of Stem Cells Research Restorative Dental SciencesFaculty of DentistryThe University of Hong KongHong Kong999077PR China State Key Laboratory of Analytical Chemistry for Life Sciences School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023PR China Faculty of Pharmacy Bahauddin Zakariya UniversityMultan 60000Pakistan Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan

The authors regret to inform that Figs.4,6,and 7 in the original article were wrongly *** corrected figures appear below:The authors would like to apologize for any inconvenience caused.

关键词： Cor loaded cancer

Chemical characterization, docking studies, anti-arthritic activity and acute oral toxicity of Convolvulus arvensis L. leaves

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Asian Pacific Journal of Tropical Biomedicine 2020年第10期10卷 442-451页

作者： Uzma Saleem Shingraf Zaib Sana Khalid Fareeha Anwar muhammad furqan akhtar Bashir Ahmad Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadPakistan Riphah Institute of Pharmaceutical Sciences Riphah International University LahorePakistan

Objective: To evaluate acute oral toxicity and anti-arthritic activity of the methanolic extract of Convolvulus arvensis L. ***: Safety was assessed by acute oral toxicity(OECD 425) study. Anti-arthritic activity was explored by in vitro(inhibition of protein denaturation) and in vivo(Complete Freund’s adjuvantinduced arthritis and carrageenan-induced inflammation) methods. Antioxidant potential was determined by assessing ferric reducing power, DPPH inhibition, and H2O2 scavenging assays. Furthermore, molecular docking was done to check interactions between the plant constituents and cyclooxygenases(COX-1 and COX-2). Quercetin, gallic acid, caffeic acid, syringic acid, sinapic acid, and vanillic acid were quantified by HPLC and eight compounds were identified by GC-MS analysis. Results: No mortality and abnormality in biochemical parameters were observed in the toxicity study. Histological analysis of vital organs also supported these biochemical results. The in vitro and in vivo studies showed that the methanolic extract of leaves of Convolvulus arvensis exhibited dose-dependent anti-arthritic and anti-oxidant potential. Molecular docking showed better interactions of plant compounds with cyclooxygenases as compared to standard ibuprofen. Conclusions: Convolvulus arvensis exhibits strong anti-arthritic activity, justifying the traditional use of the herbal drug.

关键词： Antioxidant organs herbal

Argemone mexicana extract alleviates gastrointestinal disorders by stimulating muscarinic receptors and blocking voltage-gated L-type calcium channels

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Asian Pacific Journal of Tropical Biomedicine 2021年第5期11卷 214-221页

作者： Rabia Iqbal Irfan Hamid Khalid Hussain Janbaz muhammad furqan akhtar Ammara Saleem Ali Sharif Sohaib Peerzada Bushra akhtar Kashif Sohail Sajid Ali Faculty of Pharmacy The University of LahoreLahorePakistan Cadson College of Pharmacy University of the PunjabLahorePakistan Department of Pharmacology Bahauddin Zakariya UniversityMultanPakistan Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahore CampusLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan Department of Pharmacology Faculty of Pharmaceutical and Allied health SciencesLahore College for Women UniversityLahorePakistan Institute of Pharmacy Physiology and PharmacologyUniversity of AgricultureFaisalabadPakistan Akson College of Pharmacy Mirpur University of Science and TechnologyMirpurAJKPakistan

Objective:To investigate the pharmacological potential of Argemone mexicana in treating constipation and emesis by using in vitro and in vivo ***:The spasmogenic and spasmolytic effects were evaluated on isolated rabbit jejunum fragments loaded in a tissue organ *** response was recorded with an isotonic transducer attached with Power Lab Data Acquisition *** laxative and antiemetic activities were assessed in BALB-c mice and poultry chicks challenged with carbamylcholine and copper sulphate stimulated emesis,***:The total phenolic and total flavonoids contents of the extract were(267.75±5.77)mg GAE/g and(73.86±6.01)mg QE/g,*** mexicana extract exerted spasmogenic effect on isolated rabbit jejunum segments with an EC_(50)value of 0.016 mg/m L,which was blocked by atropine(0.3μM).Argemone mexicana extract exerted spasmolytic effect in atropine treated jejunum fragments with an EC_(50)value of 2.185 mg/***,Argemone mexicana extract relaxed potassium(80 mM)-induced contractions(EC_(50):9.07 mg/mL),similar to a standard drug *** calcium channel blocker activity was confirmed by a rightward shift of concentration-response curve of calcium in the presence of Argemone mexicana extract(1-5 mg/mL)and verapamil(0.1-1μM).In addition,the extract increased the distance travelled by a charcoal in the gastrointestinal tract and exhibited antiemetic effect on copper sulphate induced emesis in ***:Argemone mexicana shows cholinergic agonist and calcium channel blocker activities,as well as antiemetic *** may be used as a potential agent for treating gastrointestinal disorders.

关键词： Argemone mexicana Muscarinic agonist Calcium channel blocker Constipation Emesis

Tylophora hirsuta L. leaf extract attenuates alloxan-induced diabetes in mice by suppressing oxidative stress and α-amylase

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Asian Pacific Journal of Tropical Biomedicine 2021年第9期11卷 394-404页

作者： muhammad furqan akhtar Arsalan Shagufta Ammara Saleem Mirza muhammad Faran Ashraf Baig Ali Sharif Azhar Rasul Mohamed M.Abdel-Daim Riphah Institute of Pharmaceutical Sciences Riphah International UniversityLahore CampusLahorePakistan Department of Pharmacology Faculty of Pharmaceutical SciencesGovernment College University FaisalabadFaisalabadPakistan Laboratory of Stem Cells Research and Biomedical Engineering for Novel Biofunctional and Pharmaceutical NanomaterialsFaculty of DentistryPrince Philip Dental HospitalThe University of Hong KongHong Kong 999077China Institute of Pharmacy Faculty of Pharmaceutical and Allied Health SciencesLahore College for Women UniversityJail RoadLahorePakistan Department of Zoology Government College University FaisalabadFaisalabadPakistan Pharmacology Department Faculty of Veterinary MedicineSuez Canal UniversityIsmailia 41522Egypt

Objective:To evaluate the antidiabetic potential of leaf extracts of Tylophora hirsuta(***).Methods:The methanolic and ethyl acetate extracts of *** leaves were analyzed by high pressure liquid *** vitro antioxidant activity was determined by ferric ion reduction,1,1-diphenyl-2-picrylhydrazyl,and hydrogen peroxide scavenging *** vitro alpha amylase(α-amylase)inhibitory activity of the plant extracts was *** vivo antidiabetic potential was determined in alloxan-induced diabetic mice to assess glycated hemoglobin(HbA1c),oral glucose tolerance,serum amylase,lipid profile,fasting blood glucose,and body *** lesions of the pancreas,liver and kidney were *** stress biomarkers such as superoxide dismutase,catalase and peroxidase were also ***:Quercetin,chlorogenic acid,p-coumaric acid,and m-coumaric acid were found in the plant *** methanolic plant extract exhibited higher in vitro antioxidant activities than the ethyl acetate ***,methanolic plant extract exhibited(83.90±1.56)%α-amylase inhibitory activity at 3.2 mg/mL *** study showed that the methanolic extract of *** improved the levels of fasting blood glucose,HbA1c,serumα-amylase,lipid profile,liver function biomarkers,and kidney functions of diabetic ***,the methanolic extract ameliorated diabetes-related oxidative stress by increasing superoxide dismutase and catalase activities and decreasing peroxidase and malondialdehyde *** examination showed that the plant extract had improved the integrity of pancreatic islets of Langerhans and reduced the pathological lesions in the liver and kidney of diabetic ***:The methanolic extract of *** exhibits pronounced antidiabetic activity in mice through reduction of oxidative *** plant extract has several natural antioxidants such as phenolic *** extract could serve as a nutra

关键词： Tylophora hirsuta Diabetes mellitus Oxidative stress Alpha-amylase Phenolic compounds Pancreas